UREA KIT (GLDH Kinetic method) Far he detention of Ure in serum or plasma (Forni Diagnostic Use Cn) ‘summary Ursa is te ené product of pris mtaboisn. hs synthessed ine er fom he anon producod by he Catadolsm of nino sods. is ranspored bythe ood 0 the Kiénoys tom where its excl. nased lve are ‘ound In tena! dseases, wriary obseuctons, shock gesve hear fur and bums. Deceased levels are ‘odin Ive: Talureand pregnancy Principe Ureasehytlyes urea to anmoraandCO, Thesis fomoc tor cambiras wth cKelogluarata and NADH io ‘om Glutamate and NAD Tate of oxiation of NADH > NAD Is measured a a deteate ho abeobanca na fee time wich proporiona to the rea coneeiaten Inthe sani. nora MM aecece ‘ow the Working Reagent to stand for 30 min. before Sample material Serun, asa, Ue Divo wine +49 wih eed water boforetha assay resutsx50) Urals raped abe stable intheserumioeScayswhen sioredat2-8C. Procedure Wavelength ifiter 40am Temperate sreiwerase Uahioath tom Substrate Start Assay 0 a dean dry test ie label Sardard (8) or “adaon om Sequence sronmerse nase Reageat CT Osi ‘oa Stanear/SeumDintd urna [007 mi Incubate the asaytperaure for rina ane od Slr Reagent (12) oam 2 2a 04 OL gtetes 2040 Nomalrefrence valves Sorum/ Plasma 14 40mg Upiezaai Is reoammondes tht each labasion etabieh sown nal rangareprseningitspatenl opJatn. Contents Lt Ene Reagent Lz: tater Reagent $5: Urea Sandars (0 mg) 5 ml 2x78m 2x 150 m1 0m 2x60mi 2x 120m 18m 2x 18m 2x30 Smi Sm ‘Storage stabity Contes are lable at 24°C ne expy mentioned on she abel. Reagent Preparation Reagentsareready use Working reagent: Forsanplesartassaysasiglreagert & ragured. Pou the cntonts of 1 bate of L2 (Staner Reagenjinio tote oft (Enayme Reagent) Tiswakng reagent istbleforalleas10days when storedat 2. Aerator febity 25 mach of working reagent may Bemade and when dsredby ming together & parts of 1 (Enzyre Reagent and 1 parol L2 (Starter Reagent ‘Marne OB miofLt and.2nt of 2 may aoe used instead of 1 mi ofthe werong reagent recy dung the assy, x wall and0aé ota absotance foto Standard fand Test ator exacly 30 seconds, Read another oerbanced ofthe Standard and Tet exact 0 seconde late Caluat he change in absorbance Aor both the Standardana Tes. Sample Stat Assay: Pit rio a clan dry lest be labels Sardard (8) or Tost) ‘aaltion ul Sequence reverse Workngreager cr Bingioasayenporatureandad UreeStenderd'SerumiDistedurne| 001ml x wo and ea thrill absorbance A or te Standard tard Test aor exacly 30 scones, Read aroher sortancoA, lth Standard and Tot exact 0 saconds late Calculate the change absorbance AA or oth he Slandardand Test. ForStandad AAS: AS + AS Forres, aat AT. AT Caleulatons oat Usa in gi . x a assLinearity Ths procedures osarupo 250 al. vais exceed ths it alto to serum wh noma saline (NaCl 0.) sd epeathe assy Cals he valu us he proper lutte, Pama should not be colcted wih Flutie or Hapa Salsas covaninaionyanmoriacrammonumsas ead toaranecue resus References Fancel 1X, Seo J, (1860) J. Chin Pao. 19-186 Choy. (1962) Cin. Chem 8: 130 System Parameters Reaction Fite Te Knete Wavelongth = 340m Zero Setting: Distles Water Incub. Temp, : 30°07 37°C Incub. Time: — Delay Time 90sec. ReadTime : b0see, Novofread, 2 Intent see, Sample Vol. + G0" mi Reagent Vol. : 1.00mi Standard 4D mp Factor = React Slope: Decreasing Linearity”: 280 mg Unis nos al Systems Gana Dr Aioia Oo Rego Bop, Samboin Complex 20, GOA. 463202, INDIA