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Project proposal
Submitted to
Seema Mineral & Metals, Udaipur
Submitted by
Directorate of Research
Maharana Pratap University of Agriculture & Technology,
Udaipur
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Project title: Studies on use of Silica in Agriculture
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Experiment No. 1: Studies on effect of soil & foliar applicationsilica on growth, yield &
productivity of maize under organic farming
Treatment details
(A) Soil Application
i) Control (No application)
ii) 50 kg ha-1
iii) 75 kg ha-1
iv) 100 kg ha-1
v) 125 kg ha-1
vi) 150 kg ha-1
(B) Foliar application
i) Control (No spray)
ii) Water spray
iii) 0.25%
iv) 0.50%
v) 0.75%
vi) 1%
Other experiment details
a) Replications : 03
c) Experimental design : Split plot design (main plot- soil applied silicon
f) Plant geometry : 60 cm X 25 cm
g) Variety : PM - 9
Growth attributes
Yield attributes and Yield
Protein content (%)
Nutrient content and uptake
Economic evaluation
Net return (Rs./ha)
B:C ratio
Period: 2 years
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Experiment No. 2: Evaluation of bio-efficacy of silica diatomaceous against
anthracnose of chilli
Objectives:
To study silica diatomaceous and botanical for suppression of Anthracnose Caused by
Colletotrichum capsici in vitro at different concentrations.
To find out silica diatomaceous, and botanical for suppression of the anthracnose of
chilli in organic pot culture.
Plan of work
In vitro studies (Bioassay) of silica diatomaceous at 2%, 4%, 6% and 8% and that of
botanical Neem oil (0.5 %) using food poison technique. Treatments: 6 (including
control) silica diatomaceous with 4 concentrations and botanical with 1 (0.5%)
concentration Replications:5; Design: CRD
Observations
Fungal growth measurement (mm) in diameter
Effect of silica diatomaceous and botanical will be evaluated for suppression
Anthracnose Caused by Colletotrichum capsici in pot culture.
Treatment details
1. Silica diatomaceous 2 %
2. Silica diatomaceous 4 %
3. Silica diatomaceous 6 %
4. Silica diatomaceous 8 %
5. Neem oil (0.5 %)
6. Control
Treatments- 6, Replication- 3, Design- CRD , Spray applications : 2
**Observations: Observations for disease will be recorded on (0-5 disease rating score) and there
by PDI will be calculated
Period: 2 years
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Experiment No. 3: Evaluation of bio-efficacy of silica diatomaceous against alternaria leaf
spot of brinjal
Objectives:
To evaluate silica diatomaceous and botanical for suppression of leaf spot Caused by
Alternaria sp. of brinjal in vitro at different concentrations.
To assess silica diatomaceous and botanical for suppression of the Alternaria leaf spot
of brinjal in organic pot culture.
Plan of work:
In vitro studies (Bioassay) of silica diatomaceous at 2%, 4%, 6% and 8% and that of
botanical Neem oil (0.5 %) using food poison technique. Treatments: 6 (including
control) silica diatomaceous with 4 concentrations and botanical with 1 concentration
(0.5%).
Observations:
Fungal growth measurement (mm) in diameter
Effect of silica diatomaceous and botanical will be evaluated for suppression leaf spot
Caused by Alternaria sp. of brinjal in pot culture.
Treatment details:
1. Silica diatomaceous 2 %
2. Silica diatomaceous 4 %
3. Silica diatomaceous 6 %
4. Silica diatomaceous 8 %
5. Neem oil (0.5 %)
6. Control
Treatments- 6, Replication- 3, Design- CRD, spray applications: 2
**Observations: Observations for disease will be recorded on (0-5 disease rating score)
and there by PDI will be calculated
Period: 2 years
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Experiment No. 4: Evaluation of bio-efficacy of silica diatomaceous earth against rice
weevil in stored maize
Objectives
To evaluate the bio-efficacy of silica diatomaceous, insecticides and botanicals for the
management of rice weevil infesting stored maize.
Plan of work
The experiment on evaluation the effect of diatomaceous earth on Rice weevil infesting
stored maize will be conducted under laboratory conditions. The experiment will be laid out in
CRD with 8 treatments and 3 replications. The 200 g grains / kernels will be treated with
diatomaceous earth and botanicals, stored in the glass jar and the known number of rice weevil
will be released in the stored grains and will be kept in laboratory for the 4 to 12 weeks.
Observations
The observations will be recorded after 4 weeks of the infestation at weekly interval on the basis
of following parameters:
The mortality of rice weevil
Adult emergence
Percent loss in stored grain weight
Treatment Details
1. Silica diatomaceous 1%
2. Silica diatomaceous 2%
3. Silica diatomaceous 3%
4. Silica diatomaceous 4%
5. Neem oil 1 %
6. Nirgudi oil 1%
7. Standard insecticides (at recommended dose)
8. Control
Period: 2 years
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Experiment No. 5: Evaluation of bio-efficacy of silica diatomaceous earth against pulse
beetle in stored soybean
Objectives
To evaluate the bio-efficacy of silica diatomaceous, insecticides and botanicals for the
management of pulse beetle in stored soybean.
