Products for

the Pur ification

of Nucleic Acids
Selection guide

18-1148-07 AB
New GFX 96
GFX 96 Polymerase Chain Reaction (PCR) Purification Kit utilizes glass-fibre matrix technology in a
96-well format for the efficient purification of up to 96 PCR products simultaneously. The high yields
of purified DNA are ready for use in a variety of molecular applications, including microarrays, fluorescent
sequencing, labelling, hybridization, ligation, and transformation.

● Filter plates, collection plates, wash plates, and
buffered solutions are all provided in one box
● The 96-well plate is compatible with most
common 96-well plate-adapted centrifuges and
vacuum manifolds
● No hazardous organic extractions or ethanol
precipitation are required to isolate purified DNA
● The protocol has been optimized for customer
convenience with 1-96 PCR products purified
in less than 20 minutes
● Typical recoveries are > 90% for PCR
fragments 100–2000 base pairs in length, and
salt removal is typically > 99.99%

New AutoSeq 96
AutoSeq™96 Dye Terminator Clean-up Kit has been specifically designed for rapid removal of
unincorporated fluorescent dye terminators and other low molecular weight impurities from sequencing
reactions prior to analysis on automated sequencers.

● Automated filling process with high accuracy ● Removes >99.5% unincorporated ddNTPs
● Economical in 15 minutes
● From the makers of Sephadex™
● Stable at room temperature (2 months) and
+4°C (6 months)
● Average 50,000 bases per plate
● Long read lengths
● Simple protocol
 Products for the Purification of:
Product Name Major Subsequent Applications
mRNA QuickPrep™ Micro mRNA RT-PCR, Northerns, cDNA
Purification Kit (27-9255-01) synthesis, in vitro translation
QuickPrep mRNA Northerns, cDNA synthesis,
Purification Kit (27-9254-01) in vitro translation, RT-PCR
mRNA Purification Kit (27-9258-01, -02) Northerns, cDNA synthesis, in vitro translation, RT-PCR
Total RNA QuickPrep Total RNA Extraction Kit (27-9271-01) Northerns, mRNA purification, RT-PCR
RNA Extraction Kit (27-9270-01) Northerns, mRNA purification, RT-PCR
Caesium Trifluoroacetate (17-0847-02) Northerns, mRNA purification, RT-PCR
Labelled DNA ProbeQuant™ G-50 Micro Columns (27-5335-01) Hybridization
MicroSpin™ G-50 Columns Automated sequencing
(27-5330-01, -02)
AutoSeq™ G-50 (27-5340-01,-02,-03) Automated sequencing
PCR* Products GFX™ 96 PCR Purification Kit Labelling, automated and manual
(25-6902-01, 25-6909-02) sequencing, restriction cloning
GFX PCR DNA and Labelling, automated and manual
Gel Band Purification Kit (27-9602-01) sequencing, restriction cloning
MicroSpin G-25 Columns (27-5325-01) Restriction, sequencing
MicroSpin S-300 HR Columns (27-5130-01) PCR, restriction, sequencing
MicroSpin S-400 HR Columns (27-5140-01) PCR, restriction, sequencing
Sephaglas™ BandPrep Kit (27-9285-01) Sequencing, restriction, cloning
Genomic DNA Genomic Prep™ Blood DNA Isolation Kit (27-5237-01) PCR, sequencing, restriction mapping, Southerns
Genomic Prep Cells and Tissue Isolation Kit (27-5236-01) PCR, sequencing, restriction mapping, Southerns
GFX Genomic Blood Purification Kit (27-9603-01) PCR, sequencing, restriction mapping, Southerns
Nucleon™ BACC (RPN 8501/ 8502/ 8512) PCR, sequencing, restriction mapping, Southerns

