MPL103T

Professional Course Examination, November 2018

( CBCS )
( 1st Semester )

MASTER OF PHARMACY

( Pharmacological and Toxicological Screening Methods—I )

Full Marks : 75

Time : 3 hours

( PART : A—OBJECTIVE )
( Marks : 25 )

The figures in the margin indicate full marks for the questions

SECTION—A
( Marks : 10 )

Choose and write the correct answer : 1×10=10

1. The preanaesthetic medication used for avoiding cardiopulmonary

problems and decrease saliva production in laboratory animal is

(a) xylazine

(b) atropine

(c) diazepam

(d) meprobamate

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 2. In a multiple withdrawal of blood sample, blood volume should not exceed
more than _____ of total blood in a day.

(a) 1%

(b) 2%

(c) 4%

(d) 8%

3. The agonist for GABA A receptor is

(a) bicuculline

(b) picrotoxin

(c) muscimol

(d) baclofen

4. Reserpine can be used to produce animal model of

(a) diabetes

(b) mania

(c) hypertension

(d) depression

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 5. Which of the following is not genetically diabetic animal?

(a) BB Rat

(b) WBN/Kob Rat

(c) Goto-Kakizaki Rat

(d) FA-TA Rat

6. Which of the following methods is not a model for analgesic drug

evaluation?

(a) Hot plate method

(b) Acetic acid-induced method

(c) Pyloric ligation method

(d) Tail flick method

7. Experimental animal model of atherosclerosis can be induced by high _____

diet.

(a) cholesterol

(b) triglyceride

(c) protein

(d) lipoprotein

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 8. The chemical which is not used for induction of ulceration for evaluation of
anti-ulcer activity is

(a) ethanol

(b) furosemide

(c) aspirin

(d) indomethacin

9. The competitive immunoassay can be used

(a) to detect a very small amount of antigen

(b) to detect antibodies associated with allergens

(c) Both (a) and (b)

(d) commonly to detect trace amount of drug

10. What is the ELISA test intended to measure?

(a) Antibody to HIV

(b) Antigen to HIV

(c) Presence of free circulating virus in a patient

(d) antibody directed against HLA molecules

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 SECTION—B
( Marks : 15 )

Answer any five of the following questions : 3×5=15

1. Write a note on experimental induction of atherosclerosis.

2. Explain the procedure of strychnine-induced convulsion in mice.

3. Discuss the evaluation of anti-ulcer activity by different stress models in

rats.

4. Write a note on evaluation of analgesic activity in animal models.

5. Discuss the role of CPCSEA in experimentation on animals.

6. What is HeLa cell? Write the use of HeLa cell in research.

7. Discuss the method of ethanol-induced ulceration in rat.

( PART : B—DESCRIPTIVE )
( Marks : 50 )

The questions are of equal value

Answer any five questions

1. Describe in detail the principle and method of MTT assay.

2. Explain the different techniques used for creating transgenic animals.

3. Briefly discuss with examples the limitation of extrapolation of preclinical data

to human.

4. What is immunoassay? Discuss the therapeutic applications of immunoassay.

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 5. Write the basic principle of in vivo evaluation of antipsychotic agents. Describe
one of the methods in detail.

6. Explain the principle and procedure of Lipschütz test of anti-diuretic activity.

7. Describe the procedure of streptozocin-induced diabetes.

8. Explain in detail the principle and procedure of carrageenan-induced paw

oedema in rat.

HHH

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