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https://doi.org/10.1007/s11101-018-9580-2
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Phytochem Rev
2013). Chalcone isomerase (CHI) is responsible for This review focuses on stilbenes as a specific class
the conversion of chalcone to flavanones. On the other of non-flavonoid phenolic compounds present in
hand, resveratrol O-methyltransferase (ROMT) is edible berries. The aim of this review is to summarize
involved in the methylation of resveratrol. Stilbenes the isolation and identification methods applied for
are biosynthesized and accumulate into lipid vesicles stilbenes present in edible berries as well as the
in the cytoplasm. Their content can be determined by influence of external stimuli on the quantitative and
many factors, including the cultivar, ripening stage, qualitative composition of stilbenes in edible berries.
climatic conditions, soil type, agronomic manage-
ment, storage conditions and postharvest management
(Dixon and Paiva 1995; Castrejon et al. 2008). Due to Molecular structures of stilbenes found in berry
the potential health benefits, stilbenes in edible fruits fruits
are of high interest. These compounds have demon-
strated a wide range of biological and pharmacological The stilbene structure is characterized by two aromatic
activities, including anti-tumoural (Bai et al. 2010; rings linked by a double bond, of which the E isomer is
Tsai et al. 2017), anti-viral (Nguyen et al. 2011), anti- the most common configuration. They can be found in
inflammatory (Zhang et al. 2010), anti-atherogenic berry fruits as monomers, dimers and more complex
(Ramprasath and Jones 2010), anti-aging (Kasiotis oligomers (Figs. 2, 3, 4, 5, 6, 7). According to a current
et al. 2013) and neuroprotective (Lin and Yao 2006) paper, the most widely found monomeric stilbenes in
effects. berry fruits are E-resveratrol (1) and E-piceid (3)
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Fig. 4 Molecular structures of stilbene dimers isolated from 15: pallidol, 16: pallidol-3-O-glucoside, 17: ampelopsin D, 18:
edible berries: 10: E- and Z-e-viniferin, 11: E- and Z-d-viniferin, caraphenol B, 19: ampelopsin B
12: E- and Z-x-viniferin, 13: scirpusin B, 14: parthenocissin A,
extracted stilbenes was examined. Based on Sun et al. Therefore, the preparation procedure of stilbenes
work (Table 1, entry no. 15), methanol acidified with isolation should be carried out in the dark due to the
0.1% HCl was the best solvent to extract specific light sensitivity of double bond in stilbenes.
stilbenes from grape skins and seeds. In the research Few studies have dealt with the influence of
conducted by Romero et al. (2001) the influence of ultrasound on the resveratrol extraction efficiency
temperature and time of extraction on stilbenes from grapes (Burin et al. 2014; Babazadeh et al. 2017).
content was examined. The highest extraction of E- The ultrasonication-assisted extraction of resveratrol
resveratrol and piceid isomers was observed at 60 °C showed more efficiency than the conventional solvent
for 30 min with 80% ethanol. Z-Resveratrol was not extraction with 80% ethanol at 60 °C for 30 min. The
detected in any conditions assayed. The longer time of recovery of resveratrol increased by 24–30% com-
extraction at 60 °C, the lower stilbenes content was pared with the conventional solvent extraction (Cho
measured, probably due to their degradation. It is only et al. 2006).
known that resveratrol and its glycon piceid are To decrease background noise in analytical tech-
stable at 40 °C in the presence of ambient air (Prokop niques and improve the identification of stilbenes, the
et al. 2006). However E-isomer is unstable in solution purification stage in the preparation procedure is very
when exposed to light and readily isomerizes to the Z- often applied. Typically, this purification utilizes an
form and other degradants (Jensen et al. 2010). additional extraction with other solvent, often EtOAc
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Phytochem Rev
Fig. 5 Molecular structures of stilbene trimers isolated form edible berries: 20: amurensin B, 21: gnetin H, 22: vitisin E, 23: a-
viniferin, 24: miyabenol C, 25: dividol A, 26: amurensin G, 27: ampelopsin G (wilsonol B), 28: wilsonol A
(He et al. 2009a, b; Sun et al. 2006; Jiang et al. 2012), DAD/TQD) (Guerrero et al. 2010a), gas chromatog-
silica gel (Jiang et al. 2012) or C18 solid-phase raphy-mass spectrometry (GC–MS) (Ragab et al.
extraction (Kiselev et al. 2017). 2006; Viñas et al. 2009, 2011), gas chromatography–
Many analytical methods with various detection mass spectrometry with selected ion monitoring (GC–
techniques are reported for the separation and identi- MS SIM) (Rimando and Cody 2005), gas–liquid
fication of individual stilbenes in edible berries, such chromatography with flame ionization detection
as liquid chromatography-tandem mass spectrometry (GLC-FID) (Moriartry et al. 2001), capillary elec-
(LC–MS/MS) (Vrhovsek et al. 2012), liquid chro- trophoresis (CE) (Ehala et al. 2005) and high-speed
matography-mass spectrometry (LC–MS) (Može et al. counter-current chromatography (HSCC) (He et al.
2011), liquid chromatography with dual detection by a 2009b). Various methods are used for analyses of
photodiode array and quadrupole time-of-flight mass stilbenes contents in the edible berries are different
spectrometry (LC-PDA-QTOF/MS) (Samoticha et al. which could also contribute to the observed variability
2017), high-pressure liquid chromatography-mass in the published results. However, the most commonly
spectrometry (HPLC–MS) (Bavaresco et al. 2002; used methods for stilbenes analysis in different edible
Jiang et al. 2012; Kiselev et al. 2017), high-pressure berries is normal- and reverse-phase liquid chro-
liquid chromatography with diode array detection matography connected to a diode array detector
(HPLC–DAD) (Bavaresco et al. 2002; Sun et al. 2006; (DAD) or mass spectrometry (MS). To improve the
Vilanova et al. 2015; Guerrero et al. 2010a, 2016), identification of stilbenes the ultra-performance LC
high-pressure liquid chromatography with UV detec- (UPLC) technique coupled with QTOF-MS has been
tion (HPLC–UV) (Vincenzi et al. 2013; Kawakami used due to higher resolution and sensitivity of
et al. 2014; He et al. 2009a), ultra-high-pressure liquid analysis (Flamini et al. 2016) (Table 1, entry no. 24,
chromatography quadrupole time-of-flight mass spec- 26, 27).