Fig 1. mRNA prepared from very

small samples, then PCR-amplified Nucleon HT (RPN 8509) PCR, sequencing, restriction mapping, Southerns
following conversion to cDNA.
Panel A: Rabbit (-globin cDNA. Nucleon PhytoPure™ (RPN 8510/ 8511) PCR, RAPDs, restriction mapping, Southerns, AFLP
mRNA from a single rabbit
reticulocyte was purified using NAP™ Columns (17-0852, Sequencing, PCR, labelling
QuickPrep mRNA Purification Kit,
then reverse-transcribed using 17-0853, 17-0854; all -01, -02)
First-Strand cDNA Synthesis Kit
(27-9261-01) and pd(N)6 as MicroSpin G-25 Columns (27-5325-01) Hybridization, sequencing, PCR
primer.The entire reaction was
amplified by PCR. Panel B: Radish Plasmid DNA Plasmid Purification Kit Automated and manual sequencing,
RuBISCO cDNA. mRNA from
a single primary leaf (lane 1) or (27-9601-02) PCR, mutagenesis, restriction analysis
from 100 mg of primary leaves
(13 leaves) (lane 2) was purified FlexiPrep™ Kit (27-9281-01) Sequencing, restriction, labelling
using QuickPrep Micro mRNA
Purification Kit, then reverse- cDNA SizeSep™ 400 Spun Cloning
transcribed using First-Strand
cDNA Synthesis Kit and oligo Columns (27-5105-01)
(dT)12–18 as primer.The entire
reaction was amplified by PCR Sequencing AutoSeq 96 Dye Terminator Clean-up Kit Automated sequencing
using consensus RuBISCO primers. (27-5340-96, 27-5340-10)
Capacity/Scale Typical Yield Total Time Format
0.1 g tissue or Up to 6 µg/100 mg 15 minutes/batch Spin (batch);
1 x 107 cells tissue (90% purity) microfuge
0.5 g tissue or Up to 25 µg < 1 hour Spin column;
5x107 cells (90% purity) tabletop centrifuge
25 mg - 1 g cells or tissue 20 µg/1.25 mg total RNA (1 round) 30-45 minutes/batch Spin column; tabletop centrifuge
Up to 1 g tissue or 1 x 108 cells From mouse liver: 4.6 µg/mg tissue 1 hour Microfuge or standard centrifuge
Up to 4 g tissue/kit 3 mg total RNA/1 g calf liver Overnight Ultracentrifuge
10 g tissue/100 ml Varies Overnight Ultracentrifuge
25-50 µl > 80% recovery 4 minutes Spin column; microfuge
Up to 25 µl for removal of dye-labelled terminators > 80% recovery 4 minutes Spin column; microfuge

Up to 25 µl for removal of dye-labelled terminators > 80% recovery 4 minutes Spin column; microfuge
20 - 100 µl 60-95% 20 minutes 96-well plate, vacuum or centrifuge

PCR - up to 100 µl 60-90% PCR - 5 minutes Spin column;

Gel band - up to 300 mg Gel band - 15 minutes microfuge
10-100 µl 90% 4 minutes Spin column; microfuge
25-50 µl 60-80% 4 minutes Spin column; microfuge
25-50 PCR, Cloning 50-100 µl sequencing 50-70% 4 minutes Spin column; microfuge
0.75 g agarose per microfuge tube 50% < 30 minutes Spin (batch); microfuge
300 µl - 30 ml of whole blood 35 µg DNA/ml whole blood 1 hour Batch centrifugation
20 - 500 mg tissue 5 x 106 cultured cells 5 µg DNA/ml tissue (variable) 1 hour Batch centrifugation
10 µl – 1 ml whole blood 4 x 106 - 1 x 108 cells up to 15 µg 30 minutes for 18 preps GFX Spin Column, vacum or centrifuge
50 µl – 10 ml whole blood 1 x 106 - 1 x 107 cells 700 µg DNA/ 10 ml whole blood 30 minutes Microfuge or benchtop centrifuge

25 mg tissue sample 30 µm paraffin section 70 - 200 µg DNA/cm mouse tail 3 hours Microfuge or benchtop centrifuge
up to 0.1 g (small scale) up to 1.0 g (large scale) variable (10 – 60 µg/ 0.1 g prep) 1 – 1.5 hours Microfuge or benchtop centrifuge
Concentration 90% recovery < 15 minutes Gravity
1 mg/ml; 0.5-2.5 ml
100-150 µl of deprotection solution 85% 4 minutes Spin column; microfuge
1-3 ml cultures 3-6 µg/ml culture 30 minutes Spin columns; microfuge

