HS300SR Service Manual R1 PDF

HumaStar 300SR
| Service Manual
Cat. No. 169302 - Revision 1

REVISION LIST OF THE MANUAL
REVISION/DATE REVISION DESCRIPTION

1/2018-FEB-09 First revision
SYSTEM VERSION
VERSION DESCRIPTION
1.0.1.43.4 HI application software
2.29D Firmware
COPYRIGHT
Copyright 2018. Human Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of HUMAN in wri-
ting. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
SERVICE UND SUPPORT

CONTENTS
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS 13
1.1 INTRODUCTION 13
1.2 USER WARRANTY 13
1.3 INTENDED USE OF THE INSTRUMENT 13
1.4 GENERAL SAFETY WARNINGS 14
1.5 DISPOSAL MANAGEMENT CONCEPT 14
1.6 BIOHAZARD WARNING 15
1.7 INSTRUMENT DISINFECTION 15
2 TECHNICAL SPECIFICATION 17
2.1 OPERATION 17
2.2 SAMPLES 17
2.3 REAGENTS 17
2.4 PIPETTING 17
2.5 REACTION 18
2.6 SOFTWARE 18
2.7 ELECTROLYTES 18
2.8 ENVIRONMENT 18
3 INSTRUMENT OVERVIEW 19
3.1 GENERAL DESCRIPTION 19
3.2 MAIN CHARACTERISTICS 19
3.3 INSTALLATION AND REMOVAL FROM SERVICE 19
3.3.1 Shipping and delivery 19
3.3.2 Packaging 19
3.3.3 Responsibilities 20
3.3.4 Before setting-up 20
3.3.4.1 Site 20
3.3.4.2 Environment 21
3.3.4.3 Storage 21
3.3.4.4 Unpacking 21
3.3.5 Installation 23
3.3.5.1 Left side 23
3.3.5.2 Right side 23
3.3.5.3 PC connections 24
3.3.5.4 Instrument connections 24
3.3.5.5 Hydraulic connections 24
3.3.6 Moving 26
3.4 MAIN COMPONENTS 26
3.4.1 CPU board 28
3.4.2 Power board 0 28
3.4.5 Level and shock sensor board 30
3.4.6 Power supplies 30
3.4.7 Ground connections 31
3.4.8 Optical lamp 31
3.4.9 Hydraulics panel 32
3.4.10 Hydraulics panel - manifold 35
3.4.11 Wash station 35
3.4.12 External tanks 37
4 SERVICE MENU 39
4.1 ACCESS 39
4.2 TABS MENU 40
4.3 MOUSE USE 41
4.4 OPERATION 42
4.5 SERVICE MENU 43
4.6 HARDWARE TEST MENU (TE-OS-F1) 44
4.6.1 Hardware Test table 45
4.6.2 Logical positions 46
4.6.2.1 Inner plate 46
4.6.2.2 Outer plate 46
4.6.2.3 Filter selection 47
4.6.2.4 Wash station 47
4.6.2.5 Needle 1 rotation 48
4.6.2.6 Sampling needle 1 48
4.6.2.7 Diluter syringe 1 49
4.6.2.8 Needle 2 rotation 50
4.6.2.9 Sampling needle 2 50
4.6.2.10 Diluter syringe 1 51
4.6.3 Pumps and valves tests (TE-OS-F1-F4) 52
4.6.4 Continuous display of inputs (TE-OS-F1-F5) 53
4.7 MECHANICAL CALIBRATIONS MENU (TE-OS-F2) 54
4.7.1 Operations 54
4.7.2 Calibration rows 57
4.7.2.1 Reagent bottles (ARM 1 and ARM 2) 57
4.7.2.2 Diluent bottle (ARM 1 and ARM 2) 57
CONTENTS
4.7.2.3 Sampling needle wash well (ARM 1 and ARM 2) 58

4.7.2.4 Sample barcode (ARM 1) 58
4.7.2.5 Sample tubes and cups (ARM 1 and ARM 2) 58
4.7.2.6 Reading position (ARM 1) 59
4.7.2.7 Wash station needles dispensation (ARM 1 and ARM 2) 59
4.7.2.8 Wash station needles down (ARM 1) 59
4.7.2.9 Reaction rotor (ARM 1 and ARM 2) 60
4.7.2.10 Needle rotation (ARM 1 and ARM 2) 60
4.8 MECHANICAL CHECK MENU (TE-OS-F3) 61
4.8.1 Mechanical checks (TE-OS-F3-F1 to TE-OS-F3-F5) 62
4.8.2 OP reading movement (TE-OS-F3-F6) 63
4.8.3 Diluters check (TE-OS-F3-F7) 63
4.8.4 FS inversion check (TE-OS-F3-F8) 64
4.8.5 OP belt check (TE-OS-F3-F9) 64
4.8.6 OP inversion check (TE-OS-F3-F10) 65
4.9 READING TEST MENU (TE-OS-F4) 66
4.9.1 Read all filters (TE-OS-F4-F4) 67
4.9.2 Autozero (TE-OS-F4-F5-1) 68
4.9.3 Autozero repeatability test (TE-OS-F4-F5-2) 69
4.9.4 Reaction rotor reading offset (TE-OS-F4-F6) 71
4.9.4.1 Fine offset adjustment (TE-OS-F4-F6-1) 71
4.9.4.2 Rough offset adjustment (TE-OS-F4-F6-2) 72
4.9.5 Filter wheel test (TE-OS-F4-F7) 72
4.9.5.1 Test FS home repeatability (TE-OS-F4-F7-1) 72
4.9.5.2 Adjust FS offsets (TE-OS-F4-F7-2) 73
4.9.6 Cuvettes status (TE-OS-F4-F8) 74
4.10 WASHING MENU (TE-OS-F5) 75
4.10.1 Wash position (TE-OS-F5-F1) 76
4.10.2 Execute washes (TE-OS-F5-F2) 76
4.10.3 Fill wash cuvette (TE-OS-F5-F3) 77
4.10.4 Startup (TE-OS-F5-F4) 77
4.10.5 Special pumps tests (TE-OS-F5-F5) 77
4.10.5.1 Test pumps (TE-OS-F5-F5-1) 77
4.10.5.2 Fill hydraulic tubes – the pumps self-test (TE-OS-F5-F5-2) 78
4.10.5.3 Empty tubes (TE-OS-F5-F5-3) 80
4.10.5.4 Check wash station residue (TE-OS-F5-F5-4) 80
4.10.5.5 Adjust pumps (TE-OS-F5-F5-5) 80
4.11 DIAGNOSTICS MENU (TE-OS-F6) 81
4.12 EDIT PARAMETERS MENU (TE-OS-F7) 82
4.12.1 Parameter pages 83
4.12.2 Select parameter (TE-OS-F7-F1) 84
4.12.3 Go to next/previous page (TE-OS-F7-F2/TE-OS-F7-F3) 84
4.12.4 Unlock/lock parameters (TE-OS-F7-F4) 84
4.12.5 Find parameter (TE-OS-F7-F5) 84
4.12.6 Backup parameters (TE-OS-F7-F6) 84
4.12.7 Restore parameters (TE-OS-F7-F7) 85
4.12.8 Screenshots of all parameters (TE-OS-F7-F8) 85
4.12.9 Save the parameters list as a text file 85
4.13 CLEAR ERRORS MENU (TE-OS-F8) 85
5 ADJUSTMENTS 87
5.1 REACTION ROTOR TEMPERATURE 87
5.2 OPTICAL OFFSET AND GAIN 88
5.2.1 Necessary tools 88
5.2.2 Offset adjustment 88
5.2.3 Gain adjustment 89
5.2.4 Reading cell SELECTION SHORTCUT 90
5.2.5 INTERFERENCE FILTERS 90
5.2.6 READINGS OUT OF RANGE 90
5.3 FILTER WHEEL 90
5.3.1 NECESSARY TOOLS 90
5.3.2 OPTICAL HOME SWITCH ADJUSTMENT 91
5.3.3 FS BELT ADJUSTMENT 91
5.3.4 OFFSET ADJUSTMENT 92
5.4 REACTION ROTOR BELT TIGHTENING 92
5.4.1 NECESSARY TOOLS 92
5.4.2 OP BELT TIGHTENING 92
5.4.3 Access to the OP motor screws 93
5.5 SAMPLING NEEDLE VERTICAL ADJUSTMENT IN THE WASH WELL 94
5.6 WASH STATION DOWN ADJUSTMENT 95
5.6.1 ROUGH AUTOMATIC CALIBRATION 95
5.6.2 FINE CALIBRATION 95
6 SERVICING 97
6.1 LAMP REPLACEMENT 97
6.2 SAMPLE TRAY REPLACEMENT (40 / 60 POSITIONS) 99
6.3 SINGLE REACTION CELL REPLACEMENT 102
6.4 REACTION ROTOR REPLACEMENT 103
6.5 SAMPLING NEEDLE REPLACEMENT 104
6.6 FILTER REPLACEMENT AND EQUALIZATION 106
CONTENTS
6.7 VACUUM PUMP REPLACEMENT 107

6.8 VACUUM PUMP MEMBRANE VALVES REPLACEMENT 108
6.9 PERISTALTIC PUMP HEAD REPLACEMENT 109
6.10 DILUTER REPLACEMENT 109
6.11 DILUTER SEALING GASKET REPLACEMENT 111
6.12 SAMPLING ARM REPLACEMENT 112
6.13 LEVEL SENSOR WIRING REPLACEMENT 113
6.14 EXTERNAL TANKS FLOATS REPLACEMENT 118
6.15 INVERSION OF THE FLOAT CONTACT 119
6.16 OPTICAL PREAMPLIFIER REPLACEMENT 119
6.17 ADDING A NEW OPTICAL INTERFERENCE FILTER 121
7 FIRMWARE UPDATING 123

7.1 DOWNLOAD/UPLOAD OF THE SYSTEM PARAMETERS 123
7.1.1 Download 123
7.1.2 Upload 124
7.2 SPECIAL USB CABLE 125
7.3 CPU BOARD VERSION 2.5.1 125
7.4 UPGRADE PROCEDURE 125
7.4.1 EDIF terminal release 2.3.5 126
7.4.2 HumaStar 300SR 126
7.4.3 Upgrade 126
7.4.4 EDIF terminal release 2.3.5 127
8 HI SOFTWARE INSTALLATION 129

8.1 SETTINGS 129
8.2 HI SOFTWARE UPDATING 129
8.2.1 Log on 129
8.2.2 Create a backup copy of the data folder 129
8.2.3 Install the new software 129
8.2.4 Installation steps 130
8.3 HI DATA FOLDER 131
8.4 COMPATIBILITY WITH WINDOWS 7, 8, 8.1, 10 131
8.5 DEVICE DRIVERS 131
9 TROUBLESHOOTING 133
9.1 THE ANALYZER DOES NOT CONNECT 133
9.1.1 Not connected 133
9.1.2 Notes on the USB-to-RS232 adapter 134
9.2 OPTICAL READINGS HAVE BAD REPEATABILITY 134
9.2.1 Noise on the optical signal 135
9.2.2 Imprecision in the reaction rotor positioning 135
9.2.3 Imprecision in the filter wheel positioning 135
9.2.4 Dispensation problems 135
9.2.5 Incorrect vertical positioning of the needle 136
9.3 AUTOZERO HAS BAD REPEATABILITY, UNSTABLE OPTICAL SIGNAL 136
9.4 REACTION ROTOR TEMPERATURE IS UNCONTROLLED 137
9.5 REACTION CUVETTES BECOME DIRTY AND EXCLUDED QUICKLY 138
9.5.1 First checks 138
9.5.2 Check the reaction cuvettes 138
9.5.3 Check the cause of high absorbances 139
9.5.4 Execute the pumps test 139
9.5.5 Execute cuvettes special wash 140
9.5.6 Instrument displays yellow rather than green cuvettes 140
9.6 A DROPLET FORMS ON THE NEEDLE TIP 141
9.6.1 At the end of the needle tip 141
9.6.2 On the side of the needle 142
9.7 ABERRANT OR NULL RESULTS AND REAGENT BOTTLES WRONGLY DE-
TECTED FULL (GREEN) 142
9.8 "NEEDLE SHOCK DETECTOR IS STUCK" ERROR MESSAGE 143
9.9 VACUUM PUMP ERRORS 143
9.9.1 Different causes 143
9.9.2 The TE-OS-F5-F5-1 Test Pumps procedure 144
9.10 NON-REPEATABLE TESTS TROUBLESHOOTING 144
9.10.1 Autozero test 145
9.10.2 Test pumps 145
9.10.3 OP+FS Home 145
9.11 OP CALIBRATION ERROR 145
9.12 INVALID AUTOZERO 146
9.13 SPIKES 146
9.14 WASH STATION DOWN ERROR 147
10 MAINTENANCE 149
10.1 UPDATE FIRMWARE AND SOFTWARE 149
10.2 COVER 149
10.3 OPTICAL LAMP 149
10.4 SAMPLING NEEDLE 150
10.5 WASH STATION NEEDLES 150
10.6 WIRING, CONNECTORS 150
10.7 HYDRAULIC TUBES, FLOATS 151
CONTENTS
10.8 DILUTER 151

10.9 PUMPS 151
10.10 REAGENTS COOLING 151
10.11 SAMPLE PLATE 152
10.12 REACTION ROTOR CUVETTES 152
10.13 TEST AND ADJUSTMENT PROCEDURES IN THE TERMINAL SERVICE
MENU 152
10.13.1 F2 Mechanical calibration 152
10.13.2 F3 Mechanical check 153
10.13.3 F5 Washings menu 153
10.13.4 F4 Reading test menu 153
10.14 DILUTER AND PIPETTING TEST 153
10.14.1 Test procedure for sampling arm 1 154
10.14.2 Test procedure for sampling arm 2 154
11 ASTM LIS INTERFACE 155
12 ERROR CODES 157

12.1 HI SOFTWARE ERROR CODES 157
12.2 FIRMWARE VERSION 2.29D EXECUTION ERROR CODES 160
13 SYSTEM PARAMETERS LIST 163

13.1 IP MOTOR 163
13.2 OP MOTOR 163
13.3 FS MOTOR 164
13.4 SN MOTOR 164
13.5 DS MOTOR 165
13.6 RN MOTOR 166
13.7 WS MOTOR 167
13.8 MOTOR SPEEDS, HOME TOLERANCES 167
13.9 PUMPS 168
13.10 VARIOUS PARAMETERS 170
13.11 BARCODE 171
13.12 LEVEL SENSOR, FLOATS 172
13.13 TEMPERATURES 174
13.14 DILUTIONS, PIPETTING 174
13.15 READINGS 175
13.16 2 ND
ARM SN2 MOTOR 176
13.17 2ND ARM RN2 MOTOR 177
13.18 MISCELLANEOUS 178
Safety InStructIonS 13
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered part of the instrument and must be available to the
operator and the maintenance personnel. For accurate installation, use and
maintenance, please read the following instructions carefully.
In order to avoid damage to the instrument or personal injury, carefully read
the ”GENERAL SAFETY WARNINGS”, describing the appropriate operating pro-
cedures. Please contact your HUMAN authorised local Technical Service in the
event of instrument failure or other difficulties with the instrument.
1.2 User Warranty
HUMAN warrants that instruments sold by one of its authorised

representatives shall be free of any defect in material or workmanship, provided
that this warranty shall apply only to defects which become apparent within
one year from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item within this
warranty period at no charge, except for transportation expenses to the point
of repair.
This warranty excludes the HUMAN representative from liability to replace
any item considered as expendable in the course of normal usage, e.g.: lamps,
valves, syringes, glassware, fuses, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used with
equipment not approved by HUMAN or used for purposes for which it was not
designed.
1.3 Intended Use of the Instrument
The instrument must be used for its intended purpose. It must be ope- [IVD]
rated in perfect technical conditions, by qualified personnel, in such
working conditions and maintained as described in this manual, in the GENERAL
SAFETY WARNINGS. This manual contains instructions for qualified professional
operators.
14
1.4 General Safety Warnings
Use only chemical reagents and accessories specified and supplied by

HUMAN and/or mentioned in this manual. Place the product so that it has
proper ventilation.
The instrument should be installed on a flat, stationary working surface, that is
free of vibrations.
Do not operate in area with excessive dust.
Operate at temperature and at a humidity level in accordance with the
specifications listed in this manual (chapter 2).
Do not operate this instrument with covers and panels removed.
Use only the power cord specified for this product, with the grounding
conductor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this
instrument.
The use of fuses with improper ratings may pose electrical and fire
hazards.
To avoid fire or shock hazard, observe all ratings and markings on the
instrument.
Do not power the instrument in environments that are potentially explosive or
at risk of fire.
Prior to cleaning and/or performing maintenance on the instrument, switch off
the instrument and remove the power cord.
Only cleaning materials described in this manual may be used, as other mate-
rials may damage parts. It is recommended to always wear protective clothing
and eye protection while using this instrument.
All warning symbols that appear in this manual must be carefully observed.
1.5 Disposal Management Concept
The applicable local regulations governing disposal must be observed. It

is the user‘s responsibility to arrange for proper disposal of the individual
components.
All parts which may contain potentially infectious materials must be
disinfected by suitable, validated procedures (autoclaving, chemical treatment)
prior to disposal. Applicable local regulations for disposal must be carefully
observed. The instruments and electronic accessories (without batteries, power
packs etc.) must be disposed of according to the applicable local regulations
for the disposal of electronic components.
HumaStar 300SR | Service manual

Safety InStructIonS 15
Batteries, power packs and similar power sources must be removed from
electric/electronic parts and disposed of in accordance with applicable local
regulations.
1.6 Biohazard Warning
Analytical instruments for in vitro diagnostic application involve the handling

of human samples and controls which should be considered at least potentially
infectious. Therefore every part and accessory of the respective instru-
ment which may have come into contact with such samples must equally
be considered as potentially infectious.
The „BIOHAZARD“ warning label must be affixed to the instrument prior to first
use with biological material!
FIGURE 1-1
Biological Hazard Symbol
1.7 Instrument Disinfection
Before performing any servicing on the instrument it is very important to

thoroughly disinfect all possibly contaminated parts. Before the instrument is
removed from the laboratory for disposal or servicing, it must be
decontaminated. Decontamination must be performed by authorised well-
trained personnel, and in observance of all necessary safety precautions.
16

Technical SpecificaTion 17
2 TECHNICAL SPECIFICATION
2.1 Operation
Clinical chemistry and turbidimetry

Random access, STAT
For HUMAN and Non-HUMAN reagents
3 open channels with HUMAN barcoded reagent bottles
Up to 290 tests/h (without ISE)
2.2 Samples
Removable sample tray

60 positions
12.5 mm primary tubes and 1 ml cups
Optional 16 mm tubes and 3.5 ml cups
Automatic pre- and post-dilution
Internal barcode reader
2.3 Reagents
2 removable reagent trays

42 positions
70 ml and 25 ml bottles
Electronically controlled cooling
2.4 Pipetting
2 pipetting needles
Needle shock detector
Capacitive liquid level detector
Internal, external and method specific wash procedures
18
2.5 Reaction
8-step cuvette wash station

Less than 3 l/h water consumption
2 different wash solutions
Method specific wash procedures
Separate containers for normal and biohazard waste
2.6 Software
External computer required

LIS: Bi-directional, ASTM, ethernet
English, French, Spanish
Designed for touchscreen
Automatic check of reagents, expiry dates, calibration, hardware, mainte-
nance, reaction and results
2.7 Electrolytes
Optional ISE module

Na, K, Cl, Li
2.8 Environment
220-240 or 110-120 V, 50/60 Hz

Less than 300 VA power consumption
93 x 74 x 60 cm (W x D x H)
Approximal 75 kg
16-30 °C, less than 80 % humidity

Instrument overvIew 19
3 INSTRUMENT OVERVIEW
3.1 General description
HumaStar 300SR performs both clinical chemistry and turbidimetric immuno-

chemistry tests. The compact and fully random automated analyzer is especially
suited for medium- and small-size laboratories.
3.2 Main characteristics
Small desktop space, 950 x 780 x 520 mm (width x depth x height). 970 mm
height with open top cover.
Weight approximate 75 kg.
Up to 290 tests/h (without ISE).
Method, needle and cuvette management of incompatible tests.
Easy PAUSE request to add samples and reagents. Automatic recovery from em-
pty reagent bottles. Multiple reagent bottles management.
Extends range of kinetic tests through a dynamic processing of the readings.
Simple access to hydraulic pumps system and optical lamp for easy mainte-
nance.
Run-time sample predilution in reaction well. Automatic standards predilution.
3.3 Installation and removal from service
3.3.1 Shipping and delivery
The new analyzer and its accompanying accessories have been shipped in two
separate containers designed to provide adequate protection during transport
under normal conditions.
3.3.2 Packaging
The analyzer is wrapped in stretch-plastic and securely fixed within a strong

wooden container mounted on a lift-pallet.
The required accessories are placed in a well-packed, heavy cardboard carton.
20
3.3.3 Responsibilities
The shipment of the analyzer generally is the responsibility of the distributor

and delivery is often made in person by an agent of the distributor.
FIGURE 3-1
Indicator TIP-N-TELL (not tipped
or mishandled) Indicator
DROP-N-TELL (impact has not
exceeded 25G)
If the shipment arrives by private or commercial carrier, immediately inspect the

condition and if there is any damage to either containers report it immediately
to both transporter and distributor.
If the damage is extensive, it may be best to refuse the shipment to avoid any
doubt in the attribution of responsabilties.
3.3.4 Before setting-up
Prepare a location for your analyzer based on maximum laboratory efficiency

and adequate work space. Keep in mind the patterns of work and staff circulati-
on to ensure a trouble-free lab operation.
3.3.4.1 Site
The laboratory bench for the analyzer workstation should be a level surface of
solid construction to avoid vibration.
A space of 15 cm on both sides and behind the instrument is the minimum re-
quirement to allow adequate ventilation.
An additional work area on one or both sides of the instrument will contribute
greatly to the work efficiency of the technician during operation.
If sufficient space is available a surface 90 cm deep by 225 cm in working width
will allow a generous surface for the analyzer and the necessities of work (e.g.

tubes, reagents, samples, calibrators, controls, pipettes, user manuals but also
monitor, keyboard, mouse and printer – if necessary).
3.3.4.2 Environment
The location of the analyzer should be dust-free, away from drafts, heat sources
and direct sunlight.
Satisfactory operations may be conducted with temperature ranges from 16 °C
to 30 °C and with a variation during the testing process not to exceed ±2 °C.
Temperatures outside this range may cause erroneous operation.
Air conditioning may be required to ensure result quality if temperatures exceed
these limits.
Relative humidity should not fall below 10 % or rise above 80 % with no conden-
sation.
Ensure that the electromagnetic standards are met. Refer to European directive
on electromagnetic compatibility (see Document 89/336/CEE of 03/05/89).
3.3.4.3 Storage
If for any reason the instrument has been subjected to prolonged storage under
unfavorable conditions a revision by specialized technicians may be required be-
fore proceeding with installation.
3.3.4.4 Unpacking
The analyzer arrives in a wooden container, the top of which is secured with
screws.
The four walls are fixed to the lift-pallet with screws at the bottom line. Taking
off the bottom line screws allow lift up the complete box to get easy access to
the analyzer inside.
FIGURE 3-2
Wood container
! The top cover should be lifted

up by two persons.
22
The analyzer is fixed to the pallet by four mounting brackets. First unscrew the
brackets from the pallet and then loosen the counter nuts to remove the bra-
ckets from the analyzer feet.
FIGURE 3-3
Analyzer fixation
As you have already been told, and as HUMAN suggests, it is best to wait for the
arrival of a representative of the distributor to remove the analyzer from the
container (keep the wooden container in case you need to return the analyzer
for adjustment or repairs).
However, the cardboard carton of miscellaneous items and accessories may be

opened and checked for the following:
- User manual
- USB stick for software installation and HUMAN methods settings
- System solution tank
- Special wash solution tank
- Normal waste tank
- Biologic waste tank
- Tank connections assembly (boxed)
- Accessories kit (boxed)
- Complete "Shipment contents sheet"
FIGURE 3-4
Shipment contents sheet

3.3.5 Installation
After selecting an appropriate site for the instrument and for the PC (inclusive
monitor, keyboard and mouse), you can proceed with the necessary connections.
Connections required are located in two points on the sides of each instrument.
3.3.5.1 Left side
FIGURE 3-5
Left side connections
From top:
- Floats connector (black)
- Liquid sensors connection plug snap-on connectors for:
- System solution tank (blue, not connected)
- Special wash solution tank (green
- Biologic waste tank (yellow)
- Normal waste tank (red)
3.3.5.2 Right side
FIGURE 3-6
Right side switches and connec-
tions
Cooling unit switch (yellow), main power switch (red) with power cord fitting
(incuding fuses compartment) and USB port type B for connection with the PC.
24
3.3.5.3 PC connections
Connect the mouse to a PC port.

