QUALITATIVE TEST TEST FOR REAGENT RESULT/S

- Biuret Reagent:
General test for the Hydrated CuSO4
BIURET TEST presence of peptide Potassium Hydroxide Blue-Violet Color
bonds Solution
Potassium Sodium
Tartrate

NINHYDRIN TEST For alpha-amino acids - Ninhydrin Solution Blue Violet Color

To detects the side

chains of aromatic
XANTHOPROTEIC TEST amino acids or to - Conc. HNO3 Clear Yellow
differentiate aromatic - Conc. NaOH Color
amino acids from
other amino acids

For detecting amino

acid containing - Millon’s Reagent Brick Red Color
MILLON’S TEST phenol group
(tyrosine)

For detecting amino - Hopkins-Cole Purple Ring

HOPKINS-COLE TEST acid tryptophan Reagent Coloration at the
present in protein - Conc. H2SO4 interface

For detecting - 10% NaOH

presence of - 0.02% Naphthol Red Color
SAKAGUCHI TEST guanidium group Solution
(arginine) - 2% NaOBr

For determining the

presence of cysteine, - 3M NaOH
NITROPRUSSIDE TEST the only amino acid - 2% Nitroprusside Red Color
containing a sulfhydryl Solution
group (-SH)
 For detecting the - 30% NaOH
FOHL’S TEST presence of sulfur - 5% Pb(CH3COO)2 Appearance of
containing amino Black sediment
acids

For detecting Red litmus paper

TEST FOR AMIDES asparagine and - 20% NaOH should turn to blue
glutamine

For detecting - Diazo Reagent:

PAULY’S TEST aromatic amino acid 1% Sulfanilic Acid & Red Color
tyrosine and histidine 5% NaNO2 Solution
- 10% Na2CO3