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Science
Quarter 3 – Module 4.1:
Protein Synthesis
Science – Grade 10
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Quarter 3 – Module 4.1: Protein Synthesis
First Edition, 2020
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Science
Quarter 3 – Module 4.1:
Protein Synthesis
Introductory Message
This Self-Learning Module (SLM) is prepared so that you, our dear learners, can
continue your studies and learn while at home. Activities, questions, directions,
exercises, and discussions are carefully stated for you to understand each lesson.
Each SLM is composed of different parts. Each part shall guide you step-by-step as
you discover and understand the lesson prepared for you.
Pre-tests are provided to measure your prior knowledge on lessons in each SLM. This
will tell you if you need to proceed on completing the module or if you need to ask
your facilitator or your teacher’s assistance to better understanding of the lesson. At
the end of each module, you need to answer the post-test to self-check your learning.
Answer keys are provided for each activity and test. We trust that you will be honest
in using these.
In addition to the material in the main text, Notes to the Teacher are also provided
to our facilitators and parents for strategies and reminders on how they can best
help you on your home-based learning.
Please use this module with care. Do not put unnecessary marks on any part of this
SLM. Use a separate sheet of paper in answering the exercises and tests. And read
the instructions carefully before performing each task.
If you have any questions in using this SLM or any difficulty in answering the tasks
in this module, do not hesitate to consult your teacher or facilitator.
Thank you.
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What I Need to Know
This module was designed and written with you in mind. It is here to help you
master the role of DNA and RNA on the processes of protein synthesis. The scope of
this module permits it to be used in many different learning situations. The language
used recognizes the diverse vocabulary level of students. The lessons are arranged
to follow the standard sequence of the course. But the order in which you read them
can be changed to correspond with the textbook you are now using.
What I Know
Directions: Read each item carefully. Choose the letter of the best answer and write
it in your notebook/on a separate sheet of paper.
2. Which of the following contains the bases adenine, thymine, guanine and
cytosine?
a. DNA
b. mRNA
c. RNA
d. tRNA
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3. What kind of sugar does RNA have?
a. Deoxyribose
b. Galactose
c. Lactose
d. Ribose
9. Which is the mRNA molecule that would be transcribed from this DNA
template TGG CAA GTA CGT?
a. ACC GTT CAT GCA
b. ACC GUU CAU GCA
c. UCC GUU CUU GCU
d. UGG CAA GUA CGU
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10. What would be the tRNA anticodon for the mRNA codon CAG?
a. CAG
b. CUG
c. GTC
d. GUC
Lesson
Structure and Function of
1 DNA and RNA
Nucleic Acids are important macromolecule in the cell because of their role
in the transmission, storage and expression of genetic information. The two major
types of nucleic acids are Deoxyribonucleic Acid (DNA) and Ribonucleic Acid
(RNA). They both differ in terms of the composition, role and location in the cell.
What’s In
Directions: Label the parts of the chromosome. You can choose from the pool of
words below. Write your answers in your notebook/on a separate sheet of paper.
3.
4.
1.
5.
2.
3
What’s New
Directions: Encircle the given words in the grid, running in different possible
directions horizontally, vertically, or diagonally. Words are written in the word pool
below.
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What is It
Study the table below to understand the differences of DNA and RNA.
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Figure 2: Chemical Structure of DNA
DNA and RNA are strings of nucleotides which are composed of phosphate
group, deoxyribose sugar for DNA while ribose sugar for RNA and nitrogenous
base. There are two kinds of nitrogen containing bases – the Purines and
Pyrimidines. Purines (Adenine and Guanine) are both present in DNA and RNA and
have two-ring structure while pyrimidines in DNA are Cytosine and Thymine, in RNA
are Cytosine and Uracil have only one ring. Nitrogen bases are held together by
hydrogen bonds.
The function of DNA is primarily for the storage of genetic information while
RNA converts information stored in the DNA to form protein. The types of RNA
involved in protein synthesis are the mRNA (messenger RNA), tRNA (transfer RNA)
and rRNA (ribosomal RNA). Messenger RNA carries information from the DNA in the
nucleus to the site of protein synthesis while the Transfer RNA supplies the amino
acids to the ribosome to form proteins, and Ribosomal RNA binds tRNA and various
molecules necessary for protein synthesis.
