3/12/2020 COS-7 Cell Line 87021302, from African green monkey kidney, SV40 transformed, Convention on the International

International Trade in Endangered …

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COS-7 Cell Line

87021302, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

SKU-Pack Size Availability Safety Price (EUR)

87021302-CDNA-20UL Estimated to ship on 25.03.20 SDS 346.00

87021302-DNA-5UG Estimated to ship on 25.03.20 SDS 358.00

87021302-RNA-5UG Estimated to ship on 25.03.20 SDS 417.00

87021302-1VL Only 1 left in stock (more on the way) - FROM SDS 501.00

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Properties
Related Categories A-C, CITES, Cell Culture, ECACC Cell Lines

biological source African green monkey kidney

growth mode Adherent

karyotype Not specified

morphology Fibroblast

products Not specified

receptors Not specified

application(s) cell culture | mammalian: suitable

shipped in dry ice

storage temp. −196°C

Show Fewer Properties

Description
Cell Line Origin
Monkey African green kidney, SV40 transformed

Cell Line Description

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be
handled at laboratory containment level 2.

Application
COS-7 Cell Line has been used to:
• test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
• study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and
regulatory domains
• study the distribution of peroxisomes

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3/12/2020 COS-7 Cell Line 87021302, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered …

Virus replication studies: SV40

Culture Medium
DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..

Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the
depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the
potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety Information Documents

RIDADR NONH for all modes of transport Certificate of Analysis (COA)

WGK Germany 3 Enter Lot Number

How to enter a Lot Number

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Protocols & Articles

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3/12/2020 COS-7 Cell Line 87021302, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered …

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