trometry (UHPLC/QTOF/MS) (Flamini et al. 2016; Gas chromatography coupled with mass spectrom-
De Rosso et al. 2016), ultra-performance liquid etry (GC–MS) has also been applied for the analyses
chromatography with dual detection by a photodiode of stilbenes (Ragab et al. 2006; Rimando and Cody
array and fluorescence detectors (UPLC/DAD/FL) 2005; Viñas et al. 2009, 2011). Prior to GC–MS, the
(Samoticha et al. 2017), ultra-performance liquid derivatization of hydroxy groups in stilbenes was
chromatography with dual detection by a diode array performed in order to reduce polarity and increase
and tandem quadrupole mass spectrometry (UPLC/ volatility, and simultaneously, thermal stability of
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Phytochem Rev
OH OH
OH OH OH
O
HO HO HO
H H H
H H
OH H H
HO HH H
H O O O O
H HO HO
OH
OH OH
OH
H O H O H O
OH OH OH
HO H HO H HO H
HO OH HO OH HO OH
OH HO
OH OH HO OH
HO
HO H
H HO H HO H OH
HO HO H
OH O
H H H H H H
O O
H
O H O H H
H H H H HO OH OH
OH OH OH OH
H H
O
HO OH HO OH H
HO HO
HO
HO
OH OH H
O
OH
HO HO OH
H OH H HO H OH
HO OH H
HO
H OH O H H
O H HO
HO H HO OH
H H O
H
H O H
HO H O HO
OH H OH
O
HO O H
HO
HO HO
OH
34 [C56H 42O12, MW 906.94] OH
35 [C56H 42O12, MW 906.94] 36 [C56H 42O12, MW 906.94]
Fig. 6 Molecular structures of stilbene tetramers isolated form edible berries: 28: vitisin A, 29: vitisin B, 30: vitisin C, 31:
hopeaphenol, 32: isohopeaphenol, 33: vaticanol C, 34: wilsonol C, 35: heyneanol A, 36: diviniferin B
metabolites. Stilbenes derivatization was based on was performed by IR, MS, UV–Vis and NMR
silylation reactions by means of N,O-bis(trimethylsi- methods.
lyl)trifluoroacetamide (BSTFA) or N-methyl-N- It is well known that the highest concentrations of
(trimethylsilyl)-trifluoroacetamide (MSTFA) (Ri- stilbenes are in berries seeds and skins (Sun et al.
mando and Cody 2005; Ragab et al. 2006; Viñas 2006; Babazadeh et al. 2017). The amount of E-
et al. 2009, 2011). However, due to the lower resveratrol in grape skin is approximately three times
detectability of this technique only mono-stilbenes higher than in pulp (Babazadeh et al. 2017). Among
were determined such as E- and Z-resveratrol, E- and the analysed edible berries, the highest concentration
Z-piceid, pterostilbene and piceatannol. of stilbenes was found in the seeds of passion fruit
Two strategies have been applied to identify (Passiflora edulis) (Kawakami et al. 2014) by apply-
stilbenes. In the case of known compounds, the ing 80% EtOH as the solvent for extraction. However,
identification was based on comparison of their the highest concentration of E-resveratrol in the skin
retention times and MS or MS/MS data with those of has been found in table grapes (Vitis vinifera L.)
standards. For unknown stilbenes, the characterization (Ragab et al. 2006) by using extraction with ethyl
acetate at 70 °C.
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Table 1 Preparation conditions and analytical methods for stilbene separation and identification from edible berries
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
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Phytochem Rev
Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
5 Highbush blueberry Method used for berries from GC–MS Bluecrop from Rimando
(Vaccinium corymbosum L.) US location SIM conventional and Cody
Cultivars: 1. Material: lyophilized berries farming (2005)
Bluecrop 2. Solvent: MeOH/acetone/H2O/ Resveratrol:
CH3COOH (40:40:20:0.1) 0.853 lg/g dw
wild
3. Conditions: 40 °C, 1000 psi Piceatannol:
0.422 lg/g dw
4. Purification: extraction with
EtOAc Bluecrop from sustainable
farming
5. Derivatization: MSTFA/DFA/
MeOH (3.5:1:0.5) Resveratrol:
0.327 lg/g dw
Method used for berries from
Canada Piceatannol:
0.186 lg/g dw
1. Material: frozen berries
Wild
2. Solvent: MeOH/acetone/H2O/
HCOOH (40:40:20:0.1) Resveratrol:
1.074 lg/g dw
3. Conditions: room temperature
4. Purification: C18 solid-phase
extraction
5. Derivatization: MSTFA/DFA/
MeOH (3.5:1:0.5)
6 Rabbiteye blueberry Method used for berries from GC–MS Tifblue Rimando
(Vaccinium ashei Reade) US location SIM Resveratrol: and Cody
Cultivars: 1. Material: lyophilized berries 0.106 lg/g dw (2005)
Tifblue 2. Solvent: MeOH/acetone/H2O/ Pterostilbene:
Climax CH3COOH (40:40:20:0.1) 0.151 lg/g dw
Premier 3. Conditions: 40 °C, 1000 psi Climax
4. Purification: extraction with Resveratrol:
EtOAc 0.390 lg/g dw
5. Derivatization: MSTFA/DFA/ Pterostilbene:
MeOH (3.5:1:0.5) 0.099 lg/g dw
Method used for berries from Premier
Canada Resveratrol:
1. Material: frozen berries 0.007 lg/g dw
2. Solvent: MeOH/acetone/H2O/
HCOOH (40:40:20:0.1)
3. Conditions: room temperature
4. Purification: C18 solid-phase
extraction
5. Derivatization: MSTFA/DFA/
MeOH (3.5:1:0.5)
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
10 Deerberry Method used for berries from US location GC–MS Wild Rimando
(Vaccinium 1. Material: lyophilized berries SIM Resveratrol: 0.204 lg/g dw and
stamineum L.) Cody
2. Solvent: MeOH/acetone/H2O/ SHF3A-2-108
(2005)
Cultivars: CH3COOH (40:40:20:0.1) Resveratrol: 0.115 lg/g dw
wild 3. Conditions: 40 °C, 1000 psi Pterostilbene: 0.520 lg/g dw
SHF3A-2-108 4. Purification: extraction with EtOAc B-76
B-76 5. Derivatization: MSTFA/DFA/MeOH Resveratrol: 0.503 lg/g dw
(3.5:1:0.5)
Piceatannol: 0.195 lg/g dw
Method used for berries from Canada
1. Material: frozen berries
2. Solvent: MeOH/acetone/H2O/HCOOH
(40:40:20:0.1)
3. Conditions: room temperature
4. Purification: C18 solid-phase extraction
5. Derivatization: MSTFA/DFA/MeOH
(3.5:1:0.5)
11 Table grapes 1. Material: fresh skin GLC-FID Resveratrol Moriartry
(Vitis vinifera L.) 2. Solvent: MeOH/0.1% HCl Black Corinth cultivar et al.