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What’s More
Directions: Below are the characteristics found in either DNA or RNA or both. Put a
check (✔) mark on the appropriate column if the given characteristic is found in
DNA or RNA. Write your answers in your notebook/on a separate sheet of paper.
I. Directions: Write the complementary pair for each given DNA strand. Write your
answers on separate sheet of paper.
1. CGT AAG CGC TAA TTA 2. ACT AAC GGT AGC TAG
3. AAT GAA TAG CTA GCT 4. CTT GGC TTA TGG TGG
5. CGT TAG CAT GCT TCA
II. Direction: Write the mRNA strand for the given DNA strand.
6. CGT AAG CGC TAA TTA 7. ACT AAC GGT AGC TAG
8. AAT GAA TAG CTA GCT 9. CTT GGC TTA TGG TGG
10. CGT TAG CAT GCT TCA
1. DNA (Deoxyribonucleic Acid) is a double stranded helix nucleic acid that stores
genetic information of living organisms. It is located inside the nucleus and some
can be found in mitochondria.
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2. RNA (Ribonucleic Acid) is a single stranded nucleic acid that converts stored
genetic information to proteins known as the process of protein synthesis. RNA
forms in the nucleolus, and then moves to specialized regions of the cytoplasm
depending on the type of RNA formed.
4. The complementary base pairs in DNA are Adenine (A) – Thymine (T) and Cytosine
(C) – Guanine (G) while in RNA Thymine replaces Uracil (U).
What I Can Do
Source: https://www.biologycorner.com/2017/03/18/construct-a-dna-model/
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Assessment
Directions: Choose the letter of the best answer and write it in your notebook/on a
separate sheet of paper.
1. Which of the following is the complementary strand of CGT TTA?
a. GCA AAT
b. GCC AAT
c. GCU CCT
d. UUA AAT
5. Between the two strands of a DNA segment, the nitrogen bases are held
together by _________.
a. covalent bonds
b. ionic bonds
c. hydrogen bonds
d. metallic bonds
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7. The DNA molecule is _________ stranded and the RNA molecule is single
stranded.
a. Double
b. Quadruple
c. Single
d. Triple
8. Purines are both present in DNA and RNA and have _________ ring structure.
a. one
b. four
c. three
d. two
Additional Activities
Directions: Complete the Venn diagram below by writing down the differences and
similarities of DNA and RNA. Write your answers in your notebook/on a separate
sheet of paper.
1. Single stranded
2. Contains deoxyribose sugar
3. Important for protein synthesis
4. Stores genetic information
5. Adenine pairs with Thymine
6. Uses genetic information for
protein synthesis
7. Contains Uracil
8. Can be found in mitochondria
9. Cytosine pairs with Guanine
10. Contains ribose sugar
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Lesson
2 Protein Synthesis
Protein is the most important molecule in the cell. Enzymes, antibodies and
some hormones are examples of protein that play an important role in living
organisms. The production of proteins is called Protein Synthesis which consists of
two processes – transcription and translation.
Now, let us discover how proteins are formed.
What’s In
Before we proceed to the processes of protein synthesis, let us review the two
important molecules involved in the formation of proteins.
Directions: Complete the table below. Write your answers in your notebook/on a
separate sheet of paper.
DNA RNA
Composition
Function
Base pairing
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What’s New
Across: Down:
2. This refers to the trinucleotide 1. It is the process in which the
sequence of DNA or RNA. genetic code in mRNA is read to
4. It is the process by which the make a protein.
instructions in DNA are converted 3. It is the process by which the
into a functional product. information of DNA is copied into
7. It is the beginning stage of mRNA.
transcription. 5. It is the complementary code to the
codon for an amino acid.
6. It refers to the building block of
proteins.
What is It
Proteins are large complex molecules that are essential for normal
functioning in the cell. Proteins are made up of smaller amino acids, which are
building blocks of proteins. Hundreds of amino acids are linked together by peptide
bonds forming a long chain of proteins.
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There are 20 amino acids which are classified based on their polarities and
charge. Each amino acid is also coded by DNA in a trinucleotide sequence or in
groups of three bases which refers as codon. Refer to the Amino Acid Chart below.
THIRD LETTER
FIRST LETTER
1. Transcription
This process involves the transfer of genetic information in DNA to mRNA
(messenger RNA) which happens inside the nucleus. This takes place in
three steps: initiation, elongation and termination.
a. Initiation is the beginning of transcription whereas the enzyme RNA
polymerase binds to a region of a gene called promoter. After this, the
DNA unwinds so that the enzyme can read the bases of the DNA strand.
b. Elongation refers to the addition of nucleotides to the mRNA.
