NAME.

PRANAV ROY
ROLL NO. 36

BIOCHEMISTRY MCQ ASSIGNMENT.

1. A male infant of 6 month was brought to the casualty following an
attack of moderate generalized convulsions about 2 hours back. He was
born after a normal delivery and had taken feeds normally. However on
examination the child was found to have delayed milestones. He was not
holding neck and could not sit without support even at 6 months of age.
The mother also gave a history of mousy odour in child's urine. There was
no history of abnormal child births with congenital defects in the family.
The paediatrician suspected a metabolic disorder and ordered the
screening of blood and urine for various amino acids. Please suggest the
best technique available for the screening
Ans. Tandem mass spectroscopy

2. An obese person came to the hospital with complaints of polyuria, thirst,

weakness and increased appetite. on investigation random blood sugar was
found to be 170 mg/dl. Doctor has asked HbA1C for further investigation.
Which method is best for HbA1C measurement?
Ans. HPLC

3. Enzyme increase the rate of reactions by

Ans. decreasing the energy of activation
4. A polysaccharide made up of D-galactose and 3,6-anhydro-L-
galactopyranose units which are used as matrix for electrophoresis, is:
Ans. Agarose

5. Beer-Lambert Law gives a linear correlation with positive gradient

between:
Ans. wavelength and absorbance.

6. The Km of enzyme is
Ans. The substrate concentration that gives half maximal velocity

7. What is the color coding of the bag used in hospitals to dispose of

human anatomical wastes such as body parts:
Ans. Red

8. A 9 years old child brought to the hospital with complains of black

discoloration of ear and nose. His urine turns black on standing. He also
had history of joint pain due to repeated arthritis
Q1. Please write the diagnosis
Alkaptonuria
Q2. which is the pathway involved ?
Homogentisic acid ----------> Benzoquinone acetate
Q3. which is the enzyme deficit in this disease
Homogentisic acid oxidase
Q4. Name at least one test that can be done to confirm the disease
Benedict's test

9. A patient was diagnosed as having alkaptonuria.

a. Outline the biochemical pathway and point out metabolic defect which
leads to this condition.
Pathway is catabolism of tyrosine and defect is of enzyme
Homogentisic acid oxidase
b. State the changes in urine, on standing, in such patients. Why?
Urine becomes black on standing due to formation of
Benzoquinone acetate
c. What is ochronosis?
Alkaptone bodies deposited in intervertebral discs, joints at the
age of 3rd and 4th decade of life.
d. What is the treatment?
Reduce phenylalanine in diet, reduce protein diet

10. When requesting laboratory tests for possible multiple myeloma, which
of the following is the single most useful test?
Ans. Serum protein electrophoresis
11. The fluorescent dye such Ethidium bromide is used for visualizing DNA.
How do ethidium bromide binds to DNA?
Ans. Stacked between histone molecules

12. Statins are competitive inhibitors of HMG CoA reductase. Which of the
following statements about competitive inhibitors is correct?
Ans. Competitive inhibitors are examples of irreversible inhibitors.

13. At isoelectric pH, the amino acids and protein show:

Ans. Maximum Precipitation.

14. A 50-year-old man returned from Wuhan (China). He had acute

respiratory distress syndrome (ARDS) and also met the following criteria:
severe pneumonia with rapid progression and continuously high viral load
despite antiviral treatment; Paoz/Fioz <300. Please mention the best
available techniques for diagnosis of disease
Ans. Real Time PCR

15.