Judul : The Association of Methylenetetrahydrofolate Reductase Genotypes with the Risk of

Childhood Leukemia in Taiwan

LB: Leukimia lymphoblastic akut adalah jenis kanker pediatric yang paling umum, penyebabnya
adalah adanya interaksi antara faktor genetik dan lingkungan.
Tujuan : Untuk mengevaluasi efek efek dari genotypic polymorphisms pada MTHFR di anak-
anak yang berisiko Leukimia lymphoblastic akut di Taiwan, dua tipe polymorphic genotypes
yang terkenal dari MTHFR, C677T (rs1801133) dan A1298C (rs1801131) dianalisis untuk
memeriksa sejauh mana asosiasinya dengan susceptibility anak-anak leukemia lymphoblastic
akut dan untuk mendiskusikan kontribusi dari MTHFR genotypic terhadap susceptibility anak-
anak penderita leukemia lymphoblastic akut diantara populasi yang berbeda.
Metode :
Desain studi: case control
Sampel: All subjects voluntarily participated, completed a questionnaire with the help of parents
or guardians and provided peripheral blood samples. The questionnaires recorded their disease
history, diet and sleep lifestyles and the disease history, diet and behavioral lifestyle, social-
economic status of the parents. An equal number of age-matched non-cancer healthy volunteers
were selected for use as a control group following initial random sampling from the Health
Examination Cohort established from Apr 2005 to Jan 2010 as previously published [23].
Metode pemeriksaan: metodologi PCR-RFLP
266 pasien anak leukemia lymphoblastic akut dan sejumlah (jumlah yang equal) orang yang
tidak menderita kanker sebagai grup control yang direkrut dirubah secara genotyped
menggunakan metodologi PCR-RFLP. Genotype MTHFR C677T, tapi bukan A1298C secara
berbeda didistribusikan diantara anak-anak penderita leukemia lymphoblastic akut dan grup
control.
Hasil: Genotype CT and TT dari MTHFR C677T secara signifikan lebih sering ditemukan di
grup control daripada anak-anak dengan leukemia lymphoblastic akut (odds ratios=0.60 and
0.48, 95% confidence intervals=0.42–0.87 and 0.24–0.97, respectively). As for gender, the boys
carrying the MTHFR C677T CT or TT genotype conferred a lower odds ratio of 0.51 (95%
confidence interval=0.32–0.81, P=0.0113) for childhood ALL. As for age, those equal to or
greater than 3.5 years of age at onset of disease carrying the MTHFR C677T CT or TT genotype
were of lower risk (odds ratio= 0.43 and 95% confidence interval=0.26–0.71, P=0.0016).
Kesimpulan : Our results indicated that the MTHFR C677T T allele was a protective biomarker
for childhood ALL in Taiwan, and the association was more significant in male patients and in
patients 3.5 years of age or older at onset of disease.