NAME: Latiyah Timothy

LAB DAY: Friday

NAME OF PARTNER: Annesha Sylvester, Ranelle Stewart, Mitra Suchit, Anjanie Sharma and
Siddiiqa Seunath
DEMONSTRATOR: Charran
DATE: Friday 1st November 2019
EXPERIMENT: # 8: Kinetics of the Decolourization of an Organic Dye (Crystal Violet) in Basic
Media
OBJECTIVES:

 To prepare standard solutions of varying concentrations of organic dye and construct a

calibration curve to determine the concentration of dye during the reaction.
 To follow the rate of decolourization of an organic dye in basic media by

RESULTS:
Table 1: Standards Solutions Table

Flask Required Actual Concentration ur Corrected

Volume volume of of CV (M) Absorbance
Of Stock CV Stock CV
(ml) (ml)
A 50 50 3.84 x10-5 1.132 1.076
B 40 50 3.07 x10-5 0.926 0.870
C 30 50 2.30 x10-5 0.714 0.658
D 20 50 1.54 x10-5 0.498 0.442
E 10 50 7.68 x10-6 0.313 0.257
F 5 50h 3.84 x10-6 0.212 0.156
DI H2O 0 0 0.00 0.056 0.000
Table 2: Absorbances and the Determined [CV] Obtained for Experiment B for 3.84 x 10 -5 M

Time (s) Absorbance Concentration In [CV] 1/[CV]

of Crystal Violet
(M)
Initial 0.395 1.34 x10-5 -11.22 7.47 x104
60 0.130 3.69 x10-6 -12.51 2.71 x105
80 0.118 3.25 x10-6 -12.64 3.08 x105
100 0.107 2.85 x10-6 -12.77 3.51 x105
120 0.100 2.59 x10-6 -12.86 3.86 x105
140 0.093 2.34 x10-6 -12.97 4.28 x105
160 0.088 2.15 x10-6 -13.05 4.64 x105
180 0.085 2.04 x10-6 -13.10 4.89 x105
200 0.082 1.93 x10-6 -13.16 5.17 x105
220 0.079 1.82 x10-6 -13.21 5.48 x105
240 0.077 1.75 x10-6 -13.26 5.71 x105
260 0.075 1.68 x10-6 -13.30 5.96 x105
280 0.074 1.64 x10-6 -13.32 6.09 x105
300 0.073 1.61 x10-6 -13.34 6.23 x105
320 0.073 1.61 x10-6 -13.34 6.23 x105
340 0.072 1.57 x10-6 -13.37 6.38 x105
360 0.071 1.53 x10-6 -13.39 6.53 x105
380 0.071 1.53 x10-6 -13.39 6.53 x105
400 0.071 1.53 x10-6 -13.39 6.53 x105
420 0.070 1.50 x10-6 -13.41 6.69 x105
440 0.070 1.50 x10-6 -13.41 6.69 x105
460 0.069 1.46 x10-6 -13.44 6.85 x105
480 0.069 1.46 x10-6 -13.44 6.85 x105
500 0.069 1.46 x10-6 -13.44 6.85 x105
TABLE 3: Absorbances and the Determined [CV] Obtained for Experiment B for 3.07 x 10 -5 M

Time (s) Absorbance Concentration In [CV] 1/[CV]