(2001)
Californian 3. Conditions: room temperature Non-irradiated fruits:
cultivars: 4. Purification: extraction with EtOAc 0–25.1 lg/g fw
Black Corinth Irradiated fruits: 0.9–33.2 lg/g fw
Flame Seedless Flame Seedless cultivar
skin Non-irradiated fruits:
1–13.2 lg/g fw
Irradiated fruits: 1.8–57.3 lg/g fw
12 Grapes 1. Material: fresh berries (without seeds) HPLC– E-resveratrol: 0.297 lg/g fw Bavaresco
(Vitis vinifera L.) 2. Solvent: 95% MeOH DAD E-piceid: 0.097 lg/g fw et al.
HPLC–MS (2002)
Cultivar: 3. Conditions: room temperature Piceatannol: 0.052 lg/g fw
Cabernet 4. Purification: EtOAc/5% NaHCO3 (1:1) Z-resveratrol: nd
sauvignon Z-piceid: nd
13 Grapes Method used for berries from US location GC–MS Cabernet Rimando
(Vitis vinifera L.) 1. Material: lyophilized berries SIM Resveratrol: 2.475 lg/g dw and
Cody
Cultivars: 2. Solvent: MeOH/acetone/H2O/ Pinot Noir
(2005)
Cabernet CH3COOH (40:40:20:0.1) Resveratrol: 5.746 lg/g dw
Pinot Noir 3. Conditions: 40 °C, 1000 psi Merlot
Merlot 4. Purification: extraction with EtOAc Resveratrol: 6.356 lg/g dw
Table grapes 5. Derivatization: MSTFA/DFA/MeOH Table grapes
(3.5:1:0.5)
Resveratrol: 6.471 lg/g dw
Method used for berries from Canada
1. Material: frozen berries
2. Solvent: MeOH/acetone/H2O/HCOOH
(40:40:20:0.1)
3. Conditions: room temperature
4. Purification: C18 solid-phase extraction
5. Derivatization: MSTFA/DFA/MeOH
(3.5:1:0.5)
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
14 Table grapes 1. Material: lyophilized tomato or grape skin GS-MS Z-resveratrol: 20 lg/g dw Ragab
(Vitis 2. Solvent: EtOAc E-resveratrol: 2680 lg/g dw et al.
vinifera (2006)
3. Conditions: 70 °C Z-piceid: 30 lg/g dw
L.)
4. Derivatization: MSTFA/pyridine E-piceid: 50 lg/g dw
seedless red
15 Grapes Method I HPLC– Method I Sun et al.
(Vitis 1. Material: lyophilized grape skin DAD E-resveratrol: * 40 lg/g fw (2006)
vinifera 2. Solvent: MeOH, 80% MeOH/H2O, 50% E-piceid: * 70 lg/g fw
L.) MeOH/H2O, 75% acetone/H2O Z-piceid: * 130 lg/g fw
Cultivars: 3. Conditions: room temperature Method II
Castelao 4. Purification: extraction with EtOAc E-resveratrol: * 40 lg/g fw
Syrah Method II E-piceid: * 10 lg/g fw
Tinta Roriz 1. Material: lyophilized grape skin Z-piceid: * 20 lg/g fw
seeds 2. Solvent: EtOAc Method III
skin 3. Conditions: room temperature E-resveratrol: * 80 lg/g fw
Method III E-piceid: * 120 lg/g fw
1. Material: lyophilized grape skin Z-piceid: * 170 lg/g fw
2. Solvent: MeOH Method IV
3. Conditions: room temperature E-resveratrol: * 100 lg/g fw
4. Purification: extraction with EtOAc E-piceid: * 140 lg/g fw
Method IV Z-piceid: * 200 lg/g fw
1. Material: lyophilized grape skin Method V
2. Solvent: MeOH/0.1% HCl E-resveratrol: * 30 lg/g fw
3. Conditions: room temperature E-piceid: * 20 lg/g fw
4. Purification: extraction with EtOAc Z-piceid: * 130 lg/g fw
Method V Method VI
1. Material: lyophilized grape skin E-resveratrol: * 10 lg/g fw
2. Solvent: 75% acetone/H2O E-piceid: * 10 lg/g fw
3. Conditions: room temperature Z-piceid: * 30 lg/g fw
4. Purification: extraction with EtOAc Method IV
Method VI Seed
1. Material: lyophilized grape skin Total resveratrol:
2. Solvent: model wine solution (12% of EtOH, 4.76 ± 0.25 lg/g dw
5 gL-1 of l-tartaric acid, pH 3.2) Total piceid: nd
3. Conditions: room temperature Skin
4. Purification: extraction with EtOAc Total resveratrol:
208.50 ± 51.97 lg/g dw
Total piceid:
762.47 ± 164.16 lg/g dw
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
16 Southeast China grape 1. Material: fresh grape HPLC–UV Chunganenol: 8.57 lg/g fw He et al.
(Vitis chunganensis) 2. Solvent: MeOH Amurensin B: 242.86 lg/g fw (2009a)
3. Conditions: room Gnetin H: 131.43 lg/g fw
temperature e-viniferin: 154.29 lg/g fw
4. Purification: EtOAc and Amurensin G: 262.86 lg/g fw
silica gel/EtOAc/light
Vitisin A: 191.43 lg/g fw
petroleum
Hopeaphenol: 88.57 lg/g fw
Resveratrol: 48.57 lg/g fw
17 Southeast China grape 1. Material: dried fruits HSCCC Hopeaphenol: 84.4 lg/g dw He et al.
(Vitis chunganensis) 2. Solvent: MeOH Amurensin G: 148.8 lg/g dw (2009b)
3. Conditions: room Vitisin A: 382.4 lg/g dw
temperature
4. Purification: extraction with
EtOAc
18 Grapes 1. Material: fresh grapes GC–MS Red grapes Viñas et al.
(Vitis vinifera L.) 2. Solvent: EtOH E-resveratrol: 0.029 lg/g fw (2009)
red and white 3. Conditions: sonication at Z-resveratrol: 0.0028 lg/g fw
room temperature Piceatannol: 0.024 lg/g fw
4. Derivatization: BSTFA White grapes
E-resveratrol: 0.0056 lg/g fw
Z-resveratrol: nd
Piceatannol: 0.0012 lg/g fw
19 Grapes 1. Material: frozen grapes UPLC-DAD- Merlot: non-UV, UV-C treatment Guerrero
(3 Vitis vinifera 2. Solvent: Et2O TQD (lg/g fw) et al.
