LAB REPORT: GERMINATION

Plant Physiology

November 30th, 2019

Group N° 4
Franz Chandi
Domenica Monteverde
Ghislayne Vaca
Lady Guanoliquin
Marcelo Cadena
Liceth Minaya

INTRODUCTION
Germination is the process of a seed developing into a new plant. The first step to
occurs the germination process is the imbibition of water, necessary to start to
growth of the primary root (as a result of the cell elongation and a increasing in the
number of cells). Another processes involved in germination are the activation
process, and intra-seminal growth which means the embryo protrusion. In this lab
report we will determined the germination percentage. Germination percentage is
an estimate of the viability of a population of seeds by a germination test.

The jasmonates (including jasmonic acid), are plant hormones that control plant
defenses against herbivore attack and pathogen infection, is responsible for several
physiological roles during plant development and in response to biotic and abiotic
stress. In this lab report we will apply a jasmonic acid treatment to germinate radish
seeds and perform tests to determine: germination percentage and germination
rate of seeds, of each treatment, with the purpose to determine the most effective
treatment. We will use the ANOVA software, the Tukey's test, to make multiple
comparisons until reaching results that show us which is the best treatment for
germination.

MATERIALS
To start the experimentation process where we put our seeds to germinate, we
used 12 plastic container, within of the contairter we used paper towel which was
where we put the seeds to germinate, pipetes necessaries for moisten the seeds,
aluminium foil that simulates the non-light conditions and last but not least the 360
Raphanus sativus seeds, and the 240 mL of 300 μM of Jasmonic acid like the
treatment and 240 mL of Distilled Water like control group.

METHODS
1. Prepare the germination containers with three circular piece of paper towel
inside in each one plastic containers.
2. Label each plastic containers to recognize the treatment applied C, W&D, J&L,
J&D (water control light/dark, jasmonic acid light/dark).
3. Add a couple of milliliters of the jasmonic acid and water to each container
correspondingly its treatments. The paper towel inside will get completely
wet but without excess of water or jasmonic acid inside.
4. Put 30 Raphanus sativus seeds on each container
5. Cover the containers from dark treatments (6) with aluminum foil to avoid
light affecting the seeds. The others containers (6) have to be maintained in
the light.
6. Observe the containers in the morning and night for the next 10 days or until

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 all the seeds germinate.
7. Score the Raphanus sativus seeds as: germinated (radicle emerged) or not
germinated (radicle nor emerged) and take notes.
8. The germination rate is determined by ¿ seeds germinated /total per container

RESULTS

Figure 1. Germinated control (water and light) seeds observed in the morning and
night for 7 days.

Figure 2.
Germinated seeds of water and dark treatment observed in the morning and night
for 7 days.

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 Figure 3.
Germinated
seeds of jasmonic acid with light treatment observed in the morning and night for 7
days.

Figure 4. Germinated seeds of jasmonic acid with dark treatment observed in the
morning and night for 7 days.

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Figure 5. The germination rate with each treatment, water and dark (W&D),
Jasmonic acid and Light (J&L) and Jasmonic acid with dark (J&D).

Germinatio
Treatmen
n
t
Percentage

CONTROL 94%

W&D 94%

J&L 66%

J&D 90%

Table 1. Germination percentage in each treatment.

Table 2. Tukey’s pairwise shows the significantly differences in the 2nd day at the
morning of seeds germination.

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Table 3. Tukey’s pairwise shows the significantly differences in the 6th day at the
morning of seeds germination.

DISCUSION
Discuss how the different treatments will affect germination of the seeds and if your

results agree with those in the literature.

CONCLUSION
- Usar semillas organicas porque el color rojo de las semillas son porque tienen
tiamina lo cual les permite germinar en cualquier condicion ambiental
- Los resultados se comparar con el control
- Usar bibliografia

BIBLIOGRAPHY
Huang Huang, Bei Liu, Liangyu Liu, Susheng Song, Jasmonate action in
plant growth and development, Journal of Experimental Botany, Volume 68,
Issue 6, 1 March 2017, Pages 1349–1359, https://doi.org/10.1093/jxb/erw495

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 Creelman, R. A., & Mullet, J. E. (1995). Jasmonic acid distribution and
action in plants: regulation during development and response to biotic and
abiotic stress. Proceedings of the National Academy of Sciences, 92(10),
4114-4119.