Connect the USB touch screen (optional) to a PC port.
Connect the keyboard to a PC port.
Connect the monitor to the PC.
Connect a power cord to the monitor and to an outlet of the power surge pro-
tector.
Connect a power cord to the PC and to an outlet of the online UPS.
FIGURE 3-7
"Schuko" connection
! Adaptors may be required for

local power outlets.
Typically instruments are delivered with "Schuko" connections.
3.3.5.4 Instrument connections
Connect a USB cord to a PC port and to the analyzer port.

Connect a power cord to the instrument and to an outlet of the online UPS.
3.3.5.5 Hydraulic connections
FIGURE 3-8
Vertical positions of the tanks
Place the 4 tanks below the working level.

System solution (blue) and special cleaning solution (green) should be positi-
oned as high as possible under the working level.
Normal waste (red) and biologic waste (yellow) must be positioned as low as
possible.

FIGURE 3-9
Routing of the tubes and the
sensor cables
Route the connecting tubes as straight as possible to avoid the creation of air
bubbles.
The waste tubes must always descend on its way to the waste tanks, in order to
avoid reflux or back pressure.
FIGURE 3-10
Connection of the floats and tubes
assembly to the manifold
! Hydraulic tubes have easy-to-

use snap-on connectors each
with a special valve to prevent li-
quid loss when disconnecting
tubes.
! Ensure that the metal tab of

each connector is released
and ready to be snapped in place
to ensure a safe attachment.
! The presence of bubbles in

the system may be caused by
liquid leakages. Check that the
connectors are correctly snapped
in place.
Insert and rotate the floats bayonet connector (black) to secure the 4 liquid level
sensors.
Connect the 4 tanks to the corresponding hydraulic tube connections of the
analyzer.
26
3.3.6 Moving
The movement of an instrument after it has been put into operation should be
handled with extreme care. One person should never try to move an analyzer
alone. If the instrument needs to be moved over a large distance in or out of the
laboratory please follow the instructions in the sequence below:
- Execute the "Empty entire system" procedure (see User Manual > Mainte-
nance).
- Switch off the analyzer, the computer and the reagent refrigeration, and dis-
connect all lines and tubes connected to the instrument. Be sure to avoid
contact with potentially infectious liquid waste when disconnecting tubes.
- Fix the sampling arm in the uppermost position using the foam protection
tube provided with the shipment.
- At least two persons are now required for moving the analyzer. Lift the in-
strument slowly holding the metal base and in such a way that the cover
remains in the closed position.
- When the analyzer has been located in its new position, reconnect all lines
and tubes according to the installation procedure contained in this manual.
3.4 Main components
FIGURE 3-11
Optical preamplifier board
FIGURE 3-12
Internal USB converter board

FIGURE 3-13
2 level sensor boards
FIGURE 3-14
CPU board
FIGURE 3-15
3 power boards (numbered 0, 1, 2)
28
3.4.1 CPU board
FIGURE 3-16
CPU board details
3.4.2 Power board 0
FIGURE 3-17
Power board 0 details

3.4.3 Power board 1
FIGURE 3-18
3.4.4 Power board 2
FIGURE 3-19
30
3.4.5 Level and shock sensor board
FIGURE 3-20
Level and shock sensor board
details
FIGURE 3-21
Level and shock sensor board
mounted and connected
3.4.6 Power supplies
FIGURE 3-22
Main power and cooling power
supplies
The instrument power supply

- Input 100 – 240 Vac, 50-60 Hz, Output 24Vcc – 500W
-
The refrigerated reagents tray, power supply for cooling
- Input 100 – 240 Vac, 50-60 Hz, Output 15Vcc – 100W
- Output voltage to be adjusted to 13.0 Vcc

3.4.7 Ground connections
FIGURE 3-23
Ground terminal
The ground terminal:

- Ground from the main plug.
- Connection to the mechanical group.
- The two connections to the two metal half-covers are not in use with the
plastic cover.
FIGURE 3-24
Ground connection
The ground connection of the mechanical assembly.
3.4.8 Optical lamp
FIGURE 3-25
Optical lamp
A Lamp fastening knob

B Lamp connector
C Lamp holder
32
The HumaStar has an advanced lamp saving mode that helps to prolong the
lifetime of the halogen lamp.
It is controlled by the parameter (228) LMPSAV. The function is deactivated if
the parameter is set to zero.
If set (the parameter is not zero), after 10 minutes of inactivity and if the instru-
ment is in "Stop" state, the lamp voltage is reduced to 3V. Any user activity will
wake-up the lamp. The lamp returns to full voltage and needs 200 seconds to
be ready for measurement (200 is the recommended setting of the parameter).
3.4.9 Hydraulics panel
FIGURE 3-26
Hydraulics panel
1 Dispensing pump to wash station needle 1

6 Aspirating pump to wash station needle 6
7 Aspirating pump to needle wash well arm 1
8 Dispensing pump to needle wash well arm 1
9 Aspiration pump to needle wash well arm 2
10 Dispensing pump to needle wash well arm 2

FIGURE 3-27
Counter-clockwise peristaltic
pumps operation
FIGURE 3-28
Hydraulics panel rear view (wiring
and tubing)
FIGURE 3-29
Hydraulics panel top view
(electrical connectors)
34
Electrical
Description Device destination
connector
EV1 Valve switching internal (on) / Pinch valve 1
external (off) needle wash 1
EV2 Valve switching internal (on) / Pinch valve 2
external (off) needle wash 2
MDS1 Diluter syringe stepper motor Diluter sampling arm 1
SDS1 Diluter syringe limit switch Limit switch diluter 1
MDS2 Diluter syringe stepper motor Diluter sampling arm 2
SDS2 Diluter syringe limit switch Limit switch diluter 2
PM10 Wash well 2 dispensing pump 10 Lateral nozzle well 2
PM9 Wash well 2 aspiration pump 9 Aspiration well 2
PM8 Wash well 1 dispensing pump 8 Lateral nozzle well 1
PM7 Wash well 1 aspiration pump 7 Aspiration well 1
PM6 Wash station aspiration pump 6 First long needle
PM5 Wash station dispensing pump 5 Short needle 5
FIGURE 3-30
Diluter tubing and pinch valve of
sampling arm 1 and 2
A Input from the needle wash dispensing pump 8/10 (arm 1/2)
B Output to the external needle wash nozzle
C Wash input to the diluter
D Wash output from the diluter to the sampling needle 1/2
E Output to the sampling needle
F Valve switching internal (on) / external (off) needle wash

3.4.10 Hydraulics panel - manifold
FIGURE 3-31
Hydraulics panel manifold
System Dispensing pumps by short needles > pumps 1-3-4-5-8-10

solution
Wash Dispensing pump by second short needle > pump 2
Waste 2 Aspirating pump by just first long needle > pump 6
Waste 1 Aspirating pumps by long needles - pipe cleaner and wash well
waste > pump 7-9 and vacuum 1 - vacuum 2
3.4.11 Wash station
FIGURE 3-32
Wash station top view with
tubing
FIGURE 3-33
Wash station needles assembly
FIGURE 3-34
Wash station top view without
tubing
36
FIGURE 3-35
Wash station bottom view with
tubing (manifold to hydraulics
panel)
FIGURE 3-36
Wash station assembly
FIGURE 3-37 (left)

Wash station wiring
FIGURE 3-38 (right)

Wash station limit switches

3.4.12 External tanks
FIGURE 3-39
External tanks
From left to right:

- 20 l system solution tank (blue)
- 2 l special wash solution tank (green)
- 20 l normal waste 1 tank (red)
- 5 l biologic waste 2 tank (yellow)
FIGURE 3-40
Floats and system solution
connectors - water tube
disconnected (left) / connected
(right)
The floats connector:
A–B Waste 1 float (open = full) Waste 1 tube (red)

C–D Water float (open = empty) Water tube (blue)
E–F Wash float (open = empty) Wash tube (green)
G–H Waste 2 float (open = full) Waste 2 tube (yellow)
The hydraulic tubes have snap-in connectors. The connector can be unplugged
pressing on the lateral metal button.
38

Service menu 39
4 SERVICE MENU
The HI Service Terminal must be seen as a complex, unitary system enabling the
service engineer to perform all configuration, diagnostic, calibration and setup
tasks necessary to the correct functioning of the analyzer.
4.1 Access
To access to the Service Terminal, you must have logged to the HI software with
a "Service" or an "Installer" user name.
Press the "CLOUD" icon button at the center of the bottom part of the screen.
Select then the "TERMINAL" option.
FIGURE 4-1
Access to the Service Terminal
program
By the first window of the Service Terminal you are directly connected to the
analyzer's CPU. All the displayed screens are issued directly by the analyzer and
the PC is just a pass-through terminal.
40
FIGURE 4-2
Start window of the SERVICE
TERMINAL program
4.2 Tabs menu
The tab "Terminal" is the default display.

The tabs "Printer Buffer" and "Settings" are not used for servicing the analyzer.
The tab "Log" allows writing all activities in the Service Terminal to a text file.
Therefor the "Logs" check box has to be enabled, before changing to tab "Termi-
nal" again.
FIGURE 4-3
Logging enabled

Service menu 41
4.3 Mouse use
The mouse has only two functions in the Service Terminal:

- Click inside the light gray middle area of the "Terminal" tab to focus on the
terminal window (a dark gray border line is shown when the window is fo-
cused).
FIGURE 4-4
Terminal window
disabled/enabled (left/right)
- Right click inside the light gray area to select the "Screenshot" or "Print" func-
tions. On a touchscreen monitor these functions can be selected by touching
the enabled area for approximate two seconds.
- Selecting "Screenshot" opens a file selection dialog to define where to store
the screenshot as a ".JPG" file. The filename is automatically given and con-
tains a date and time stamp, e.g. all screenshots are stored in chronological
order.
FIGURE 4-5
Screenshot function selected
- Selecting "Print" allows to print the current screen or to show a preview first.
This function requires an installed printer.
FIGURE 4-6
Print menu selected
42
4.4 Operation
!
board.
Press the CAPS LOCK key to
set the upper case on the key-
To operate the Terminal program, click with the mouse inside the Terminal win-
dow to focus on it.
On a touchscreen monitor just touch inside the light gray area to focus on it.
This is mandatory for the use of
the Service Terminal. There are two function modes:
- "Command" mode, used by the HI software to control the analyzer.
- "Menu" mode, used by the service engineer to operate on the analyzer.
The service engineer can eventually use some of the commands of the "Com-
mand" mode to get some additional information on the instrument status, e. g.
PC<Enter>.
FIGURE 4-7
Result of the
"Menu" command PC

Service menu 43
4.5 Service menu

When starting the Service Terminal, the analyzer is in "Command" mode.
The service engineer enters the "Menu" mode by typing the command OS<Enter>.
FIGURE 4-8
Start screen of the Service Menu
When the service operations are completed, you must exit the "Menu" mode ty-
ping <ESC> several times (depending on the menu level in which you are), until
the screen is cleared.
! The Service menu is control-
led by the function keys F1 to
F10 and by numerical keypad
numbers in case of submenus.
When you are in "Command" mode, typing <Enter> will generate the <NAK> re-
ply to be displayed on the screen. This can be used to verify that you are correctly
again in "Command" mode. ! To address a special function,
a short description will be
used, e.g: TE-OS-F4-F5-2 for the
The calibration, autocalibration and diagnostics system is uniquely powerful. Autozero Test function.
All the needed functions can be easily performed and the faulty components
detected. All the diagnostics can be run in remote control using software like
TeamViewer, allowing the service engineer to remotely acknowledge the pro-
blem and then go on site bringing with him the necessary spare parts.
44
From Service menu you have access to following submenus:
F1 HARDWARE TEST Test all the hardware functions (motors, limit

switches, pumps, readings and temperature).
F2 MECHAN. CALIBR. Calibrate all logical positions.
F3 MECHAN. CHECK Check the motors movements.
F4 READING TEST Test the reading functions.
F5 WASHINGS Test the wash station functions.
F6 DIAGNOSTICS All main diagnostisc and calibraton tests.
F7 EDIT PARAMETERS Edit all system parameters.
F8 CLEAR ERRORS Clear the display of the system errors.
4.6 Hardware Test menu (TE-OS-F1)
FIGURE 4-9
Hardware Test menu
Function keys:
Up "" / down "" Select a motor.

F1 MOTOR HOME Execute the home position of the selected motor.

Service menu 45
F2 MOTOR FWD Move the selected motor forward.

F3 MOTOR BWD Move the selected motor backward.
F4 PUMPS, VALVES Go to the pumps and valves test.
F5 DISPLAY INPUTS Activate the continuous display of the inputs.
F6 HOME ALL MOTORS Execute the home positioning of all motors.
F8 CLEAR ERRORS Clear the display of the not fatal system errors.
M Continuous forward/backward movement for
stress test.
... Go to Edit Parameters pressing "." three times.
4.6.1 HARDWARE TEST TABLE
FIGURE 4-10
Hardware Test table
Colums of the Hardware Test table:
MOT Motor number.

DESCTIPTION Motor description (abbreviation and name).
ENAB Enable/disable the motor current.
0 = current off, FREE
1 = current on, HOLD
SPEED Motor speed in steps/second. This value is read, by default,
from the motors speed system parameter and can be
changed in the hardware test, only for diagnostics, without
affecting the normal work of the analyzer.
IX.STEPS No. of steps that will be executed with the F2 (forward) and
F3 (backward) commands.
LOG.POS. Go to a logical position (Refer to "4.6.2. Logical positions").
ABS.POS. Absolute motors position in steps.
-32768 = motor not initialized.
46
4.6.2 LOGICAL POSITIONS
4.6.2.1 Inner plate
FIGURE 4-11
Inner plate logical positions
SN1 1-60 Select logical position 1 to 60 to move the corresponding

sample tube 1 to 60 under the sampling needle 1.
SN2 101-160 Select logical position 101 to 160 to move the correspon-
ding sample tube 1 to 60 under the sampling needle 2.
4.6.2.2 Outer plate
FIGURE 4-12
Outer plate logical positions
RD 1-80 Select logical position 1 to 80 to move the reaction cell 1

to 80 in the reading position.
WS 101-180 Select logical position 101 to 180 to move the reaction cell
1 to 80 under the wash station needle 1 (rightmost
needle).
SN1 201-280 Select logical position 201 to 280 to move the reaction cell
1 to 80 under the sampling needle 1.
SN2 301-380 Select logical position 301 to 380 to move the reaction cell
1 to 80 under the sampling needle 2.

Service menu 47
4.6.2.3 Filter selection
FIGURE 4-13
Filter selection
Filter n. (0-9) Select 0 to 9 to move the filter 0 to 9 in the reading positi-

on.
4.6.2.4 Wash station
FIGURE 4-14
Wash station logical positions
(111) WSHOME 0/111 Home position (upper optical switch).

(112) WSHIGH 112 Under the home position out of the optical
switch for fast search.
(117) WSTUBE 1 Low immediately over the OP reaction cells for
best calibration checking.
(114) WS_A2D 2 Downwards: Quote for2nd pause during wash
aspiration.
(113) WS_A1D 3 Downwards: Quote for 1st pause during wash
aspiration.
Upwards: Quote for restart aspiration.
(115) WS_ASP 4/115 Needle at the bottom of the reaction cells for
aspiration.
48
4.6.2.5 Needle 1 rotation
FIGURE 4-15
Needle rotation 1 logical
positions
REA 1 - 29 Rotate the needle over the corresponding reagent

bottle.
(086) RN1WSH 86 Wash needle well position.
(092) RN1_OP 92 Reaction rotor dispensing hole.
(090) RN1IP0 90 Outer ring of the samples plate.
(091) RN1IP1 91 Inner ring of the samples plate.
(095) RN1DLC 95 Diluent position.
(085) RN1WRP 85 Wrap position
4.6.2.6 Sampling needle 1
FIGURE 4-16
Needle up/down 1 logical
positions
(044) SN1HIG 44 High, 5 mm over the reagent bottles.

(043) SN1MID 43 5 mm over the 110 mm sample tubes.
(042) SN1LOW 42 5 mm over the reaction cells.
(053) SN1DS2 53 Inside the reaction cell for the second dispensation.
(046) SN1WEL 46 Inside the wash well for internal needle wash.

Service menu 49
(045) SN1WSH 45 At the bottom of the wash well white tip.

(051) SN1PTU 51 At the bottom of the primary sample tubes.
(050) SN1CUP 50 At the bottom of the sample cups inserted on the
sample tray cups adapter.
(054) SN1CU2 54 At the bottom of the sample cups on the inner ring
of the 20+20 samples tray.
(057) SN1DLC 57 At the bottom of the diluent bottle.
(049) SN1REL 49 At the bottom of the large reagent bottles.
(060) SN1RES 60 At the bottom of the small reagent bottles.
55 At the bottom of a tube in the reagent bottles
(055) SN1RET adapter.
(056) SN1REC 56 At the bottom of a cup in the reagent bottles
adapter.
4.6.2.7 Diluter syringe 1
FIGURE 4-17
Diluter syringe 1 volumina
Microliters ±386 The volume of system solution (max. 386 μl) to be

dispensed or aspirated.
50
4.6.2.8 Needle 2 rotation
FIGURE 4-18
positions
REA 1 - 29 Rotate the needle over the corresponding reagent

bottle.
(383) RN2WSH 383 Wash needle well position.
(386) RN2_OP 386 Reaction rotor dispensing hole.
(384) RN2IP0 384 Outer ring of the samples plate.
(385) RN2IP1 385 Inner ring of the samples plate.
(387) RN2DLC 387 Diluent position.
(382) RN2WRP 382 Wrap position
4.6.2.9 Sampling needle 2
FIGURE 4-19
positions
(363) SN2HIG 363 High, 5 mm over the reagent bottles.

(362) SN2MID 362 5 mm over the 110 mm sample tubes.
(361) SN2LOW 361 5 mm over the reaction cells.
(369) SN2DS2 369 Inside the reaction cell for the second dispensation.
(365) SN2WEL 365 Inside the wash well for internal needle wash.

Service menu 51
(364) SN2WSH 364 At the bottom of the wash well white tip.
(368) SN2PTU 368 At the bottom of the primary sample tubes.
(367) SN2CUP 367 At the bottom of the sample cups inserted on the
sample tray cups adapter.
(370) SN2CU2 370 At the bottom of the sample cups on the inner ring
of the 20+20 samples tray.
(373) SN2DLC 373 At the bottom of the diluent bottle.
(366) SN2REL 366 At the bottom of the large reagent bottles.
(374) SN2RES 374 At the bottom of the small reagent bottles.
371 At the bottom of a tube in the reagent bottles
(371) SN2RET adapter.
(372) SN2REC 372 At the bottom of a cup in the reagent bottles
adapter.
4.6.2.10 Diluter syringe 1
FIGURE 4-20
Diluter syringe 2 volumina
Microliters ±386 The volume of system solution (max. 386 μl) to be

dispensed or aspirated.
52
4.6.3 PUMPS AND VALVES TESTS (TE-OS-F1-F4)
! Before testing the pumps, re-

move the wash station need-
les holder and place it in a beaker.
Move the cursor to the pump or valve to be activated and type the number of
hundredths of seconds it must remain activated. This is for testing only and will
not overwrite the set values.
This is to avoid any overflow of
reading cells. The valve that switches the needle wash between the internal wash and the
external wash is parallel to the well aspiration pump. So the well dispensation
pump dispenses internally or externally according to the state of the aspiration
pump.
FIGURE 4-21
Pumps and valves tests
F4 Activates all pumps together.

DSPWH1 Activates the peristaltic pumps 1 to 5 for the dispensation
to needles 1 to 5.
DSPWH5
ASPWH1 Activates the peristaltic pump 6 for the aspiration needle 1.
VACUU1 Activates the vacuum pump 1 for the aspiration needles, except
needle 8.
VACUU2 Activates the vacuum pump 1 for the aspiration needle 8 with
the white tip.
ASPWN1 Activates the wash well 1 aspiration peristaltic pump and
toggles the state of the valve 1.
EV_WN1 Shows the state of the valve 1:
0 = external needle 1 wash
1 = internal needle 1 wash
DSPWN1 Activates the wash well 1 dispensation peristaltic pump for
internal/external needle wash.
ASPWN2 Activates the wash well 2 aspiration peristaltic pump and
toggles the state of the valve 2.

Service menu 53
EV_WN2 Shows the state of the valve 2:

0 = external needle 2 wash
1 = internal needle 2 wash
DSPWN2 Activates the wash well 2 dispensation peristaltic pump for
internal/external needle wash.
4.6.4 CONTINUOUS DISPLAY OF INPUTS (TE-OS-F1-F5)
FIGURE 4-22
Continuous display of inputs
Mot.L.Sw. Indicates the status of the eight home position sensors:

1 = engaged
0 = free
Level Sw. Indicates the status of the level sensors:
1 = no sense
0 = sense
OP temp. Temperature of the OP reaction rotor in tenths of °C.
Reading Instant A/D reading of the optical group.
PWM R= Indicates the voltage rate in % supplying the heating
resistance of the outer plate (0 % to 70 %):
0 % = no heating necessary, 38 °C already reached.
70 % = maximum heating resistance voltage to avoid
overheating.
54
4.7 Mechanical Calibrations menu (TE-OS-F2)
When you select the calibration menu all motors will be homed first for safety.
FIGURE 4-23
Motos homing indication
4.7.1 OPERATIONS
The 39 calibrations are sorted into two pages, named "ARM 1" and "ARM 2":
- Page "ARM 1" contains all 18 calibrations for this arm. It also contains the
barcode reader, reading position and wash needles down calibration.
FIGURE 4-24
Sampling arm 1 calibration menu

Service menu 55
- Page "ARM 2" contains all 18 calibrations for this arm.
FIGURE 4-25
Sampling arm 2 calibration menu
Function keys of the calibration pages:
Page up/Page down Select the previous/next calibration row

F1 CHECK POSITION Return home and check the position.
F2 SAVE POSITION Save the position in the affected system
parameter(s).
F3 ARM1 / ARM 2 Switch between the calibration pages of arm 1
and arm 2.
F4 EDIT PARAMETERS Switches to TE-OS-F8 System Parameters menu
with the cursor at the current parameter.
F5 MOTORS HOME Homes all motors and returns to the current
calibration row.
F6 SPECIAL FUNCT. Allow to check relative movements, levels, water
flow, etc.
Enabled only if the column F6 is not void.
Calling F4 EDIT PARAMETERS, the cursor is
positioned on the parameter listed in the F6
column.
56
UP, DOWN IP/OP MOVE The up "" and down "" keys move the IP or OP
motor step by step.
LEFT, RIGHT RN MOVE The left "" and right "" keys move the RN
motor step by step.
+ - SN/WS MOVE The "+" and "-" keys of the numerical keypad move
the SN or WS motor step by step.
M MOTORS OFF Toggles the motors OFF and ON. When OFF, F5
MOTORS HOME switches the motors ON again.
Every row in the calibrations table represents one particular calibration.
The columns are defined as following:
Column 1 Number of the calibration row.