sylvestris, identification E-resveratrol: 2.82, 9.75 (2010a)
3. Conditions: room
7 Vitis vinifera sativa, temperature HPLC–DAD Piceatannol: 0.48, 2.55
2 Hybrid Direct determination Viniferins: 0.29, 2.39
Producers) Syrah: non-UV, UV-C treatment
(lg/g fw)
E-resveratrol: 3.56, 19.56
Piceatannol: 0.46, 0.31
Viniferins: nd, 1.49
Tempranillo: non-UV, UV-C
treatment (lg/g fw)
E-resveratrol: 0.31, 3.78
Piceatannol: nd, 1.10
Viniferins: 0.14, 1.19
20 Grapes 1. Material: fresh fruits GC–MS Red grapes Viñas et al.
(Vitis vinifera L.) 2. Solvent: undecanone E-resveratrol: 1.639 lg/g fw (2011)
red and white 3. Conditions: 30 °C Z-resveratrol: 0.405 lg/g fw
4. Derivatization: BSTFA Piceatannol: 0.374 lg/g fw
White grapes
E-resveratrol: 0.239 lg/g fw
Z-resveratrol: 0.082 lg/g fw
Piceatannol: 0.043 lg/g fw
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
27 Grapes 1. Material: fresh berries UHPLC- Raboso Piave: fresh, dried (lg/g) De Rosso et al.
(Vitis 2. Solvent: MeOH QTOF-MS a-viniferin: 0.79 ± 0.20, 1.05 ± 0.27 ( 2016)
vinifera) 3. Conditions: room temperature Z-piceid: 4.00 ± 1.32, 5.34 ± 1.76
Cultivars: E-astringin: 1.44 ± 0.30, 1.91 ± 0.39
Corvina Pallidol-3-O-glucoside: 0.91 ± 0.26,
Raboso Piave 1.21 ± 0.34
Piceatannol: 1.95 ± 0.14,
2.59 ± 0.19
Pallidol: 1.02 ± 0.15, 1.36 ± 0.21
Parthenocissin A: 0.56.0 ± 0.05,
0.57 ± 0.07
Z-e-viniferin: 1.55 ± 0.22,
2.07 ± 0.30
E-e-viniferin: 5.80 ± 1.88,
7.73 ± 2.50
d-viniferins: 0.81 ± 0.30,
1.08 ± 0.40
Z-miyabenol C: 0.61 ± 0.19,
0.82 ± 0.25
E-miyabenol C: 5.51 ± 0.87,
7.35 ± 1.16
E-piceid: 3.84 ± 1.00, 5.13 ± 1.33
E-resveratrol: 4.93 ± 0.36,
6.58 ± 0.48
Z-astringin: 0.13 ± 0.03, 0.17 ± 0.04
28 Grapes 1. Material: freeze-dried fresh HPLC–DAD After UV-C treatment (lg/g fw) Guerrero et al.
(Vitis grape skins E-resveratrol: 121.62 (2016)
vinifera L.) 2. Solvent: Et2O Z-piceid: 61.22
Cultivar: 3. Conditions: room temperature E-piceid: 15.81
Crimson E-piceatannol: 14.7
Seedless
e-viniferin: 11.25
x-viniferin: 5.8
Isohopeaphenol: 4.27
Stilbenoid: 234.67
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
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Table 1 continued
No. Sample Preparation conditions Analytical Stilbenes: concentration (lg/g) References
method
However, these studies only focused on berries at was also related to berry development. Among the six
veraison and ripening. It is known that the biosynthesis developmental stages, the stage at 55 DAA (days after
and accumulation of stilbenes in berries after UV-C anthesis, 2 weeks before veraison) was the most
treatment depends on the developmental stages of sensitive to UV-C treatment. The contents of E-
fruits. From genomic analyses, it has been deduced resveratrol and E-piceid increased 292- and 11-fold,
that there are two main stages during grape develop- respectively (Table 2, no. 3). Along with develop-
ment that are sensitive to UV-C irradiation (Pilati et al. mental factors, the sensitivity of resveratrol synthesis
2007), namely, before and after veraison. ‘‘Before to UV-C irradiation gradually declined, which may be
veraison’’ constitutes the restructuring phase of cell associated with the regulation of STS by the Myb14
metabolism, characterized by an up-regulation of promoter. STS expression was the highest when the
genes associated with hormone signalling and tran- berries were exposed to UV-C irradiation at 55 DAA,
scription. ‘‘After veraison’’ is characteristic of fruit which may explain why resveratrol accumulated
ripening, whereas ‘veraison’ is characterized by an during this developmental stage. The expression of
oxidative burst and antioxidant regulation. Therefore, the Myb14 promoter reached maximum levels 12 h
it has been speculated that ‘veraison’ may be the most before STS. These results suggest that Myb14 expres-
sensitive stage in which to apply UV-C treatment. sion may play an important role in the transcriptional
Under natural conditions, before veraison, the E- regulation of resveratrol biosynthesis induced by UV-
resveratrol content was very low in ‘Beihong’ (V. C irradiation.
vinifera 9 V. amurensis) berries (Wang et al. 2015).
