Name: Alifia Azzahra/22010119130127/C

Exercise 2: Skeletal Muscle Physiology: Activity 4: Tetanus in Isolated Skeletal Muscle Lab Report

Pre-lab Quiz Results

You scored 66% by answering 2 out of 3 questions correctly.

1. Stimulus frequency refers to

You correctly answered: b. the rate that stimulating voltage pulses are applied to an isolated whole skeletal muscle.

2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
Your answer : b. The muscle tension that develops is greater during a state of unfused tetanus.
Correct answer: d. Muscle tension increases and decreases during a state of unfused tetanus.

3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
You correctly answered: a. maximal tetanic tension.

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Experiment Results
Predict Question:
Predict Question: As the stimulus frequency increases further, what will happen to the muscle tension and twitch
appearance with each successive stimulus? Will there be a limit to this response?
You did not answer this question.

Stop & Think Questions:

What begins to happen at around 80 msec?
You correctly answered: c. unfused tetanus develops

How does the trace at 130 stimuli/sec compare with the trace at 50 stimuli/sec?
You correctly answered: b. Fused tetanus develops at this greater stimulus frequency.

How do the traces with 146–150 stimuli per second compare with the trace at 130 stimuli per second?
You correctly answered: d. Maximal tetanic tension develops with these very high stimulation frequencies.

Experiment Data:

Voltage Length Stimuli/sec Active Force Passive Force Total Force

8.5 75 50 5.12 0.0 5.12
8.5 75 130 5.88 0.0 5.88
8.5 75 140 5.91 0.0 5.91
8.5 75 142 5.92 0.0 5.92
8.5 75 144 5.94 0.0 5.94
8.5 75 146 5.95 0.0 5.95
8.5 75 148 5.95 0.0 5.95
8.5 75 150 5.95 0.0 5.95

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Post-lab Quiz Results
You scored 33% by answering 1 out of 3 questions correctly.

1. The term tetanus refers to

Your answer: d. one simultaneous twitch of all the fibers contained within the muscle.
Correct answer: b. sustained muscle tension due to very frequent stimuli.

2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
Your answer: a. The rate of stimulus delivery (frequency) is greater during a state of unfused tetanus.
Correct answer: d. Muscle tension increases and decreases between different values for an unfused tetanus.

3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
You correctly answered: a. maximal tetanic tension.

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Review Sheet Results
1. Describe how increasing the stimulus frequency affected the force developed by the isolated whole skeletal muscle in
this activity. How well did the results compare with your prediction?
You did not answer this question.

2. Indicate what type of force was developed by the isolated skeletal muscle in this activity at the following stimulus
frequencies: at 50 stimuli/sec, at 140 stimuli/sec, and above 146 stimuli/sec.
You did not answer this question.

3. Beyond what stimulus frequency is there no further increase in the peak force? What is the muscle tension called at this
frequency?
You did not answer this question.

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