Insulin & Glucose Signaling

Insulin Receptor
SNARE TNF
Glucose Complex
GLUT4
p85

Gab1
FlotillinCav

TNFR1
PTEN PI3K
CAP

Synip Cbl p110 SOCS3

IRS

Shc
PIP3
APS SHIP Akt2 SHP-2 PTP1B
Crkll

GLUT4 EHD1 GRB2 Nck

Translocation TC10 C3G
EHBP1 IRS-1 Crkll
CIP4/2 PKCλ/ζ Fyn SOS
PDK1
Rac1 mTORC2
PKCθ GRB10
Jnk
GLUT4 PKCλ/ζ IKK
vesicle Ras
PP2A FFA
14-3-3 LKB1 NO iNOS
GLUT4 AS160 Akt
Exocytosis ROS c-Raf
TBC1D1 AMPK
PDK1 14-3-3
TSC2 PDE3B
TSC1
Bad SIK2
GSK-3 PRAS40 [cAMP]
Rheb MEK1/2
SGK
Apoptosis SREBP CBP/p300
mTORC1 PKA Torc2
p70 Erk1/2
PP1 GS Lipin1 4E-BP1 S6K
ATP-citrate Degradation
ENaC lyase eIF4E HSL
Glycogen
Synthesis Protein Synthesis,
Fatty Acid Growth, and SREBP-1
Sodium Synthesis Proliferation Lipolysis
Transport

lasm
Cytop Erk1/2
SGK Akt2 Fatty Acid
us and Cholesterol Disruption of
Nucle Synthesis CBP/Torc2/CREB
Complex
FoxO3 FoxO4 FoxO1 Erk1/2
SREBP LXRα USF Gluconeogenesis
Transcription Apoptosis,
mTORC1 mTORC2 Autophagy, Lipin1
Raptor Sin1 PRR5
Glucose and
GβL
mTOR Rictor
Lipid Metabolism Growth
GβL
DEPTOR
mTOR
DEPTOR

For pathway key and background please visit:

www.cellsignal.com/insulin
 Key Products for Insulin & Glucose Signaling
Visit www.cellsignal.com/insulinlist for full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY ANTIBODIES APPLICATIONS REACTIVITY