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c. Termination is the ending of transcription and the mRNA detaches to
the DNA.
Processing of mRNA
After the termination stage, the new mRNA is not yet ready for translation, it
must undergo more processes before it leaves the nucleus. These processes are the
following:
• Splicing – refers to the removal of introns in the mRNA. Introns are the non-
coding regions while the coding regions are called Exons.
• Editing – modification or change some of the nucleotides in mRNA.
• Polyadenylation - adds a tail to the mRNA. The tail consists of a string of
adenine bases. It signals the end of mRNA. It is also involved in exporting
mRNA from the nucleus, and it protects mRNA from enzymes that might break
it down.
2. Translation
It is the process in which the genetic code in mRNA is read to make a
protein. After mRNA leaves the nucleus, it moves to a ribosome, which
consists of rRNA and proteins. The ribosome reads the sequence of codons in
mRNA, and molecules of tRNA bring amino acids to the ribosome in the correct
sequence.
In order to start the translation process, the following molecules are
required: mRNA template, ribosomes, tRNA, and various enzymatic factors.
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Figure 4: Ribosome
Amino Acid
Just like the transcription, this process takes place in three steps:
initiation, elongation and termination.
a. Initiation – transitional complex forms and tRNA brings the first amino
acid (Methionine) in polypeptide chain to bind to start codon on mRNA.
b. Elongation – tRNAs bring amino acids one by one to add to polypeptide
chain.
c. Termination – this happens when a stop codon in the mRNA (UAA, UAG,
or UGA) enters the A site.
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In the initiation stage, the
tRNA carrying amino acid
methionine with anticodon
AUG binds to the start
codon on mRNA. This event
occurs on the P-site of the
ribosomes.
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The ribosome shifts down by a distance of
one codon. As the shift occurs, the two
amino acids on the tRNA on the P-site are
transferred to the new amino acid and the
second tRNA is released from the E-site.
Directions: Use the codon chart below to write the amino acid and codon that
correspond to each item. Write your answers in your notebook/on a separate sheet
of paper.
SECOND LETTER
A U C G
AAA Lysine AUA Isoleucine ACA AGA Arginine A
(Lys) (Ile) (Arg)
AAU Asparagine AUU Isoleucine ACU AGU U
Serine
(Asn) Threonine
A (Ser)
AAC Asparagine AUC Isoleucine ACC (Thr) AGC C
AAG Lysine AUG Start codon; ACG AGG Arginine G
Methionine
(Met)
UAA Stop codon UUA Leucine (Leu) UCA UGA Stop codon A
UAU Tyrosine UUU Phenylalanine UCU UGU U
Serine Cysteine
(Tyr) (Phe)
THIRD LETTER
FIRST LETTER
U (Cys)
UAC Tyrosine UUC Phenylalanine UCC (Der) UGC C
UAG Stop codon UUG Leucine (Leu) UCG UGG Tryptophan G
(Trp)
CAA Glutamine CUA CCA CGA A
(Gln)
CAU Histidine CUU CCU Proline CGU U
C Leucine Arginine
(His) (Pro)
CAC Histidine CUC CCC CGC C
CAG Glutamine CUG CCG CGG G
GAA Glutamic GUA GCA GGA A
acid (Glu)
GAU Aspartic GUU GCU GGU U
acid (Asp) Alanine Glycine
G Valine (Val)
GAC Aspartic GUC GCC (Ala) GGC (Gly) C
acid
GAG Glutamic GUG GCG GGG G
acid
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1. Aspartic acid 6. GAC 1. AUC
2. Glutamic acid 7. GCG 2. AAA
3. Valine 8. UUU 3. GUG
4. Glycine 9. GUU 4. CCC
5. Methionine 10. GGG 5. AAC
Directions: For each sequence of DNA is shown. Write the complementary RNA
sequence underneath the letters, then use the codon chart to determine the amino
acid sequence. Write your answers in your notebook/ on a separate sheet of paper.
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What I Can Do
DNA
mRNA
tRNA
Amino
acids
Source: https://www.biologycorner.com/2017/03/18/construct-a-dna-model/
Assessment
Directions: Choose the letter of the best answer and write it in your notebook/on a
separate sheet of paper.