From veraison to maturity, the E- and Z-piceid
contents increased. UV-C treatment significantly
stimulated the biosynthesis of E-resveratrol and E-
piceid. The response of berries to UV-C irradiation
123
Phytochem Rev
Table 2 Influence of abiotic and biotic external stimuli on the presence of stilbenes in grapes
E-resveratrol: 14.4 ↑
Postharvest storage: 3 days, at 20oC, 80% RH
ε-viniferin: 3.4 ↑
123
Phytochem Rev
Table 2 continued
60 s, 90 W 10 ↑
60 s, 240 W 10.5 ↑
60 s, 510 W 5↑
300 s, 30 W 9.4 ↑
300 s, 90 W 8↑
300 s, 240 W 6.5 ↑
300 s, 510 W
7 Redglobe table UV-C treatment Dose: 0.8, 2.4, 4.1 kJ/m2 (Crupi et
grape Distance: 40 cm al. 2013)
(Vitis vinifera Treatment time: 3 min, storage time: 48 h at 4oC E-piceid: 1.2 ↑
L.) Z-piceid: 2.6 ↑
o
Treatment time: 3 min, storage time: 48 h at 25 C
E-piceid: 0.1 ↑
Z-piceid: 0.5 ↑
8 Red grapes UV-C treatment Power: 1020 W Syrah, terroir Jerez: (Fernández
(Vitis vinifera Distance: 42 cm resveratrol: 2.7 ↑ -Marín et
L.): Time: 60 s piceatannol: 6.1 ↑ al. 2013)
viniferin: 1.6 ↑
Syrah, Merlot, Storage conditions: total stilbenes: 3.1 ↑
Cabernet at 20oC for 7 days, 80% RH
sauvignon, Syrah, terroir Cabra:
Pinot noir resveratrol: 1.1 ↑
piceatannol: 3.5 ↑
viniferin: 2 ↑
total stilbenes: 1.5 ↑
9 Pinot Noir UV-C treatment Distance: 50 cm E-resveratrol: 355 ↑ (Suzuki et
(Vitis vinifera Light intensity: 0.25 μW/cm2 ε-viniferin: 4.8 ↑ al. 2015)
L.) Time: 1 h piceid: 2.4 ↑
Storage conditions: Z-resveratrol: 0.7 ↑
23 h in the dark at 25oC
10 Red Globe UV treatment Storage conditions: 4oC during 4 weeks E-resveratrol: (Jiménez
grapes Treatment time: 30 min: R 0.8 ↑ Sánchez et
(Vitis vinifera 302.1 nm resonant wavelength (R) Treatment time: 30 min: NR 0.3 ↓ al. 2007)
L.) 300.0 nm non-resonant wavelength Treatment time: 45 min: R 6.2 ↑
(NR) Treatment time: 45 min: NR 0.1 ↑
Treatment time: 60 min: R 0.4 ↓
Treatment time: 60 min: NR =
11 Crimson red UV-C treatment and 0.5%/1% Light intensity: 2.82 mW/cm2 (Freitas et
table grapes chitosan coating (CHT) Distance: 60 cm al. 2015)
(Vitis labrusca) Temperature: 10oC
E-resveratrol:
Treatment with UV-C: 0.4 ↑
Treatment with UV-C and storage 20oC/24 h: 3↑
Treatment with CHT 0.5%: =
Treatment with UV-C, CHT 0.5%, storage 5 days: 2↑
Treatment with UV-C, CHT 0.5%, storage 20oC/24 h, 5
days: 4.9 ↑
Treatment with UV-C, CHT 0.5%, storage 8 days: 2.9 ↑
Treatment with UV-C, CHT 0.5%, storage 20oC/24 h, 8
days: 3.7 ↑
12 Beihong Treatment with UV and CaCl2 Light intensity: 6 W/m2 (Wang et
(Vitis Distance: 15 cm al. 2013)
vinifera × V. Time: 10 min
amurensis) Storage conditions:
Hongbaladuo at 25oC in the dark for 24 h, then at -1oC for 27 days, RH
95%
Treatment with CaCl2: E-resveratrol: 0.5 ↑
E-piceid: =
Z-piceid: 0.2 ↓
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Phytochem Rev
Table 2 continued
123
Phytochem Rev
Table 2 continued
ε-viniferin: nd
4 days after treatment:
E-resveratrol: 0.5 ↑
piceatannol: 0.8 ↑
isorphapontigenin: 0.5 ↑
ε-viniferin: 1 ↑
at harvest:
E-resveratrol: 1 ↑
piceatannol: nd ↑
isorphapontigenin: nd
ε-viniferin: nd
2) Postharvest treatment with UV-C:
2 days after treatment:
E-resveratrol: =
piceatannol: =
isorphapontigenin: nd
ε-viniferin: nd
4 days after treatment:
E-resveratrol: 0.5 ↑
piceatannol: 0.4 ↑
isorphapontigenin: 0.7 ↑
ε-viniferin: 0.4 ↑
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Phytochem Rev
Table 2 continued
Damaged grape:
treatment with UV-C, infection, storage 5 days: E-resveratrol: 6 ↑
E-piceid: 1 ↑
treatment with infection, UV-C, storage 5 days: E-resveratrol: 4 ↑
E-piceid: 0.5 ↑
nd: not detected
= no changes, ↑ increase, ↓ decrease
Abiotic postharvest treatments of grape in skin, respectively, which was approximately three-
fold higher than those in control berry samples.
Postharvest treatment with UV-C light has been Similar results were found in Napoleon table grapes
proposed as a valuable method to increase the (Cantos et al. 2001). Cold storage in combination with
stilbenes content in the grape berries (Langcake and UV irradiation increased the piceid concentration
Pryce 1977; Douillet-Breuil et al. 1999; Versari et al. more than cold storage alone. Also Cho et al. (2012)
2001; Petit et al. 2009; Yin et al. 2016). reported that it is possible to enrich resveratrol content
Postharvest treatment of grape varieties with UV-C in harvested grapes by modulating cell metabolism
resulted in higher concentrations of stilbenes, such as with UV treatment and storage conditions. Storage
E-resveratrol, piceatannol, viniferins and pterostilbene temperature had an effect on time-delayed resveratrol
(Table 2, no. 4, 5) (Adrian et al. 2000; Guerrero et al. biosynthesis after removal of the UV irradiation. A
2010a). Differences in concentration after UV-C larger amount of resveratrol was formed when UV-
irradiation depended on the variety and campaign, treated grapes were stored at higher temperature.
but not on the grape subspecies. Each variety seemed After UV-C postharvest irradiation, all of the red
to be influenced to a different degree by the climate. grape varieties in each terroir increased their resver-
Thus, the same variety behaved in a different way in atrol, piceatannol and viniferin contents (Table 2, no.
each campaign, and climate could determine the final 8) (Fernández-Marı́n et al. 2013). The stilbene content
concentration of stilbenes. was different depending on the variety and the terroir.
The highest accumulation of resveratrol (tenfold) in Cabra was the terroir where the varieties achieved the
irradiated Napoleon grapes was achieved using the highest induction capacity (2.02 lg/g per day after
following combination of parameters: irradiation UV-C irradiation), especially the Syrah variety. This is
power, 510 W; irradiation time, 30 or 60 s; irradiation in agreement with previous research in which Syrah
distance, 40 cm; and elapsed days, 3 (Table 2, no. 6). increased its stilbene content more than the other
Therefore, controlled UV irradiation parameters are thirteen varieties studied (Guerrero et al. 2010a).
useful as a simple postharvest treatment to increase the However, the highest increase in the resveratrol and
resveratrol concentration in Napoleon grapes (Cantos piceatannol contents in the Syrah variety was from the
et al. 2001). Jerez terroir, which amounted to 2.7 and 6.1 times,
To achieve the highest possible stilbene accumu- respectively, in comparison to those in the untreated
lation, the interactive effects of storage time, temper- berries.
ature and UV-C irradiation on the stilbene content in With regard to piceatannol and viniferins, higher
postharvest Red globe table grapes were investigated concentrations were found in varieties that achieved
(Table 2, no. 7) (Crupi et al. 2013). During storage, higher resveratrol levels because resveratrol has been
both cold storage and UV-C doses of 3 min raised the proposed as the precursor of the other stilbenes
contents of Z- and E-piceid, achieving 90 and 34 lg/g (Coutos-Thévenot et al. 2001). Thus, it could be
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Phytochem Rev
concluded that resveratrol determines the tendency for higher content of E-resveratrol and lower susceptibil-
the synthesis of other stilbenes and the amount of total ity to fungal decay than control grapes.