Column 2 Mnemonic of the calibration row.
Column 3 --IP-- IP parameter, if involved.
Column 4 --OP-- OP parameter, if involved.
Column 5 --RN-- RN parameter, if involved.
Column 6 --SN-- SN parameter, if involved.
Column 7 --WS-- WS parameter, if involved.
Column 8 --F6-- Special function, if applicable. Operated by the F6 key.
The calibration point can be selected by direct address, typing the two digits of
the row. When one parameter of the calibration point is temporary modified, it
is shown in reverse (refer to Figure 4-26, row 01). With the F2 key, the new value
is recorded and the quote value returns to normal .
When a calibration point has been saved with F2, on the far right a v symbol
appears (refer to Figure 4-26, row 02).
Before access to a particular calibration row, the mnemonic names of the invol-
ved parameters are shown (refer to Figure 4-26, rows 03 and 04).
After access to a particular calibration row, the values of the involved parame-
ters are shown (refer to Figure 4-26, rows 01 and 02).
FIGURE 4-26
Marked saved calibrations;
mnemonic or value display

Service menu 57
4.7.2 CALIBRATION ROWS
4.7.2.1 Reagent bottles (ARM 1 and ARM 2)

01 Sampling needle high, 4 mm over the first reagent position, large bottle
type.
F6 checks the liquid level in the large reagent bottle.
02 Sampling needle low at the bottom of the first reagent position, large
bottle type.
Go down step by step, using the "+" key (numerical keypad) until the
vertical collision flag starts moving upwards. Go 7 steps up again, using
the "-" key (numerical keypad). Save the value.
03 Sampling needle high, 4 mm over the last reagent position, small bottle
type.
F6 checks the liquid level in the small reagent bottle.
04 Sampling needle low at the bottom of the last reagent position, small
bottle type.
05 Sampling needle low at the bottom of the first reagent position, cup
type.
21 Sampling needle low at the bottom of the first reagent position, tube
type.
4.7.2.2 Diluent bottle (ARM 1 and ARM 2)
06 Sampling needle high, 4 mm over the diluent position, large bottle type.
F6 checks the liquid level in the large reagent bottle.
07 Sampling needle low at the bottom of the diluent position, large bottle
type.
58
4.7.2.3 Sampling needle wash well (ARM 1 and ARM 2)
08 Sampling needle 5 mm over the wash well for vertical access.

F6 checks the needle position (RN rotation) over the well wash nipple
and the side position for the needle flush.
09 Sampling needle inside the wash well.
There are two versions to calibrate the vertical position of the sampling
needle:
- The best calibration result can be achieved by just touching the bot-
tom of the hole in the white TEFLON nipple by the sampling needle
tip and going 20 steps back.
- Go down with the sampling needle in the hole until the vertical shock
sensor is engaged and go 48 steps back.
F6 checks:
- The sampling needle internal wash inside the white nipple.
- The sampling needle position over the nipple, inside the water drop
(the sampling needle tip must enter inside the drop).
- The external wash of the sampling needle.
- The up movement to check if any droplet remains on the tip of the
sampling needle. (Important for tests, using sample volume less
than 5 μl.)
4.7.2.4 Sample barcode (ARM 1)
10 Sample tray barcode reader calibration for sample position 1:

- The beam must meet the middle of the sample tray handle.
- And the beam must be in the middle between the sample positions
31 and 60 (standard sample tray).
F6 checks the barcode reading for all sample positions (full revolution).
4.7.2.5 Sample tubes and cups (ARM 1 and ARM 2)
11 Sampling needle 5 mm over the sample 1 position, primary tube type.

F6 checks the liquid level in the primary tube.
12 Sampling needle at the bottom of the sample 1 position, primary tube
type.
vertical collision flag starts moving upwards. Go 14 steps up again,
using the "-" key (numerical keypad). Save the value.

Service menu 59
Sampling needle 5 mm over sample 21 or 31 position (1st position of the

13 inner ring, depending on the sample tray type), primary tube type.
F6 checks the liquid level in the sample 21 or 31 position, sample cup
type.
Sampling needle at the bottom of the sample 1 position, sample cup
14 type.
15 Sampling needle at the bottom of the sample 21 position, sample cup
type (only for the 40 samples tray).
4.7.2.6 Reading position (ARM 1)
16 Calibration of the cuvette 1 in the reading position.

Place cuvette 41 at the center of the cuvettes window. In this way,
cuvette 1 is placed in the reading position. This position is roughly
calibrated. The accurate calibration is made with the F6 special func-
tion.
F6 finds the fine offset of the reading (same as TE-OS-F4-F6-1).
4.7.2.7 Wash station needles dispensation (ARM 1 and ARM 2)
17 Calibration of the wash needle 1 (the rightmost) over the cuvette 1.

F6 moves the needles down, just over the cuvettes.
Used to calibrate precisely the cuvettes offset so that the cleaning
needle can descend, in the next step, inside the cuvettes.
In this position also the sampling needle must be just over the dispen-
sing position. (Refer also to "5.6. Wash station down adjustment".)
4.7.2.8 Wash station needles down (ARM 1)
18 Wash needles down calibration at the bottom of the cuvettes (one step
over the bottom contact).
In this calibration row, the OP position cannot be calibrated.
F6 is a calibration procedure that approximately finds out the (115) WS_
ASP quote for correct aspiration. After auto-search, the suggested value
is displayed on the far right of row 18. Use the lowest value, i.e. 364 (red
marked) in the example:
60
4.7.2.9 Reaction rotor (ARM 1 and ARM 2)
19 Sampling needle at the bottom of the cuvette, inside the dispensing

hole, used by the 2nd dispensation.
F6 checks with the shock sensor the vertical position for 1st dispensati-
on, to give a rough adjustment of the (052) SN1DDS parameter.
If there is shock within 5 steps from (052) SN1DDS, the message "ErLow"
is displayed on the far right side.
If there is no shock within 20 steps, the message "NoShk" is displayed.
Else the message "Shock nn" with the number of steps to the shock is
displayed (red marked in the example):
4.7.2.10 Needle rotation (ARM 1 and ARM 2)

20 Sampling needle over the rotation wrap position (between 15 and 16).
It is used by the software to decide how to access to a reagent bottle,
clockwise or counter-clockwise.
F6 does the following three steps:
- Move the sampling needle to reagent position 15.
- Move the sampling needle clockwise to reagent position 16.
- Move the sampling needle counter-clockwise to the wrap position.

Service menu 61
4.8 Mechanical Check menu (TE-OS-F3)
Some precision and stress tests are executed for one or more motors at the
same time.
The duration of the tests in seconds is predefined but changeable. The tests are
partially long lasting.
At the end of the test or at aborting the test pressing ESC, a report of the errors
or the home position variations is displayed.
FIGURE 4-27
Mechanical checks menu (bottom
part)
Function keys:
F1 RN1/RN2/IP Rotates the sampling arms and the sample tray
forward and backward.
F2 SN1/SN2/OP Moves the sampling arms up and down and the
reaction rotor forward and backward.
F3 WS/SN1/SN2/OP F2 in combination with the down and up move-
ment of the wash station.
F4 OP+FS HOME Executes the home search of the reaction rotor
and the filter wheel, starting from all their posi-
tions. After positioning of the reaction rotor and
before starting the home search, the wash station
moves down and up. The analyzer shows how
many uncertainties of the motor are detected. At
that point it is possible to decide if to improve the
tention of the motor belt or improve the motor
pulley position.
F5 ALL Executes the four F1, F2, F3 and F4 tests.
At the beginning it requests the duration of the F1,
F2 and F3 tests in seconds.
F6 OP READING MOV Tests the reaction rotor movement. The instru-
ment simulates the real movement that perform
during the reaction reading. There it is possible to
find any lost step or any critical rotation.
F7 DS1/DS2 Tests the function of the dilutors. Here it is possi-
ble to verify the steps of the diluters motors and
the diluters limit switches.
62
F8 FS INVERSION Verifies if the filter wheel is loosing steps during

the inversion. The motor moves forward and back-
ward during the run and it is very important that
at the inversion moment no steps will be lost.
F9 OP BELT Tests the tension of the reaction rotor's motor belt.
The test suggests if the tension of the belt needs to
be increased or decreased.
F10 OP INVERSION Tests the reaction rotor's motor and belt. Verifies if
steps are lost during the inversion. The motor
moves forward and backward during the run and
it is very important that at the inversion moment
no steps will be lost. This test reduces the delay of
the inversion step by step, starting from 40 cs to
1 cs.
4.8.1 MECHANICAL CHECKS (TE-OS-F3-F1 TO TE-OS-F3-F5)
FIGURE 4-28
Example of the F5 ALL report
! For the checks F1 to F3 a loss

of ± 1 to 2 steps is acceptable.
IP and OP motors should not loose
any steps.
Explanation of the F4 results check:
1 Duration of function test F4 in minutes:seconds.

2 Number of cycles. 1 cycle = Search position of cuvette X, wash unit down,
read filter 1 (340 nm) and wash unit up.
80 cycles = one revolution of the reaction rotor.

Service menu 63
3 Number of steps lost per cycle / total steps lost.

4 Number of the cuvette that had more mechanical uncertainties.
5 Number of filter wheel steps lost.
6 Number of wash station down/up movement errors.
7 Pre-home position (reduce speed to precisely approach the reaction
rotor home position sensor).
8 Home sensor position. No steps should be lost in the reference zone
from 7 to 8.
9 Number of lost steps for every filter position. Should always be zero.
4.8.2 OP READING MOVEMENT (TE-OS-F3-F6)
FIGURE 4-29
Example of the F6 OP reading
movement report
The instrument simulates the real movement that performs during the reaction
reading. There it is possible to find any lost step or any critical rotation.
4.8.3 DILUTERS CHECK (TE-OS-F3-F7)
FIGURE 4-30
Example of the F7 diluters check
report
Here it is possible to verify the steps of the diluters motors and the diluters limit
switches.
64
4.8.4 FS INVERSION CHECK (TE-OS-F3-F8)
FIGURE 4-31
Example of the F8 FS inversion
check report
The motor moves forward and backward during the run and it is very important
that at the inversion moment no steps will be lost.
4.8.5 OP BELT CHECK (TE-OS-F3-F9)
FIGURE 4-32
Example of the F9 OP belt check
report
The test suggests if the tension of the belt needs to be increased or decreased.

Service menu 65
4.8.6 OP INVERSION CHECK (TE-OS-F3-F10)
FIGURE 4-33
Example of the F10 OP inversion
check report
The motor moves forward and backward during the run and it is very important
that at the inversion moment no steps will be lost. This test reduces the delay of
the inversion step by step, starting from 40 cs to 1 cs.
66
4.9 Reading Test menu (TE-OS-F4)

When the Optical Reading Test menu opens, it starts with filter 0 (blank) se-
lected. The cursor is at the filter selection position in order to select a filter.
FIGURE 4-34
Optical reading page
Function keys:
F1 SET O.D. REFER. Store the current A/D reading value in the refe-
rence value.
The O.D. value is the optical density calculated bet-
ween the current A/D value and the stored refe-
rence value.
F2 SELECT CUVETTE In the Reading Cuvette field, type the reaction cell
number (1 to 80) which should be used for the rea-
ding test. In position of cuvette 32, the two holes
for the adjustment of the gain and the offset of the
optical preamplifier are placed over the two trim-
mers to allow the access with a fitting screwdriver.
F3 CHANGE CUVETTE Positions the selected cuvette at the center of the
cuvette replacement hole - for cuvette inspection
or replacement.
F4 READ ALL FILT. Read and display all the autozero values of the fil-
ters.
F5 AUTOZERO Opens the autozero submenu.
F6 OP RDG. OFFSET Opens the reaction rotor reading offset submenu.
F7 FS WHEEL TEST Opens the filter wheel tests submenu.

Service menu 67
F8 CLEAR ERRORS Clears the display of the error message.

F9 STATUS CUVETTES Checks the status of the reading cells.
G: Gain adjust Selects cuvette 32 for the reading position to get
access to the gain and offset trimmers.
Z: Gain check Selects cuvette 0. I. e., it selects the gap between
cuvettes 32 and 33 for an autozero reading in air.
S: Optical gain Hidden function to change the optical gain value.
L: Lamp V.Level Hidden function to change the optical lamp vol-
tage level.
... Edit system parameters pressing the "." key three
times.
Filter table columns:
Column 1 Number
Column 2 Wavelength in nm.
Column 3 Previous reading in Abs.
Column 4 Current reading in Abs.
Column 5 Difference in Abs. between previous and current reading.
Column 6 v = valid value.
Err = error, the value is out of range.
Dif = error, non repetitive.
Ovf = reading overflow.
Column 7 Current autozero reading or initial value of the autozero. It helps
to evaluate the filters downgrade.
The factory initial autozero values are stored in the CPU's memory
and can be retrieved by the command OZ9<Enter>.
Column 8 Virtual column on the right side, outside the table. Shows the "F4
Read All Filters function" values.
4.9.1 READ ALL FILTERS (TE-OS-F4-F4)
To get a reading of all filters for a selected empty cuvette in "column 8" of the
filters table.
If a cuvette 1 to 80 is selected, the reading is executed through the cuvette. So
the A/D value is lower because of the cuvette's absorbance. The reading through
cuvette 0 (gap between two cuvettes) is higher.
68
If filter and cuvette 0 are selected, the reading simulates an autozero reading. It
is faster then the command TE-OS-F4-F5-1 Autozero, because it reads only ones
and not 10 times.
FIGURE 4-35
Example of F4 Read all filters
4.9.2 AUTOZERO (TE-OS-F4-F5-1)
F5 AUTOZERO, 1:Autozero: Executes the autozero of the optical group.
FIGURE 4-36
Autozero
At the end of the autozero processing, a confirmation is requested. "1" stores the
current values in the old values,"0" restores the old values.
Example of autozero with Dif error:
FIGURE 4-37
Autozero with Diff error
The analyzer could not obtain two autozero readings with the same value.
In case of Dif error, it is necessary to find out the origin of the unreliability:
1. Defective lamp, loose lamp screw
2. Lamp connections
3. Defective or broken or loose interference filter (TE-OS-F4-F5-2)

Service menu 69
4. Filter wheel offset (TE-OS-F4-F7-2)

5. Reaction rotor offset (TE-OS-F4-F6-1)
6. Filter wheel belt (4.8. Mechanical Check menu (TE-OS-F3))
7. Reaction rotor belt (4.8. Mechanical Check menu (TE-OS-F3))
If only filter 1 has the Dif Error, check points 1 and 3.

If generally only one filter has the Dif Error, check point 3.
4.9.3 AUTOZERO REPEATABILITY TEST (TE-OS-F4-F5-2)
F5 AUTOZERO, 1:Test: Repeats ten times the autozero, calculates the average
and the CV% for every filter.
FIGURE 4-38
Autozero repeatability test
The Old row displays the last autozero values executed before the test. It is only
a reference and is not part of the test.
The CV% row displays for each filter the CV% variation coefficient of the ten
autozero repetitions.
Good values for the CV% are lower than 0.100 % for all the filters and lower than
0.150 % for filter 1.
70
If the CV% values are higher, the test must be evaluated in the following order:
1. FS OP
The "FS" column displays the variation in the home positioning of the filter
wheel. All values in the column must be zero. If not, the filter wheel must be
checked. The "OP" column displays the variation in the home positioning of the
reaction rotor. All values in the column must be zero. If not, the reaction rotor
must be checked.
2. Average O.D.
The "Aver." Column displays the average of all the autozero values. The "O.D."
column shows the O.D. of the average compared to the old average value. The
values of this column must not vary more than 0.0020 Abs. If the values have a
constant drift, the optical signal is not yet thermally stabilized and it must be re-
peated. If the values have a random variation, there is noise in the optical signal
(optical preamplifier, optical cable, CPU A/D converter) or the lamp contacts are
bad or oxidized (check the contacts, spray with contact cleaner).
3. DARK
The "Dark" column displays the value of the optical signal with the solid dark
filter. It must be in the range 10 to 100. If negative, it is suggested to increase
the dark (offset) to a positive value, acting on the Offset trimmer (Refer to "5.2.2.
Offset adjustment" and "5.2.3. Gain adjustment"). If the dark value in the ten
repetitions varies more than two units, the optical preamplifier of the CPU A/D
converter must be checked.
4. Single filter problems

If the previous checks are ok, the single CV% of the filters must be checked to
see if there is a problem on a specific filter. If only one or a few filters have a bad
CV%, it is possible that there is a problem on that filter (loose locking ring, cra-
cked filter or cracked quartz gray filter).
5. Summary
To recapitulate, if the CV% values are high, it is recommended to check the fol-
lowing points:
- The TE-OS-F3-F4 OP+FS Home check gives information on the precision of
the home positioning.
- TE-OS-F4-F7-2 FS offset: If the filters are not centered on the light beam, the
light variation and the CV% increase.

Service menu 71
- TE-OS-F4-F6-1 OP offset: If the cuvettes are not centered, the light variation
and the CV% increase.
- Defective lamp, loose lamp screw.
- Defective or oxidized lamp connections.
- Defective or broken interference filter, cracked gray filter, loose locking ring.
- Optical preamplifier, optical cable, CPU A/D converter.
- TE-OS-F3-F4 OP+FS Home: Filter wheel belt check.
- TE-OS-F3-F4 OP+FS Home: Reaction rotor belt check.
4.9.4 REACTION ROTOR READING OFFSET (TE-OS-F4-F6)
To adjust the best reading points through the cuvettes and through the gaps
between the cuvettes.
FIGURE 4-39
Best reading points
This test has two options:
FIGURE 4-40
Fine and rough offset
4.9.4.1 Fine offset adjustment (TE-OS-F4-F6-1)
The procedure verifies if the current reaction rotor reading offset is correct or
must be modified. It verifies only the fine tuning and it is supposed that the
offset is already set within the range of the cuvette 1 (next procedure ROUGH
OFFSET). The test shows the current value of the reading offset (022) OPOFRD
and the suggested value. If the values are the same, the value of (022) OPOFRD
is not to be changed.
The +½ step is just to show the exact offset. It must of course be ignored when
updating the (022) OPOFRD parameter.
72
If the values are different, the service engineer can type Y to store the new value
in (022) OPOFRD or N to keep the old value.
4.9.4.2 Rough offset adjustment (TE-OS-F4-F6-2)
It is used to find the rough reading offset of cuvette 1. The purpose it to adjust
the reaction cuvette 1 position in the center of the cuvette access opening in the
reaction rotor cover to ensure that the cuvette 1 will be used by the fine offset
adjustment.
FIGURE 4-41
Rough offset adjustment menu
4.9.5 FILTER WHEEL TEST (TE-OS-F4-F7)
FIGURE 4-42
Filter wheel test submenu
4.9.5.1 Test FS home repeatability (TE-OS-F4-F7-1)
The home positioning is repeated 100 times and a correlation check is perfor-
med.
FIGURE 4-43
FS home repeatability test

Service menu 73
The first two numbers are only for reference and must not be considered.
All the numbers in the rows with eight columns must be higher than 0.9990. In
this case the result is marked as -OK-
If the result is lower than 0.9990, the result is failing. In this case the result is
marked as FAIL. The final result is then "FAIL".
4.9.5.2 Adjust FS offsets (TE-OS-F4-F7-2)
FIGURE 4-44
FS offset adjustment
FIGURE 4-45
Best reading point
The test scans automatically all the filters searching for the light peak.
For each filter is displayed if a correction has been applied to the offset parame-
ters (031) FSOFS1, (032) FSOFS2, …, (038) FSOFS8.
! Filter 9 (additional filter) is
not checked in this test.
The value between the two asterisks is the currently set peak value, before the
correction performed by the test. If the peak is correctly detected, no correction
is performed.
74
If some of the readings are dispayed with a red background, the values are in
overflow (3,276) or too close to the overflow (higher than 1,940). The analyzer
will automatically get the filter reading in the best position out of the center to
avoid the overflow.
In this case it is suggested to reduce the optical gain (refer to "5.2.3. Gain adjust-
ment") and then repeat this test until there are no more red areas.
4.9.6 CUVETTES STATUS (TE-OS-F4-F8)
FIGURE 4-46
Cuvettes status
The upper table is the current reading (in 0.1 mAbs) of the 80 cuvettes at the
filter 1 wavelength of 340 nm.
The lower table is the difference of the current reading to the startup reference
reading (in 0.1 mAbs) of the 80 cuvettes at the filter 1 wavelength of 340 nm.
At the bottom there are some statistics on the 80 cuvettes:

- Min: Minimum reading.
- Aver: Average reading.
- D: Difference between minimum and average.
- Sdev: Standard deviation.

Service menu 75
4.10 Washing menu (TE-OS-F5)

FIGURE 4-47
Washings page
1 The wash position is the position of the reaction rotor that is assigned to
operate under the first (rightmost) needle of the wash station. It ranges
from 1 to 80.
2 On the upper right corner: Continuous monitoring of temperature, floats
status and cover safety switch.
!
3 The reaction cells wash status bar. It displays the status of the 80 reaction If an error appears, the error
cells: message is displayed white-
0: Clean cell. on-blue in the line above the sta-
1: Dirty cell. tus bar 3.
2 to 9: Cell passed wash station 1 to 8 wash cycles.
F: Wash failed 1 or 2 times (the cell didn't pass the reading test after fill in
wash cycle 6).
W: Cell in warning to be excluded (wash failed 3 or 4 times).
X: Cell excluded (wash failed for 5 times).
The white-on-blue-background group of eight cells represents the eight
positions of the wash station. The first on the left is position 8 of the
wash station, the last on the right is position 1 of the wash station.
4 The pumps and lamp working hours.
The pumps dispensation in μl (last, average, flow rate, timers, pump sta-
tus).
Function keys:
F1 WASH POSITION Selects the current wash position.

F2 WASHES Runs the wash station procedure.
76
F3 FILL W.CUVETTE The cuvette selected by the wash position will be filled
with water through the sampling needle.
F4 STARTUP Executes the startup procedure.
F5 PUMPS TESTS Opens the pumps tests submenu page.
F6 WASH NEEDLE Executes a needle wash cycle in the wash well.
F7 SING.WASH CYCLE Executes a single cycle of the wash station and moves
the wash position to the next cell.
F8 CLEAR ERRORS Clears the display of an error message.
F9 SPECIAL WASH Executes the Rinse Cuvettes procedure.
4.10.1 WASH POSITION (TE-OS-F5-F1)
To select the current wash position, enter its number and confirm with <Enter>.
FIGURE 4-48
Select wash position
The wash station indicator moves with its rightmost position (wash needle 1) to
the selected position.
4.10.2 EXECUTE WASHES (TE-OS-F5-F2)
FIGURE 4-49
Execute washes
Select a number of washes (1 to 80) to set a number of reaction cells as dir-

ty. When the cell passes under the wash station, if is dirty (status 1), the wash
procedure is activated. If the cell is already clean (status 0), the wash station
operations are skipped.

Service menu 77
Press F2 to activate the continuous wash function.

The reaction cells of the rotor are washed in sequence. For each reaction cell, the
new reading value of water at 340 nm after the wash cycles is displayed in units
of 0.1 mAbs in the "Cuvettes zero at filter 1 (340 nm)" table.
Press ESC to stop the washing function.
4.10.3 FILL WASH CUVETTE (TE-OS-F5-F3)
Fill the cuvette selected by the wash position with water through the sampling
needle.
4.10.4 STARTUP (TE-OS-F5-F4)
Execute the startup procedure in 3 different ways:

1. Quick Start-up (no fill).
2. Full Start-up with fill and test.
3. Quick Start-up (no fill). Only dirty/excluded tubes will be washed.
4.10.5 SPECIAL PUMPS TESTS (TE-OS-F5-F5)
FIGURE 4-50
Special pumps tests menu
4.10.5.1 Test pumps (TE-OS-F5-F5-1)
FIGURE 4-51
Test pumps
78
For the pumps test needs, it is requested a primary tube filled with 5 ml of water
in sample position 1. If it is missing, the procedure is halted.
The pumps test performs a precise measure of the flow of the six dispensing
pumps of the wash station and the needle wash dispensing pumps. It also
checks the wash station vacuum pump.
The results of the test can be verified in the monitoring section of the mainte-
nance.
The test is performed in four steps:
1 It calibrates the level sensor in the reaction cuvettes.