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3. Which of the following is the start codon?
a. AAG
b. AUG
c. CCU
d. GAG
4. What is the sequence of amino acids produced by this mRNA UUA GCG CCG
AAA AUA?
a. Asn, Arg, Gly, Phe, Tyr
b. Ile, Phe, Asn, Lys, Ser
c. Leu, Lys, Phe, Gln, Asn
d. Pro, Leu, Lys, Thr, Ala
6. What is the sequence of amino acids produced by this DNA sequence GGA
GTT TTC?
a. Glycine, Glutamic acid, Leucine
b. Glycine, Valine, Leucine
c. Proline, Glutamine, Lysine
d. Proline, Valine, Lysine
7. If the code for an amino acid is AGC on the DNA molecule, the anticodon on
the tRNA would be ________?
a. AGC
b. TGC
c. UCG
d. UGC
8. Which of the following would not occur during complementary base pairing?
a. A-T
b. A-U
c. C-G
d. U-G
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10. During protein synthesis, peptide bonds are formed at the________.
a. Lysosomes
b. Nucleus
c. Nucleolus
d. Ribosomes
Additional Activities
Directions: Create a model of transcription using the following models below then
answer the guide questions. Use a separate sheet of paper in pasting the models.
Source: https://www.biologycorner.com/2017/03/18/construct-a-dna-model/
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Guide Questions:
1. What are the anticodons of the following codons?
GGU AUC GUU GAA
CAG UGU UGC GCU
UCC GUG UGC CUG
UAC CAA CUA GAA
AAC UAC UGC AAU
UAA
2. Starting from the left, write the sequence of the amino acids formed by
translation of the mRNA strand.
_____________________________________________________________________
____________________________________________________________________
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Activity 1.2 Lesson 1:
1. GCA TTC GCG ATT AAT What I Know
2. TGA TTG CCA TCG ATG 1. d
3. TTA CTT ATC CAT CGA 2. a
4. GAA CCG AAT ACG ACC 3. a
5. GCA ATC GTA CGA AGT 4. d
6. GCA UUC GCG AUU AAU 5. a
7. UGA UUG CCA UCG AUC 6. a
8. UUA CUU AUC GAU CGA 7. d
9. GAA CCG AAU ACC ACC 8. a
10. GCA AUC GUA CGA AGU 9. c
Assessment: 10. d
1. a What’s In
2. b
1. Centromere
3. a
2. DNA
4. a
3. Telomere
5. c
4. p arm
6. d 5. q arm
7. a
8. d
What’s New
9. c
10. d
Additional Activities:
What’s More
Lesson 2: Activity 1.1
What’s In
Answer Key
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What’s New Assessment:
1. Translation 1. a
2. Codon 2. b
3. Transcription 3. b
4. Central Dogma 4. a
5. Anticodon 5. b
6. Amino acids 6. b
7. Initiation 7. c
What’s More 8. d
Activity 2.1 9. b
1. GAU, GAC 10. d
2. GAA, GAG
3. GUA, GUU,GUC, GUG
4. GGA, GGU, GGC, GGG
5. AUG
6. Aspartic acid
7. Alanine
8. Phenylalanine
9. Valine
10. Glycine
11. Isoleucine
12. Lysine
13. Valine
14. Proline
15. Asparagine
What’s More
Activity 2
DNA TAC CAT GGA AAT ATT
mRNA AUG GUA CCU UUA UAA
tRNA UAC CAU GGA AAU AUU
Amino acid Tyr His Gly Asn Ile
DNA TTC AAT GGT CTA GGG
mRNA AAG UUA CCA GAU CCC
tRNA UUC AAU GGU CUA GGG
Amino acid Phe Asn Gly Leu Gly
DNA ACA TTT CAG ACC GTC
mRNA UGU AAA GUC UGG CAG
tRNA ACA TTT CAG ACC GUC
Amino acid Thr Phe Gln Asn Val
What I Can Do
Copy of the Learner
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Copy of the Learner
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References
Lilia Rabago, Functional Biology Modular Approach: (1253 G. Araneta Ave., Quezon
City), pp. 45-50
Pablo Belardo, Conceptual Science and Beyond Biology II: (Bonanza Plaza 2,Block 1
Lot 6 Hilltop Subdivision Greater Largo, Novaliches, Quezon City, 2010), p. 32
https://www.biologycorner.com/2017/03/18/construct-a-dna-model/
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