of Crystal Violet
(M)
Initial 0.393 1.33 x10-5 -11.23 7.52 x104
60 0.124 3.47 x10-6 -12.57 2.88 x105
80 0.100 2.59 x10-6 -12.86 3.86 x105
100 0.098 2.52 x10-6 -12.89 3.97 x105
120 0.091 2.26 x10-6 -13.00 4.42 x105
140 0.085 2.04 x10-6 -13.10 4.89 x105
160 0.08 1.86 x10-6 -13.19 5.37 x105
180 0.077 1.75 x10-6 -13.26 5.71 x105
200 0.074 1.64 x10-6 -13.32 6.09 x105
220 0.073 1.61 x10-6 -13.34 6.23 x105
240 0.071 1.53 x10-6 -13.39 6.53 x105
260 0.069 1.46 x10-6 -13.44 6.85 x105
280 0.068 1.42 x10-6 -13.46 7.03 x105
300 0.068 1.42 x10-6 -13.46 7.03 x105
320 0.067 1.39 x10-6 -13.49 7.22 x105
340 0.067 1.39 x10-6 -13.49 7.22 x105
360 0.067 1.39 x10-6 -13.49 7.22 x105
380 0.067 1.39 x10-6 -13.49 7.22 x105
400 0.067 1.39 x10-6 -13.49 7.22 x105
420 0.066 1.35 x10-6 -13.52 7.41 x105
440 0.066 1.35 x10-6 -13.52 7.41 x105
460 0.066 1.35 x10-6 -13.52 7.41 x105
480 0.066 1.35 x10-6 -13.52 7.41 x105
500 0.066 1.35 x10-6 -13.52 7.41 x105
TABLE 4: Absorbances and the Determined [CV] Obtained for Experiment B for 2.30 x 10 -5 M

Time (s) Absorbance Concentration In [CV] 1/[CV]

of Crystal Violet
(M)
Initial 0.239 2.81 x10-6 -12.78 3.56 x105
60 0.106 2.81 x10-6 -12.78 3.56 x105
80 0.096 2.45 x10-6 -12.92 4.09 x105
100 0.089 2.19 x10-6 -13.03 4.57 x105
120 0.084 2.01 x10-6 -13.12 4.98 x105
140 0.08 1.86 x10-6 -13.19 5.37 x105
160 0.077 1.75 x10-6 -13.26 5.71 x105
180 0.075 1.68 x10-6 -13.30 5.96 x105
200 0.074 1.64 x10-6 -13.32 6.09 x105
220 0.072 1.57 x10-6 -13.37 6.38 x105
240 0.071 1.53 x10-6 -13.39 6.53 x105
260 0.071 1.53 x10-6 -13.39 6.53 x105
280 0.070 1.50 x10-6 -13.41 6.69 x105
300 0.069 1.46 x10-6 -13.44 6.85 x105
320 0.068 1.42 x10-6 -13.46 7.03 x105
340 0.068 1.42 x10-6 -13.46 7.03 x105
360 0.067 1.39 x10-6 -13.49 7.22 x105
380 0.066 1.35 x10-6 -13.52 7.41 x105
400 0.066 1.35 x10-6 -13.52 7.41 x105
420 0.064 1.28 x10-6 -13.57 7.84 x105
440 0.064 1.28 x10-6 -13.57 7.84 x105
460 0.064 1.28 x10-6 -13.57 7.84 x105
480 0.064 1.28 x10-6 -13.57 7.84 x105
500 0.064 1.28 x10-6 -13.57 7.84 x105
TABLE 5: Absorbances and the Determined [CV] Obtained for Experiment B for 1.54 x 10 -5 M

Time (s) Absorbance Concentration In [CV] 1/[CV]