2 Test the wash station needles aspiration evaluating the vacuum pump
flow. If the reaction cuvettes are not empty at the second step, the test
fails.
3 Test the water dispensation from the needle.
4 Test the dispensation of the six pumps of the wash station.
5 According to the new calibration of the level sensor in the reaction cells –
(278) OPL150 and (279) OPL450 – the analyzer asks – if necessary – if you
want to adjust the dispensing quote (053) SN1DS2 and (369) SN2DS2.
Type Y to adjust, N to keep the old value.
Update (278) OPL150 and (279) OPL450:

The level sensor calibration has been updated.
The vacuum pump aspiration test left some water volume at the first quick pass,
this is the normal condition. At the second pass the test is OK.
Sampling needle internal washing flow-rate measured.
Wash station dispensing pumps flow-rate measured.
Request to update the dispensing SN1DS2 position.
Pump 1 and 2 have always a lower dispensation.
4.10.5.2 Fill hydraulic tubes – the pumps self-test (TE-OS-F5-F5-2)
This procedure is used to fill all the hydraulic tubes, after tank replacement or
for the initial filling.
In the HI software, there are three options:

- Fill water tubes, after water tank replacement.
- Fill wash tubes, after wash tank replacement.
- Fill all hydraulic system.

Service menu 79
In the TE-OS-F5-F5 Pumps Tests menu only the fill all option is possible.
To avoid dangerous water overflow caused by aspiration pumps malfunctioning
or unpredictable results caused by wash dispensing failure, during the fill hy-
draulics procedure a self-test of the pumps is performed.
The self-test is performed mainly to check if the aspiration and vacuum pumps
are performing. It also checks the flow rate of the needle wash dispensation,
both internally and externally.
The self-test is based on the use of the level sensor. So the first step of the self-
test is to check the level sensor in the diluent bottle. If the diluent bottle is em-
pty, the procedure is halted.
FIGURE 4-52
Fill tubes + pumps selftest
The fill hydraulics procedure performs the following steps:

- Well tubes filling using the external needle wash flow, stop when water is
flowing.
- Well tubes filling using the internal needle wash flow, stop when water is
flowing.
- Check and measure the flow of the well pumps (external needle, internal
needle, aspiration).
- Fill six reaction tubes with 350 μl of system solution.
- Check the wash station vacuum pump with a double-step aspiration, evalu-
ate the vacuum flow rate and a final test result PASS/FAIL.
- Wash solution tubes filling, using the pump 2 and needle 2 of the wash stati-
on. Every 5 cycles, check with the level sensor if the wash solution is flowing.
- Internal tubes filling, repeating several cuvettes washes to complete the fil-
ling of the wash station tubes with the system solution (needles 1, 3, 4, 5,
6). Every 5 cycles, check if water is flowing from needle 6. When the water is
flowing regularly from needle 6, the procedure stops.
- Fill the tubes of the needle wash.
- Measure the flow rate of the needle wash (internal, external, aspiration).
80
- Fill reaction tubes with 350 μl system solution.

- Test vacuum pump for wash station aspiration (PASS / FAIL).
- Fill wash solution tubes through wash station needle 2.
- Fill system solution tubes.
4.10.5.3 Empty tubes (TE-OS-F5-F5-3)
Performs the same procedure as fill hydraulic tubes, except that its duration is
fixed and it doesn't check when the tubes are filled.
Extract the floats from the tanks before executing the empty tubes procedure.
Do not unplug the external tubes from the analyzer until the procedure is com-
pleted.
4.10.5.4 Check wash station residue (TE-OS-F5-F5-4)
This procedure can be performed to verify the liquid residue left by the wash
station inside the reaction cells.
The test is performed in the following way:

- Fill 10 reaction cuvettes with 200 μl of dye.
- The 10 recation cuvettes are washed.
- The 10 washed reaction cuvettes and 6 reference reaction cuvettes are filled
with the diluents and read. The average reading of the 10 and the 6 is dis-
played.
4.10.5.5 Adjust pumps (TE-OS-F5-F5-5)
This test measures precisely the volume dispensed by the five wash station peri-
staltic pumps and allows eventually to adjust the pump timers values to obtain
the specified dispensation volume.
The measuring is repeated six times and then a report is displayed:

- Minimum volume dispensed.
- Difference between minimum and maximum volume dispensed.
- Average volume dispensed.
- Old pumps timers.

Service menu 81
- Suggested adjusted pumps timers, to correct the dispensed volumes to re-

ach the (177) WSHVOL parameter value (default: 400 μl).
- The pumps timers range from a minimum of 40 to a maximum of 80. Values
outside this range will we clipped to the minimum and maximum values.
- Press Y to adjust the pumps timers, else to keep the old values.
FIGURE 4-53
Adjust pumps
4.11 Diagnostics menu (TE-OS-F6)

FIGURE 4-54
Diagnostics menu
Here it is possible to find the main tests that are in the previous pages. It is use-
ful to perform these diagnostics tests in order to improve the optical path or in
order to calibrate and verify all pumps, all dispensations, all aspirations.
Furthermore here it is possible to perform all the sequence tests by F9 and save
automatically all screenshots on the desktop. If you want to save the screens-
hots automatically, you must set the parameter (205) SVSCRN to 1.
82
4.12 Edit Parameters menu (TE-OS-F7)
In this procedure, an entry password can be activated by the installer.

For details please refer to the Human Service Bulletin HSB0001.
The system parameters edit pages are called from terminal with the command
OP<Enter> or from the TE-OS-F1 Hardware Test, TE-OS-F4 Reading Test and TE-
OS-F5 Washings menus pressing three times the dot "." key.
FIGURE 4-55
Parameters menu
Parameters menu function keys:
F1 SELECT PARAM. Locate a parameter by its number.

F2 / PG UP NEXT PAGE Go to the next page.
F3 / PG DN PREVIOUS PAGE Go to the previous page.
F4 LOCK/UNLOCK Unlock/Lock the parameters values for being
editable.
F5 FIND PARAMETER Locate a parameter by its name.
F6 BACKUP PARAM Backup all parameter settings.
F7 RESTORE BACKUP Restore all parameters settings from the last
backup.
F8 SCREEN SHOTS Save a series of screenshots of all parameter
pages on the desktop.
Exit the Edit Parameters pages by pressing <ESC>. Following menu is shown:
FIGURE 4-56
Parameters exit menu

Service menu 83
Exit menu function keys:
F1 No save All changes will be rejected.

F2 Save parameters on EEPROM All changes will be stored.
F3 Restore EEPROM Last stored parameter will be loaded back.
F4 Restore defaults Avoid this option, because all calibrations
and many test procedures have to be re-
peated.
4.12.1 PARAMETER PAGES
The name of the parameter pages is displayed in the upper right corner.
There are 9 parameters pages:

1. IP, OP, FS
2. SN Needle, DS
3. REAG, WASH STATION
4. SPEEDS
5. WASH PUMPS
6. MISCELLANEOUS
7. LEVELS, FLOATS
8. TEMP., DILs, PIPETTING
9. READINGS
10. SECOND SAMPLING ARM
11. ACCELERATIONS
Every page shows 40 parameters, divided in two tables of 20 parameters each

with following column:
Column 1 The parameter number.

Column 2 The parameter name.
Column 3 The parameter type (P, U, I, O, R, S, A, V, C).
Column 4 The current parameter value.
Column 5 The default parameter value in parenthesis (), if the current value
is not equal to the default value.
Trying to input a number outside the parameter's values range causes a beep,
the new value is rejected and the old value is kept.
A blue field indicates a parameter which you are allowed to change.
84
A blue reverse field indicates a value which has not yet been saved in EEPROM.
A red field indicates a parameter which you must not change.
4.12.2 SELECT PARAMETER (TE-OS-F7-F1)
FIGURE 4-57
Select a parameter by its number
Type the number of the parameter to be found (3 digits with leading zeros). The
cursor will move to the selected parameter.
4.12.3 GO TO NEXT/PREVIOUS PAGE (TE-OS-F7-F2/TE-OS-F7-F3)
The keys work in a loop, i.e.:

- F2 (next) moves from page 11 to page 1.
- F3 (previous) moves from page 1 to page 11.
4.12.4 UNLOCK/LOCK PARAMETERS (TE-OS-F7-F4)
The parameter values shown in blue cannot be edited if the status in the upper
left corner is Read only. With the status changed to Unlocked the parameter
values can be edited.
4.12.5 FIND PARAMETER (TE-OS-F7-F5)
FIGURE 4-58
Select a parameter by its name
Type the name of the parameter to be found. The cursor will move to the se-
lected parameter.
4.12.6 BACKUP PARAMETERS (TE-OS-F7-F6)
Press F6 to backup all current system parameters values. A confirmation is re-

quested.

Service menu 85
FIGURE 4-59
Backup all parameters
Confirm with Y to backup all parameters. A previous backup of all parameters

will be overwritten.
4.12.7 RESTORE PARAMETERS (TE-OS-F7-F7)
Press F7 to restore the parameters values recorded during the last backup.
A confirmation is requested after typing three times 0 (zero):
FIGURE 4-60
Restore all parameters
Confirm with Y to restore all parameters from the previous backup.
4.12.8 SCREENSHOTS OF ALL PARAMETERS (TE-OS-F7-F8)
Press F8 to save a set of all parameters screenshots on the desktop Terminal

Autoscreen folder.
To enable this function the parameter (205) SVSCRN has to be set to 1.
4.12.9 SAVE THE PARAMETERS LIST AS A TEXT FILE
To create a parameters list file:

- Go to TE-OS.
- Select the "Log" tab and mark the "logs" check box.
- Select the "Terminal" tab and type the command PL<Enter>.
- Wait for the end of the parameters list (last parameter: CODCHK).
- Select the "Log" tab again. The list of all the parameters must be there.
- With a right mouse click save the parameters list as a .TXT file.
- Close the Service Terminal.
- Open the created parameters list file with Wordpad or with Word.
4.13 Clear Errors menu (TE-OS-F8)
To clear the display of an error message.

86

Adjustments 87
5 ADJUSTMENTS
5.1 Reaction rotor temperature
The procedure is to calibrate the temperature sensors so to match an exter-

nal thermometer, using the TE-OS-F1 Hardware Test menu or the TE-OS-F5
Washings menu.
FIGURE 5-1
Thermometer probe placement
The calibration requires a thermometer with a 3 mm probe to measure the tem-

perature inside a reaction cell of the outer plate.
Fill the reaction cell at the center of the access hole to the reaction cells with
0.5 ml of water.
Insert the thermometer probe in this reaction cell.
Check the temperature by either:

- TE-OS-F1-F5 Display Inputs.
In the window in the upper right corner the row OP Temp indicates the OP
temperature in tens of degree Celsius.
or:
- TE-OS-F5 Washings menu.
The OP temperature is displayed in degree Celsius in the upper right corner.
The sensor is in a feed-back loop for temperature control, so the system will al-
ways slowly drive the temperature to the same (apparent) reference.
If the measured temperature is higher than the set-point, decrease the value of
the parameter (285) OPTCAL so that the displayed temperature goes close to the
measured temperature and wait for stabilization.
If the measured temperature is lower than the set-point, increase the value of
the parameter (285) OPTCAL so that the displayed temperature goes close to
the measured temperature and wait for stabilization. The procedure must be
repeated sometimes until the displayed value is identical to the measured value.
88
Adjust the parameter (285) OPTCAL until the displayed OP temperature and an
external thermometer temperature, placed in the reaction cell, are the same.
5.2 Optical offset and gain
The optical preamplifier board is located in the inner side of the reaction rotor.
The two trimmers for gain and offset are accessible in TE-OS-F4 Reading Test
menu selecting the cuvette 32 with the F2 Select Cuvette key or with the rea-
ding cells selection shortcut keys G and Z.
FIGURE 5-2
Access holes for Gain and Offset
adjustment
Left hole (from the front) is to adjust the gain trimmer.

Right hole is to adjust the offset/dark trimmer.
Offset and gain adjustment must be made after the thermal stabilization of the
optical group, the reaction rotor and the optical preamplifier. Wait at least 15
minutes.
5.2.1 NECESSARY TOOLS
Flat screwdriver 1.5 mm, with minimum 22 mm length, for the trimmers
(16890/171).
5.2.2 OFFSET ADJUSTMENT
! The offset value has to be al-

ways positive.
Select filter 0 (dark) pressing the 0 (zero) key.
Check the reading and adjust the offset with right trimmer so that the value is
approximately 750 units (range between 500 and 1,000).

Adjustments 89
5.2.3 GAIN ADJUSTMENT
During the reading test, the operator can select all the filters with the keys 0 to
9 and get the corresponding reading.
Check on the auto zero readings list which is the filter with the highest rea-
ding and select this filter pressing the numeric key corresponding to the filter
number.
Adjust the gain with left trimmer that the maximum reading of the highest
(most transparent) filter is close to 56,000 and the lowest one (least transpa-
rent) is higher than 40,000. Note that the maximum reading of the A/D con-
verter is 65,000. The adjustment of the gain can also be done by parameter
(341) OPGAIN. It is possible to increase or decrease the value in order to improve
the filters values. The default value is 75. This process is very useful in remote
control (e.g. by TeamViewer).
In TE-OS-F4 Reading Test menu press S to modify the parameter (341) OPGAIN.
Since cuvette 32 is selected, the current reading is obtained with the light beam
through it. Depending on the absorbance of this cuvette, the A/D reading during
the adjustment of the trimmers is 10 % lower (or even less) than the real auto-
zero value. To check the true A/D reading, take the screwdriver off the holes and
select cuvette 0 with the F2 Select Cuvette key. Press the F4 Read All Filters key
to get a quick reading of all wavelengths.
In the A/D reading of the bare beam (cuvette 0 selected), it is preferable not to
go beyond 60,000 because it is needed some margin of tolerance to avoid rea-
ding overflow when changing the lamp. Lamps can have an emission tolerance,
mainly in the low wavelengths range (340 to 400 nm).
When all A/D reading values are satisfactory, press the F5 Autozero key and then
the 1 Single Autozero key.
Verify that the difference between all filters must be lower than 300 units to
avoid problem when you replace the lamp. In the TE-OS-F4 Reading Test menu
it is possible to press "A" to perform the automatic gain adjustment. The instru-
ment will set the highest filter to 56,800 units and will run the autozero. Wait
for the end of the procedure.
90
5.2.4 READING CELL SELECTION SHORTCUT
In the TE-OS-F4 Reading Test menu:

- Press the G key to select cuvette 32 for the adjustment of the trimmers with
the screwdriver.
- Press the Z key to select cuvette 0 (space between the cuvettes 32 and 33) to
check the reading at the true range.
5.2.5 INTERFERENCE FILTERS
The interference filters are factory equalized, so that the maximum difference in
the A/D readings is typically lower than 20 %.
Anyway, to reduce the difference between the final transmittances, it is possible
that a light quartz grey filter has been mounted on some interference filters for
finer matching. Grey filters are available for 38 mAbs and 100 mAbs.
The difference between all filters must be lower than 700 units.
Interference filters have a very high stability and are mechanically protected. It
is very uncommon that they must be replaced. But it is eventually possible that
a quartz grey filter has cracked if it has been locked too tight by the black locking
ring.
5.2.6 READINGS OUT OF RANGE
If a filter has an autozero reading out of range, all the methods employing this
filter will be marked with AUTOZERO ERROR and these methods cannot be exe-
cuted.
The filter 1 (340 nm) is also used for the check of the cuvettes washing. An au-
tozero error on this filter will give an AUTOZERO ERROR during the washings.
5.3 Filter wheel
#2 mm Allen key (for M3 Allen screws)


Adjustments 91
5.3.2 OPTICAL HOME SWITCH ADJUSTMENT
To check if the filter wheel is operating properly, there are two basic tests to be
executed in this sequence: ! The optical limit switch must
never be moved. Only if it is
broken. All the adjustments will
1. TE-OS-F4-F7-1 FS Home Repeatability. be done without moving the opti-
2. TE-OS-F3-F4 FS Home Test. cal switch.
3. TE-OS-F4-F7-1 FS Home Repeatability.
The first test checks if the mechanics are working properly and if the stepper
motor is well phased on the optical limit switch. If all the results are not OK
(excluding the first row with two numbers), once you have excluded a big pro-
blem in the mechanics, it is possible that the home positioning is not repeatable
because the optical limit switch position is phased in the middle of two mecha-
nical steps.
In this case, the cogwheel of the FS motor must be shifted a bit. This is a blind
operation, because you can check the result only later, repeating the FS Home
Repeatability test.
1. Open the filter wheel cover (Allen screw M5 placed over the FS motor).
2. Release with a #2 Allen Key the two M3 Allen screws on the cogwheel of the
FS motor.
3. Rotate just a bit the cogwheel on the motor axis.
4. Tighten the two Allen screws of the motor's cogwheel.
5. Repeat the TE-OS-F4-F7-1FS Home Repeatability test. If it fails, go back to
step 2.
6. At the end, close the filter wheel cover.
5.3.3 FS BELT ADJUSTMENT
The second test, FS Home Test, will check the Home search from all the filter
positions. When the filter wheel is operating perfectly, it must give all 0 (zero)
values.
If it gives non-zero values higher than 2, the filter wheel belt must be tightened.
To tighten the belt, release the four M4 Allen screws of the motor, pull with one
hand the motor, sliding outward, to tighten the belt and then tighten the four
motor screws with the other hand.
92
5.3.4 OFFSET ADJUSTMENT
Once the FS positioning is accurate and repeatable, the offsets of the single fil-
ters must be detected. This operation is done automatically by the instrument
running TE-OS-F4-F7-2 Adjust FS Offsets.
5.4 REACTION ROTOR BELT TIGHTENING
#3 mm Allen Key (for M4 Allen screws)
5.4.2 OP BELT TIGHTENING
To tighten the OP belt, follow the procedure:
1. Take off the sample tray and the instrument upper cover.
2. Move the reaction rotor with the hand that you can identify the OP motor.
3. Using the Allen Key, loosen the 4 screws that hold the OP motor.
FIGURE 5-3
Unscrew the OP motor screws

Adjustments 93
4 - While keeping the belt well tighten pulling the OP motor, tighten the four
motor's screws.
FIGURE 5-4
Fasten the OP motor belt
5.4.3 ACCESS TO THE OP MOTOR SCREWS
The 4 screws are placed on a square of approximately 5 cm. Two screws are
placed on the internal side and two screws are placed on the external side.
To find the internal screws it is necessary to move the IP and OP plate together.
To have access to the 4 OP motor's screws:
FIGURE 5-5
First internal screw
FIGURE 5-6
Second internal screw
The 2 outer screws are accessible rotating the reaction rotor in such a way that
the hole placed on the bottom of the rotor will be, in sequence, exactly over the
2 screw heads.
To find the external screws it is necessary to move just the OP plate.
94
FIGURE 5-7
Third external screw
FIGURE 5-8
Fourth external screw
The 2 inner screws are accessible rotating first the sample rotor in such a way
that the hole on the IP plate will be overlying on the vertical of a second hole of
the reaction rotor (the IP hole and the OP hole must be then on the same radius).
Move then the IP and OP rotors over the heads of the 2 inner screws of the OP
motor.
5.5 Sampling needle vertical adjustment in the wash well
Select calibration step 09 in the TE-OS-F2 Mechanical Calibration menu. The

needle goes down in the wash needle position.
Press F6 to enter the special check function and then press the space bar to step
through the check:
1. The water springs out from the needle and washes the needle internally and
externally. Check if the water flows well ((196) INWELV = pump speed).
2. The flow stops and the needle moves up to the drip position. The needle must
stay about one millimeter inside the water drop (the bottom of the needle must
be at the same level of the top of the white well nipple (adjust (045) SN1WSH).

Adjustments 95
3. The water flows from the lateral nozzle and washes the needle externally.
The water jet must hit the needle gently with a downward parabola and not hit
horizontally. In this way the drops will not remain attached on the needle side
(adjust (195) EXWL1V and (194) EXWL2V).
When the F6 special check function is completed, with the +/- keys you can ad-
just the vertical position of the needle inside the well that, when it goes up to
the drip position, the needle remains 1 mm inside the water drop.
Adjustment of the needle in the wash well via remote control:
1. Select calibration step 09 in the TE-OS-F2 Mechanical Calibration menu.
2. The needle goes down in the wash needle position.
3. At this point press the "+" key to go down until the shock sensor is engaged.
4. Go back 50 to 60 steps pressing the "-" key.
5. Press F2 to save this position.
5.6 Wash station down adjustment
5.6.1 ROUGH AUTOMATIC CALIBRATION
TTo be used only by operators not able to execute the FINE CALIBRATION proce-
dure.
Select calibration step 18 in the TE-OS-F2 Mechanical Calibration menu.
5.6.2 FINE CALIBRATION
Select calibration step 18 in the TE-OS-F2 Mechanical Calibration menu.

Use the "+" and "-" keys to move the wash station up and down.
Go down until the wash station white dryer touches the bottom of the reaction
cuvettes.
Then go up one step and press F2 to store the new calibration.
96

SERVICING 97
6 SERVICING
6.1 LAMP REPLACEMENT
The lamp life has a wide distribution. It can range from 500 to 2,000 hours, with
typical life 1,000 hours. ! Do not touch the glass part
of the lamp with your fin-
gers. Fat, dust and humidity shor-
1. Switch the instrument off. ten the life of the lamp and limit
2. Open the lamp window in the back side of the instrument. the lamp emission.
3. Unplug the lamp connector (A).
FIGURE 6-1
Lamp and connector
4. Unscrew the lamp knob (B).
FIGURE 6-2
Lamp knob
5. Remove the old lamp.