1.5-500 ml cultures >2-4 µg/ml culture < 1 hour for minipreps Spin (batch); microfuge or tabletop centrifuge
cDNA made from up to Varies < 1 hour Spin column; tabletop centrifuge;
5 µg mRNA; 50-110 µl Sepharose CL-4B resin
15 - 20 µl / well > 65% recovery 15 minutes 96-well format, centrifuge
Advantages Notes
Speed; total RNA not required; For isolation of mRNA directly from cells and
functionally pure message tissue; not ideal for low-copy-number messages
Speed; individually packed; For isolation of mRNA directly
functionally pure message from cells and tissue Fig 2. Triplicate 10 µl,25 µl and 50 µl
samples of human whole blood were
Individually packed reagents mRNA isolation from total RNA processed using the direct method of the
GFX Genomic Blood DNA Purification
Speed; no hazardous organic solvents Not for use with bacteria or whole blood Kit. Purified genomic DNA was eluted
using 130 µl of TE buffer (pH 8.0).
One-tenth of each purified sample was
Good for RNase-rich sources Ideal for low-copy-number messages loaded onto an agarose gel.The volume
of blood that was processed is indicated
Strong chaotrope, inhibits ribonucleases Can be used to separate RNA and DNA above the corresponding lanes of the gel.
M = molecular weight marker.
Speed; simultaneous analytical and prep scale Alternative to TCA precipitations; sample elutes in 150 mM NaCl
Speed; pre-packed; high throughput Sample elutes in TE
when used with MicroPlex™ 24
Speed; versatility Sample elutes in water
Speed; high throughput; For DNA fragments
no hazardous organic solvents 100 bp - 9 kb AutoSeq, Flexiprep, Genomic Prep, GFX, MicroSpin,
Microplex, NAP, ProbeQuant, QuickPrep, Sephadex,
Speed; For DNA fragments Sephaglas and SizeSep are tradmarks of Amersham
also concentrates 100 bp - 10 kb Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham
plc. Pharmacia and Drop Design are trademarks of
Speed; versatility Pre-packed and pre-equilibrated Pharmacia Corporation. DyeDeoxy is a trademark
of Applied Biosystems Inc. Nucleon and PhytoPure
Speed; versatility Pre-packed and pre-equilibrated are trademarks of Tepnel Life Sciences plc.
*The Polymerase Chain Reaction (PCR) is covered
Speed; versatility Pre-packed and pre-equilibrated by patents owned by Roche Molecular Systems
and F Hoffmann-La Roche Ltd. A license to use
Speed; scalable Not for purifying PCR products from solution the PCR process for certain research and
development activities accompanies the purchase
No phenol/chloroform Scalable of certain reagents from licensed suppliers such as
Amersham Pharmacia Biotech Limited and affiliates
when used in conjunction with an authorized
No phenol/chloroform Scalable thermal cycler. All goods and services are sold
subject to the terms and conditions of sale of the
Speed, purity, high throughput with MicroPlex 24 No glass slurry, elution in low salt buffer company within the Amersham Pharmacia Biotech
group which supplies them. A copy of these terms
Purity, scalable, non-column means Novel protein binding resin and conditions is available on request.
high MW DNA, no Phenol © Amersham Pharmacia Biotech UK Limited 2000
– All rights reserved.
Purity, scalable, dedicated plant kit Novel polysaccharide binding resin Amersham Pharmacia Biotech UK Limited.
Amersham Place Little Chalfont Buckinghamshire
HP7 9NA England.
No phenol/chloroform Scalable Amersham Pharmacia Biotech AB
Accommodates large For buffer exchange/desalting Björkgatan 30 SE-751 84 Uppsala Sweden.
Amersham Pharmacia Biotech Inc.
volumes (0.5-2.5 ml) after oligo synthesis 800 Centennial Avenue PO Box 1327 Piscataway
NJ 08855 USA.
Speed; versatility; pre-packed microfuge For buffer exchange/desalting after oligo synthesis Amersham Pharmacia Biotech Europe GmbH
Munzinger Strasse 9 D-79111 Freiburg Germany.
Speed, ease of use Elution in low-salt buffer, MicroPlex
available for high throughput
Economy; scalable Elution in low-salt buffer, works with Cosmids also
Pre-packed; size selection Retains cDNAs < 400 bp; quality-control
of cDNAs tested for cDNA applications
Speed; economy; reduced disposal costs Template tolerance; 100 - 2500ng of M13
DNA or 80-2500 ng of plasmid DNA
MicroSpin Column Selection Guide
Our popular MicroSpin Columns, which are featured inside this selection guide, are very versatile
and can be used for many common molecular biology applications.

