Professional Documents
Culture Documents
Gel Tubes
• What is the purpose of the gel in BD Vacutainer® SST™ Serum Separation Tubes
and BD Vacutainer® PST™ Plasma Separator Tubes?
The gel forms a physical barrier between serum or plasma and blood cells during
centrifugation. It is important to note that after collection, BD Vacutainer® SST™ Serum
Separation Tubes should be inverted five times, allowed 30 minutes clotting time, and
centrifuged for 10 minutes at 1000-1300 RCF (g) in a swing bucket centrifuge. BD
Vacutainer® PST™ Plasma Separation Tubes should be inverted 8 times, and
centrifuged for 10 minutes at 1000-1300 RCF (g) in a swing bucket centrifuge.
• What is the difference between BD Vacutainer® SST™ and PST™ Blood Collection
Tubes?
SST™ refers to the Serum Separator Tube containing clot activator and serum separator
gel. PST™ refers to the Plasma Separator Tube containing lithium heparin and plasma
separator gel.
• What is the gel composed of?
The gel is composed of inert components, which are part of a polyester-based proprietary
formulation.
• What is the clot activator in BD Vacutainer® SST™ Serum Separation Tubes?
The silica particles that coat the walls of the BD Vacutainer® SST™ tube are the clot
activator. Initial activation occurs when blood enters the tube and contacts the particles
on the tube wall. To continue the activation process, it is necessary to thoroughly mix the
blood and particles by inverting the tube five times.
• Why do the inside walls of the BD Vacutainer® SST™ Tubes appear white and
cloudy? Are the tubes still all right to use?
The walls of BD Vacutainer® SST™ Serum Separation Tubes are coated with silica
particles as a clot activator. The coating process creates a film on the tube surface that
appears white and slightly cloudy. The tubes are fine to use. However, it is important to
remember to invert the SST™ Tube at least five times after filling. This ensures adequate
mixing of silica particles with the blood, which is required for optimal performance.
• What is an SST™ transport tube?
The BD Vacutainer® SST™ Serum Separation Transport Tube contains double the
amount of gel compared to the regular SST™ tubes. This provides a thick barrier that
remains intact when tubes are transported, thereby maintaining the quality of the sample
for the lab analysis. It is intended for use primarily when specimens are collected and
centrifuged in physician laboratories or other remote collection stations, and then
transported back to the laboratory for analysis.
• How soon after collection should BD Vacutainer® gel tubes be centrifuged?
Gel separation tubes should be centrifuged no longer than 2 hours after collection.
• Can I re-centrifuge BD Vacutainer® gel tubes?
BD does not recommend re-centrifuging gel tubes once the barrier has formed.
• Can the serum and plasma be frozen on the gel, in the original BD SST™ and
PST™ Tubes?
It is not recommended to freeze the sample in the primary blood collection tube, on the
gel barrier. The gel may separate when it is frozen and thawed, resulting in red cell
contamination of the sample.
• Can the BD SST™ Tubes be used for therapeutic drug monitoring (TDM)?
It is not recommended to use the BD gel tubes for any tri-cyclic antidepressant drug
testing. BD has done studies using the BD SST™ tubes for 16 other therapeutic drugs.
The white paper may be accessed on the BD website at www.bd.com/vacutainer under
the section entitled “Library of Clinical Documentation.”
• Can the BD SST™ tubes be used for trace element testing?
BD has not validated the SST™ tubes for trace element testing. It is recommended to use
the royal blue stoppered trace element tubes for trace element testing.
EDTA Tubes
• What does EDTA stand for and how does the anticoagulant work?
EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium
in the blood and keeping the blood from clotting.
• What is the concentration of EDTA in the lavender and pink stoppered tubes?
The nominal EDTA concentration is 1.8mg EDTA per milliliter of blood.
• Why do some BD Vacutainer® lavender top blood collection tubes contain K2EDTA
and others contain K3EDTA?