6. Insert the knob in the new lamp holder.
FIGURE 6-3
Lamp holder
7. Insert the new lamp and fasten the knob (B).

8. Plug the connector in (A).
98
9. Close the lamp window.

10. Switch the instrument on.
11. Wait 15 minutes for warming-up.
12. Go to "MAINTENANCE PROCEDURES", "Special" and "Halogen lamp" to open
the "Optical group" window.
FIGURE 6-4
Optical group window
13. Click the "Replace lamp" button to reset the lamp life timer and execute the
auto-calibration of the lamp. This procedure will adjust the mechanical off-
sets for the best optical performance and stores the autozero values in me-
mory.
If you are not in TE-OS:
14. Put the instrument in RUN to execute an autozero and then go

to the "Optical group" window again to check the lamp emission.
If the lamp has a correct emission (all wavelength readings are between
40,000 and 60,000), execute the instrument startup. Else go to TE-OS.
If you are in TE-OS:
15. Go to TE-OS-F4-F5-1 to execute the AUTOZERO procedure to check the lamp

emission. If the lamp has not a correct emission (wavelength readings are
not between 40,000 and 60,000), you have to adjust these values within this
HumaStar 100/200 | Service manual

SERVICING 99
range trimming the optical preamplifier gain. See "5.2.2 Offset adjustment"
and "5.2.3 Gain adjustment".
16. In TE-OS-F4-F7, verify the filter offsets with the function 2 "Adjust FS offsets"
and eventually adjust the filter offsets. See "4.9.5.2. Adjust FS offsets".
17. In TE-OS-F4-F6, verify the reaction rotor offset with the function 1 "Search
fine reading offset" and eventually adjust the reaction rotor reading offset.
See "4.9.4.1. Fine offset adjustment".
18. In TE you can run the command OZ6<Enter> to perform the automatic cali-
bration of the optical path and for the gain. The instrument will perform the
"Adjust FS offsets", the "Search fine reading offset" and will perform the gain
adjustment to set the higher filter(s) to 56,000.
19. Execute the start-up of the analyzer.
6.2 SAMPLE TRAY REPLACEMENT (40 / 60 POSITIONS)
There are two versions of sample trays available:

- 16890/10 "Removable sample tray, 60 positions". Standard sample tray.
FIGURE 6-5
Standard sample tray
- 16890/11 "Removable sample tray, 40 positions". Alternative sample tray.
FIGURE 6-5
Alternative sample tray
100
When installing a sample tray, following system parameters have to be confi-

gured in TE-OS-F7 Edit Parameters:
System parameters for the standard sample tray with 60 positions:

(000) IPNPS0: 30 positions on the outer ring.
(001) IPNPS1: 30 positions on the inner ring.
(008) IPTYPE: 2.
(310) NSAMPL: 7 (last three positions reserved for diluents/wash solu-
tions).
System parameters for the alternative sample tray with 40 positions:

(000) IPNPS0: 20 positions on the outer ring.
(001) IPNPS1: 20 positions on the inner ring.
(008) IPTYPE: 1.
(310) NSAMPL: 37 (last three positions reserved for diluents/wash solu-
tions).
Then in TE-OS-F2 Mechanical Calibrations the high and low position of the first
tube of the inner and the outer ring have to be calibrated for sampling arm 1
and sampling arm 2:
Calibration row 11:

- "H" is the sampling needle high position, 5 mm over a 110 mm sample tube
in position 1.
- Affected parameters for sampling arm 1:
(002) IP0SN1,
(090) RN1IP0,
(043) SN1MID
(390) IP0SN2,
(384) RN2IP0,
(362) SN2MID
Calibration row 12:

- "PTU" is the low position of primary tubes. Touch the bottom and go back
14 steps.
(002) IP0SN1,
(090) RN1IP0,
(051) SN1PTU

SERVICING 101

(390) IP0SN2,
(384) RN2IP0,
(368) SN2PTU
Calibration row 13:

- "H" is the sampling needle high position, 5 mm over a 110 mm sample tube
in position 31 (standard sample tray) or 21 (alternative sample tray).
(003) IP1SN1,
(091) RN1IP1,
(043) SN1MID
(391) IP1SN2,
(385) RN2IP1,
(362) SN2MID
Calibration row 14:

- "CUP" is the low position of the system cups mounted on the adapter. The
adapter is necessary to allow level detection with the cups. Touch the bot-
tom and go back 7 steps.
(002) IP0SN1,
(090) RN1IP0,
(050) SN1CUP
(390) IP0SN2,
(384) RN2IP0,
(367) SN2CUP
Calibration row 15:

- "CU2" is the low position of the Hitachi cups on the inner ring of the alterna-
tive sample tray. The inner ring of this tray can only hold these cups, without
adapter. Touch the bottom and go back 30 steps.
(003) IP1SN1,
(091) RN1IP1,
(054) SN1CU2
102

(391) IP1SN2,
(385) RN2IP1,
(370) SN2CU2
6.3 SINGLE REACTION CELL REPLACEMENT
Go to "MAINTENANCE PROCEDURES", "Special" and "Reaction cuvettes".
FIGURE 6-7
Reaction cuvettes window
Click on the cuvette to be replaced.

The rotor will move and bring the selected cuvette at the center of the opening.
FIGURE 6-8
Selected cuvette
You can verify that the engraved number corresponds to the selected one.
With the "Cuvette extraction pliers" 16890/54 (from the accessory box), extract
the cuvette placed at the center of the opening.
FIGURE 6-9
Reaction cuvettes replacement

SERVICING 103
Insert the new cuvette. The cuvette has a rectangular foot. It must be inserted
oriented so that the marks (one or two) on the bottom face are situated on the
external side of the rotor ring.
Confirm on the screen that the cuvette has been changed.
FIGURE 6-10
Reaction cuvettes replacement
confirmation
This will reset all the cuvette references and autozeros and mark the cuvette as
dirty, to enable its washing before it can be used in a reaction.
6.4 REACTION ROTOR REPLACEMENT
Disassemble the needles group of the wash station (two screws A and B) and
move it aside, but don't damage the TEFLON tubes.
FIGURE 6-11
Wash station needle group
replacement
Disassemble the black reaction rotor cover (three screws D, E and F) and remove
it.
FIGURE 6-12
Reaction rotor cover replacement
104
Unscrew the reaction carousel (six screws A, B, C, D, E and F) and remove it.
FIGURE 6-13
Reaction carousel replacement
Insert the new carousel taking care to keep the orientation (one pin marked with
a red circle in above figure).
Tighten the six screws progressively, taking care the rotor is the correct flat po-
sition.
Reassemble the reaction rotor cover and the wash station needle group.
6.5 Sampling needle replacement

Take off the Sampling arm cover
FIGURE 6-14
Sampling arm cover
Unscrew the needle holder cap
FIGURE 6-15
Needle holder cap replacement
Remove the metal needle screw.
FIGURE 6-16
Needle holder cap replacement

SERVICING 105
Detach the TEFLON tube and take off the needle.
FIGURE 6-17
TEFLON tube replacement
Pull up the old needle and put down the new one.
FIGURE 6-18
Needle replacement
After reassembling, check that the needle slides correctly in the needle guide
without friction, so that the spring holds the needle in the lower position and
the shock sensor is correctly activated in the upper position.
If the needle doesn't slide freely, try to loosen the white needle holder cap and
verify the spring position.
FIGURE 6-19
Needle holder and shock flag
components
A: Needle holder cap

B: Brass Flag
C: Needle Spring
D: Needle metal screw
106
6.6 Filter replacement and equalization
8 or 9 interference filters are mounted on the analyzer. Each filter's wavelength

is defined in the system parameters (320) FILTR1 to (328) FILTR9.
The wavelength defined in the system parameters is detected by the HI software
when the analyzer is connected.
The filter wheel has ten positions. Position 0 is for the dark offset reading. Every
interference filter is marked with the corresponding number engraved.
FIGURE 6-20
Filter wheel
The filters are equalized (matched) so that the reading of each filter is within
a ±20 % difference in relation to the 340 nm reference filter. Typical final ad-
justment of the Autozero of the filters is a maximum of 56,000 units and a mi-
nimum of 36,000 units but the difference between them must be lower than
10,000 units.
The filters supplied belong to a set of 8 or 9 factory matched filters. This me-
ans that, typically, the filters don't need a strong equalization with dark gray
filters or pinhole disks. It can happen that light gray quartz filters of 38 mAbs, or
100°mAbs (in any applicable combination) are added to the filters with a higher
transmittance to reduce their emission and to have a better equalization.
1. Remove the instrument cover.

2. Open the filter cover.
3. Rotate the wheel to reach the filter with the requested number.
4. To extract the old filter, unscrew the filter locking ring with a best fitting flat
screwdriver.
5. Replace the filter and the rubber O-ring without mounting any gray quartz
filter.
6. Fix the locking ring.
7. Close the filter cover.
8. In TE-OS-F4-F4 "read all filters" check the filter equalization.
9. If the A/D reading of the new filter is higher than the others, you must open
again the filter and add a gray quartz filter.

SERVICING 107
10. If the A/D reading of the new filter is lower than the others, you must check if
you can increase the gain of the optical preamplifier (if no readings are high-
er than 60,000). If not, you can leave it at its low level if its reading is higher
than 36,000. Else you must add gray filter(s) to the filters with the highest
readings and then increase the optical preamplifier gain again.
Cuvette 0: Position to get filter reading value.

Cuvette 32: Position for screwdriver holes alignment to adjust gain and offset.
- Select the DARK position on the filter wheel with the '0' key and cuvette 32
with the F2 key. Adjust the optical preamplifier offset in the range 500 to
1,000.
- Select cuvette 0 with TE-OS-F4-F2 Select Cuvette. One by one, adjust all the
mounted filters.
- Adjust the readings with gray filters assembled together with the interfe-
rence filters.
- Adjust the optical preamplifier gain selecting cuvette 32, then go back to
cuvette 0.
The order of the filter assembly has to be gray filter, O-ring, interference filter,
locking ring.
The reading of all the filters must be in the range 40,000 to 60,000. Try to reach
50,000 ± 5,000 units.
Execute TE-OS-F4-F5-1 Autozero to verify the final readings (they can be slightly
different from those obtained from the immediate reading) and eventually re-
peat the matching.
6.7 VACUUM PUMP REPLACEMENT
The two vacuum pumps are electrically connected in parallel on the same out-
put signal.
FIGURE 6-21
Vacuum pumps
108
The vacuum pump on the left is aspirating from the wash station aspiration
needles.
The vacuum pump on the right is aspirating from the wash station cleaning noz-
zle.
The outputs of the vacuum pumps are connected to a Y nipple (E) via the hydrau-
lics panel manifold (C) to the output for the waste tank (D).
FIGURE 6-22
Vacuum pumps connections
6.8 VACUUM PUMP MEMBRANE VALVES REPLACEMENT
Unscrew the pump head.
FIGURE 6-23
Vacuum pumps head unscrewed
Open the upper part of the pump head
FIGURE 6-24
Vacuum pumps head opened
The two rubber membrane valves are inserted in two pins and surrounded by
rubber O-rings.
Take off the two membrane valves.

SERVICING 109
Center the two new membrane valves on the two pins and carefully position
them.
Ensure the right position of the two rubber O-rings.
FIGURE 6-25
Vacuum pump membrane valves
Close the pump head and fix it tight by the four screws.
6.9 PERISTALTIC PUMP HEAD REPLACEMENT
Open the side cover of the hydraulic unit placed on the left side of the instru-
ment.
Find which pump you want to replace.
Extract the two tubes of the pump head from the barbed fittings.
Extract the pump head clenching the two clips placed on the two sides of the
pump head.
FIGURE 6-26
Peristaltic pump head replace-
ment
Push the new pump head down until you hear a click and reconnect the two
tubes.
Don't forget to prime the tubing to avoid any trapped air bubbles.
6.10 DILUTER REPLACEMENT
Disconnect the motor connector (MDS1 or MDS2) and the limit switch connec-
tor (SDS1 or SDS2) and take them off the hydraulic panel frame.
110
FIGURE 6-27
Electrical connections of the
diluter
Disconnect the two hydraulic fittings (red marked).
FIGURE 6-28
Hydraulic and mechanic connec-
tions of the diluter
! The bottom left screw is hid-

den by the tube fixation.
Unscrew on the hydraulic panel the old diluter with the four screws (green mar-
ked) at the base of the diluter head.
Place the new diluter and fix it with the four screws.
Connect the two hydraulic fittings.
Insert the new MDS1 or MDS2 and SDS1 or SDS2 connectors on the hydraulic
panel frame and connect them to the wiring.
Go to the TE-OS-F7 Edit Parameters menu.
It is possible to set two options:

- Set parameter (068) DSTYPE to 3 (default setting) to run the diluter at the
maximum speed of 1,520 steps/s.
- Set parameter (068) DSTYPE to 4 to run the diluter at a bit slower speed of
1,400 steps/s.
The parameter (068) DSTYPE is normally set to 3 (default setting). If, due to
hydraulic narrowings, the diluter is not working properly, set parameter (068)
DSTYPE to 4 and the parameter (133) DSVELD to 1,520 or 1,400 to have higher
torque margins.

SERVICING 111
The system parameters whose values are constrained are:

(069) DSOFFS: 0
(070) DSTROK: 368
(071) DSTEPS: 5,200
(078) DSGAPH: -2
(133) DSVELD: 1,400 to 1,800
(134) DSVELA: 1,600
(137) DSVELZ: 1,200
These values are automatically updated on exit of the TE-OS-F7 Edit Parameters
procedure, after having changed the (068) DSTYPE parameter.
On exit, press the F2 key to accept the modifications.
6.11 DILUTER SEALING GASKET REPLACEMENT
The sealing gasket must be replaced before it doesn't seal anymore and there is
a water leakage from the back of the head or from the optical switch opening.
The replacement is also part of the 12 months preventive maintenance.
Remove the system solution connector (blue) to avoid to completely emptying
the tubes.
Unscrew the diluter head with an Allen key and extract the head.
Remove the old sealing gasket.
Place the new sealing gasket, oriented with the orange part on the inner side.
Insert gently the head on the piston, avoiding to damage the new gasket.
Tighten gently the two screws.
Reconnect the system solution connector.
Execute a filling of the water tubes.
FIGURE 6-29
Diluter sealing gasket replace-
ment
112
6.12 SAMPLING ARM REPLACEMENT

FIGURE 6-30
Sampling arm replacement
- Disconnect the wiring connectors and the blue needle tube fitting.
- Unscrew the two screws on the two lower pillars.
- Unscrew the two screws on the two upper pillars. One of the two screws will
remain trapped under the reaction rotor incubator, but the sampling arm
can be anyway disengaged. This screw is the last to be unscrewed during the
disassembling and the first to be screwed during the reassembling.
- Replace the sampling arm, tighten first the two screws of the upper pillars,
then the two screws of the lower pillars, connect the electrical connectors
and the blue needle tube fitting.
- Go to the TE-OS-F2 Mechanical Calibrations menu and adjust all the quotes
related to the sampling needle positions.

SERVICING 113
6.13 LEVEL SENSOR WIRING REPLACEMENT
Refer to the sampling arm replacement procedure for the disassembly and re-
assembly of the arm.
Insert the following wirings from bottom after removing the old ones:
FIGURE 6-31
Sampling arm SN optical limit
switch wiring (16890/113-1)
FIGURE 6-32
Sampling arm level sensor board
wiring (16890/112-1)
FIGURE 6-33
Small motor wiring (16890/201-
1)
Insert the TEFLON tube from bottom after removing the old one:
FIGURE 6-34
TEFLON tube with two fittings
(16890/117-1)
114
Adjust the lengths of the wirings and the tube:
FIGURE 6-35
Lengths of wirings and TEFLON
tube
Legend: Length from A to:

A Shaft with or without black bearing
B Limit switch wiring B ± 27.5 cm
C Sampling needle level sensor board wiring C ± 29 cm
D Small motor wiring D ± 27.5 cm
E TEFLON tube with two fittings E ± 11 cm
FIGURE 6-36
Insert the connectors in the ap-
propriate sockets
FIGURE 6-37
Add the previously cut cable
clamp

SERVICING 115
FIGURE 6-38
Fix the wirings with the clamp
FIGURE 6-39
Sampling arm positioning
Since the new wirings are internally twisted, it is necessary to turn the sampling arm
(top view) counter-clockwise until mechanical blocking. This is to avoid further twi-
sting of the wirings and must be done before proceeding with the central part as-
sembly.
FIGURE 6-40
Pass all wirings through the hole
! Ensure that all wirings will be

free moveable after assem-
bling.
FIGURE 6-41
Assemble the small motor wiring
! Pass the motor cable below

the motor first and then
through the hole.
116
FIGURE 6-42
Routing and connecting
! Swapping/short-circuiting
the connectors can create se-
rious damages.
Legend:
A Small motor wiring
B Small motor
C Limit switch wiring
D Limit switch
FIGURE 6-43
Central part wiring
Fix the limit switch with the upper tie-wrap on the metal support.
Also fix the wirings and the TEFLON tube with the lower tie-wrap on the metal
support. Place the tie-wrap on the rubber coating part overlapping the wirings
or at the blue coating.

SERVICING 117
FIGURE 6-44
Correct lengths
The wiring is composed of six ultra-flexible wires. They are supplied – on the
level sensor board side – already crimped but not yet inserted in the MOLEX 8
pins connector case.
The wires must be passed through the rectangular duct without the connector
case.
They must be routed carefully and inserted into the MOLEX connector. Swap-
ping the wires can damage the boards. Short-circuiting the wires will also da-
mage the boards.
FIGURE 6-45
MOLEX connector
The pins 1 to 3 are used for the SN level sensor and the pins 6 to 8 are used for
the vertical shock detection.
MOLEX MX254 8 pins F MINIFIT 8 pins 4x2 M

1 Green-black 1 Ground
2 Yellow 2 Level out
3 Red-brown 3 24 VCC
4 - 4 -
5 - 5 -
6 Red 6 24 VCC
7 White 7 Shock out
8 Black 8 Ground
The pin numbers of the MINIFIT connector are printed on the back side as follow-
ing:
118
6.14 EXTERNAL TANKS FLOATS REPLACEMENT
Check if the float of an external tank is out of work:
Recommended:
Go to the TE-OS-F5 Washings menu. The status of the floats is displayed in the
upper right corner of the screen.
Normal status:
Water empty / waste full status:
Not recommended:
Go to the TE-OS-F1-F5 Display Inputs menu. The status of the floats is displayed
in a different way in the upper right corner of the screen.
Normal status:
Water empty / waste full status:
! Each float contains a toroidal

magnet in one side. The ma-
gnet must be mounted in the op-
Extract the float from the tank and rotate it upside up and down slowly (5 se-
conds). You must see the displayed status of the float toggle on the screen. If it
is not toggling, it must be replaced.
posite position when it belongs to The contact of each float is closed in the normal status. This means that the
a water tank (normally open) or water tanks are closed in the up (full) position and the waste tanks are closed in
to a waste tank (normally closed). the lower (empty) position.
Refer to "6.15. Inversion of the To replace the wiring, it is necessary to employ the specific 16890/236 "Trim-
float contact". Trio/Mate-n-lok pins extraction tool" to extract the two pins of its cable from
the connector.
Open the connector shield, insert the extractor into the pin to close its harpoons
and pull the wire gently out.
Then insert the replacement pin from the back of the connector and push it until
the harpoons click. Pull the wire gently to verify that it doesn't come out.

SERVICING 119
6.15 INVERSION OF THE FLOAT CONTACT
Each float contains a toroidal magnet in one side of the white body. The magnet
has a specific position when it belongs to a water tank (normally open) and must
be mounted in the opposite way when it belongs to a waste tanks (normally
closed).
Remove the lock washer
FIGURE 6-46
Lock washer reassembly
Remove the white body

Turn the white body upside down
FIGURE 6-47
Float body inversion
Reassemble the white body

Reassemble the lock washer
6.16 OPTICAL PREAMPLIFIER REPLACEMENT
Disassemble the needles group of the wash station (two screws A and B).
FIGURE 6-48
Wash station needle group
replacement
120
Disassemble the black reaction rotor cover (three screws D, E and F) and remove
it.
FIGURE 6-49
Reaction rotor cover replacement
FIGURE 6-50
Unscrew the reaction carousel (six screws A, B, C, D, E and F) and remove it.
Reaction carousel replacement
Rotate the reaction rotor support until the notch is positioned over the optical
preamplifier cover, then unscrew the optical preamplifier cover (2 screws) from
the incubator.
FIGURE 6-51
Positioning of the optical pream-
plifier cover
Push up the optical preamplifier cable (red wires with black sleeve) with the
hand in such a way that the optical preamplifier board is lifted up out of the in-
cubator.
FIGURE 6-52
Pushing up the optical preampli-
fier cable

SERVICING 121
Unscrew the preamplifier board.
FIGURE 6-53
Optical preamplifier board disas-
sembly
Disconnect the cable from the board.
FIGURE 6-54
Optical preamplifier cable disas-
sembly
Replace the optical preamplifier board and reassemble everything.

Adjust eventually gain and offset (Refer to "5.2. Optical gain and offset".)
6.17 ADDING A NEW OPTICAL INTERFERENCE FILTER
On the filter wheel, position 9 is free to be used for special applications.

To install a special filter, you have to simply mount the filter in position 9.
Then you have to configure it in the system parameters. Set parameter (328)
FILTR9 to the wavelength value of the filter.
If you are in the HI software, you have to exit and restart HI to acknowledge the
new filter.
If a method employs a filter not on board, at the moment of scheduling HI will
issue an error on the programmed test.
122

FIRMWARE UPDATING 123
7 FIRMWARE UPDATING
7.1 Download/upload of the system parameters
It is recommended to download (i. e. back-up) the system parameters, before

doing the firmware upgrade.
In case the CPU board has to be replaced it is mandatory because the flash me-
mory of the new board is empty. Then you can easily retrieve the system para-
meters after CPU board replacement.
The software tool for the download/upload is the 'ParametersManager.exe' in
the upgrade folder's sub-folder 'ParametersManager'.
7.1.1 DOWNLOAD
1. Start the ParametersManager.

2. Select the baudrate 115200.
3. Type 'HS300SR' in the 'Oem' field and the serial number of the analyzer in
the 'SN' field.
4. Select from 'Instruments' in 'Download' the right COM port. (It is the COM
port for the communication between the analyzer and the PC.)
5. The download runs in the right frame. When finished, it shows the number
of downloaded parameters in the 'Param. Number' field and a confirmation
in the status line.
FIGURE 7-1
ParametersManager – download
finished
124
6. Go to 'File' and select 'Save' or 'Save as…' to open the Windows dialog menu
fo storing a file. You can either store the parameters as an ordinary text file
with the extension '.PAL' or as a tagged file with the extension '.XML'.
FIGURE 7-2
PAL type download file example
FIGURE 7-3
XML type download file example
7.1.2 UPLOAD
The upload of the saved system parameters works in the opposite way.
1. Start the ParametersManager.

2. Go to 'File' and select 'Load' to open the Windows dialog menu fo loading a
file. Select the appropriate PAL or XML type download file. When finished,
the message 'Loading from <file type> … Successfully loaded' appears in the
right frame.
3. Select the baudrate 115200.
4. Select from 'Instruments' in 'Upoad' the right COM port. (It is the COM port
for the communication between the analyzer and the PC.)
5. Following warning appears which has to be confirmed:
FIGURE 7-4
Upload overwrite warning
6. When finished, the message 'Uploaded <XXX> parameters' appears in the

right frame.

7.2 Special USB cable
On the HS300SR there is a programming USB COM port.

A special USB cable must be used for the programming of the firmware on the
flash memory of the CPU board.
FIGURE 7-5
Special USB cable
7.3 CPU board version 2.5.1

FIGURE 7-6
CPU board 2.5.1
7.4 Upgrade procedure
1. Find out, if your PC is a 32 bit system or a 64 bit system. E. g. in Windows 7:

Click on Windows 'Start' icon. Right-click on 'Computer'. Select 'Properties'.
Find the information in section 'System', 'System type'.
2. In the upgrade folder, open the sub-folder 'ArduinoDueDrivers_2016'.
3. For a 32 bit system, run 'dpinst-x86.exe'.
4. For a 64 bit system, run 'dpinst-amd64.exe'.
5. Follow the presets in step 3 or 4. Finally 4 drivers will be installed.
126
7.4.1 EDIF TERMINAL RELEASE 2.3.5
1. Run the EDIF Terminal rel. 2.3.5.

2. Select the communication COM port and the 115200 baud rate.
3. Click the "Connect" button.
4. Check the communication with the CPU. Remember the communication
COM port number.
5. Exit the Terminal program.
7.4.2 HUMASTAR 300SR
1. Open the front cover of the HS300SR and remove the boards cover.
2. Connect the special USB cable to the CPU (connector on the left marked
"USB" on the picture).
3. Connect the special USB cable to an USB port of the computer.
7.4.3 UPGRADE
1. Open the DuoUpdate folder.

2. Run the LOAD.BAT program.
3. The List of the current COMs with, at least, one available COM port is shown.
4. To clear the old firmware:
- Short circuit the two pins marked as "U2" (at the top left of the CPU chip
on the picture).
- While the two pins are shorted, press the "RESET" button (at the top right
of the CPU chip on the picture).
- The two LEDs (blue and red) at the top of the CPU will go off.
- Open the short circuit of the two pins marked as "U2" again.
5. Type C<Enter> to select the programming COM port.
6. Type COMxx<Enter> to select the programming USB port. (xx is the number
of the COM port).
7. Type P<enter> to start the flash programming.
8. After 3 seconds the flash writing starts followed by the flash verifying.
9. At the end of the verifying, press the "RESET" button (at the top right of the
CPU chip on the picture). After a few seconds, the red and blue leds will
switch on.
10. Close the flash program and disconnect the special USB cable.

