SAMPLING AND ANALYSIS OF SOAP AND SOAP PRODUCTS, VAR AOCS& Woe AOCS Official Method Da 14-48 Acid Value of Fatty Acids DEFINITION ‘The aid value isthe mg of potasium hydroxide (KOH) necessary to neutralize faty or rosin acids in 1 g ‘of sample (ee Notes, 1). SCOPE Applicable to fatty or rosin acids after separation from soap. Replaces Da 14-42. ‘APPARATUS 1. Erlenmeyer flask—250 ml. REAGENTS 1. Sodium hydroxide (NaOH) solution, 0.5 M—accuraely standardized. See AOCS Specification H 12-52 (see Notes, 1), 2. Ethyl aleohol—95%, SDA Formulas 30 and 3 are permitted (see Notes, Caution). Add 3-5 drops phenolphthalein indi ‘or solution. Boil and neutralize wit dilute sodium hydroxide (NaOH), 0.1-0.2 M solution, to faint pink color just before using. 3. Phenolphthalein indicator solution —1.0% in 95% alcohol PROCEDURE 1. The fatey acids are prepared as in Official Method, Da 12-48, Preparation, 1-5 2. Weigh accurately about 2g of fatty acids into 250 ml. Exlenmeyer flask and add 20-30 mL of hot, neutralized alcohol 3. Add 05 ml of indicator and tizate immediately while shaking with 0.5 N NaOH to the firs pink color which will persist for 30 se, CALCULATIONS Aci valu, mg KOF of fay aids = NAOH X M > 561 mass of test potion, g Where— ‘M = molarity of NaOH, NOTES Caution Ethyl alcohol («hanol) is flammable. A propetly operating fume hood should be used when heating or evaporating NUMBERED NOTES 1, Although standardized sodium hydroxide is used in che ttation, results are expressed in tems of mg KOH/g of fay aids This i taken into consideration in the calculation, nocing that 1 mL. of 1.0 M NaOH is equivalent co 56.1 mg of 1.0 M KOH. age 1 oft