BD Vacutainer® Plus Blood Collection Tubes contain K2EDTA, which is spray-dried to
the walls of the tube. K2EDTA is recommended by the CLSI (CLSI guideline -
Evacuated Tubes and Additives for Blood Specimen Collection-Fifth Edition H1-A5,
2003) and the ICSH (International Council for Standardization in Hematology). BD
Vacutainer® glass blood collection tubes contain K3EDTA, the liquid form of EDTA.
• What differences can I expect to see in hematological parameters when I switch
from BD Vacutainer® K3EDTA to BD Vacutainer® K2EDTA?
K3EDTA is a liquid and will dilute the sample ~ 1-2%. K2EDTA is spray-dried on the
walls of the tube and will not dilute the sample. Slightly higher results have been seen
from the K2EDTA tube in some CBC parameters, but none of these were clinically
significant. It is important to mix the sample 8 - 10 times immediately after collection to
ensure all the anticoagulant is thoroughly mixed with the blood sample.
• Can the BD Vacutainer® Plus Plastic EDTA Tube be used for routine blood bank
procedures?
Yes, the BD Vacutainer® K2EDTA Plus Tubes are intended for routine
immunohematology testing, including red cell grouping, Rh typing and antibody
screening.
• What are the differences between the lavender stopper BD Vacutainer® K2EDTA
Tube and the pink stopper BD Vacutainer® K2EDTA Tube?
The differences are the types of closures and the labeling. The lavender stopper can either
be rubber or a Hemogard™ closure on a plastic tube. Product 367899, a 6 mL plastic
tube, and product 367842, a 2 mL plastic tube, both have a distinct pink BD Hemogard™
closure and product 368589 has a conventional rubber pink stopper. The pink stoppered
tubes have blood bank labels and are generally sent to the blood bank laboratory in the
hospital. The additive is the same in both tubes.
• What is the minimum and maximum fill volume in the EDTA tubes?
The minimum and maximum acceptable blood volumes should be established by each
facility, to ensure that accurate hematology results are obtained. BD Vacutainer® Blood
Collection Tubes are designed to draw the appropriate volume to ensure a proper blood to
additive ratio.
• Does BD offer an EDTA tube that contains gel?
Yes, the BD Vacutainer® PPT™ Tube (reference number 362788) contains EDTA
anticoagulant and an inert polyester gel. The Hemogard closure color is pearl white and
the blood draw volume is 5mL.
Heparin Tubes
• What is the source of the heparin that is used in the BD Vacutainer® Blood
Collection Tubes?
The heparin is derived from porcine intestinal mucosa.
• What types of heparin are offered in the BD Vacutainer® Blood Collection Tubes?
The green top heparin tubes contain either the lithium or sodium salt of the heparin
anticoagulant. Lithium heparin should not be used for lithium determinations.
• How is the heparin applied to the tube wall?
All of the heparin is spray-dried on the wall of the tube.
• What is the concentration of heparin in the BD Vacutainer® Blood Collection
Tubes?
The heparin concentration is approximately 15 USP (US Pharmacopeia) units of heparin
per milliliter of blood. The USP is not equivalent to the International Units (IU). The IU
is 6.4% greater than the USP.
• Will the sodium results be higher in the Sodium Heparin Tubes?
When the Sodium Heparin tubes are filled to the proper volume, the sodium results will
only be 1 to 2 mmol/mL higher.
Trace Element/Lead Tubes
SPS Tubes
ACD Tubes
• What are the uses of the BD Vacutainer® Safety-Lok™ Blood Collection Set
(SLBCS)?
The BD Vacutainer® Safety-Lok™ Blood Collection Set is simple, easy to use, and is
safety-engineered. The safety mechanism can be activated immediately after the blood
draw and helps protect against needlestick injury. It is also offered with a pre-attached
holder for added convenience and to help ensure OSHA single-use holder compliance.