stilbenes. The individual and combined effects of CaCl2 and
Moreover, varieties reached the highest level of ultraviolet light on the biosynthesis of resveratrol in
resveratrol induction in different periods of time berry skins were investigated (Table 2, no. 12) (Wang
depending on the terroir. The day of maximum et al. 2013). CaCl2 application had no effect on the E-
concentration (dm) was constant for Cabernet sauvi- piceid content and little influence on the Z-piceid
gnon (dm = 7). Pinot noir showed the same dm in all content, but it resulted in a 0.5 times higher content of
terroirs (dm = 6), except for Cadiar, where it was E-resveratrol compared with that in the control
delayed 1 day (dm = 7). However, in Syrah and sample. UV-C and UV-C irradiation with CaCl2
Merlot varieties, the dm changed depending on the treatment increased E-resveratrol and E-piceid
terroir, ranging from 4 to 7 days. biosynthesis and accumulation in berry skins. This
Treatment of the postharvested Pinot Noir grape accumulation continued until 13 days after cold
berries with UV-C irradiation increased the E-resver- storage. Then, the E-resveratrol content slightly
atrol, e-viniferin, piceid and Z-resveratrol contents decreased but still remained at high levels at the end
355, 4.8, 2.4 and 0.7 times, respectively, in compar- of storage, while the E-piceid content increased
ison with those in the control sample (Table 2, no. 9) continuously until the end of storage. In contrast, the
(Suzuki et al. 2015). Transcriptome analysis revealed Z-piceid content after UV-C and UV-C/CaCl2 treat-
that 238 genes were up-regulated more than fivefold in ments decreased by 0.6 and 1.4 times, respectively,
grape berry skin by UV-C treatment. Enrichment compared with that in the control sample.
analysis of the gene ontology terms showed that genes UV-C and UV-C/CaCl2 treatments significantly
encoding stilbene synthase were enriched in the up- stimulated the expression of PAL, C4H, 4CL and STS,
regulated genes. which are related to the biosynthesis of E-resveratrol.
Two different wavelengths were used for the UV Moreover, the expression levels of these genes in the
treatment of red grapes: 302.1 nm for the resonant UV-C/CaCl2 combination treatment were higher than
wavelength and 300.0 nm for the non-resonant wave- those in the UV-C irradiation treatment. The expres-
length (Table 2, no. 10) (Jiménez Sánchez et al. 2007). sion of PAL, C4H, 4CL, and STS in both treatments
Four sets of irradiation times were selected for each of reached a maximal content at 12 h after initiating the
the two different wavelengths: 15, 30, 45 and 60 min. treatment and then declined rapidly to approach the
The use of photons of resonant energy to produce control level at the end of the experiment. CaCl2
absorption through the real electronic states of the treatment alone did not modify the expression of any
molecule significantly increased the absorption yield, of these genes (Wang et al. 2013).
producing an important effect on the photoinduced E- Increased accumulation of E-resveratrol in grape
resveratrol level in the grapes. The enhancement was skin by ultrasonication treatment has also been
optimal for 45 min of irradiation at 302.1 nm. The investigated (Table 2, no. 13) (Hasan and Baek
samples were prepared for analysis immediately after 2013). A significantly higher amount of E-resveratrol
irradiation to detect the direct effect of resonant over that in the control sample was observed following
elicitation. ultrasonication treatment. Ultrasonic treatment for
The combination of UV-C treatment with a chi- 5 min followed by 6 h of incubation induced the
tosan coating and incubation was investigated highest levels of E-resveratrol, with amounted to a
(Table 2, no. 11) (Freitas et al. 2015). The concentra- level that was 6.7 times higher than that in the control
tion of E-resveratrol in red table grapes with a 0.5% sample. However, the treatment did not lead to an
chitosan coating treated with UV-C irradiation, incu- increase in the maintenance of the level of E-
bated at 20 °C and then stored for 5 days under resveratrol. When the amount of E-resveratrol was
refrigeration was approximately 5 and 2.5 times higher measured again in fruits incubated for another 6 h, it
than that in control and UV-C treated grapes with a had decreased drastically to the level in samples
0.5% chitosan coating, respectively. After 8 days of treated by 5 min of ultrasonication without any
storage at 4 °C, treated berries also showed a 2.9 times incubation.
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Phytochem Rev
In grape skin, the expression levels of resveratrol treatments, probably because these treatments induced
synthase increased directly in response to 5 min of a faster biosynthesis of this compound. The increases
ultrasonication treatment and were then maintained were more marked when clusters were stored at 15 °C,
during 12 h of incubation. This suggests that the which is in agreement with previous reports, where
accumulation of E-resveratrol in grape in response to control and UV-treated cv. Napoleon table grapes
ultrasonication occurs in a time-dependent manner increased their resveratrol content after the grapes
based on the induction of the resveratrol synthase were transferred to 15 °C (Cantos et al. 2000).
gene. However, O3-treated clusters that underwent shock
Ozone was also found to stimulate the synthesis of treatment increased their E-piceid content by 11 and
stilbenes in grape berries (Sarig et al. 1996; Palou et al. 4.5 times compared to that sampled at harvest.
2002). Continued and intermittent ozone treatments The accumulation of stilbenes and the expression of
(2 ppm) were applied during the storage of 3 varieties genes related to their synthesis in ‘Campbell Early’
of table grapes at 5 °C for 72 days (Table 2, no. 14) and ‘Kyoho’ grapes were investigated by irradiating
(Cayuela et al. 2010). The continuous presence of the harvested grapes with four different light sources
ozone in the storage atmosphere inhibited the biosyn- for 48 h (Table 2, no. 16) (Ahn et al. 2015). The total
thesis of resveratrol, whereas intermittent treatment concentrations of five stilbene derivatives at 24 h after
with O3 stimulated its biosynthesis. It was found that irradiation differed in response to different light
the treatment consisting of intermittent shocks of sources and cultivars. The accumulation of stilbenes
8 ppm O3 had the strongest influence on table grapes in the skins of two grape cultivars and the expression
(Artés-Hernández et al. 2003). It was also found that of PAL and STS1 genes were induced under mainly red
the grapes stored in an atmosphere containing 0.1 ppm and blue LED light. The amount of stilbenes tended to
O3 exhibited higher concentrations of resveratrol than be higher in blue and red light-treated grapes than in
those kept in air. Mild ozone treatments, such as those the grape berries treated with white fluorescent or
using ozonized water, which are effective in the purple light. Among the stilbenes tested, E-resveratrol
control of microbial growth, did not induce stilbenoid was present in ‘Campbell Early’ berries treated with
accumulation in grapes (Gonzalez Ureña et al. 2003). blue, red, purple and fluorescent light at levels 7.4-,
Continuous exposure of the berries to a high concen- 6.2-, 2.8- and 1.8-fold higher than the levels in the
tration of O3 could reduce the content of antioxidant control sample, respectively. The expression of PAL,
compounds such as resveratrol produced by the plant CHS, CHI, STS1, STS12 and ROMT genes was
as a defensive metabolite against oxidative stress. differently induced in response to irradiation with
The influence of the combination of ozone and UV- different light sources in both grape cultivars. The
treatment on stilbenes content was also investigated mRNA levels of PAL and STS1 were higher than those
(González-Barrio et al. 2006). The results showed that of CHS, CHI, STS12 and ROMT in the two grape
UV-C was generally much more efficient (shorter berries. The results indicated that red and blue LEDs
treatment time) in inducing resveratrol content than induced the accumulation of stilbenes and the expres-
ozone. However, with regard to total stilbenoids sion of genes related to their syntheses in grape
accumulated in the grape skin, the ozone treatment berries.