Plan of work
The experiment on evaluation the effect of diatomaceous earth on pulse beetle infesting
stored soybean will be conducted under laboratory conditions. The experiment will be laid out in
CRD with 8 treatments and 3 replications. The 200 g grains / kernels will be treated with
diatomaceous earth and botanicals, stored in the glass jar and the known number of pulse beetle
will be released in the stored grains and will be kept in laboratory for the 4 to 12 weeks.
Observations
The observations will be recorded after 4 weeks of the infestation at weekly interval on the basis
of following parameters:
The mortality of pulse beetle
Adult emergence
Percent loss in stored grain weight
Treatment Details
1. Silica diatomaceous 1%
2. Silica diatomaceous 2%
3. Silica diatomaceous 3%
4. Silica diatomaceous 4%
5. Standard insecticides (at recommended dose)
6. Neem oil 1 %
7. Mustard oil 1%
8. Control
Period: 2 years
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Experiment No. 6: Evaluation of bio-efficacy of silica diatomaceous earth against pulse
beetle in stored black gram
Objectives
To evaluate the bio-efficacy of silica diatomaceous, insecticides and botanicals for the
management of pulse beetle in stored black gram.
Plan of work
The experiment on evaluation the effect of diatomaceous earth on pulse beetle infesting
stored black gram will be conducted under laboratory conditions. The experiment will be laid out
in CRD with 8 treatments and 3 replications. The 200 g grains / kernels will be treated with
diatomaceous earth and botanicals, stored in the glass jar and the known number of pulse beetle
will be released in the stored grains and will be kept in laboratory for the 4 to 12 weeks.
Observations
The observations will be recorded after 4 weeks of the infestation at weekly interval on the basis
of following parameters:
The mortality of pulse beetle
Adult emergence
Percent loss in stored grain weight
Treatment Details:
1.Silica diatomaceous 1%
2.Silica diatomaceous 2%
3.Silica diatomaceous 3%
4. Silica diatomaceous 4%
5.Neem oil 1 %
6. Mustard oil 1%
7. Standard insecticides (at recommended dose)
8. 8.Control
Period: 2 years
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Experiment No. 7: Evaluation of bio-efficacy of silica diatomaceous earth against red Flour
beetle in stored wheat
Objectives
To evaluate the bio-efficacy of silica diatomaceous, insecticides and botanicals for the
management of Red flour beetle infesting stored wheat.
Plan of work
The experiment on evaluation the effect of diatomaceous earth on Red flour beetle
infesting stored wheat will be conducted under laboratory conditions. The experiment will be
laid out in CRD with 8 treatments and 3 replications. The 200 g grains / kernels will be treated
with diatomaceous earth and botanicals, stored in the glass jar and the known number of Red
flour beetle will be released in the stored grains and will be kept in laboratory for the 4 to 12
weeks.
Observations
The observations will be recorded after 4 weeks of the infestation at weekly interval on the basis
of following parameters:
The mortality of Red flour beetle
Adult emergence
Percent loss in stored grain weight
Treatment Details
1.Silica diatomaceous 1%
2.Silica diatomaceous 2%
3. Silica diatomaceous 3%
4.Silica diatomaceous 4%
5.Standard insecticides (at recommended dose)
6.Dry Neem Leaves 1 %
7.Nirgudi oil 1%
8.Control
Period: 2 years
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Experiment No.8: Effect of silica enriched farm waste compost on soil fertility
and yield of Pea
Objectives:
To study the effect of silica enriched farm waste compost on yield attributes and
yield of Pea
To study the effect of silica enriched farm waste compost on soil fertility
Treatment details
(i) Control
(ii) Farm waste compost + 1 % silica on dry weight basis
(iii) Farm waste compost + 2 % silica on dry weight basis
(iv) Farm waste compost + 3 % silica on dry weight basis
(v) Farm waste compost + 4 % silica on dry weight basis
(vi) Farm waste compost + 5 % silica on dry weight basis
Other experiment details
a) Replications : 4
b) Total plots : 24
c) Experimental design : CRD
Observations
Yield attributes and Yield
Nutrient content and uptake
Soil analysis (Before sowing and after crop harvest N, P, K, Zn, Fe & Si)
Organic carbon
pH& EC
Period: 2 years
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Experiment No.9: Effect of silica enriched vermicompost on soil fertility and yield of Pea
Objectives:
Treatment details
(i) Control
(ii) Vermicompost + 1 % silica on dry weight basis
(iii) Vermicompost + 2 % silica on dry weight basis
(iv) Vermicompost + 3 % silica on dry weight basis
(v) Vermicompost + 4 % silica on dry weight basis
(vi) Vermicompost + 5 % silica on dry weight basis
Other experiment details
a) Replications : 4
b) Total plots : 24
Observations
Yield attributes and Yield
Nutrient content and uptake
Soil analysis (Before sowing and after crop harvest N, P, K, Zn, Fe & Si)
Organic carbon
pH& EC
Period: 2 years
Experiment No. 10: Effect of silica application on growth, yield and quality of tomato.