The following chart further discusses the applications of these microcentrifuge spin columns.
DNA Application Effect Resin Sample volume
PCR* Products Change of buffer following PCR Buffer exchange/desalting G-25 10-100 µl
Post-PCR clean-up for sequencing or other applications Removal of excess primers S-300 25-50 µl
excluding cloning S-400 50-100 µl
Post-PCR clean-up prior to cloning or clean-up between Removal of excess primers S-400 25-50 µl
sequential PCR reactions
Labelled DNA Clean-up of oligolabelling and end-labelling reactions Removal of free nucleotides Probe Quant G-50 25-50 µl
> 100 bases S-200 50-100 µl
Removal of DyeDeoxy™ terminators AutoSeq G-50 (water) 12-25 µl
MicroSpin G-50 (TE) 12-25 µl
Plasmid DNA Removal of NaOH from denatured plasmid DNA Buffer exchange/desalting
(RNase-treated) prior to sequencing G-25 10-50 µl
Removal of NaOH from denatured plasmid DNA Purification of plasmid DNA S-300 25-50 µl
(not RNase-treated) prior to sequencing from RNA/buffer exchange
Oligos Oligonucleotide purification following synthesis Buffer exchange G-25 100-150 µl
Removal of unincorporated nucleotides from end-labelled G-25 10-50 µl
oligonucleotides (>10 mers)
Unlabelled DNA Change of buffer between enzymatic reactions Buffer exchange/desalting G-25 10-100 µl
> 10 bases

Contact your local Amersham Pharmacia Biotech office:

Asia Pacific
Tel: +852 2811 8693
Fax: +852 2811 5251
Australasia
Tel: +61 2 9894 5152
Fax: +61 2 9899 7511
Austria
Tel: 01 57 606 1623
Fax: 01 57 606 1627
Belgium
Tel: 0800 73888
Fax: 03 272 16 37
Canada
Tel: 1 800 463 5800
Fax: 1800 567 1008
Central Europe
Tel: +43 1 982 3826
Fax: +43 1 985 8327
C.I.S. & N.I.S.
Tel: +7 (095) 232 0250
Fax: +7 (095) 230 6377
Denmark
Tel: 45 16 24 00
Fax: 45 16 24 24
Finland & Baltics
Tel: +358-(0)9-512 39 40
Fax: +358-(0)9-512 17 10
France
Tel: 01 6935 6700
Fax: 01 6941 9677
Germany
Tel : +49 0761 49 03 403
Fax: +49 0761 49 03 246
Italy
Tel: 02 27322 1
Fax: 02 27302 212
Japan
Tel: 81 3 5331 9336
Fax: 81 3 5331 9370
Latin America
Tel: +55 11 3667 5700
Fax: +55 11 3667 8799
Middle East and Africa
Tel: +30 (1) 96 00 687
Fax: +30 (1) 96 00 693
Netherlands
Tel: 0165 580 410
Fax: 0165 580 401
Norway
Tel: 23 18 58 00
Fax: 23 18 68 00
Portugal
Tel: 21 417 70 35
Fax: 21 417 31 84
South East Asia
Tel: 60 3 724 2080
Fax: 60 3 724 2090
South East Europe
Tel: +43 (1) 982 3826
Fax: +43 (1) 985 8327
Spain
Tel: 935 944 950
Fax: 935 944 955
Sweden
Tel: 018 612 1900
Fax: 018 612 1910
Switzerland
Tel: 01 802 81 50
Fax: 01 802 81 51
UK
Tel: 0800 616 928
Fax: 0800 616 927
USA
Tel: +1 800 526 3593
Fax: +1 877 295 8102

Or visit us at: www.apbiotech.com