with the highest concentration and the longest time led Accumulation of E-resveratrol in post-harvested
to higher stilbenoid content than the UV-C irradiation. grapes by dry nitrogen treatment was also investigated
Also the viniferin content accumulated was threefold (Table 2, no. 17) (Jiménez et al. 2007). E-resveratrol
higher than that induced by the UV-C treatment. content in the grape berries increased with the duration
However, UV-C light treatment resulted in less of the dry nitrogen treatment up to 24 h (twofold). For
damage to grape tissues than ozone gas. longer treatment, only a slight enhancement was
Different gaseous treatments have been applied observed. However, shorter treatments kept higher
because of their efficacy in ensuring the quality of content of E-resveratrol during several days with no
Napoleon table grapes (Table 2, no. 15) (Artés- appreciable damage of the grape berries regarding to
Hernández et al. 2003). The E-resveratrol content their organoleptic quality.
increased up to threefold its value sampled at harvest It was also observed that storage conditions influ-
in O3 shock-treated clusters and up to twofold for other ence the stilbene concentration (Table 2, no. 18).
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Phytochem Rev
Grapes stored under mild conditions (20 °C) favoured samples, whereas MEJA-UV-C achieved the highest
E-resveratrol synthesis, whereas cold storage (4 °C) E-resveratrol content. In fact, UV-C irradiation has
decreased its concentration. Cold storage apparently been described as a stronger stress than MEJA,
inhibited E-resveratrol synthesis after harvest, and its causing higher E-resveratrol induction in grape, but
concentration slowly decreased. This may be a longer grape storage period was required (Fernán-
explained by the action of peroxidase enzymes and dez-Marı́n et al. 2012).
the subsequent formation of e-viniferin. In non-UV-C The accumulation of piceatannol, isorhapontigenin,
irradiated grapes, the E-resveratrol content increased and e-viniferin were also induced (Table 2, no. 19). At
by nearly 14 times when after 3 days of storage at low harvest, piceatannol was found in MEJA grapes, but it
temperature, the grapes were stored under mild storage was absent in control samples. After 2 days of storage,
conditions. piceatannol was found in every batch, and isorhapon-
After 60 days of withering, Raboso Piave and tigenin was found in the MEJA and MEJA-UV-C
Corvina grape samples showed an evident increase in batches in concentrations that were not significantly
the content of most stilbenes (Table 2, no. 18) (De different. The trend of these stilbenes was similar to
Rosso et al. 2016). Raboso Piave had a statistically that of E-resveratrol. The control batch showed the
significant increase in E-resveratrol, pallidol, and E-e- lowest concentration, in contrast to MEJA-UV-C,
viniferin. In Corvina grape, E-resveratrol, piceatannol, which showed the highest concentration after 4 days
E-astringin, E-piceid, pallidol, pallidol glucoside, E- of storage.
miyabenol C, and e-viniferin increased significantly. The effectiveness of the chitosan treatment of
In general, these findings are in agreement with those table grapes (Autumn Black and B36-55 variety) in
reported in a study of Aleatico grape withering combination with UV-C irradiation to determine the
(Mencarelli et al. 2010). E-resveratrol concentration in grape berry skins was
investigated (Table 2, no. 20) (Romanazzi et al. 2006).
Grape berries were sprayed in the vineyard with 1%
Combined abiotic pre- and postharvest treatments chitosan and then harvested daily for 5 days. Imme-
of grape diately after harvest, they were inoculated with B.
cinerea. In cv. Autumn Black and selection B36-55, E-
Preharvest red grapes (Syrah Vitis vinifera L.) treated resveratrol was not detected in control berries or
with methyl jasmonate (MEJA) showed a significant berries with a chitosan coating. In berries exposed to
increase in E-resveratrol and piceatannol contents UV-C irradiation alone and in berries treated with 1%
compared to those in the control sample (piceatannol chitosan coating and later exposed UV-C, E-resvera-
not detected) (Table 2, no. 19) (Fernández-Marı́n et al. trol was found. The berries treated with the combina-
2014). The highest concentrations of piceatannol and tion of chitosan and UV-C irradiation contained
E-resveratrol were found at harvest. Larronde et al. approximately 11 times more E-resveratrol than the
(2003) described an E-resveratrol increase (ninefold) control grapes and 0.2 times more than those only
in Cabernet sauvignon berries when treated with irradiated with UV-C. Z-Resveratrol, E-piceid, and Z-
MEJA vapours 15 days after veraison. However, piceid were not detected in any of the samples.
berries rapidly lose the capacity to respond to MEJA
with ripening. Vezzulli et al. (2007) found that MEJA
treatment of the Barbera grape variety improved the E- Biotic postharvest treatments of grape
resveratrol and e-viniferin contents in an accumulative
manner. Thus, it seems that there is an effect of MEJA Infection of the postharvested Palomino fino grapes
on the stilbene concentration in grapes. with Botrytis cinerea led to a domination of piceid
Harvested red grapes were treated with UV-C over resveratrol (Table 2, no. 21) (Roldán et al. 2003).
irradiation and stored for 4 days (Table 2, no. 19) At the early stage of fungal development, the content
(Fernández-Marı́n et al. 2014). On the 4th day, control of piceid increased more than onefold in fruits over
red grapes showed a significantly lower E-resveratrol that in the control sample, while the level of resver-
content than grapes treated with UV-C, and UV-C atrol decreased more than onefold. At more advanced
samples a lower E-resveratrol content than MEJA infection stages, the piceid and resveratrol contents
123
Phytochem Rev
increased 3.5-fold and 0.4-fold, respectively. Accord- stilbenes decreased, which confirms the low suscep-
ing to the literature, resveratrol synthesis occurs in the tibility of this cultivar against A. carbonarius
skin of edible berries (Sotheeswaran and Pasupathy infection.
