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Bioactive Peptides in Milk: Nashat Dahiyat
Bioactive Peptides in Milk: Nashat Dahiyat
net
Review
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Abstract
The role of proteins as physiologically active components in the diet is being increasingly
acknowledged. Many of the proteins that occur naturally in raw food materials exert their physiological action
either directly or upon enzymatic hydrolysis in vitro or in vivo. In recent years it has been recognized that
dietary proteins provide a rich source of biologically active peptides. Such peptides are inactive within the
sequence of the parent protein and can be released in three ways: (a) through hydrolysis by digestive
enzymes, (b) through hydrolysis by proteolytic microorganisms and (c) through the action of proteolytic enzymes
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Contents
increasingly acknowledged. Many of the proteins that occur naturally in raw food
materials exert their physiological action either directly or upon enzymatic hydrolysis
in vitro or in vivo. In recent years it has been recognized that dietary proteins provide
a rich source of biologically active peptides. Such peptides are inactive within the
sequence of the parent protein and can be released in three ways: (a) through
microorganisms and (c) through the action of proteolytic enzymes derived from
microorganisms or plants.
Bioactive peptides have been defined as specific protein fragments that have a
positive impact on body functions or conditions and may ultimately influence health
(Kitts & Weiler, 2003). Upon oral administration, bioactive peptides, may affect the
major body systems namely, the cardiovascular, digestive, immune and nervous
systems (Fig. 1)—depending on their amino acid sequence. For this reason, the
the risk of chronic diseases or boosting natural immune protection has aroused a lot of
scientific interest over the past few years. These beneficial health effects may be
the following ways: (a) enzymatic hydrolysis by digestive enzymes, (b) fermentation
of milk with proteolytic starter cultures, (c) proteolysis by enzymes derived from
microorganisms or plants. In many studies, combination of (a) and (b) or (a) and (c),
has proven effective in generation of short functional peptides (Korhonen & Pihlanto,
2003b). Examples of bioactive peptides produced by the above treatments are given
below.
hydrolysis of whole protein molecules. Many of the known bioactive peptides have
enzymes from bacterial and fungal sources—have also been utilized to generate
Bioactive peptides can, thus, be generated by the starter and non-starter bacteria used
in the manufacture of fermented dairy products. The proteolytic system of lactic acid
bacteria (LAB), e.g. Lactococcus lactis, Lactobacillus helveticus and Lb. delbrueckii
ssp. bulgaricus, is already well characterized. This system consists of a cell wall-
et al, 1999).
Precursor
Micro-organisms used Peptide sequence Bioactivity
proteina
heart disease, peripheral arterial disease, and stroke. The renin-angiotensin system is
an important regulator of blood pressure. Therefore, drugs that inhibit the renin-
3.4.15.1) or by blocking angiotensin (AT1) receptors, are widely used in the treatment
they inhibit the production of the vasoconstrictor angiotensin II and they inhibit the
beneficial effects in hypertensive patients, for example, in those with cardiac or renal
inhibiting and thus a blood pressure–lowering effect can be derived from milk
proteins.
ca. 80% of the total protein inventory. Until recently, the main physiological role of
casein in the milk system was widely accepted to be a source of amino acids required
casein micelle system has more recently been proven to be the prevention of
physiological property has been proposed for the whole casein system (or its
individual fractions, for that matter), various peptides hidden (or inactive) in the
amino-acid sequence have been the subject of increasingly intense studies. Much
work regarding those peptides, which are known to possess bioactivities, is currently
glass of milk in the evening, and that babies are soothed after breast or bottle
feeding. Recent studies have provided evidence that peptides exist in dairy
products which play an active role in the nervous system; known as opioid peptides,
against invaders are rather complex; diet is known to play an important role therein.
Research concerning the role of functional peptides in this field is quite recent,
but it already seems very promising. The two main activities are the
immunomodulatory one (i.e. stimulation of the immune system) (Table 4) and the
6. conclusion
obtainable from food proteins. There is no limit therefore to the number of peptides
Each of these peptides may present unique structure and biofunctionalities that can
the prospects of
food-derived bioactive peptides will likely fuel in the pharmaceutical industry an
7. Discussion
been most studied for their physiological effects. A few commercial developments
have been launched on the market and this trend is likely to continue alongside with
an exciting scientific and technological challenge, while at the same time offering
supplements and even pharmaceuticals with the purpose of delivering specific health
benefits.
8. References
Biotechnology
proteins. (267-278).