Name: ______________________________ Student No.

: ____________
Year and Section: _____________ Date: __________

Laboratory Exercise
FECAL STRUCTURES

Illustrate/cut-down pictures of the all the fecal structures demonstrated microscopically.

Write the distinct characteristics of each and indicate to which parasite’s diagnostic stage it is
confused with.

Fecal Structure Characteristics Confused as

Vegetable cell (400x)

Vegetable Spiral (400x)

Vegetable Fiber/hair (400x)

Miscle Fiber (400x)

Air Bubbles (400x)

Fat Globules (400x)

Starch Granules (400x)

Yeast cell (400x)

Charcot Leyden Crystal

(400x)
 Laboratory Exercise
DEMONSTRATION OF DIAGNOSTIC TECHNIQUES IN STOOL PROCESSING
Direct Fecal Smear (DFS)

1. Preferred size for DFS?

2. Wet mount is most useful method looking for__________ which can be observed by their
characteristic movement and appearance.
3. The iodine will stain the organisms a dark orange brown color. This is most useful in
diagnosing what parasite?
4. Iodine stain kills what parasite?
5. When does BMB (buffered methylene Blue) wet mount used?

Laboratory Exercise
Test Yourself
SCOTCH TAPE SWAB

1. Other name for scotch tape swab?

2. This is the best method used for diagnosing what parasite?
3. Preferred time of collection?
4. Describe characteristic of the ova that could possibly see in this method.
5. What is the mode of transmission for this parasite?
 Laboratory Exercise
Test Yourself
Concentration Technique

1. What is the principle of flotation technique?

2. What are the contents of sheather’s sugar solution?
3. What parasites can be recovered using sheater’s sugar flotation?
4. Through Formalin Ether Concentration Technique (FECT). Label the layer of
Centrifuged sample. Four layers will form after centrifugation.

5. What are the other solutions that could be used for floatation and sedimentation
technique?
 Laboratory Exercise
Test Yourself
Kato-Katz Technique

1. Solution used for kato-thick smear

2. The difference of direct fecal smear from kato-thick and katz smear?
3. Other name used for Kato method?
4. Glycerine is used in this solution as?
5. Viewing and examination of cellophane-covered thick smear under______ and switch to
a ______________ for detailed microscopic examination.

Laboratory Exercise
PREPARATION OF BLOOD FILMS

1. Anticoagulant used in Thick and Thin smear preparation

2. What is the importance of dehemoglobinizing the thick blood smear by dipping it in
tap water?
3. Usual site for needle prick for Thick and Thin smear preparation
4. What is the purpose of Thick smear?
5. What is the purpose of thin smear?