• Can BD Vacutainer® Blood Collection Sets, BD Safety-Lok™ Blood Collection Sets
and BD Vacutainer® Push Button Blood Collection Set be used for intravenous
administration of fluids or blood collection procedures?
Yes, all of these products can be used to administer intravenous fluids for short-term (up
to 2 hours) and for blood collection.
• I had trouble activating the safety shield of the BD Safety-Lok™ Blood Collection
Set. What went wrong?
Withdraw the BD Safety-Lok™ blood collection set by grasping the translucent yellow
safety shield grip area with the thumb and index finger. Note: If you grasp the shield
along the sides, you will prevent the wings from sliding back along the track inside the
shield. The thumb should be on top of the grip area. With the opposite hand, grasp tubing
between thumb and index finger. Push the yellow shield forward until the safety shield is
locked in place and you hear a click. Discard into an approved sharps container.
• Why do I need to hold the wings during insertion? Why can't I just hold the sides of
the safety shield?
Inserting the needle by holding the wings will give you a greater sense of control to
perform the phlebotomy. If you hold the sides, the safety shield will slide forward over
the needle when the point of the needle makes contact with the skin. Continue to use your
current technique of insertion by holding the wings. Remember, after insertion, the wings
are never touched again.
• Why can't I withdraw the BD Safety-Lok™ Blood Collection Set from the patient
by grasping a wing?
The wings are attached to the needle and then to the tubing. Holding the wings will not
allow the safety shield to be pushed forward and locked into place.
• Is the BD Vacutainer® Safety-Lok™ Blood Collection Set (SLBCS) available with a
pre-attached holder?
Yes, the SLBCS is available with a pre-attached holder.
• Where can I order the BD Vacutainer® Push Button Blood Collection Set and the
BD Safety-Lok™ Blood Collection Sets?
From an authorized BD distributor.
• Does the BD Vacutainer® Safety-Lok™ Blood Collection Set (SLBCS) contain
latex?
No, the BD Vacutainer® Safety-Lok™ Blood Collection Set is latex free.
• What needle gauges are available for the BD Vacutainer® Safety-Lok™ Blood
Collection Set (SLBCS) and the BD Vacutainer® Push Button Blood Collection Set
(PBBCS) ?
The PBBCS and SLBCS are available in 21, 23 and 25 gauge needles.
• How are BD Vacutainer® Push Button Blood Collection Set and the BD
Vacutainer® Safety-Lok™ Blood Collection Sets packaged?
Both the BD Vacutainer® Push Button Blood Collection Sets and BD Safety-Lok™
Safety Winged Blood Collection Sets are packaged 50/box and 200/case.
• Can the BD Vacutainer® Push Button Blood Collection Set (PBBCS) and the BD
Vacutainer® Safety-Lok™ Blood Collection Set (SLBCS) be used with a syringe?
The BD Vacutainer® Push Button Blood Collection Set and the BD Vacutainer® Safety-
Lok™ Blood Collection Set can be used with a syringe when it is used without a luer
adapter. The syringe will attach to the female end of the BD Vacutainer® Push Button
Blood Collection Set or the Safety-Lok™ Blood Collection Set. Once the blood has been
drawn into the syringe, ensure that the safety mechanism on the winged needle set is
properly activated. The use of any needle for the purpose of transferring blood directly
from a syringe to a specimen container continues to be prohibited by Federal OSHA. The
syringe should be attached to a device, such as the BD™ Blood Transfer Device, that
meets all procedural and safety standards for blood transfer.
• When and why must the air be purged from the BD Vacutainer® Safety-Lok™
Blood Collection Set (SLBCS) and the BD Vacutainer® Push Button Blood
Collection Set (PBBCS) tubing?
It is critical to purge the air from the tubing of a blood collection set when collecting a
sodium citrate tube for PT and/or APTT testing. A plain discard tube or another sodium
citrate tube should be used first to purge the air from the tubing and allow a full draw into
the citrate tube. Purging the air from the tubing is also recommended before drawing any
additive tube. CLSI recommends that all additive tubes should be filled to their stated
volume.
