Discussion/Interpretation

The intact protein is tested to identify and determine the functional groups that

they contain.

The Biuret test produces a blue violet solution due to the presence of a peptide

bonds.

The Ninhydrin test produces a blue violet solution also due to the reaction of the

free amino acid group and free carboxylic group with the ninhydrin solution.

The Xanthoproteic test produces a clear yellow solution due to the presence of

the side chains of aromatic amino acids.

The Millon’s test produces a brick red solution due to the tyrosine residues.

The Hopkins-Cole test produces a purple ring coloration at the interface due to

the presence of tryptophan residues.

The Sakaguchi test produces a red coloration due to the presence of guanidium

group (arginine); in alkaline solution it also gives a red coloration which contains a-

naphthol and sodium hypochlorite, but this test was not performed.

The Nitroprusside test produces a red coloration as well due to the presence of

cystein, the only amino acid containing a sulfhydryl group (-SH).

The Fohl’s test form an appearance of black sediment due to the presence of

sulfur containing amino acids.

The test for Amides shows the change of red litmus paper to blue due to the

presence of asparagine and glutamine.

 The Pauly test produces a red coloration due to the presence of tyrosine and

histidine residues. it involves the diazotization of sulphinicilic acid in the presence of

sodium nitrite and sodium carbonate, but this test was not performed.

Conclusion

From the obtained data the intact protein showed positive results in the Biuret

test, Ninhydrin test, Xanthoproteic test, Millon’s test, Hopkins-Cole test, Nitroprusside

test, Fohl’s test, and test for Amide. All positive result confirms the presence of proteins

or the specific functional group they contain. Though, the Sakaguchi test and the Pauly

test are not performed because of not having the solution or reagents, it is given that a

coloration in the solution should appear to identify the protein’s presence.