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Objectives:
To study the effect of silica (Soil Application) on growth and yield of Tomato.
To study the effect of silica (Soil Application) on quality of Tomato.
Treatment details
i. Control
ii. 25 tonnes FYM / ha
iii. 20 Kg / ha Silica Diatomaceous
iv. 25 tonnes FYM / ha + 40 Kg / ha Silica Diatomaceous
v. RDF + 25 Tonnes FYM / ha + 60 Kg / ha Silica Diatomaceous
vi. RDF + 25 Tonnes FYM/ ha + 80 Kg / ha Silica Diatomaceous
vii. RDF + 25 Tonnes FYM / ha + 100 Kg / ha Silica Diatomaceous
Replications: 3
Design: CRD
Observation:
i. Plant height at 60 And 90 DAT (cm)
ii. Chlorophyll content
iii. No. of fruits per plant
iv. Weight of fruits per plant (g)
v. Vitamin C content of fruit (mg/100g)
vi. Dry matter content (%)
vii. Net returns
viii. B:C ratio
Period: 2 years
Experiment No. 11: Effect of foliar application of silica on growth, yield and quality of tomato
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Objectives:
To study the effect of silica (Foliar Application) on growth and yield of Tomato.
To study the effect of silica (Foliar Application) on quality of Tomato.
Treatment details
i. Control
ii. 25 Tonnes FYM / ha + Silica Diatomaceous 2 %
iii. RDF+ Silica Diatomaceous 2 %
iv. RDF+ 25 Tonnes FYM / ha + Silica Diatomaceous 2 %
v. RDF+ 25 Tonnes FYM / ha + Silica Diatomaceous 4 %
vi. RDF+ 25 Tonnes FYM / ha + Silica Diatomaceous 6 %
vii. RDF+ 25 Tonnes FYM / ha + Silica Diatomaceous 8 %
Replications: 3
Design: CRD
Observation
i. Plant height at 60 And 90 DAT (cm)
ii. Chlorophyll content
iii. No. of fruits per plant
iv. Weight of fruits per plant (g)
v. Vitamin C content of fruit (mg/100g)
vi. Dry matter content (%)
vii. Net returns
viii. B:C ratio
Period: 2 years
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Experiment No.12: Bio-efficacy of Silica diatomaceous earth for the management of root-
knot nematode, Meloidogyne incognita in tomato.
Objective : To find out the perfect dose of Silica diatomaceous earth against root-knot
nematode, M. incognita in tomato.
Plan of work :Experiments on evaluating the effect of Silica diatomaceous earth on root knot
nematode, M. incognita infecting tomato will be conducted under pot condition in cage house.
The experiment will be laid out in CRD with 8 treatments each with 3 replications. The 9’’
earthen will be filled with the pre-existing root-knot nematode infested soil over economic
threshold level and the respective treatments will provided in each pot 15 days prior to
transplanting of tomato seedlings.
Two sets of experiment will be conducted, a) nursery treatment with silica diatomaceous earth @
4% w/w along with vermicompost and
b) untreated nursery
The seedlings raised in both the nurseries will be transplanted separately for further studies, viz.
Treatments :
i. Soil Application of Silica diatomaceous earth (SDE)@ 2%w/w
ii. Soil Application of Silica diatomaceous earth (SDE)@ 4%w/w
iii. Soil Application of Silica diatomaceous earth (SDE)@ 6%w/w
iv. Soil Application of Silica diatomaceous earth (SDE)@ 8%w/w
v. Soil Application of Silica diatomaceous earth (SDE)@ 10%w/w
vi. Soil Application of Trichoderma viride @ 2% w/w
vii. Soil Application of Carbofuran 3G @ 35 mg/Kg soil
viii. Untrated control
After 45 days of transplanting, the plants will be uprooted and desired observations will
be recorded.
Observations:
A. Host-Plant Parameters
i. Shoot length
ii. Fresh Shoot weight
iii. Root length
iv. Fresh Root weight
B. Nematode Parameters
i. Initial nematode population per 200 cc soil
ii. Final nematode population per 200 cc soil
iii. No of galls/knots per plant
iv. No of eggmasses per plant
v. No of eggs & larvae per egg mass
vi. Total nematode population.
