Advantages And Disadvantages Of Paper

Chromatography
Some of the Advantages of Paper Chromatography are:
1. Paper Chromatography requires very less quantitative material.
2. Paper Chromatography is Cheaper compared to other chromatography methods.
3. Both unknown inorganic as well as organic compounds can be identified by paper
chromatography method.
4. Paper Chromatography  do not occupy much space.compared to other analytical methods or
equipments.

Some of the Disadvantages of Paper Chromatography are:

1. Large quantity of sample cannot be applied on paper chromatography.
2. In quantitative analysis paper chromatography is not effective.
3. Complex mixture cannot be separated by paper chromatography.
4. Less Accurate compared to HPLC or HPTLC

 Why is it important to use an autoclave in the hydrolysis of protein?

Autoclaving is a good option for sterilizing solutions and heat-resistant equipment.

Autoclaves kill microbes by hydrolysis and coagulation of cellular proteins, using
intense heat from pressurized steam.

What happens when protein is hydrolysed?

With proteins, H+ and OH- from H2O (water) are inserted into the peptide bond
between amino acids, producing protein fragments called polypeptides of varying sizes.
With prolonged hydrolysis, you would end up with a solution of the amino acids that
were in the protein.

What are the products of hydrolysis of proteins?

Ultimate products of hydrolysis of protein is amino acid. Proteins are polymers made up
of several amino acids. These amino acids are linked through peptide bonds. On
hydrolysis, peptide bonds are broken and amino acids are released.