7.4.4 EDIF TERMINAL RELEASE 2.3.5
1. Run the EDIF Terminal rel. 2.3.5.

2. Select the communication COM port and the 115200 baud rate.
3. Click the "Connect" button.
4. Press <Enter> twice.
5. If the release (version) of the firmware is changed, you have to run the Firm-
ware Update procedure (as for HS100/200). When prompted, select the op-
tion 1 Load+check.
6. Exit the Terminal program.
7. Run the HI software.
128

HI software InstallatIon 129
8 HI SOFTWARE INSTALLATION
8.1 Settings
The HI software is a Windows.Net application and runs under Microsoft Frame-

work 4.6.2. It also requests some more Microsoft applications (report viewer and
database management). All these Microsoft tools are already included in the
installation media and are installed automatically before the HI software.
The program runs with almost any last generation CPU‘s. It is suggested to have
a multithread CPU and a graphic adapter. For slow cheap CPU‘s it can be neces-
sary to disable the fading and fog effects in the HI software settings to reduce
the graphics CPU load.
The software auto-scales on the computer screen. The minimum required reso-
lution is horizontal 1280 pixel and vertical 768 pixel.
8.2 HI software updating
8.2.1 LOG ON
To install the software you need to log on your computer as Windows admini-
strator.
8.2.2 CREATE A BACKUP COPY OF THE DATA FOLDER
The software changes the structure of the patient‘s database.

It is recommended to back-up the Hi data folder. If you want to return back to
the old release you have to replace the newly modified "Hi" folder by the old
backup data folder. See "8.3 Hi data folder" to locate the "Hi" data folder.
8.2.3 INSTALL THE NEW SOFTWARE
If the new software has been downloaded:

- Unzip the installation software file (if it is compressed).
- Execute the "HiSetup.exe" program.
130
If the new software is on an USB stick, just insert the stick and the installation
will normally start automatically. If not, execute the "HiSetup.exe" program.
8.2.4 INSTALLATION STEPS
1. Only if the old installation has not been correctly uninstalled, a new window
will ask you to remove the old installation. In this case, confirm that the old
installation must be removed, then press the "CLOSE" button and restart the
installation.
2. For the "FTDI CDM - USB-RS232" drivers installation, press "INSTALL". A DOS
command window will open during this installation.
3. MICROSOFT LICENSE AGREEMENT window. (If the Microsoft Framework
4.6.2 tools are already present in your computer, this step can be skipped by
the installer). Select the "I agree" option 3 times, first time for Microsoft Fra-
mework, second time for Microsoft Report Viewer. In this case, the system
will ask you to reboot the computer. Accept and wait the computer restarts
and enters again in the installer.
4. HI (HUMAN INTERFACE) LICENSE AGREEMENT window. Select the "I agree"
option and press the "NEXT" button.
5. Select the "COMPLETE INSTALLATION" option.
6. CONFIRM INSTALLATION window. Press "NEXT" button. The software is in-
stalled in a few seconds.
7. INSTALLATION COMPLETE window. Press the "CLOSE" button.
8. The installer creates a data folder named "Hi" with only void files. If you want
to keep your old data, you have to copy the old "Hi" data folder. Follow the
steps described in the next chapter. Else refer to your distributor for your de-
fault data folder. In the installation USB stick a reference default data folder
is supplied.
9. An "hi" icon is now on the desktop to start the program.
FIGURE 8-1
hi software logo and icon
10. The first time you start the program, you must select the COM port that will
be used for the communication with the instrument.
11. Since the internal method structure could have been changed, log on as Ad-
ministrator or Installer and execute the "METHODS UPLOAD" to synchronize
the instrument with the PC.

HI software InstallatIon 131
8.3 Hi data folder
The software installer initializes a void data folder.

You can copy your own data folder or a default data folder, replacing the void
folder created by the installer.
The path for Windows 10 or Windows 7 is "C:\ProgramData\Hi".
To maintain the application data from an old installation, copy the old "Hi" fol-
! The ProgramData folder and
all subfolders are hidden fol-
ders by default. To view them,
der and paste it in the following folder for Windows 10 or Windows 7: "C:\Pro- you must go to "Windows Control
gramData". Panel > Folder Options > View"
Once copied, rename your new data folder to "Hi" if the old folder was renamed. and set the "View Hidden Files"
option.
To avoid the risk of data loss, it is anyway safe to backup periodically the "HI"
data folder.
DEFAULT METHODS: In the installation USB stick there is a set of files that will
permit the automatic generation of default data sets at the first run of the soft-
ware.
8.4 Compatibility with Windows 7, 8, 8.1, 10
When the Microsoft Framework 4.6.2 is installed, there is full compatibility with
Windows 7, 8, 8.1, and 10.
Note that this version of the framework is not compatible with Windows XP or
previous versions of Windows.
8.5 Device drivers
Check that the following drivers have been loaded (normally installed by the HI
software installation):
- Microsoft.net Framework 4.6.2
- Microsoft Report Viewer 2010 redistributable
- FTDI CDM Driver package 17/02/ 2009 24.16 (this driver reinstalls at every
installation).
132

TROUBLESHOOTING 133
9 TROUBLESHOOTING
9.1 The analyzer does not connect
9.1.1 NOT CONNECTED
FIGURE 9-1
"Not connected" display and
"Connect" icon
Press the "Connect" icon. If it doesn't connect:
1. Check if power is ON (red lamp on the main switch and orange lamp on the
reagents cooling switch).
2. Switch the instrument OFF, wait five seconds, and switch ON again. At po-
wer-on, the instrument must shake the sample plate three times to show
it is working. If it doesn't shake or shake only twice, contact the service. No
shake means that the CPU or the power board 0 are not working. Try again
to connect. If it doesn't connect, continue with step 3.
3. Verify if the USB cable between the computer and the instrument is correctly
inserted. Try again to connect. If it doesn't connect, continue with step 4.
4. Verify the USB port. Exit from the software. Enter the Windows Control Panel
> System > Device Manager. Unplug and insert again the USB cable from the
instrument. Verify that the computer correctly detects the COM port (Ports
COM & LPT > USB serial port (COMxx)). If not, check the USB cable and the
USB port on the computer. Replace the USB-to-RS232 converter to check if
the problem is in the internal converter board. If the USB port is detected
correctly by the PC, note which is the detected COM port and continue with
step 5.
5. Check the internal serial port. Start the EdifTerminal_2_3_5.exe program. Se-
lect in the upper left corner the COM port detected in the previous step. Click at
the center of the window to focus on it. Shut the instrument OFF and then ON.
Wait for the three shakings of the sample plate and for waste pumps movement.
134
The instrument motors are OFF and is displayed on the screen as follow:
Press ENTER two times. The instrument motors go ON (hold) and a must ap-
pear on the screen, after the characters already appeared, as follow:
If #CDEF Ready<NAK> doesn't appear, the instrument is not transmitting on

its internal RS232 output. If it doesn't answer to <Enter> (the motors remain
OFF), the instrument is not receiving on its internal RS232 input. It is then ne-
cessary to check the internal USB-to-RS232 adapter, the internal serial cable
and the CPU board.
9.1.2 NOTES ON THE USB-TO-RS232 ADAPTER
! Do not unplug the USB cable

when the software or the
EdifTerminal is running, else it
Every USB controller has its own internal serial number. This means that, for the
PC, every USB controller corresponds to a different COM serial port. Once it has
been detected for the first time, the same COM port number is assigned to that
will not reconnect. The software USB controller.
will not find anymore the USB For this reason, if you connect two instruments to the same PC, every instru-
port and it could crash. ment will have its own COM port assigned.
EdifTerminal 2_3_5.exe on the upper left corner lists all the detected COM ports
and allows checking the correct detection of the USB adapter.
9.2 Optical readings have bad repeatability
There can be several reasons that can cause bad precision in readings:
1. Noise on the optical signal.

2. Imprecision in the reaction rotor positioning.
3. Imprecision in the filter wheel positioning.
4. Dispensation problems.
5. Incorrect vertical positioning of the needle dispensation in OP.

TROUBLESHOOTING 135
9.2.1 NOISE ON THE OPTICAL SIGNAL
It can be checked in the TE-OS-F4 Optical Reading Test menu.

Selecting filter 0 (dark), the signal must be stable. The maximum range of oscil-
lation is 1 unit.
Selecting filter 1 and tube 0, the signal must be in the range 40,000 to 60,000
and the maximum oscillation range must be lower than 20 units.
If not, check in this order:
1. Spray a contact cleaner on all the lamp and optical contacts, in the front and
in the back.
2. The lamp is possibly defective or close to end-of-life.
3. The optical signal cable from the optical preamplifier to the power board 0.
4. The optical preamplifier.
See next chapter "9.3. Autozero has bad repeatability, unstable optical signal"
for more detailed troubleshooting.
9.2.2 IMPRECISION IN THE REACTION ROTOR POSITIONING
It can be checked with TE-OS-F3-F4 OP+FS home function. If the positioning is

unstable, the belt must be tightened correctly. (Refer to "5.4.2. OP belt tighte-
ning".)
9.2.3 IMPRECISION IN THE FILTER WHEEL POSITIONING
It can be checked with TE-OS-F3-F4 OP+FS home function. If the positioning is

unstable, the belt must be tightened correctly. (Refer to "5.3.3. FS belt adjust-
ment".)
The imprecision in a single filter can be caused by a loose filter locking ring.
9.2.4 DISPENSATION PROBLEMS
Check if the silicone tube is well inserted in the pinch valve and that it is not
worn.
Check that all the pipetting TEFLON fittings are well tight.
136
Check the diluter gasket, eventually replace it.

Check the diluter mechanics by the TE-OS-F3-F7 DS1/DS2 function.
Check if the sampling needle is clogged.
9.2.5 INCORRECT VERTICAL POSITIONING OF THE NEEDLE
Incorrect vertical positioning during the dispensation and the mixing in the re-
action rotor (053) SN1DS2 and (369) SN2DS2 quote.
Execute the TE-OS-F5-F5-1 Test Pumps function.
At the end of the test the program will eventually suggest to update the (053)
SN1DS2 and/or (369) SN2DS2 mixing quote.
9.3 Autozero has bad repeatability, unstable optical signal
Go to TE-OS-F4 Optical Reading Test menu, wait that the instrument is well
warmed-up and do the following tests:
1. Select filter 0 (dARK) and disconnect the optical cable from the Power Board
0.
The A/D signal on the screen must be close to 20 and stable.
If not, there is a problem on the Power Board 0 or on the optical preamplifier/
multiplexer.
2. Re-connect the optical cable on the Power Board 0 and disconnect the opti-
cal preamplifier.
The A/D signal must be close to 20 and stable.
If not, there is a problem on the optical cable.
3. Reconnect the optical preamplifier.

The A/D signal must be in the range 50 to 100 mV (500 to 1,000 A/D units)
and stable ±0.4 mV (±4 A/D units).
If not, there is a problem in the optical preamplifier.
4. In the TE-OS-F4 Optical Reading Test menu, select filter 1 (340 nm).
The A/D signal must not oscillate more than 3.0 mV (30 A/D units).
If not, it's possibly a problem of the lamp either the lamp contacts or optical
preamplifier contacts.
Clean all contacts with a contact cleaner.

TROUBLESHOOTING 137
5. Select filter 2 and then again filter 1. Then filter 3 and again filter 1, etc.
Wait 10 seconds. The A/D signal on filter 1 must come back to the original
value within 4 mV (40 A/D units).
If not, there is a problem in the filter wheel positioning.
Check in the TE-OS-F4-F5-2 Autozero Test function the filter wheel offset.
If the filter wheel belt is well tightened and it is not damaged (TE-OS-F3-F4
OP+FS Home function), it can happen that the belt doesn't look damaged
but it doesn't work well anyway. If you are in doubt, replace the filter wheel
belt anyway.
9.4 Reaction rotor temperature is uncontrolled
Involved devices:
- Reaction rotor temperature sensor and wiring.

- Reaction rotor etched coil heating resistance and wiring.
- Reaction rotor thermostat.
- Power Board 0.
Go to TE-OS-F1 Hardware Test menu.

Press F5 for continuous inputs display.
Observe the inputs window in the upper right corner:
1. The displayed temperature is higher than 500 (tenths of °Celsius), 50 °Celsius

and PWM R = 0.
- The reaction rotor is at room temperature: the temperature sensor is bro-
ken or disconnected.
- The reaction rotor is really at 50°C. The Power Board 0 could be broken or
the temperature sensor could be damaged.
2. The displayed temperature is correctly 38 ±0.2°C, but the reaction rotor tem-
perature is wrong.
The temperature measurement is not adjusted. Increase or decrease the pa-
rameter (285) OPTCAL.(10 units are 1 °Celsius).
3. The displayed temperature and the reaction rotor are at room temperature
and PWM R = 70.
The heating is not working. Check the voltage on the resistance connector:
138
- If the voltage on the resistance is between 16 V and 24 V, the resistance

is broken.
- The voltage on the resistance is close to zero. Check the voltage on the
Power Board 0 connector J14, pins 5 and 6.
- The voltage on the Power Board 0 connector is between 16 V and 24 V.
Check the wiring and the thermostat.
- The voltage on the board connector is close to zero. The Power Board 0
is broken.
4. The displayed temperature is close to zero and the reaction rotor tempera-
ture is high:
The Power Board 0 is not working or the temperature sensor is short-circui-
ted.
9.5 Reaction cuvettes become dirty and excluded quickly
9.5.1 FIRST CHECKS
- Check if the specified system solution is used.

- It is possible that some reagents can create problems in the washing. Verify
that all the employed reagents are well tested for the wash purposes.
- Check if all the reaction volumes are limited to 310 μl?
- Verify that the user never shuts the instrument off before the washings have
been completed (in run or in shut-down).
9.5.2 CHECK THE REACTION CUVETTES
It is important to check and find out in which way the cuvettes are dirty, because
it is very important to understand the cause.
The absorbance threshold for the cuvette to be considered clean is 120 mAbs at
340 nm. Typically the absorbance for a new cuvette at 340 nm is lower than 60
mAbs, and for the other wavelengths is lower than 35 mAbs.
Check the spectrum of the excluded cuvettes in HI – Maintenance – Special –

Cuvettes.
The deposits that absorb at 340 nm cannot be seen by the human eye, so the
cuvette seems clean.

TROUBLESHOOTING 139
If the spectrum is very high at 340 nm and low at the other wavelengths, the
cuvette looks clean to the eye, but it is anyway excluded.
High absorbances on all the wavelengths are typical for scratches or uncolored
(white/grey) deposits.
9.5.3 CHECK THE CAUSE OF HIGH ABSORBANCES
- If there are internal deposits, the washing is poor. If the deposit is coloured,
you can try to understand which reagents cannot be washed.
- If there are internal vertical scratches, the wash needle(s) touches/touch the
inner side of the reaction cuvette.
- If there are external horizontal scratches, the reaction cuvette touches so-
mewhere and is scratched during the rotation.
- If there are external "clouds", the vacuum pump is not working well or the
operator made some mistake and water overflowed outside. The "cloud" is
the solid deposit that remained on the outer side of the cuvettes after the
water is evaporated. In this case the two external sides of the cuvettes can
be cleaned with a soft cloth.
- If the area is highly humid, the two external optical faces of the cuvettes can
become dirty after some months. Take off the cuvettes rotor (Refer to "6.4.
Reaction rotor replacement ") and pass a soft optical cloth on the two optical
faces of the 80 cuvettes mounted on the rotor.
9.5.4 EXECUTE THE PUMPS TEST
Put a 5 ml tube filled with tap water in sample position 1.

Run the TE-OS-F5-F5-1 Test Pumps test.
Verify that, in the aspiration test, the aspiration flow is higher than 1,500 μl/s
and that the final result is Asp. PASS (not Asp. FAIL).
The Wash Station dispensation must have values higher than 350 μl.
If the Aspiration test failed, the membrane valves of the vacuum pump or the
entire pump must be replaced.
If the Wash Station Dispensation is low, the peristaltic pumps timers must be
increased. This can be done manually, increasing the (160) DIWSH1 to (164) DI-
WSH5 parameters in the following way:
140
Example:
- Pump dispensation = 300
- Old value of DIWSH1 = 50
- New value of DIWSH1 = 50 x 400 / 300 = 67
The DIWSHx parameters range is 45 to 80. If the parameter must be set higher
than 80, the pumps head must be replaced.
The correction of the pump timers can also be made automatically executing
the TE-OS-F5-F5-5 Adjust Pumps procedure.
9.5.5 EXECUTE CUVETTES SPECIAL WASH
To eliminate protein and lipid deposits in the reaction cuvettes, put a reagent
bottle with 26 ml of NaOH solution at 4% in reagent position 1 and start the
Special Wash Procedure (HI – Maintenance – Special cuvette wash).
At the end, a Startup procedure is executed.
To eliminate salt deposits in the reaction cuvettes, put a reagent bottle with 26
ml of HCl solution at 5% in reagent position 1 and start the Special Wash Proce-
dure (HI – Maintenance – Special cuvette wash).
At the end, a Startup procedure is executed.
9.5.6 INSTRUMENT DISPLAYS YELLOW RATHER THAN GREEN CUVETTES
1. Execute the TE-OS-F4-F6- 2 Fine Offset procedure to check if the OP offset

has changed or if there is a strange behavior. If it were so, save the new value
and perform the startup again.
2. Check if there has been a liquid overflow from the wash station. The water
goes inside the incubator, evaporates and condenses on the walls of the cu-
vettes, increasing suddenly the O.D. of all the cuvettes.
When the condensation vanishes, the O.D. returns back to the right levels.
3. If the problem persists, verify if the cuvettes O.D. is really bad. Go to the TE-
OS-F4 Optical Reading Test menu in and follow the instructions below:

TROUBLESHOOTING 141
- Select the filter number1

- Press F2 and select cuvette 0 (which corresponds to reading in air)
- Press F1 to set the reference
- Press F2 and select one of the theoretically dirty cuvettes.
Verify the immediate O.D. A clean cuvette without water has about 130
mAbs. If the obtained O.D. is similar, it means that the cuvettes are good
(green) but the instrument detected them as dirty (yellow) because the
OP rotor is moving incorrectly.
Try to reduce the (125) OPVEL and the (126) OPVELZ parameters or else
try to increase or decrease manually the (022) OPOFRD parameter by 1
unit.
Please note:
The difference value between the filter 340 nm in real time and the filter 340 nm
on the Autozero must be lower than 700 units. If this difference is higher, there
is some discrepancy on the optical path (lamp, fine offset, Adjust FS offset).
9.6 A droplet forms on the needle tip
9.6.1 AT THE END OF THE NEEDLE TIP
If the droplet is formed at the end of the needle tip, there can be several causes.
Most common causes are:
- The silicon tubes in the pinch valve could be hardened.

Remove them and try to extend a little bit by hand in order to relax them.
Put the tubes back into the pinch valve.
- The silicone tubes inside the pinch valve are damaged, not correctly inserted
or too long out of the pinch valve toward the diluter (max. 1 cm).
Check the silicone tubes and eventually replace it.
- There are air bubbles in the diluter or the needle tubing.
- There can be a leakage in the fitting under the level sensor board or between
the TEFLON tube and the sampling needle.
- There can be a narrowing somewhere in the needle tubing so that the tubes
blow up and the water flow is delayed.
- The needle vertical positions (045) SN1WSH abd (364) SN2WSH in the wash
well are not well calibrated and the needle tip do not pause on the white
nipple 1 mm inside the water drop to let the droplets be captured.
142
9.6.2 ON THE SIDE OF THE NEEDLE
If the drop hangs on a higher position on the side of the needle, it has probably
been left there by the jet of the external wash nozzle.
Clean the needle with alcohol to ease the drop fall.
You can reduce the water jet speed with the parameter (188)°EXWELD, so that
the jet hits the needle in a descending parabola, allowing a better leaching of
the droplet. Try to reduce the (188)°EXWELD parameter by 5 or 10 units.
If the external wash nozzle splashes the needle at the end of the internal needle
wash, the delay for the valve commutation (186)°ASWELD is too low (increase it
by 10-30 units) or the Y tubing connected to the well dispensation pump, placed
on the back side of the hydraulic panel, is too resilient and balloons. Eventually
replace the affected silicon tube.
9.7 Aberrant or null results and reagent bottles wrongly detected

full (green)
It happens more or less frequently that some reagent bottles are detected as
completely full (green color) even if they are not.
At the same time it happens that the needle sometimes fails to pipet the sample
or reagent 2 (the results are null) or fails to pipet the reagent 1 (the results are
aberrant).
Most probably the sensor level wiring of the sampling arm is broken.
Open the sampling arm cover and check the level sensor LED during the execu-
tion.
If the red LED blinks when the arm is still in a higher position, before having
detected the liquid level, the level sensor board wiring is broken and must be
replaced.
Refer to "6.13. Level sensor wiring replacement".

TROUBLESHOOTING 143
9.8 "Needle shock detector is stuck" error message
The "Needle shock detector is stuck" message is issued when the needle is in an
upper position and begins to move downwards.
If a shock signal is detected before starting the movement, the shock sensor is
not working properly and the error message is issued.
There can be three causes:
- The needle is bent or the white cap op top of the needle is too tight. In these
cases the needle doesn't slide well and it can remain in the upper "shock"
position.
- The optical sensor of the needle shock is broken. Replace the level sensor
board.
- If the level sensor wiring is broken and the white wire of the shock signal is
open, the system detects this as a shock situation and the alarm is issued.
Replace the level sensor board wiring. (Refer to "6.13. Level sensor wiring re-
placement".)
9.9 Vacuum pump errors
9.9.1 DIFFERENT CAUSES
The vacuum pump error can be generated by several different causes. The first
two causes are hydraulic problems. The last two are simply problems of bad me-
chanical calibration of the sampling needle in the reaction cuvettes.
1. The vacuum pump 1 (connected to the wash station needles) is not working
properly. The most common cause is that the pump has aspirated some dirt
which remains on the input valve disk disturbing the aspiration. Alterna-
tively the valve disks could be worn and must be replaced (Refer to "6.8. Va-
cuum pump membrane valves replacement").
2. One (or more) wash station needles are clogged and must be cleaned and
unclogged with a metal wire.
3. The sampling needles rub on the side of the reaction cuvette when it checks
the level (in the OP dispensation position).
144
Go to TE-OS-F2 Mechanical Calibration menu, row 19 and adjust the needles

dispensing position in such a way that the needles don't touch the wall of
the cuvette.
4. The sampling needle OP dispensation position is too low, i. e. (053) SN1DS2

and (369) SN2DS2 are too high. When the needle checks the level, it touches
the bottom of the reaction cuvette, wrongly triggers the level sensor and
detects consequently a water level even if the cuvette has been emptied.
Go to the TE-OS-F5 Pumps Tests menu and select option1 "Test pumps" to
automatically adjust the needle vertical position.
9.9.2 THE TE-OS-F5-F5-1 TEST PUMPS PROCEDURE
Executing the Test Pumps procedure in TE-OS-F5-F5 Pumps Tests menu, we

have the information to detect the type of problem. This test must be executed
when the tubes are already well filled, without air bubbles inside.
At the end of the pumps test, if the (053) SN1DS2 and (369) SN2DS2 quotes are
not to the target this pumps test will allow you to calibrate the dispensation
vertical position (053) SN1DS2 and (369) SN2DS2 (to avoid problem 4). Duri-
ng the needle dispensation, you can check through the dispensation hole if the
needles are rubbing on the side of the reaction cuvette (to avoid problem 3).
It allows also to understand which kind of problem can have the vacuum pump.
If one needle remains with a very high level of liquid, it means that this needle
is clogged (problem 2).
If all the needles are high, the vacuum pump is not working (problem 1).
9.10 Non-repeatable tests troubleshooting
When there are several inconsistent tests we could have three different pro-
blems, mechanical, hydraulics and electronics. It is very important to immedia-
tely understand how to find the solution.

