Tabel 1 Susunan Primer yang Digunakan dalam Penelitian ini

Nama
Susunan (5’-3’) Pasangan Produk Sumber
Primer
Semua gen
Uni12: AGCAAAAGCAGG   WHO 2002
influenza A
HA-Reverse
HA-1144: GGAATGATAGATGGNTGGTAYGG HA 5, 6, dan 9 WHO 2002
(600 bp)
HA- ATATCGTCTCGTATTAGTAGAAACAAGG
    WHO 2002
Reverse: GTGTTTT
H5-1735R : GTGTTTTTAAYTAMAATCTGRACTMA HA1144 HA H5 WHO 2002
H6-1480R: AATTCAAAGCAACCATTCCC HA1144 HA H6 WHO 2002
H9-1: AGCAAAAGCAGGGGAAYWWC H9-808R HA H9 WHO 2002
H9-808R: CCATACCATGGGGCAATTAG   HA H9 WHO 2002

M-WSN-8: GAAGGTAGATATTGAAAGATG M-1023R MA influenza A WHO 2002

M-1023R: GAAACAAGGTAGTTTTTTACTC     WHO 2002
CU-MF: TGATCTTCTTGAAAATTTGCAG CU-MR HA H5 Payungporn et al, 2004
CU-MR: TGTTGACAAAATGACCATCG     Payungporn et al, 2004
CU-H5F: GACTCAAATGTCAAGAACCTTTA CU-H5R HA H5 Payungporn et al, 2004
CU-H5R: CCACTTATTTCCTCTCTGTTTAG     Payungporn et al, 2004
CU-N1F GTTTGAGTCTGTTGCTTGGTC CU-N1R NA N1 Payungporn et al, 2004
CU-N1R: TGATAGTGTCTGTTATTATGCC     Payungporn et al, 2004
AN1B : TTGCTTGGTCAGCAAGTGCA 708 bp NA N1 Takao et al, 2002
AN1D2 TTAGCTCAGGATGTTGAACG   NA N1 Takao et al, 2002
AN2B : GGTGACGAGAGAACCTTATG 615 bp NA N2 Takao et al, 2002
AN2C2: CCTGAGCACACATAACTGGA   NA N2 Takao et al, 2002
H5-1: GCC ATT CCA CAA CAT ACA CCC 219 bp HA H5 WHO 2005
H5-3: CTC CCC TGC TCA TTG CTA TG HA H5 WHO 2005
N1-1 TTG CTT GGT CGG CAA GTG C 616 bp NA N1 WHO 2005
N1-2 CCA GTC CAC CCA TTT GGA TCC NA N1 WHO 2005
H9-426: GAA TCC AGA TCT TTC CAG AC 383 bp HA H9 WHO 2005
H9-808R: CCA TAC CAT GGG GCA ATT AG HA H9 WHO 2005
NDVF GGTGAGTCTATCCGGARGATACAAG 202 bp ND F Creelan et al, (2002)
NDVR TCATTGGTTGCRGCAATGCTCT ND F Creelan et al, (2002)

Keterangan: W = (AT); Y = (CT); N = (AGTC); M = (AC); MA = matriks; HA:

hemaglutinin; NA: neuraminidase; ND F: protein Fusi virus Newcastle Disease

Primer Matriks yang lain

HI-F GGCATTTTGGACAA HI-E 243 Matri Prof. M.
AKCGTCTA bp x Peiris
influe Personal
 nza A Comm
Matri Prof. M.
CTTCTAACCGAGGT 243 x Peiris
HI-E HI-F
CGAAACG bp influe Personal
nza A Comm

Primer Universal Avian Influenza

Bm-HA-1
TATTCGTCTCAGGGAGCAAAAGCAGGGG
BM-NS-HA-890R
ATATCGTCTCGTATTAGTAGAAACAAGGGTGTTTT
Ba-NA-1
TATTCGTCTCAGGGAGCAAAAGCAGGAGT
Ba-NA-1413R
ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTTT
Bm-NS-1
TATTCGTCTCAGGGAGCAAAAGCAGGGTG
Bm-N3-1
TATTCGTCTCAGGGAGCAAAGCAGGTGC
Bm-N3-1420R
ATATCGTCTCGTATTAGTAGAAACAAGGTGCTTTTT
Bm-N6-1
TATTCGTCTCAGGGAGCAAAAGCAGGGTGAAAATG
Bm-N7-1
TATTCGTCTCAGGGAGCAAAAGCAGGGTGATTGAGAATG
Bm-N9-1
TATTCGTCTCAGGGAGCAAAAGCAGGGTC
Bm-N9-1473R
ATATCGTCTCGTATTAGTAGAAACAAGGGTCTT