• How much air is in the 12-inch and 7-inch tubing of the BD Vacutainer® Safety-
Lok™ Blood Collection Set and the BD Vacutainer® Push Button Blood Collection
Set?
The 12-inch tubing contains approximately 0.5mL of air and the 7-inch tubing contains
approximately 0.3mL of air.
• Does the BD Vacutainer® Push Button Blood Collection Set (PBBCS) contain latex?
No, the BD Vacutainer® Push Button Blood Collection Set is latex free.
• Does BD Preanalytical Systems have documentation about the safety efficacy of the
BD Vacutainer® Push Button Blood Collection Set (PBBCS)?
BD Preanalytical Systems has third party, peer reviewed articles that demonstrate that the
BD Vacutainer® Push Button Blood Collection Set reduces accidental needlesticks.
• What can I do if the audible click of the BD Vacutainer® Push Button Blood
Collection Set startles patients?
Let the patient know, prior to using the BD Vacutainer® Push Button Blood Collection
Set, that once they hear the audible click, the phlebotomy procedure is over.
• Where is the blood visualization or flash on the BD Vacutainer® Push Button Blood
Collection Set (PBBCS)?
The flash on the BD Vacutainer® Push Button Blood Collection set can be seen directly
behind and below the activation button.
• When should I activate the safety device on the BD Vacutainer® Push Button Blood
Collection Set (PBBCS)?
The BD Vacutainer® Push Button Blood Collection Set is designed to be activated while
the needle is still in the patient’s vein.
• What material is used to manufacturer the wings and tubing of the BD Vacutainer®
Push Button Blood Collection Set (PBBCS)?
The tubing of the BD Vacutainer® Push Button Blood Collection Set is made of
Polyvinyl Chloride and the wings are made of Polyolefin Elastomer material.
Eclipse Needle
• Why must the pink safety shield on the BD Vacutainer® Eclipse™ Blood Collection
Needle be gently rotated back toward the holder?
The pink shield is gently rotated back so it doesn't obstruct your view during
venipuncture and collection. The pink shield is attached to the needle to provide
protection from accidental needlesticks. The shield is directly in line with the bevel of the
needle and all collections are performed with the bevel in the "UP" position.
• Will there be any change in my collection technique when using BD Vacutainer®
Eclipse™ Blood Collection Needles?
No. The needle itself is the same BD Vacutainer® PrecisionGlide™ needle that you are
now using. The only difference is the added BD Eclipse™ safety shield, which provides
protection from accidental needlestick injuries.
• What material is used to manufacturer needles for BD PAS products?
The needles of the BD Vacutainer® Push Button Blood Collection Set, BD Vacutainer®
Safety-Lok™ Blood Collection Sets and BD Vacutainer® Eclipse™ Blood Collection
Needle are all made of stainless steel (grade 304).
Urine Collection
• Why use a BD Vacutainer® Urine Collection kit for urine collection when I can use
just a paper cup?
For simple dipstick urine tests performed at the collection site, a paper cup is acceptable.
For urine specimens that need to be transported to the lab, the BD Vacutainer® Urine
Collection Kit has a sterile interior. It is the closed system that allows fast, easy transfer
of urine from the cup to either a urinalysis tube or a culture and sensitivity tube (C&S),
and closed tube transport to the laboratory.
• What is the preservative in the BD Vacutainer® Culture and Sensitivity Tube (gray
top)?
The preservative in the BD Vacutainer® Culture and Sensitivity Tube (gray top) is a
combination of sodium formate, sodium borate and boric acid. The preservative helps to
preserve and protect the level of bacteria present at collection. It is mercury free.
• How long may the urine sample collected in the BD Vacutainer® Culture and
Sensitivity Tube be held before a culture is performed, and how long is this sample
stable at refrigerated temperatures?
The urine sample may be held at room temperature for 48 hours before test results are
compromised. Refrigeration will not extend the stability of the sample.