1993). Thus, an accumulation of resveratrol in the skin In the research conducted by Bavaresco et al.
after infection by B. cinerea could be expected. (2003) the influence of fungal infection with A.
However, the results showed that even at the early japonicus, A. ochraceus, A. fumigatus and A. car-
stage of pathogen development, a sharp decrease in the bonarius on the stilbenes content in the grape berries
resveratrol content occurred. This could be because was examined. All tested fungi, except A. fumigatus,
before pathogen exposure, resveratrol synthesis is significantly increased E-resveratrol concentration
stimulated. The resveratrol already present in the skin over the control sample, while E-piceid content was
is used by the fruit as part of its defence mechanism. not affected. Among tested fungi, only A. ochraceus
This phytoalexin would become a building block of significantly elicited the grape berries to synthesize
stilbenes such as e- and d-viniferins, which are more piceatannol.
toxic to the pathogen. For this reason, the piceid and
resveratrol contents decreased. In the second stage of
pathogen development, an accumulation of resveratrol Combined abiotic and biotic postharvest
occurred until a certain level was reached, and it was treatments of grape
maintained during external stress. These results are in
accordance with the literature, which showed that in In another study, phenyl propanoid metabolism induc-
grape berry skins infected by powdery mildew, the tion by abiotic (UV-C) and biotic elicitors (fungal
resveratrol and piceid isomers were considerably infection—ochratoxigenic Aspergillus) in undamaged
increased and the degree of infection was positively and damaged Napoleon table grapes was investigated
related to their stilbene content (Romero et al. 2001). (Table 2, no. 23) (Selma et al. 2008). In addition, the
Infection with Aspergillus carbonarius at ripening effect of the sequence of elicitors on the content of
induced in the grapes an approximately onefold stilbenes and storage time was analysed. The contents
increase in E-resveratrol and resveratrol dimers, such of E-resveratrol in non-inoculated and inoculated
as x-viniferin, E-e-viniferin, caraphenol and d-vini- grapes were similar and maintained at the same level
ferin, and resveratrol trimers, such as a-viniferin and during 5 days of storage. Treatment of undamaged or
E-miyabenol C (Table 2, no. 22) (Flamini et al. 2016). damaged grapes with UV-C and fungal infection
However, infection with this pathogen decreased the increased the content of E-resveratrol 6 times (7 or 4
E-piceid and E-astringin concentrations by approxi- times for undamaged and damaged berries, respec-
mately onefold in grapes. The total stilbene content in tively, in the case of infection and subsequent UV-C
grapes infected with A. carbonarius increased 60% treatment) compared with that in non-inoculated
over that in uninfected berries. Glycosylation of samples, after 5 days of storage. In addition, the E-
stilbenes is involved in storage, transport from the resveratrol content did not increase in damaged grapes
cytoplasm to the apoplasm and protection from compared to that in undamaged grapes either at day 0
peroxidative degradation. Subsequent storage in vac- or after storage. These results suggest that E-resver-
uoles may protect plant cells from potentially their atrol was not elicited by ochratoxigenic Aspergillus.
toxic effects. In susceptible cultivars, such as Gamay, However, in another study, it has been shown that E-
Gamaret, Pinot Noir and Chasselas, resveratrol was resveratrol accumulates in A. carbonarius inoculated
found to be either glycosylated or present in very low grapes at veraison time but not during ripening
concentrations (Pezet et al. 2004). Studies of grape- (Bavaresco et al. 2003). Moreover, E-resveratrol
vine varieties resistant to P. viticola reported that E- formation decreases from veraison to ripening in
resveratrol is probably rapidly oxidized into toxic berries, and this formation is elicited by biotic
stilbenes, such as e- and d-viniferins, while in (ochratoxigenic Aspergillus) and abiotic (UV-C) fac-
susceptible cultivars, it is rapidly glycosylated into tors (Jeandet et al. 1991). However, the induction
less toxic piceid (Pezet et al. 2004). In the case of capacity for E-resveratrol was sevenfold, which is
infected Negro Amaro grape, the content of E- higher than the value of 4.4-fold obtained in another
resveratrol increased, but the content of glycosylated study (Cantos et al. 2002). The accumulation of E-
123
Phytochem Rev
resveratrol was significantly enhanced by increasing stimuli may increase the production of stilbenes in
the storage time and by UV-C treatment, as well as by grapes. Several different elicitors, as inducers of
the combination of both treatments. secondary metabolite stilbenes, were analysed: UV
Inoculation with ochratoxigenic Aspergillus did not irradiation, visible light, ultrasonication, signalling
specifically elicit the accumulation of E-piceid compounds and fungicides. Among them, UV treat-
(Table 2, no. 23). However, there was a significant ment was the most effective method to enhance
increase in the induction of E-piceid during storage, stilbene derivative production. The enhancement of
and this induction was particularly faster in damaged the stilbene content depended on the UV wavelength,
berries than in undamaged grapes. Therefore, E-piceid UV intensity, duration of irradiation and developmen-
induction in UV-C untreated grapes could be a tal stage of the berry fruit. The STS expression
consequence of the total microbial contamination responsible for stilbene production was the highest
rather than a specific response to ochratoxigenic when the berries were exposed to UV-C irradiation at
Aspergillus. On the other hand, in undamaged berries, 2 weeks before veraison. In general, the effect of
the E-piceid content increase in response to microbial biotic stressors, such as Aspergillus carbonarius and
infection was faster in UV-C treated berries than in Botrytis cinerea, was found to be not as efficient as the
untreated berries. The E-piceid content in damaged effect of abiotic elicitors.
grapes after treatment with UV-C and fungal infection
increased onefold. Open Access This article is distributed under the terms of the
Creative Commons Attribution 4.0 International License (http://
UV-C treatment induced the biosynthesis of E- creativecommons.org/licenses/by/4.0/), which permits unre-
resveratrol as a result of an increased transcription of stricted use, distribution, and reproduction in any medium,
genes encoding stilbene synthase. Fungal infection provided you give appropriate credit to the original
induced not only the transcription of stilbene synthase author(s) and the source, provide a link to the Creative Com-
mons license, and indicate if changes were made.
but also the transcription of genes encoding glycosyl-
transferases, which transform E-resveratrol to E-
piceid.
References
123
Phytochem Rev
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