#9644 4E-BP1 (53H11) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk #13120 iNOS (D6B6S) Rabbit mAb WB, IP, IF-IC, F M
#4691 Akt (pan) (C67E7) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk, Dm, (Pg) #4370 Phospho-p44/42 MAPK (Erk1/2) WB, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, Mi, Dm,
#4060 Phospho-Akt (Ser473) (D9E) XP® WB, IP, IHC-P, IHC-F, H, M, R, Hm, Mk, Dm, Z, B, (Thr202/Tyr204) (D13.14.4E) XP® Z, B, Dg, Pg, Sc, (C)
Rabbit mAb IF-IC, F (C, X, Dg, Pg) Rabbit mAb
#3063 Akt2 (D6G4) Rabbit mAb WB, IP H, M, R, Mk #2708 p70 S6 Kinase (49D7) Rabbit mAb WB, IHC-P H, M, R, Mk
#8599 Phospho-Akt2 (Ser474) (D3H2) WB, IP H, M, R #9234 Phospho-p70 S6 Kinase (Thr389) WB H, M, R, Mk, (C)
Rabbit mAb (Akt2 Specific) (108D2) Rabbit mAb
#5832 AMPKα (D63G4) Rabbit mAb WB, IP H, M, R, Mk, B #12940 PCK1 (D12F5) Rabbit mAb WB H, M, R, Mk
#2535 Phospho-AMPKα (Thr172) (40H9) WB, IP, IHC-P H, M, R, Hm, Mk, Dm, Sc, #5662 PDK1 (D37A7) Rabbit mAb WB H, M, R, Hm, Mk
Rabbit mAb (C, Z, B, Pg) #3438 Phospho-PDK1 (Ser241) (C49H2) WB, IP H, M, R
#2670 AS160 (C69A7) Rabbit mAb WB, IP H, M, R Rabbit mAb
#11815 ATF-4 (D4B8) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R #4249 PI3 Kinase p110α (C73F8) WB, IP, IHC-P H, M, R, B
#7389 CBP (D6C5) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk Rabbit mAb
#12311 DNA-PK (3H6) Mouse mAb WB, IHC-P, IF-IC H, Mk #9368 PKCζ (C24E6) Rabbit mAb WB H, M, R, Mk
#2067 eIF4E (C46H6) Rabbit mAb WB, IP, IHC-P H, M, R, Mk #7067 PKM1 (D30G6) XP® Rabbit mAb WB, IHC-P, IF-F, IF-IC H, M
#3180 Fatty Acid Synthase (C20G5) WB, IP, IHC-P, IHC-F, IF-IC H, M, R, (B) #4053 PKM2 (D78A4) XP® Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R, Mk
Rabbit mAb #2691 PRAS40 (D23C7) XP® Rabbit mAb WB, IP, IHC-P H, M, R, Mk
#2880 FoxO1 (C29H4) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk #2997 Phospho-PRAS40 (Thr246) (C77D7) WB, IP, IHC-P H, M, R, Mk
#12829 FoxO3a (D19A7) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R Rabbit mAb
#12939 Glut1 (D3J3A) Rabbit mAb WB, IP H, M, R #9188 PTEN (D4.3) XP® Rabbit mAb WB, IP, IHC-P H, M, R, Mk, Dg, (C)
#2213 Glut4 (1F8) Mouse mAb WB H, M, R #9549 Phospho-PTEN (Ser380/ WB H, M, R, Mk, (C)
Thr382/383) (44A7) Rabbit mAb
#3886 Glycogen Synthase (15B1) WB, IP, IHC-P H, M, R
Rabbit mAb #2280 Raptor (24C12) Rabbit mAb WB, IP H, M, R, Mk
#12456 GSK-3β (D5C5Z) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk #13879 Rheb (E1G1R) Rabbit mAb WB, IP H, M, R, Mk
#2024 Hexokinase I (C35C4) Rabbit mAb WB, IP, IHC-P, IF-IC H, M #9476 Rictor (D16H9) Rabbit mAb WB H, M
#2867 Hexokinase II (C64G5) Rabbit mAb WB H, M, R, Mk #3806 Phospho-Rictor (Thr1135) (D30A3) WB H, M, Mk
Rabbit mAb
#18381 HSL (D6W5S) XP® Rabbit mAb WB, IP, IF-IC H, M
#11998 SDHA (D6J9M) XP® Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R, Hm, Mk
#9750 IGF-I Receptor β (D23H3) XP® WB, IP, IF-IC, F H, M, R, Mk
#3397 SHP-2 (D50F2) Rabbit mAb WB, IP H, M, R, Mk
Rabbit mAb
#6919 SIK2 (D28G3) Rabbit mAb WB, IP H, M, (R)
#3014 Insulin (C27C9) Rabbit mAb IHC-P, IF-F, IF-IC, F H, M, R
#2429 Thioredoxin 1 (C63C6) Rabbit mAb WB, IHC-P H, M, R
#3025 Insulin Receptor β (4B8) WB, IP H, M, R
Rabbit mAb #4308 Tuberin/TSC2 (D93F12) XP® WB, IP, IF-IC, F H, M, R, Hm, Mk
Rabbit mAb
#2382 IRS-1 Antibody WB, IP H, M, R
#14715 TXNIP (D5F3E) Rabbit mAb WB, IP H, M, R, Mk
#3047 LKB1 (D60C5) Rabbit mAb WB H, M, R, Mk
#3482 Phospho-LKB1 (Ser428) (C67A3) WB H, M, R, Mk
Rabbit mAb RELATED KITS AND REAGENTS
#2195 Mnk1 (C4C1) Rabbit mAb WB H, M #12879 IRS-1 Inhibition Antibody Sampler Kit
#2983 mTOR (7C10) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Mk, (Hr) #7135 PathScan® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit
#5536 Phospho-mTOR (Ser2448) (D9C2) WB, IP, IF-IC H, M, Mk, (R, C, Pg, Hr) #7258 PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Kit
XP® Rabbit mAb #7177 PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit

Phospho-AMPKα kDa
200
(Thr172) (40H9) 140
100
Rabbit mAb #2535: 80 Phospho-
WB analysis of extracts 60 AMPKα
(Thr172)
from C2C12 cells, 50

untreated or treated 40

with oligomycin #9996 30

(0.5 uM), using #2535
(upper) and AMPKα 200
140
Antibody #2532 100
80
(lower).
60 AMPKα
50

40
IGF-I Receptor β (D23H3) XP® Rabbit mAb Insulin (C27C9) Rabbit mAb #3014: IHC Phospho-Akt (Ser473) (D9E) XP® Rabbit
30 #9750: Confocal IF analysis of MCF7 cells using analysis of paraffin-embedded human pancreas, mAb #4060: Confocal IF analysis of insulin-
– + Oligomycin #9750 (green). Blue pseudocolor = DRAQ5® showing the staining of β cells, using #3014. treated C2C12 cells using #4060 (green). Actin
#4084 (fluorescent DNA dye). filaments have been labeled with Alexa Fluor®
555 phalloidin #8953 (red). Blue pseudocolor =
DRAQ5® #4084 (fluorescent DNA dye).
16FLYCELM0040ENG

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology

© 2016 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, PathScan, and XP are all trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

For Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/insulin