1.

List the products of light dependent reaction

NADPH,ATP ,Oxygen

2. Define photophosphorylation
Process of adding phosphate to ADP to form ATP by using the energy from sunlight via electron
transport chain and chemiosmosis .

3. Compare Cyclic and Noncyclic photophosphorylation Now, answer these questions:

Cyclic photophosphorylation Noncyclic photophosphorylation

Involve cyclic electron flow Involve non-cyclic electron flow

Only produce ATP
Involves PS I (P700) only
First electron donor is PS I
Last electron acceptor is PS I
No oxygen release
Produce ATP, oxygen and NADPH + H+
Involves PS I (P700) and PS II (P680)
First electron donor is water
Last electron acceptor is NADP+
Oxygen is released

4. Compare Photosystem I and Photosystem II

Photosystem I
The photocenter is P700
Pigments absorb longer wavelengths of light
(more than 680 nm)
Involved in both cyclic and non- cyclic
photophosphorylation
No photolysis of water occurs
Main function is NADPH synthesis
Photosystem II
The photocenter is P680
Pigments absorb shorter wavelengths of light
(less than 680 nm)
Only involved in cyclic photophosphorylation
Photolysis of water occurs
Main function is ATP synthesis and hydrolysis
of water

5. Definition of chemiosmosis.

Chemiosmosis is the process that use energy stored in the form of proton (H+) gradient across
the inner mitochondrial membrane to produce ATP from ADP and Pi. Proton (H+) across
membrane is known as proton motive force.

6. Why is it called oxidative phosphorylation in mitochondria?

It is because hydrogen ions in the matrix space can only pass through the inner mitochondrial
membrane through a membrane protein  called ATP synthase. As protons move through ATP
synthase, ADP is turned into ATP. The production of ATP using the process of chemiosmosis in
mitochondria is called oxidative phosphorylation