of Crystal Violet
(M)
Initial 0.151 4.46 x10-6 -12.32 2.24 x105
60 0.094 2.37 x10-6 -12.95 4.21 x105
80 0.088 2.15 x10-6 -13.05 4.64 x105
100 0.084 2.01 x10-6 -13.12 4.98 x105
120 0.081 1.90 x10-6 -13.17 5.27 x105
140 0.078 1.79 x10-6 -13.23 5.59 x105
160 0.076 1.71 x10-6 -13.28 5.83 x105
180 0.075 1.68 x10-6 -13.30 5.96 x105
200 0.073 1.61 x10-6 -13.34 6.23 x105
220 0.072 1.57 x10-6 -13.37 6.38 x105
240 0.072 1.57 x10-6 -13.37 6.38 x105
260 0.071 1.53 x10-6 -13.39 6.53 x105
280 0.069 1.46 x10-6 -13.44 6.85 x105
300 0.069 1.46 x10-6 -13.44 6.85 x105
320 0.067 1.39 x10-6 -13.49 7.22 x105
340 0.067 1.39 x10-6 -13.49 7.22 x105
360 0.065 1.31 x10-6 -13.54 7.62 x105
380 0.063 1.24 x10-6 -13.60 8.07 x105
400 0.063 1.24 x10-6 -13.60 8.07 x105
420 0.062 1.20 x10-6 -13.63 8.31 x105
440 0.062 1.20 x10-6 -13.63 8.31 x105
460 0.062 1.20 x10-6 -13.63 8.31 x105
480 0.062 1.20 x10-6 -13.63 8.31 x105
500 0.062 1.20 x10-6 -13.63 8.31 x105

Table 6: Absorbances Obtained for the Reaction at 278 K (5 0C)

Time (s) Absorbance Concentration of In [CV]

Crystal Violet (M)
0 0.980 3.48 x10-5 -10.27
60 0.898 3.18 x10-5 -10.36
120 0.763 2.68 x10-5 -10.53
180 0.734 2.58 x10-5 -10.57
240 0.684 2.39 x10-5 -10.64
300 0.680 2.38 x10-5 -10.65
360 0.661 2.31 x10-5 -10.68
420 0.620 2.16 x10-5 -10.74
480 0.540 1.87 x10-5 -10.89
540 0.621 2.16 x10-5 -10.74
600 0.445 1.52 x10-5 -11.09
Table 7 298K

Time (s) Absorbance Concentration of In [CV]

Crystal Violet (M)
0 0.515 1.78 x10-5 -10.94
60 0.209 6.58 x10-6 -11.93
120 0.134 3.84 x10-6 -12.47
180 0.101 2.63 x10-6 -12.85
240 0.082 1.93 x10-6 -13.16
300 0.075 1.68 x10-6 -13.30
360 0.070 1.50 x10-6 -13.41
420 0.068 1.42 x10-6 -13.46
480 0.068 1.42 x10-6 -13.46
540 0.067 1.39 x10-6 -13.49
600 0.067 1.39 x10-6 -13.49

Table 8 308 K

Time (s) Absorbance Concentration of In [CV]

Crystal Violet (M)
0 0.288 9.47 x10-6 -11.57
60 0.131 3.73 x10-6 -12.50
120 0.111 2.99 x10-6 -12.72
180 0.097 2.48 x10-6 -12.91
240 0.095 2.41 x10-6 -12.94
300 0.093 2.34 x10-6 -12.97
360 0.093 2.34 x10-6 -12.97
420 0.093 2.34 x10-6 -12.97
480 0.093 2.34 x10-6 -12.97
540 0.093 2.34 x10-6 -12.97
600 0.093 2.34 x10-6 -12.97

Table 9

Temperature (T) (1/T) Rate constant (k) In k

(K) (s-1)
278 0.00360 1.31 x10-2 -4.34
298 0.00336 4.29 x10-2 -3.15
308 0.00325 1.79 x10-2 -4.02
DATA ANALYSIS:

Graph of Absorbance vs Concentration of Stock Solution (M)

1.2

f(x) = 27348.79 x + 0.03

1 R² = 1

0.8
Absorbance

0.6

0.4

0.2

0
0.00E+00 5.00E-06 1.00E-05 1.50E-05 2.00E-05 2.50E-05 3.00E-05 3.50E-05 4.00E-05 4.50E-05

Concentration of Stock Solution (M)

Scale: X-axis: Y-axis:

 Graph of Absorbance vs Time (s) For 3.84 x10-5 M
0.45

0.4

0.35

0.3
Absorbance

0.25

0.2

0.15 f(x) = − 0 x + 0.17

R² = 0.34
0.1

0.05

0
0 100 200 300 400 500 600

Time (s)