3/12/2020 CHO Cell Line from Chinese hamster ovary from hamster ovary, 85050302 | Sigma-Aldrich

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CHO Cell Line from Chinese hamster ovary

from hamster ovary, 85050302
Synonym: Chinese hamster ovary cell line

SKU-Pack Size

85050302-DNA-5UG

85050302-RNA-5UG

85050302-1VL

 
CHO-K1 Cell Line from CHO Protein-Free Cell CHO-K1.Cl-0204 CHO/dhFr-AC-free cell line CHO/dhFr-
hamster Line from Chinese ... 15042903, from hamster ovary 05011002 94060607
from hamster ovary, 85051005 00102307

Properties
Related Categories A-C, Cell Culture, ECACC Cell Lines More...

biological source hamster ovary

availability not available in Japan

growth mode Adherent

karyotype Hypodiploid, modal no. 20

morphology Epithelial

products Not specified

receptors Not specified

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Description
Cell Line Origin
Hamster Chinese ovary

Cell Line Description

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Application
CHO Cell Line from Chinese hamster ovary has been used:
• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

Culture Medium
Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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3/12/2020 CHO Cell Line from Chinese hamster ovary from hamster ovary, 85050302 | Sigma-Aldrich

Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the
depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the
potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety Information Documents

RIDADR NONH for all modes of transport Certificate of Analysis (COA)

WGK Germany 3 Enter Lot Number

How to enter a Lot Number

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