1-The enzymes which are used to cut out the gene of interest are known as:

(A) DNA ligase (B) DNA Polymerase (C) RNA Polymerase (D) Restriction
endonuclease
2-Eco. R is commonly used:
(A) Gene (B) Restriction (C) Bacteriophage (D) Bacteria
enzyme
3-The full set of genes of an individual is called:
(A) Genome (B) Genotype (C) Karyotype (D) Dominance
4-PCR takes its name from_________________ the enzyme that carries out DNA replication in cell.
(A) DNA polymerase (B) DNA polymerase (C) DNA ligase (D) Restriction enzyme
II
5-Transgenic bacteria are produced in large vats called:
(A) Transducer (B) Bioreactor (C) Biomultiplier (D) Culture media
6-Meristem is:
(A) Virus free (B) Bacteria free (C) Fungi free (D) Pathogen free
7-The enzyme luciferase is produced by an insect commonly known as _________________.
(A) House fly (B) Butter fly (C) Caddis fly (D) Fire fly
8-Antibody used for the treatment of genital herpes is obtained from:
(A) Corn (B) Soya-bean (C) Rice (D) Wheat
9-In ocean of tropical regions, the temperature of surface water is about:
(A) 5C (B) 10 C (C) -5 C (D) 25 C
10-Population of Pakistan in the year 1947 was ______ million.
(A) 32.5 (B) 50 (C) 150 (D) 180
11-Study of human populations and the things that affect them is called _________.
(A) Human biology (B) Social biology (C) Demography (D) Teratology
12-The increase of environmental temperature due to high amount of CO2 is known as ___________.
(A) Global warming (B) Acid rains (C) Ozone depletion (D) Stone cancer
13-The decline of thickness of ozone layer is caused by increasing level:
(A) Chloroflourocarbons (B) Nitrogen (C) Hydrogen (D) Carbon dioxide
14-which disease is due to mental illness:
(A) Scurvy (B) Goiter (C) Beriberi (D) Alzheimer
15-The percentage of land under cultivation is_______%.
(A) 30 (B) 20 (C) 11 (D) 5
16-Ozone depletion is commonly caused by;
(A) CFCs (B) CO2 (C) Smoke (D) Smog
Q.No.1: Choose the best choice for the statements given below. 1 x 16 = 16
Q.No.2: Give answer of all short questions. 2 x 8 = 16
i-What are restriction endonucleases? v- What is gene therapy?
ii- What is probe? Give its role. vi-What is gene pharming? Explain its importance.
iii- Write two uses of PCR. vii-Define wild life. Give its important role.
iv- Define bioreactors. viii-Give some ways to conserve energy on earth.
Q.No.3:Write a note on following: 4x2=8
a- Write a note on green house effect.
b- Describe the causes and effects of acid rain.