TROUBLESHOOTING 145
FIGURE 9-2
How to troubleshoot
9.10.1 AUTOZERO TEST
Perform the Autozero test (TE-OS-F4-F5-2). Here it is possible to check the lamp,
the optical preamplifier, OP, FS and the complete reading unit.
9.10.2 TEST PUMPS
Perform the Test Pumps procedure (TE-OS-F5-F5-1). Here it is possible to adjust

the calibration of the mixing quote. It is possible to check the dispensation and
aspiration pumps also.
9.10.3 OP+FS HOME
Perform the OP+FS home test (TE-OS-F3-F4). Here it is possible to check the OP
and FS movements. OP and FS are the most important units to achieve good
readings.
9.11 OP calibration error
Instrument reads through the cuvettes more clear than through the gaps bet-
ween the cuvettes.
1. Verify the Fine Offset (TE-OS-F4-F6-1).

146
2. Try to reduce the OP speeds (125) OPVEL and (126) OPVELZ) or try to adjust
the belt tension or modify manually the (022) OPOFRD parameter 1 unit.
The difference values between the 340 nm filter in real time and the 340 nm fil-
ter on Autozero must be lower than 700 units. If this difference is higher means
that there is some discrepancy on the optical path (lamp, fine OP offset, adjust-
ment of the FS offset).
9.12 Invalid autozero
One or more filter values are lower than 5,000 mV or in overflow. You can ex-
tend the range, in emergency case, by decreasing the parameter (352) MINAUZ.
1. Verify in TE-OS-F4 Optical Reading Test menu if the filter values are good in
real time or if the values in real time are also lower.
2. If in TE-OS-F4 Optical Reading Test menu everything is okay, the OP is mo-

ving badly. Try to fix the OP movement (OP speed or adjust the belt tension)
or improve the OP offset by TE-OS-F4-F6-1 Fine Offset procedure or manually
modify the parameter (022) OPOFRD.
3. If in TE-OS-F4 Optical Reading Test menu the autozero value is really lower
than the range, verify the optical preamplifier pins and the lamp pins. If the
problem persists, replace the lamp.
The difference values between the 340 nm filter in real time and the 340 nm fil-
ter on Autozero must be lower than 700 units. If this difference is higher means
that there is some discrepancy on the optical path (lamp, fine OP offset, adjust-
ment of the FS offset).
9.13 Spikes
There are one or more reading points that are very close to zero or in positive or
negative overflow (unusable result).
1. Higher spikes O.D. in overflow (unusable results).

The possible causes are the optical wiring or the filters selection.

TROUBLESHOOTING 147
Check all optical wiring pins and cables, perform the FS tests or try to adjust
the FS belt tension, try to reduce the FS speed.
2. Lower spikes O.D. nearly zero or negative (unusable results).

The possible cause is the OP. Adjust the OP belt tension or reduce the OP
speeds (125) OPVEL and (126) OPVELZ.
3. Small spikes (bad CV%).

The possible causes are air bubbles or some electrical noise.
Perform the Test Pumps procedure in TE-OS-F5-F5 to update the mixing quo-
te and put contact cleaner on all optical pins (preamplifier and lamp).
9.14 Wash station down error
Wash station needles or pipe cleaner could hit on the cuvettes or the wash sta-
tion down limit switch could be broken.
1. If the down limit switch is okay, check the wash station calibration in rows 17
and 18 (TE-OS-F2 Mechanical Calibration).
2. If the calibration is okay, reduce the OP speed or adjust the OP belt tension.
148

Maintenance 149
10 MAINTENANCE
The instrument requires daily, weekly, monthly and special maintenance as de-
scribed in the User's Manual.
In addition a complete maintenance has to be performed once per year.

For a detailed description, please refer to the specific document, provided by
HUMAN together with the maintenance kit.
10.1 Update firmware and software
If the firmware and the software of the instrument are significantly old, proceed
to update the firmware and the HI software. Check the release on the "Info" but-
ton on the lower left corner of the software screen.
FIGURE 10-1
Instrument information
10.2 Cover
Check if the instrument is correctly levelled.

Check if the plexiglas cover shuts correctly the cover detection switch.
10.3 Optical lamp
Check the lamp life. Lamp life ranges between 1,000 and 2,000 hours. If the
lamp has more than 1,000 hours of work and a new maintenance check-up is
not scheduled, you can consider changing the lamp (remember to reset the
lamp timer).
150
10.4 Sampling needle
Check the sampling needle (insulating glass coating, shape).

Check if the needle slides correctly in the needle holder, else take off the holder
and clean it from eventual deposits.
Check if the needle holder is not broken or worn.
Check the shock sensor in TE-OS-F1-F5.
Check if the sampling needle is clogged. Clean it with a 0.5 mm copper wire and
then with special wash solution.
10.5 Wash station needles
Check if the wash station needles are clogged, especially the first long needle
on the right.
Eventually unclog them with a probe or a copper wire. The deposit on the need-
les depends on the type of reagents employed. Clean them with the special
wash solution (basic) to remove protein or lipids deposits. Use an acid solution
to remove salt deposits.
Check if all the needles are not bent and are well centered in the cuvettes (TE-
OS-F2-17).
Check if the dryer needle tip is not worn or broken or rotated.
Replace all the damaged needles.
10.6 Wiring, connectors
Check the lamp connectors (in the back and on the power board) and spray the
contacts with a contact cleaner.
In TE-OS-F4, filter 1 selected, move the lamp wires and connectors. The reading
value must not change for more than a few A/D units. Else, check the lamp wires.
Check the arm wiring: wires can be stressed or, inside the isolation, the copper
could be broken. The continuity of the signal is then precarious. If there is
any sign of wear or breakage, replace the wiring (level sensor board wiring
(16890/112-1), optical limit switch wiring (16890/113-1) and the small motor
wiring (16890/122-1)).

Maintenance 151
10.7 Hydraulic tubes, floats
Replace old tubes, dirty or with algae.

Check if there is air leakage from the hydraulic connectors of the tanks, check
the tubes collars.
Check if there is any sign of liquid leakage on the base and eventually correct
the cause.
Check if the 4 floats work correctly (check the floats in TE-OS-F5).
10.8 Diluter
On the diluter (16890/94), replace the white & orange gasket (16890/95) inside
the diluter head – see "6.11 Diluter sealing gasket replacement").
10.9 Pumps
Run the TE-OS-F5-F5-1 Pumps test and print the report.

Adjust the (053) SN1DS2 quote if requested by the pumps test.
Check the peristaltic pumps life and flow rate.
If the flow rate of any pump is lower than 500 μl/s or if the pump head work
time is higher than 30 to 50 hours, replace the pump's head, reset the pump's
work timer and then execute the pumps adjustment procedure (TE-OS-F5-F5-5).
On the pumps test report (TE-OS-F5-F5-1), check the vacuum pump flow rate.
If lower than 2,000 μl/s, do as follow:
- First check if any aspiration needle (the longer ones) is clogged and eventu-
ally unclog them.
- Else replace the pumps membrane valves (see "6.8 Vacuum pump memb-
rane valves replacement") or the vacuum pumps (see "6.7 Vacuum pump
replacement").
- Else again, check if the hydraulic circuit waste is clogged.
10.10 Reagents cooling
Check the power supply voltage (14.9 to 15.1 Vcc).

Check if the cold plate is correctly cooled (3 Peltier element positions).
If not, check the supply current. It must be in the range 2.3 to 2.6 A.
152
Clean the cold plate, remove eventual mildew.

Check if there is mildew between the warm and the cold plate.
10.11 Sample plate
If necessary, clean it with a sterilizing solution and then rinse abundantly, wipe
and dry.
If it is a 20 + 20 positions sample plate (16890/11), check if the external tubes
clips are well inserted and not damaged.
10.12 Reaction rotor cuvettes
Check on the reaction rotor the cuvettes absorbances. If absorbances are typi-
cally higher than 800, take the reaction rotor off and clean gently the two exter-
nal faces of the cuvettes wiping them with a lens cleaning cloth. Pay attention
to avoid dust deposits on the two faces. After the cleaning, execute a wash of all
the cuvettes (TE-OS-F5-F2, N. of washes: 80).
Replace cuvettes which show an O.D. higher than 1,000 units (= 100 mAbs). See
"6.3 Single reaction cell replacement".
After these two operations it is necessary to execute the startup. There is a fast
version of the startup in the Washings menu.
10.13 Test and adjustment procedures in the TERMINAL SERVICE

menu
The following menus must be called from the TERMINAL SERVICE menu to exe-
cute the test procedures:
10.13.1 F2 MECHANICAL CALIBRATION
Check all the mechanical calibrations, especially the sampling needle dispensa-
tion quote (053) SN1DS2 and (369) SN2DS2, the wash station down quote (115)
WS_ASP, the sampling needle wash in the well drop (045) SN1WSH and (364)
SN2WSH, the sampling needle in the reagent bottles and in the sample tubes
and cups.

Maintenance 153
10.13.2 F3 MECHANICAL CHECK
Verify the belts and the mechanics executing the mechanical check tests, espe-
cially TE-OS-F3-F4.
If FS or OP fails, tighten eventually the belt(s).
10.13.3 F5 WASHINGS MENU
TE-OS-F5-F5-2: Fill the hydraulics if the tubes are empty, else the following tests
will be faulty.
TE-OS-F5-F5-1: Pumps test, adjustment of the reaction cuvettes level check and
! Instead of TE-OS-F5-F5-2 you
can also run the hidden com-
mand TE-OS-F5-F5-6 which re-
of the needles dispensation quote. ports more data.
TE-OS-F5-F5-5: Adjust pumps.
10.13.4 F4 READING TEST MENU
TE-OS-F4-F6-1: OP reading offset check.

TE-OS-F4-F7-2: FS filter wheel offset adjustment.
TE-OS-F4-F5-2: Autozero test. The CV% must be lower than 0.1500 for filters 1
and 2 and lower than 0.1000 for the other filters. In the column "FSOP" there
must be only zero values (it is a signal of good FS and OP positioning). If the CV%
values are anyway high with zero FSOP values, check the lamp, its connectors
and its wiring.
10.14 Diluter and pipetting test
Run the 2 μl pipetting test procedure with E124 (O.D. at 505 nm: 30 to 50 abs, 1
ml in sample tube #1).
154
10.14.1 TEST PROCEDURE FOR SAMPLING ARM 1
OK1,1
XM6
To report:
WS1
XM0
The CV% must be lower than 2.5 to 3 %. Factory value is lower than 2 %
10.14.2 TEST PROCEDURE FOR SAMPLING ARM 2
Ok1,1 (second letter lowercase)

XM6
To report:
Ws1 (second letter lowercase)
XM0
The CV% must be lower than 2.5 to 3 %. Factory value is lower than 2 %

ASTM LIS InTerfAce 155
11 ASTM LIS INTERFACE

The LIS connection is based on a text file exchange in a LAN. The text files are
encoded on the basis of the ASTM standard. Work list files are downloaded from
the LIS to the analyzer and result files are uploaded from the analyzer to the LIS.
For a detailed description, please refer to the specific document, provided by

HUMAN.
156

Error codEs 157
12 ERROR CODES
12.1 HI software error codes
Number Name Description

You are not connected to an instrument!
e0001 INSTR_CLOSE
The program will be closed now.
Unable to read firmware version from in-
e0002 UNABLE_TO_READ_
strument.
e0003 CONN_BF_PROC Connect the instrument before proceeding.
e0004 ERROR_REMOVING_ Error removing the selected method.
Method upload procedure cannot be exe-
e0005 METHOD_UPLOAD_P cuted when instrument is running or during
check of reagent levels.
e0006 PROBLEM_UPLOADI Problem uploading methods.
Error during the update tests-status func-
e0007
tion
e0008 AN_ERROR_OCCURE An error occurred while saving the settings.
e0009 SAMP_POS_NV Not valid sample position.
A linked patient should be defined to conti-
e0010 PATIENT_LINKERROR
nue with the creation of the sample.
e0011 INV_FIELD_BLANK Invalid field value for blank validity.
e0012 INV_FIELD_OD1 Invalid field value for blank O.D. 1.
e0013 INV_FIELD_STD Invalid field value for standards number.
e0014 INV_FIELD_CALVA Invalid field value for calibration validity.
e0015 INV_FIELD_FACT Invalid field value for calibration factor.
e0016 INVALID_CORRELATION Invalid value for correlation factor.
Start-up procedure has been interrupted. It
e0017 SU_FIN_INT
is recommended to repeat the procedure.
Needle rinse procedure has been inter-
e0018 NR_FIN_INT rupted. It is recommended to repeat the
procedure.
Empty tubes procedure has been inter-
e0019 ET_FIN_INT rupted. It is recommended to repeat the
procedure.
Prime hydraulics procedure has been inter-
e0020 PH_FIN_INT rupted. It is recommended to repeat the pro-
cedure.
Wash cuvettes procedure has been inter-
e0021 WC_FIN_INT rupted. It is recommended to repeat the
procedure.
Shut-down procedure has been interrupted.
e0022 SD_FIN_INT
It is recommended to repeat the procedure.
158

Only the first 40 methods have been loaded
e0023 MET_UP_PARTIAL on the instrument. Please check the number
of methods and try again.
e0024 ACCOUNT_LOG Account login error.
The maximum number of methods has been
e0025 METH_MAX_EXCEED
reached.
The current method already exists in the
e0026 METH_ALR_EXIST
method list. Operation aborted.
e0027 METH_ERR_LOAD Error loading a method.
e0028 SAMP_POS_ALR_EX This sample position is already used.
e0029 SAMP_POS_INV Invalid position for current sample.
Sample position can be changed only if the
e0030 SAMP_POS_NT_CHANG sample doesn’t have executing or scheduled
tests.
e0031 SAMP_POS_VAL_INV Invalid sample position value.
To remove an executing test, the instrument
e0032 TO_REMOVE_AN_EX
must be connected.
The instrument is not responding. Check the
e0033 THE_INSTRUMENT_ cable connection. If the problem persists,
contact the assistance.
e0034 ERR_MAIN_LOGS Error saving maintenance log
e0035 EXECUTION_STOPP Execution stopped.
e0036 NON-VOLATILE_RA Non-volatile RAM error.
e0037 LAMP_OUT-OF-WOR Lamp out-of-work.
e0038 NO_CLEAN_REACTI No clean reaction tubes.
e0039 REACTION_HIGH_T Reaction high temperature error.
e0040 ERROR_ON_SAMPLI Error on SN sampling needle home search.
e0041 ERROR_ON_WASH_S Error on AN wash station home search.
e0042 ERROR_ON_NEEDLE Error on RN needle rotation home search.
e0043 ERROR_ON_SAMPLE Error on IP Sample plate home search.
e0044 ERROR_ON_REACTI Error on OP Reaction rotor home search.
e0045 ERROR_ON_DILUTE Error on DS Diluter syringe home search.
e0046 ERROR_ON_FILTER Error on FS Filter wheel home search.
e0047 REACTION_LOW_TE Reaction low temperature error.
e0048 BARCODE_READER_ Barcode reader not ready.
e0049 WELL_WASH_DISP. Well wash dispensation pump error.
e0050 WELL_WASH_ASP._ Well wash aspiration pump error.
e0051 VACUUM_PUMP_1_E Vacuum pump 1 error.
e0052 VACUUM_PUMP_2_E Vacuum pump 2 error.
e0053 WASH_DISP._1_PU Wash dispensation 1 pump error.

Error codEs 159

Bubbles may be in the system. If problem
e0058 WASH_DISP._6_PU2 persists after prime, the cause may be the
malfunctioning of dispensation pump 6.
e0059 DOOR_OPEN:_WAIT Door open: waiting.
e0060 WASTE_FULL Waste full.
e0061 WATER_TANK_EMPT Water tank empty.
e0062 WASH_TANK_EMPTY Wash tank empty.
e0063 SAMPLE_TUBE_EMP Sample tube empty during pre-dilutions.
e0064 DILUENT_BOTTLE_ Diluent bottle empty during pre-dilutions.
e0065 NEEDLE_SHOCK_ER Needle shock error.
e0066 REAGENT_POSITIO Reagent position request out-of-range.
e0067 FILTER_POSITION Filter position request out-of-range.
e0068 FIRMWARE_ERROR Internal firmware error.
e0069 READING_A/D_OVE Optical reading A/D overflow.
Optical reading A/D time-out, A/D malfunc-
e0070 READING_A/D_TIM
tioning.
e0071 INVALID_AUTOZER Invalid autozero.
e0072 REACTION_ROTOR_ Reaction rotor dirty. Too many dirty tubes.
e0073 WASH_STATION_DO Wash station down error.
e0074 ERROR_ON_REAGEN Error on reagent plate Home search.
Error in OP reaction rotor offset calibration.
e0075 ERROR_IN_OUTER_ It is recommended to execute the optical ca-
libration.
e0076 LOW_LIQUID_LEVE Low liquid level in sample tube 1.
e0077 RINSE_BOTTLE_MI Rinse bottle missing in reagent position 1.
Needle shock detector is stuck. Shock is de-
e0078 NEEDLE_SHOCK_DE
tected in high needle position.
e0079 COMPOSED_ERROR Composed method error.
e0253 SN level sensor failed.
e0254 Power board 1 error.
e0258 Error on sampling needle 2.
e0259 Error on needle rotation 2.
e0260 Error on diluter syringe 2.
e0261 SN needle 2 shock error.
SN needle 2 shock sensor failed. Closed
e0262
when needle up.
160

e0263 SN needle 2 level sensor failed.
e0264 Well 1 wash dispension pump error.
e0265 Well 1 wash aspiration pump error.
e0617 Invalid OEM data folder.
m0008 Account log-in failed.
12.2 Firmware version 2.29D execution error codes

0 NO ERROR No error.
1 STOP_EXIT Execution stopped.
2 NVRAM_ERROR Flash memory error.
3 LAMP_OFF_ERR Lamp off.
4 NO_CLEAN_TUBES_ERR No clean reaction tubes .
5 OP_HIGH_TEMP_ERR Reaction high temperature error.
6 SN_ERR SN home error.
7 AN_ERR AN home error.
8 RN_ERR RN needle rotation home error.
9 IP_ERR IP home error.
10 OP_ERR OP home error.
11 DS_ERR DS home error.
12 FS_ERR FS home error.
13 OP_LOW_TEMP_ERR Reaction low temperature error.
14 BARCODE_ERROR Barcode reader not ready.
15 WELL1_DISP_PUMP_ERRWell wash dispensation pump error.
16 WELL1_ASP_PUMP_ERR Well wash aspiration pump error.
17 VACUUM_PUMP1_ERR Vacuum pump 1 error (switch).
18 VACUUM_PUMP2_ERR Vacuum pump 2 error (switch).
WASH_DISP_PUMP1_ Wash station pump 1 error (start-up & self-
19
ERR test).
20
ERR test).
21
ERR test).
22
ERR test).
23
ERR test) <NOT USED>.
WASH_DISP_PUMP6_ Wash station pump 5 error (start-up, self-
24
ERR test, washings).
25 WASTE2_FULL_ERROR Waste 2 (yellow) full error.

Error codEs 161

26 WASTE_FULL_ERROR Waste (red) full.
27 WATER_BOTTLE_EMPTY Water bottle empty.
28 WASH_BOTTLE_EMPTY Wash bottle empty.
29 SMP_LEVEL_ERR Only for samples dilutions off-line.
Only for dilutions, test pumps, start-up, fill
30 DIL_LEVEL_ERR
in stop.
31 SN_SHOCK_ERROR Sampling needle 1 shock error.
32 REAG_POS_ERROR Reagent position error (only diagnostics).
33 FILTER_NUMBER_ERR Filter number error.
34 FIRMWARE_ERROR Firmware error.
35 AD_OVERFLOW_ERR Optical reading A/D overflow.
36 AD_TIMEOUT_ERR Optical reading A/D time-out.
Invalid autozero (one or more filters out of
37 AUTOZERO_ERR
range).
Reaction rotor dirty. More than MXDIRT ex-
38 OP_DIRTY_ERR
cluded tubes.
39 AN_DOWN_ERR Wash station down error.
40 RP_ERR <NOT USED>
41 OP_CALIB_ERR Error in OP offset calibration (in start-up).
42 LOW_LEVEL_IP01 Low level in tube IP-01.
43 LOW_LEVEL_RINSE Low level rinse bottle in R01/R02.
SN needle shock sensor failed (closed when
44 SN_SHOCK_FAIL
needle up).
45 SN_LEVEL_FAIL SN needle level sensor failed.
46 PWR_BRD0_ERR Power board 0 error.
50 SN2_ERR Error on sampling needle 2.
51 RN2_ERR Error on needle rotation 2.
52 DS2_ERR Error on diluter syringe 2.
53 SN2_SHOCK_ERROR SN needle 2 shock error.
SN needle 2 shock sensor failed (closed
54 SN2_SHOCK_FAIL
when needle up).
55 SN2_LEVEL_FAIL SN needle 2 level sensor failed.
56 WELL2_DISP_PUMP_ERRWell 2 wash dispensation pump error.
57 WELL2_ASP_PUMP_ERR Well 2 wash aspiration pump error.
58 SN_MISSING_ERR Instrument S/N missing.
Diluent bottle empty on tray 2 (used in dia-
59 DIL2_LEVEL_ERR
gnostics).
Pipettor 1 is not aspirating and dispensing
60 PIPETTING1_ERR
properly. Pinch valve problem.
162

Pipettor 2 is not aspirating and dispensing
61 PIPETTING2_ERR
properly. Pinch valve problem.
62 METHODS_ERROR At least one method is in error.

