APPENDICES

Water sample Collection in Bokbok Spring, Carcar.

The three agars used for testing

Weighing of the agars

Culture Media Preparation

Culture media ready for sterilization

Sterilizing the water samples using an autoclave for an hour.

 After incubation for 1 week, ready for colony counting

Colony Counting
Gram-staining

Prepared slides for coliform testing after gram-staining

Dilution

Incubation
Multiple Tube Fermentation Technique

Pour Plate Method

Streaking
Results with different Concentrations