• What is the preservative in the BD Vacutainer® Plastic UA(Urinalysis) Preservative
Tube?
The preservative in the BD Vacutainer® UA Preservative tube is a combination of
Chlorhexidine, Ethyl Paraben and Sodium Propionate. It is a mercury free preservative
that meets the Environmental Protection Agency (EPA) and American Hospital
Associations’s requirements for a mercury free disposal environment.
• How long may the urine sample collected in the BD Vacutainer® Plastic UA
Preservative Tube be held before urinalysis testing?
The urine sample may be held at room temperature for 72 hours. Refrigeration will not
extend the stability time frame.
• Does the UA preservative have any effect on the KOVA system?
No, the preservative has been analyzed on the KOVA system for manual microscopy and
there were no effects on the physical characteristics of the sediment.
• Is the BD Vacutainer® Plastic UA Preservative Tube compatible with analyzers?
BD has done studies on various instruments.
The white papers may be accessed on the BD website at www.bd.com/vacutainer under
the section entitled “Library of Clinical Documentation”.
• Can the BD Vacutainer® Culture and Sensitivity Preservative Tube be used for
routine urinalysis testing?
BD does not recommend using the Culture and Sensitivity preservative tube for routine
urinalysis testing. Erroneous results may be seen for several of the chemistry parameters.
• Can the BD Vacutainer® Plastic UA Preservative Tube be used for pregnancy test?
BD has done studies using the BD Vacutainer® Plastic UA Preservative Tube for
pregnancy test. The white paper may be accessed on the BD website at
www.bd.com/vacutainer under the section entitled “Library of Clinical
Documentation”.
• Can the BD Vacutainer® Plastic UA Preservative Tube be used for urine drug
screens and culture and sensitivity tests?
BD does not recommend using the UA Preservative Tube for drug screen and culture and
sensitivity tests.
• What type of plastic is used to manufacture BD Vacutainer® urine collection tubes?
BD Vacutainer® urine collection tubes are made out of polyethylene terephthalate (PET)
plastic.
• Do you have a sterile urine transfer straw?
This product is not sterile but we have a sterile urine cup with integrated transfer device.
(#364975)
• Are your BD Vacutainer® Urine Collection Kits considered sterile?
Urine collection kits are not sterile. The cups and tubes have sterile interiors.
• Are the bulk-packed urine cups considered sterile?
These cups have sterile interiors.
• What is the total volume capacity for the BD Vacutainer® urine collection cup?
The BD Vacutainer® urine collection cup is a plastic cup graduated to indicate a volume
capacity of 4.5 oz or 120 mL of urine.
• How do I dispose of used urine specimen cup?
The blue lid is considered a sharp, and as such, needs to be disposed of in a sharps
container. Dispose of urine according to your facility’s policy and the collection cup in a
bio-hazard bag.
• What is the recommended order of draw for urine tubes?
Culture and sensitivity preservative tubes first and the urinalysis tubes second.
• Do you have any product that will collect urine from a Foley Catheter?
Yes, BD offers the BD Vacuatiner® Luer-Lok™ Access Device to be used with a 4 mL
C&S Preservative Tube and/or an 8 mL UA Conical Tube.
• What is the minimum volume of urine that should be collected into urine
preservative tubes?
The acceptable minimum draw volume for a BD Vacutainer® Plastic UA Preservative
Tube is 7 mL and for a BD Vacutainer® Culture and Sensitivity Preservative Tube is 3
mL.
Accessories
BD Vacutainer® Blood Transfer Device and BD Vacutainer® Luer-Lok Access Device
Molecular Diagnostics
The product inserts for these products are available on-line at:
http://www.bd.com/vacutainer/productinserts/
The PAXgene® Blood DNA System FAQ can be found on the PreAnalytiX website at
www.preanalytix.com/FAQ_DNA.asp
Regardless of the method of handling and shipping employed, always use the same
method for all studies, at all collection sites, in order to maintain baseline reproducibility