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  • Qualitative Test Test For: Reagent +result: University of Santo Tomas-Legazpi James G. Terrenal 2BSMT-1

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    The document summarizes several common qualitative tests used to identify different amino acid functional groups and peptides present in biological samples. It lists the name of each test, what biochemical substance or group it tests for, the reagents used in the test, and the expected positive result of the test indicated by a color change or precipitate formation. Some of the tests described include the Biuret test for peptides, the Ninhydrin test for amino groups, and specific tests for amino acid side chains like the Xanthoproteic test for tyrosine and tryptophan.

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    The document summarizes several common qualitative tests used to identify different amino acid functional groups and peptides present in biological samples. It lists the name of each test, what biochemical substance or group it tests for, the reagents used in the test, and the expected positive result of the test indicated by a color change or precipitate formation. Some of the tests described include the Biuret test for peptides, the Ninhydrin test for amino groups, and specific tests for amino acid side chains like the Xanthoproteic test for tyrosine and tryptophan.

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    © All Rights Reserved
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    7 views1 page

    Qualitative Test Test For: Reagent +result: University of Santo Tomas-Legazpi James G. Terrenal 2BSMT-1

    Uploaded by

    James Terrenal
    The document summarizes several common qualitative tests used to identify different amino acid functional groups and peptides present in biological samples. It lists the name of each test, what biochemical substance or group it tests for, the reagents used in the test, and the expected positive result of the test indicated by a color change or precipitate formation. Some of the tests described include the Biuret test for peptides, the Ninhydrin test for amino groups, and specific tests for amino acid side chains like the Xanthoproteic test for tyrosine and tryptophan.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 1

    University of Santo Tomas-Legazpi

    James G. Terrenal
    2BSMT-1

    Qualitative Test Test for: Reagent +Result


    Biuret Test General test for the Biuret reagent, Blue-violet color
    presence of peptide containing:
    bonds  Hydrated
    Copper
    sulphate
     Potassium
    hydroxide
    solution
     Potassium
    sodium
    tartrate
    Ninhydrin Test α-Amino group along Ninhydrin Violet complex
    with amino group (triketohydrindene (Ruhemann’s Purple)
    nitrogen atom hydrate)
    Xanthoproteic Test Substituted aromatic Concentrated HNO3 Yellow-orange ppt
    ring of Tyr, Trp (and
    Phenylalanine)
    Millon’s Test Phenolic ring of Millon’s Reagent = Old rose (Pale Red) ppt
    Tyrosine HgSO4 (Mercuric) in
    H2SO4
    Hopkins-Cole Test Indole group of Glyoxilic acid (glacial Violet
    Tryptophan acetic acid exposed to
    sunlight)+ Reaction w/
    H2SO4
    Sakaguchi Test Guanidium group of α-naphthol (1-hydroxy Red-Orange Complex
    Arginine naphthalene)
    Nitroprusside Test SH of Cysteine Sodium Nitroprusside Red complex
    Fohl’s Test
    Test for Amides
    Pauly’s Test Tryptophan or PaulySulphanilic acid + Red (Azo dye)
    Histidine HCl

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