SyStem PARAmeteRS LISt 163
13 SYSTEM PARAMETERS LIST
13.1 IP motor
Number Name Default Description

000 IPNPS0 30 Number of positions on the outer ring
001 IPNPS1 30 Number of positions on the inner ring
002 IP0SN1 300 Sampling needle offset for the outer ring
003 IP1SN1 369 Sampling needle offset for the inner ring
004 Q004
Reverse rotation for short movements
005 IPMODE 0 0 = disabled
1 = enabled
006 IPPHOM 27 Pre-position for fast home
007 IPOPER 350 Delta steps for tube in front of operator
Type of sample tray
0 = free, PTU
008 IPTYPE 2
1 = 20 PTU + 20 CUP2
2 = 30 PTU + 30 PTU
Homing after every sample
009 IPHOME 0 0 = disabled
1 = enabled
010 IPOFBC 1550 Samples barcode offset
Numbering shift between inner and outer ring
011 IPSHFT 0
0 = inner follows with half step (for barcode use)
Swap type
012 IPSWAP 0
1 = alternate type
013 IPDOF0 7 Outer ring delta offset from 1st to 2nd sample tray
014 IPDOF1 61 Inner ring delta offset from 1st to 2nd sample tray
015 Q015
... ...
018 Q018
13.2 OP motor

019 OP_SN1 691 Offset SN1 side
020 OPOFWS 471 Offset WS side
021 OPWSRD 10 Offset between WS and RD (in tube positions)
022 OPOFRD 275 Offset reading side
023 OPPHOM 73 Pre-positionning for home speed-up
164

1 = move away if close to home position
024 OPHOME 1
2 = no home during run
Offset between WS and tube change (in tube
025 OPCHTU 40
positions)
Offset between WS and SN1 (in reaction cell
026 OPWSN1 70
position)
Number of R1/R2 incubation cycles = offset
027 OPR1R2 5
between R1 and R2
Number of R2/R3 incubation cycles = offset
028 OPR2R3 2
between R2 and R3
029 Q029
030 OPOFGO 32 Tube for reading gain + offset
13.3 FS motor

031 FSOFS1 -160 FS1 wheel offset
035 FSOFS5 0 FS5 wheel offset
13.4 SN motor

040 Q040
041 SN1HOM 14 SN1 high out of FC for fast home search
042 SN1LOW 744 SN1 low position over the reaction tubes
043 SN1MID 70 SN1 mid position over the sample tubes
044 SN1HIG -30 SN1 high, for rotation over reagent bottles
045 SN1WSH 764 SN1 for wash needle 1 in wash well
046 SN1WEL 336 SN1 low position over the wash well
047 SNDWEL 100 Delta to SN1WEL for dispensation in wash well
048 SN1DWU 140 SN1 delta SN1 up for drop


049 SN1REL 1400 SN1 at the bottom of large reagent bottles
050 SN1CUP 878 SN1 for sampling on IP = cups
051 SN1PTU 1400 SN1 for sampling on IP = primary tubes
SN1 upside delta/SN1DS2 for mixing in OP
052 SN1DDS 140
cuvette
SN1 for 2nd dispensation in OP, with mixing,
053 SN1DS2 1250
corresponding to ± 50 µl in OP
054 SN1CU2 1074 SN1 for inner row cups for IP 20+20
055 SN1RET 1104 SN1 at the bottom of reagent bottle type = tube
056 SN1REC 400 SN1 at the bottom of reagent bottle type = cup
057 SN1DLC 1350 SN1 at the bottom of diluent bottle
Delta to SN1WSH for level sensing in needle
058 SNDLWE 80
wash well test
Delta to SN1WSH-SN1DWU for external needle
059 SNDXWN 40
wash
SN1 at the bottom of small reagent bottles
060 SN1RES 1330
Default = SN1REL - 20 (20 units = 3 mm)
SN mixing mode
0 = bottom
061 SNDMOD 1
1 = bottom-up
2 = up-bottom
062 MIXMOD 0 Mixing type
063 Q063
064 Q064
065 Q065
13.5 DS motor

066 DS1KAD 0 DS1 steps correction
067 MIXVO1 70 DS reaction mixing volume 1st dispensation
DS type
068 DSTYPE 3
3 = EDIF 368 µl
069 DSOFFS 0 DS home offset
070 DSTROK 368 DS volume (full stroke)
071 DSTEPS 5200 DS steps/stroke
072 MIXVO2 100 DS reaction mixing volume 2nd dispensation
073 MIXRP1 1 DS reaction mixing repetitions dispensation 1
074 MIXRP2 2 DS reaction mixing repetitions dispensation 2
166

075 DLYMIX 5 Delay in needle mixing in tenths of seconds
076 DSGAP1 10 DS first gap
077 DSGAP3 2 DS third gap
078 DSGAPH -2 DS gap after home
079 MIXRPD 3 DS predilution in OP mixing repetitions
13.6 RN motor

080 NREAG 19 Number of reagent positions
081 Q081
082 RN1RE1 -98 Position of 1st reagent bottle
083 RN1REN 554 Position of last reagent bottle
084 Q084
085 RN1WRP -576 Wrap position
086 RN1WSH -8 Position on wash well
087 RN1DWN 9 Delta for wash on well SN1 side
088 RNDWEL -20 Delta for check wash needles pumps
089 RN1FSH -4 Delta for needle flush
090 RN1IP0 112 Needle 1 on IP outer ring
091 RN1IP1 149 Needle 1 on IP inner ring
092 RN1_OP 39 Needle 1 on OP tubes
Delta offset on IP outer ring from 1st to 2nd
093 RNDIP0 9
sample tray
Delta offset on IP inner ring from 1st to 2nd
094 RNDIP1 28
sample tray
095 RN1DLC 504 Position on clinical chemistry diluent bottle
096 RN1PHM 50 Pre-home position
097 RN1MOD 0 Home mode
098 Q098
099 Q099
100 MLCURR 64 Large motor current
101 MSCURR 45 Small motor current
102 Q102
... ...
109 Q109

13.7 WS motor

110 Q110
111 WSHOME 0 Home position
High position out of the optical switch under
112 WSHIGH 16
home
1st pause during wash aspiration / rising start
113 WS_A1D -128
vacuum aspiration
114 WS_A2D -60 2nd pause during wash aspiration
115 WS_ASP 428 Bottom wash aspiration
116 WSZMOD 0 Home search mode
117 WSTUBE -360 Low immediately over the OP reaction tubes
118 Q118
119 Q119
13.8 Motor speeds, home tolerances

120 IPVEL 450 IP normal speed
121 IPVELZ 313 IP home speed
122 IPDYBC 6 IP delay in barcode scanning
123 Q123
124 Q124
125 OPVEL 900 OP normal speed
126 OPVELZ 250 OP home speed
127 OPVELR 900 OP reading speed
128 OPVELS 1600 OP reading scan speed
129 FSZMOD 1 FS home mode
130 FSVEL 1800 FS speed
131 FSVELZ 550 FS home speed
132 DLYFLT 5 Delay for filter rotation in reading
133 DSVELD 1800 DS dispensation speed
134 DSVELA 1600 DS aspiration speed
135 DSVLOW 1400 DS speed low (gap + sample aspiration)
136 Q136
137 DSVELZ 1200 DS home speed
138 RNVEL 1500 RN speed
139 RNVELZ 500 RN home speed
140 Q140
... ...
168

143 Q143
144 SNVELU 1600 SN up speed
145 SNVELD 1600 SN down speed
146 SNVELZ 700 SN home speed
147 SNVELL 1600 SN low speed for level detection
148 Q148
149 WSVELH 1200 WS high speed
150 WSVELL 250 WS low speed
151 WSVELZ 400 WS home speed
152 Q152
153 IPZERR 2 Steps limit for IP home step error
154 RNZERR 2 Steps limit for RN home step error
155 OPZERR 1 Steps limit for OP home step error
156 SNZERR 4 Steps limit for SN home step error
157 FSZERR 1 Steps limit for FS home step error
158 WSZERR 1 Steps limit for WS home step error
159 DSZERR 8 Steps limit for DS home step error
13.9 Pumps

160 DIWSH1 38 Water dispensation 1 time (tenths of second)
Needle 1 reaction aspiration time (tenths of
165 ASPWH1 100
second)
166 ASVAC4 300 Fourth vacuum aspiration (tenths of second)
167 ASVAC1 170 First vacuum aspiration (tenths of second)
168 ASVAC2 200 Second vacuum aspiration (tenths of second)
169 ASVAC3 100 Third vacuum aspiration (tenths of second)
170 DLYPMP 10 Delay after pump stop, before ASVAC3
2 lower digits = rep. for wash (wash prime on 2nd
171 FILLWS 4090 needle)
2 higher digits = rep. for water prime
Number of repetitions of fill/aspiration cycles in
172 NRIPWS 1
the WS
173 ENWSDN 1 Enable check WS down limit switch


Enable pump 6 wash dispension error in WS
174 EPM5ER 2
cycle
Enable pumps check in fill hydraulics (summed)
1 = dispensation well
2 = aspiration well
175 FILCHK 31
4 = test well
8 = WS aspiration
16 = pipetting/valve
Enable pumps wash dispensation error in test
176 EPMDER 1
WS dispensation
177 WSHVOL 400 Target volume (µl) for WS
178 Q178
179 MINAS1 Minimum aspiration 1 volume in pumps test
THOMAS peristaltic pump dispensation µl per
second conversation factor,
180 THMCNV 800
to calculate water and waste variations
(measured range = 700 to 1,050)
Wash needle in well mode
181 NDWMOD 1 0 = center
1 = side + center
Wash dispensation,
182 NDWSHO 65 SN in OP tube (tenths of second for 400 µl)
SN_Wash()
Wash dispensation, SN in well/samples 1st + 2nd
183 NDWSHS 120
dispensation (tenths of second)
Wash dispensation, SN in well/reagents check
184 NDWSHR 120
level, R3, R4 (tenths of second)
Wash dispensation, SN in well/external wash
185 NDWSHX 50
(tenths of second)
Well aspiration extension after well
186 ASWELD 100
dispensation
Well dispensation delay after pump stop,
187 DIWELD 40
to avoid drop
Well external wash delay after pump stop,
188 EXWELD 10
to avoid drop
-1 = WASH_NEEDLE_PRE
189 WNMODE 1 1 = WASH_NEEDLE_POST
2 = WASH_NEEDLE_POST, wait END-OF-WN
190 Q190
191 ASVAV1 100 Vacuum pump phase 1 speed (50 - 100)
170

Needle 2 dispensation pump speed,
194 EXWL2V 60
only for external wash (%)
Needle 1 dispensation pump speed,
195 EXWL1V 60
only for external wash (%)
Needle dispensation pump speed,
196 INWELV 100
only for internal wash (%)
197 PERILF 500 Peristaltic pump expected life (hours)
198 VAC1LF 500 Vacuum pump 1 expected life (hours)
199 VAC2LF 500 Vacuum pump 2 expected life (hours)
13.10 Various parameters

Current software release for automatic
200 RELEAS 229
parameter update
201 Q201
202 ENASIC 1 Enable door security limit switch
203 PARAM1 0 Reserved for diagnostics
204 ENALOG 1 Enable LOG prints
Automatic save screen in a folder named
"HiTerminal_AutoScreen\AutoScreen_YYYY_
205 SVSCRN 0 MM_DD_HH_MM" on the desktop
0 = disabled
1 = enabled
Reserved
206 SPAUSE 0 -1 = Sstep
>1 = breakpoint at SStatus = SPAUSE
207 DISPLV 0 Reserved for diagnostics
208 Q208
Enable vacuum error
209 ENVACS 3 1 = needles aspiration test with SN level
2 = swab aspiration test with SN level
210 Q210
211 EEXCHK 1 Enable execution flag check
212 ESWR12 1 Enable reagents swap in R1 + R2 dispensation
213 MKRUNL 0 Reserved for internal use
Read mode
0, 1 = data flow
214 RDMODE 2
2 = program flow
3 = fast scan mode
215 RDSTEP 3 Optical reading rate (every 1 or 2 cycles)


216 NDISP 1 Number of dispensations per cycle
Beginning of 3rd dispensation (sec)
217 PHASD3 0
0 = no 3rd dispensation
218 MCYCLE 24 Machine cycle (sec)
Beginning of 2nd dispensation (sec)
219 PHASD2 10
0 = no 2nd dispensation
220 EWNREA 1 Enable needle external wash after REAG
Enable needle external wash after SMP if
221 EWNSMP 3
volume ≤ EWNSMP
Enable WS in run
222 ENWSHS 1
0 = for diagnostics only
Number of wash prime at Run start
223 NWPRIM 5
0 = disabled
224 NSPWSH 1 Number of cuvettes special washes (1 or 2)
225 Q225
Lamp supply DAC hardware
0 = old power board
Lamp voltage 5.1 to 6.0 V. Stand-by: 5.1 V.
226 LMPDAC 1 Operation: 5.9 V
1 = newer board
Lamp voltage 3.3 to 6.0 V. Stand-by: 3.3 V.
Operation: 5.9 V
227 LMPGAI 5 Lamp voltage setting (0 - 20)
Lamp saving delay after switch on (sec)
0 = deactivated
228 LMPSAV 200
X = X seconds delay after switch on (200 is
recommended)
229 LMPLIF 1000 Optical lamp expected life (hours)
13.11 Barcode

Internal barcode scanner
230 BARCOD 1 0 = not installed
1 = installed
231 Q231
232 BOARD0 1 Power board 0 installed
172

Enable fatal error on power board
235 ENBDER 0 communication error
(PWR_BRDx_ERR; x = 0 to 3; e0046 to e0049)
Enable log messages
236 LOGMSG 0
1 = enabled
Enable system error METHODS_ERROR 62
237 METHER 0
0 = disabled
238 Q238
239 Q239
13.12 Level sensor, floats

Primary tube sample level check
240 LEVPTU 14
-1 = no, else steps before needle stop
Cups sample level check
241 LEVCUP 10
Reagent level check
242 LEVREA 14
Sample diluent level check
243 LEVDIL 14
Sample diluent in OP level check
244 LEVCUV 8
Primary tube death volume calibration in tenths
245 KLVPTU 57
of µl per SN step
Sample cup death volume calibration in tenths of
246 KLVCUP 30
µl per SN step
Large reagent bottle death volume calibration in
247 KLVREL 595
tenths of µl per SN step
Small reagent bottle death volume calibration in
248 KLVRES 230
tenths of µl per SN step
Diluent bottle death volume calibration in tenths
249 KLVDLC 595
of µl per SN step
Primary tube sample maximum level (steps)
250 LMXPTU 1260
above which detection is disabled
Cup sample maximum level (steps) above which
251 LMXCUP 400
detection is disabled
Reagent maximum level (steps) above which
252 LMXREA 1360
Diluent maximum level (steps) above which
253 LMXDLC 1360
254 Q254


Reagent large bottle maximum level (ml) to
255 RELVOL 70
which measured level is clipped
Reagent small bottle maximum level (ml) to
256 RESVOL 25
which measured level is clipped
Reagent tube maximum level (ml) to which
257 RETVOL 5
measured level is clipped
Reagent cup maximum level (ml) to which
258 RECVOL 1
measured level is clipped
259 Q259
Full volume of waste (ml)
260 WSTFUL 19000
= fatal alarm
Reserve volume of waste full (ml)
261 WSTRES 17000
= float commutation
Full volume of water (ml)
262 WATFUL 19000
= fatal alarm
Reserve volume of water (ml)
263 WATRES 1000
= float commutation
264 WATRS2 400 Reserve alarm volume of water (ml)
265 WSHFUL 2000 Full volume of wash (ml)
Reserve volume of wash (ml)
266 WSHRES 200
= float commutation
267 WSHRS2 100 Reserve alarm volume of wash (ml)
Full volume of waste 2 (ml)
268 WST2FL 1900
= fatal alarm
Reserve volume of waste 2 full (ml)
269 WST2RS 1700
= float commutation
1 = delete reagents at power-on
270 DELREA -1 0 = don't delete
-1 = keep also levels
271 Q271
Maximum difference error in sample level double
272 DSMPLV 50
check (µl)
273 EFLOAT 15 Enable waste and wash floats alarm
274 ESHOCK 3 Enable shock sensor
275 Q275
276 Q276
277 REF150 42 OP target level in steps for 150 µl
278 OPL150 42 OP measured level in steps for 150 µl
279 OPL450 175 OP measured level in steps for 450 µl
174
13.13 Temperatures

280 OP1TMP 380 Normal OP temperature (tenths of °C)
281 MAXRS1 70 Maximum duty-cycle (%) for RS1 OP heating
Fixed reagent pre-heating maximum duty-cycle
282 PR1PWM 0
(%)
283 OPTMPO 0 OP temperature offset (tenths of °C)
284 OPTSBY 0 Standby OP temperature (tenths of °C)
285 OPTCAL 0 Temperature calibration (half tenths of °C)
286 Q286
287 Q287
288 TMPERR 5 Temperature error for alarm (tenths of °C)
289 TMPTOU 2400 Temperature timeout for alarm (sec)
290 Q290
... ...
299 Q299
13.14 Dilutions, pipetting

300 DLYASR 50 Delay aspiration reagent (clinical chemistry)
301 DLYASS 60 Delay aspiration sample (clinical chemistry)
Delay dispensation diluent/reagent/sample
302 DLYDSP 40
(clinical chemistry)
303 Q303
304 VOLDIL 200 Pre-dilutions base (dead) volume (µl)
305 DILSHK 30 Pre-dilutions shake time (sec)
306 MIXDIL 2 Pre-dilution needle mixing repetitions
307 VOLMIN 210 Minimum reaction volume
308 MXDIL2 10 Maximum in-needle dilution rate
309 MINSMP 2 Minimum sample volume
Maximum sample number
Higher positions for wash solutions/diluents
60 = no wash solutions/diluents (60 positions
310 NSAMPL 60
sample tray)
40 = no wash solutions/diluents (40 positions
sample tray)
311 PNJVOL 40 Post-injection wash volume (µl)
312 ECWVOL 330 Extra cuvette wash volume
313 ENWVOL 150 Extra needle wash volume

13.15 Readings

Reserved for fast scan mode
314 SCNMOD 0 0 = peak
1 = average
Self Blank mode
315 SLFMOD 1 1 = check bubble
2 = don't substract tube in compensation
316 NRSLFB 3 Number of readings employed in the self-blank
317 Q317
Initial sampling tube in run (for diagnostics)
318 INISTU 0
0 = auto
Auto-OP gain
319 AUTOPG 0
1 = enabled
Filter 1 wavelength (nm)
320 FILTR1 340
Only the default value is allowed
321 FILTR2 405 Filter 2 wavelength (nm)
329 FILAUX 0 Auxiliary filter for colorimetric tests
330 ZIF0 0 F0 factory initial autozero value
331 0 Current filter 1 autozero value
340 FITMIN 980 Minimum fit for kinetics (thousandths)
Current optical gain
341 OPGAIN 75
-1 = disabled
342 OPRDER 1 Enable CancelLastReading() in OP home ≠ 0
Calculation mode (internal use)
343 CLCMOD 0
0 = no calculations
176

Enable linearity processing
0 = No
344 ENALIN 1
1 = algebraic value
2 = absolute value
345 ELSNGP 1 Enable singular points elimination
&1 = Enable substract tube zero in KINE/FIXT
&2 = Enable substract tube zero in BICR+COLOR
346 ENTUBZ 3
&4 = Substract current tube value for reference
filter
Enable kinetic adjustment of the number of
347 ADJKIN 0
readings (minimum fit to pass the adjustment)
348 ADWDTH 0 Reserved for fast scan (must be even)
349 ADFILT 2000 Reading A/D filter
Maximum autozero difference in thousandths /
350 DIFAUZ 25
previous autozero (50 = 21 mAbs)
Maximum autozero difference in thousandths in
351 DIFAZ2 15 nd
2 repetition (12 = 5 mAbs)
352 MINAUZ 5000 Minimum A/D value for autozero
Maximum zero value at filter 1 (UV) for a
353 MAXZER 1200
reaction cuvette (tenths of mAbs)
Maximum zero absolute difference between
354 MAXDZE 1000 start-up FILT_CHK and current FILT_CHK (tenths
of mAbs)
Maximum zero value at visible filters for a
355 MAXZE2 800
reaction cuvette (tenths of mAbs)
356 RPTUBZ 4 Fast tube zero repetitions
Maximum number of excluded tubes for error
357 MXDIRT 26
cuvette rotor dirty
358 SHRTRD 0 Enable short reading
359 SHRTMD 2 Short read mode
13.16 2nd arm SN2 motor

360 SN2HOM 14 High out of FC for fast home search
361 SN2LOW 744 Low position over the reaction tubes
362 SN2MID 70 Mid position over the sample tubes
363 SN2HIG -30 High for rotation over reagent bottles
364 SN2WSH 754 For wash needle 1 in wash well
365 SN2WEL 336 Low position over the wash well
366 SN2REL 1350 At the bottom of large reagent bottles


367 SN2CUP 878 For sampling on IP cups
368 SN2PTU 1400 For sampling on IP primary tubes
For 2nd dispensation in OP, with mixing,
369 SN2DS2 1248
corresponding to ± 50 µl in OP
370 SN2CU2 1074 For inner row cups for IP 20 + 20
371 SN2RET 1104 At the bottom of reagent tube
372 SN2REC 400 At the bottom of reagent cup
373 SN2DLC 1350 At the bottom of diluent bottle
At the bottom of small reagent bottles
374 SN2RES 1330
Default = SN2REL - 20 (20 units = 3 mm)
375 Q375
376 Q376
377 Q377
378 OPL152 42 OP level 2 calibration 150 µl
379 OPL452 175 OP level 2 calibration 450 µl
13.17 2nd arm RN2 motor

380 RN2RE1 -100 Position of 1st reagent bottle
381 RN2REN 548 Position of last reagent bottle
382 RN2WRP -576 Wrap position
383 RN2WSH -15 On wash well
384 RN2IP0 106 Needle 1 on IP outer ring
385 RN2IP1 144 Needle 1 on IP inner ring
386 RN2_OP 33 Needle 1 on outer plate tubes
387 RN2DLC 501 On clinical chemistry diluent bottle
388 RN2ISE 443 On ISE module
389 RN2FSH -4 RN delta for needle flush
390 IP0SN2 865 IP/SN2 needle offset for outer ring
391 IP1SN2 931 IP/SN2 needle offset for inner ring
392 Q392
393 Q393
394 OP_SN2 1266 OP offset SN2 side
Cuvette position under sampling needle 2, when
395 OPWSN2 41
cuvette 1 is under wash station 1 position
396 DS2KAD 0 Diluter syringe 2 steps correction
397 Q397
398 Q398
399 Q399
178
13.18 Miscellaneous

400 SN_ACC 100 SN acceleration
401 SN_DEC 150 SN deceleration
402 FS_ACC 120 FS acceleration
403 RN_ACC 400 RN acceleration
404 OP_ACC 400 OP acceleration
405 IP_ACC 600 IP acceleration
406 Q406
... ...
418 Q418
419 BOOTER 3 Reserved for internal use
420 OPFMOD 0 OP offset find mode
421 Q421
... ...
437 Q437
438 CPU_HW 251 CPU board version
439 CODCHK XXXX Weighted parameters checksum

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 • 65205 Wiesbaden • Germany
Tel.: +49 6122/9988 0 • Fax: +49 6122/9988 100
eMail: human@human.de • www.human.de

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HS300SR Service Manual R1 PDF

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HumaStar 300SR

Cat. No. 169302 - Revision 1

REVISION/DATE REVISION DESCRIPTION

SERVICE UND SUPPORT

4.7.2.3 Sampling needle wash well (ARM 1 and ARM 2) 58

6.7 VACUUM PUMP REPLACEMENT 107

7 FIRMWARE UPDATING 123

8 HI SOFTWARE INSTALLATION 129

10.8 DILUTER 151

11 ASTM LIS INTERFACE 155

12 ERROR CODES 157

13 SYSTEM PARAMETERS LIST 163

1.2 User Warranty

HUMAN warrants that instruments sold by one of its authorised

1.3 Intended Use of the Instrument

1.4 General Safety Warnings

Use only chemical reagents and accessories specified and supplied by

1.5 Disposal Management Concept

The applicable local regulations governing disposal must be observed. It

HumaStar 300SR | Service manual

1.6 Biohazard Warning

Analytical instruments for in vitro diagnostic application involve the handling

1.7 Instrument Disinfection

Before performing any servicing on the instrument it is very important to

HumaStar 300SR | Service manual

Clinical chemistry and turbidimetry

Removable sample tray

2 removable reagent trays

8-step cuvette wash station

External computer required

Optional ISE module

220-240 or 110-120 V, 50/60 Hz

HumaStar 300SR | Service manual

3.1 General description

HumaStar 300SR performs both clinical chemistry and turbidimetric immuno-

3.2 Main characteristics

3.3 Installation and removal from service

3.3.1 Shipping and delivery

The analyzer is wrapped in stretch-plastic and securely fixed within a strong

The shipment of the analyzer generally is the responsibility of the distributor

If the shipment arrives by private or commercial carrier, immediately inspect the

3.3.4 Before setting-up

Prepare a location for your analyzer based on maximum laboratory efficiency

HumaStar 300SR | Service manual

! The top cover should be lifted

However, the cardboard carton of miscellaneous items and accessories may be

HumaStar 300SR | Service manual

3.3.5.1 Left side

3.3.5.2 Right side

Connect the mouse to a PC port.

! Adaptors may be required for

Typically instruments are delivered with "Schuko" connections.

3.3.5.4 Instrument connections

Connect a USB cord to a PC port and to the analyzer port.

3.3.5.5 Hydraulic connections

Place the 4 tanks below the working level.

HumaStar 300SR | Service manual

! Hydraulic tubes have easy-to-

! Ensure that the metal tab of

! The presence of bubbles in

3.4 Main components

HumaStar 300SR | Service manual

3.4.1 CPU board