2.

The optimal temperature for carrying out blood grouping

reaction is 25ºC ± 5ºC. It is not recommended to carry out
the test at 37ºC.

PROCEDURE
A. Modified Tube Test
1. Prepare 2-5% suspension of RBCs in normal saline.
2. To a small test tube (8 mm x 50 mm) add one drop of
Anti-AB (Monoclonal) test.
Anti-AB 3. To the above tube, add one drop of the red blood cell
suspension and mix well.
(Monoclonal) 4. Centrifuge tube at 1000 rpm for 1 min. otherwise the test
may allowed to stand at a room temperature of 25ºC ±
5ºC for 15-60 minutes.
5. Observe the tubes for evidence of macroscopic as well as
microscopic agglutination.
Cat: 31134B Format: 200 tests
B. Rapid Slide Test
For in vitro diagnostic use only. 1. Prepare approximately 10% suspension of RBCs in
normal saline. Alternatively, oxalated whole blood can be
Monoclonal agglutinating sera for the determination of used.
Human blood groups (ABO) 2. Mark out three areas on a glass slide and label them as A,
B and A, B.
INTRODUCTION 3. Add one drop of corresponding reagents to the above
In 1900, Landsteiner first reported the presence of two areas.
antigens viz. A and B on the surface of human red blood 4. Add one drop of the above cell suspension or oxalated
cells. Based on this discovery, he divided human red blood blood to each of the three labeled areas.
cells into three groups viz. A, Band O. Later in 1902, 5. With separate applicator sticks, mix each cell suspension
Decastello and Sturil recognized the existence of a fourth and reagent mixture well.
group viz. AB. Monoclonal IgM agglutinating sera are 6. Tilt the slide back and forth and observe the macroscopic
produced by murine hybridoma cell lines grown in tissue or microscopic agglutination. Tests that fall to show
culture. Each hybridoma produces a single antibody with agglutination within two minutes are considered negative.
well defined characteristics and these antibodies are used
either singly or blended together with other monoclonal Note: In both modified tube and rapid slide tests, it is
antibodies to produce potent and useful reagents1. Anti-AB advisable to include samples of known A, B and O cells as
(Monoclonal) test (Code No. 31134B) is a blend of controls with every batch of tests. Observe the control before
monoclonal IgM antibodies directed against human red blood reading the test samples.
cell antigens A and B.
CAUTION
PRINCIPLE Do not interpret peripheral drying or fibrin strands as
Human red blood cells (RBCs) possessing A or B or both A agglutination.
and B antigens will agglutinate in the presence of antibody
directed towards the respective antigen. Agglutination of red INTERPRETATION
blood cells with Anti-AB (Monoclonal) is a positive test 1. Agglutination of red blood cells by the reagent indicates a
result and indicates the presence of the corresponding Positive test result.
antigen. Anti-AB (Monoclonal) test is especially useful in 2. Absence of agglutination of red blood cells indicates a
detecting the weak variants of A and B which are Negative test result.
occasionally missed by the routinely used monoclonal Anti- 3. Possible reactions with Anti-A (Monoclonal), Anti-B
A and Anti-B Sera2. (Monoclonal) and Anti-AB (Monoclonal) test and their
interpretation are shown in the following table.
SAMPLE
Blood collected with or without anticoagulant may be used. Reaction with Reaction with Reaction with Blood
Carry out the tests as soon as possible after collection. Store Anti-A Anti-B Anti-AB group
the samples at 2-8ºC in case of any delay. (Monoclonal) (Monoclonal) (Monoclonal)
Blood obtained by finger puncture may be tested directly by + - + A
the slide method, but to avoid clotting, blood collected in this - + + B
manner should be mixed with the reagent quickly. + + + AB
- - - O
STORAGE AND STABILITY OF REAGENTS
Anti-AB (Monoclonal) test is stable at 2-8ºC till the expiry
date mentioned on the individual label.

PRECAUTIONS
1. Although the reagent contains a preservative, care should
be taken to avoid extraneous microbial contamination.

31134B/E3/SP 1
PRESENTATION
Item Pack Code
size No.
Anti-AB (Monoclonal) 10 ml 31134B

REFERENCES
1. Messeter L., (1989) Monoclonal antibodies against
ABOantigens.Biotest Bull.4:9-14.
2. Technical Manual of the American Association of Blood
Banks. 10th Ed. (1990) pp. 1SO-187.
3. Lewis S.M., BraIn B.J. and Bates I.: Practical
Haematology, 9th Ed. (2001) pp. 473. Publ: Churchill
Uvlngston. London, U.K.
4. Molliso P.L., Engelfriet C.P. and Contreras M.: Blood
Transfusion in Clinical Medicine, 9th Ed. (1993) pp. 359.
Publ: Backwell Scientific Publication, London, U.K.

Symbols Legend

Bottle containing 10 ml
Anti-AB (Monoclonal)
Consult Instructions for Use
Caution,consult accompanying
documents
IVD In Vitro Diagnostic Medical Device
Temperature limitation

Σ Contains sufficient for n tests

n

Manufacturer

REF Catalogue number

LOT Batch code

Use by

Orgenics Ltd., part of the Inverness

Medical Innovations Group.
P.O.B 360, Yavne 70650, Israel
Tel.: +972-8-942 9201
Fax.: +972-8-943 8758
Version: 31134B/E3/SP
(03/2007)

31134B/E3/SP 2