Period: 2 years
Name: Dr. M.K. Sharma Designation: Prof. & Head (PI) Signature
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Experiment No.13: Bio-efficacy of Silica diatomaceous earth for the management of
reniform nematode, Rotylenculus reniformis in Capsicum
Objective: To find out the perfect dose of Silica diatomaceous earth against reniform
nematode, Rotylenculus reniformis in Capsicum.
Plan of work: An experiment on evaluating the effect of Silica diatomaceous earth on root knot
nematode, M. incognita infecting Capsicum seedling will be planted under pot condition in cage
house. The experiment will be laid out in CRD with 8 treatments each with 3 replications.
The 9’’ earthen will be filled with the pre-existing root-knot nematode infested soil over
economic threshold level and the respective treatments will provided in each pot 15 days prior to
sowing of mung.
Treatments:
i. Soil Application of Silica diatomaceous earth (SDE)@ 2%w/w
ii. Soil Application of Silica diatomaceous earth (SDE)@ 4%w/w
iii. Soil Application of Silica diatomaceous earth (SDE)@ 6%w/w
iv. Soil Application of Silica diatomaceous earth (SDE)@ 8%w/w
v. Soil Application of Silica diatomaceous earth (SDE)@ 10%w/w
vi. Soil Application of Trichoderma viride @ 2% w/w
vii. Soil Application of Carbofuran 3G @ 35 mg/Kg soil
viii. Untreated control
After 45 days of sowing, the plants will be uprooted and desired observations will be
recorded.
Observations:
C. Host-Plant Parameters
i. Shoot length
ii. Fresh Shoot weight
iii. Root length
iv. Fresh Root weight
D. Nematode Parameters
i. Initial nematode population per 200 cc soil
ii. Final nematode population per 200 cc soil
iii. No of galls/knots per plant
iv. No of eggmasses per plant
v. No of eggs & larvae per egg mass
vi. Total nematode population.
Period: 2 years
Name: 1. Dr. K.D. Ameta (PI) Assistant Professor, (Horticulture), Signature
RCA, Udaipur
2. Dr. M.K. Sharma (Co-PI) Designation: Prof. & Head Signature
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Experiment No. 14: Effect of Silicon on Growth, Yield and Quality of Organic Wheat
(Triticum aestivum L.)
Objectives
To study the effect of silicon on growth and yield of organic wheat.
Treatment details
A. Doses of silicon
D1 : Control (water spray only)
D2: 2 g/liter water
D3 : 4 g/liter water
D4 : 6 g/liter water
D5 : 8 g/liter water
B. Stage of silicon application
S1 : CRI stage
S2 : Jointing stage
S3 : Tillering stage
Experimental details
b) Replications : 03
c) Total plots : 45
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by SGS. The product will be procured from Seema
Minerals & Metals, Udaipur. The diatomonas earth
contains 80.69 % SiO2.
Observations:
Growth attributes
Yield attributes and Yield
Nutrient content and uptake
Economic:
o Net return (Rs./ha)
o B:C ratio
Period: 2 years
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Budget
S. No. Particulars Year Total
I II
1 Contractual Services 2.40 2.40 4.80
Two skilled persons @ Rs. 10,000/month
2 Recurring Contingency (inputs, chemicals, lab 11.00 11.00 22.00
expenditure, labour analysis fee, data recording
system web portal & on-line information availability,
data card ITK data workshop, training etc.)
3 Non Recurring Contingency 0.50 0.50 0.50
Total 13.90 13.90 27.80
Investigators:
(a) Principal Investigator:
Dr. S.K. Sharma, Professor (Agronomy) & Zonal Director Research, MPUAT, Udaipur
(b) Co-Principal Investigators:
1. Dr. Roshan Choudhary, Assistant Professor, Agronomy, RCA, Udaipur
2. Dr. Gajanand Jat, Assistant Professor, Soil Science, RCA, Udaipur
3. Dr. Amit Trivedi, Associate Professor, Plant Pathology, RCA, Udaipur
4. Dr. Hemant Swami, Assistant Professor, Entomology, RCA, Udaipur
5. Dr. B. G. Chhipa, Assistant Professor, Horticulture, RCA, Udaipur
6. Dr. R.S. Choudhary, Assistant Professor, Agronomy, RCA, Udaipur
7. Dr. M.K. Sharma, Prof. & Head, Nematology, RCA, Udaipur
8. Dr. K.D. Ameta, Assistant Professor, Horticulture, RCA, Udaipur
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CERTIFICATE OF UNDERTAKING
PI Co-PI Co-PI
Dr. S. K. Sharma Dr. Roshan Choudhary Dr. Amit Trivedi Associate
Zonal Director Research Assistant Professor, Professor, Plant Pathology,
ARS, Udaipur Agronomy, RCA, Udaipur RCA, Udaipur
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Director Research
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