Professional Documents
Culture Documents
of DNA
Experiment Goals
• Digestion of DNA by restriction enzyme
5’ GAATTC 3’
3’ CTTAAG 5’
HaeIII
Restriction Enzyme Uses
1. Recombinant DNA technology
*Discovery of enzymes that cut and paste DNA make
genetic engineering possible.
Digestion of DNA
• Mix the following components in a clean microtube.
• Mix gently and spin down for a few seconds.
• Incubate at 37°C for 16 hours.
Analysis of DNA digestion
• Analyze products on 2% agarose gel containing
ethidium bromide.
Undigested DNA is represented by a sharp band near the wells of the gel, while
smearing indicates digested DNA sample.
Exercise
Q1 Restriction enzyme AluI which recognize
sequence
5’ AGCT 3'
3' TCGA 5‘