Lab Session #9: Summary Lab Report

Title of Lab:

Your Name:

Data and Results:

Carbohydrate Tests

Benedict’s Test (for reducing sugars)

Observation Conclusion
(reducing, nonreducing or inconclusive)
Water

Glucose
Fructose
Sucrose
Lactose
Starch
Unknown

Seliwanoff’s Test (for ketoses and aldoses)

Observation Conclusion
(ketose, aldose, or inconclusive)
Water

Glucose
Fructose
Sucrose
Lactose
Starch
Unknown

Fermentation Test
Observation Conclusion
1
 (Fermentation: yes or no)
Water

Glucose
Fructose
Sucrose
Lactose
Starch
Unknown

Iodine Test (for Polysaccharides)

Observation Conclusion
(amylose: yes or no)
Water

Glucose
Fructose
Sucrose
Lactose
Starch
Unknown

Hydrolysis of Disaccharides and Polysaccharides

Conclusion
Iodine Test Observation Benedict’s Test Observation
(Hydrolysis: yes or no)
Sucrose + H2O
Sucrose + HCl
Starch + H2O
Starch + HCl

2
Carbohydrates in Food

Benedict’s Test Seliwanoff’s Test Iodine Test Carbohydrates present

Powdered Sugar
Cheetos
Cornflakes
Brown Sugar
Oats
Unknown

Unknown #2

Benedict’s Test Fermentation Seliwanoff’s Test Iodine Test

Observation
Type of Carbohydrates

Based on the physical and chemical properties you observed in your unknown and each of the carbohydrates,
identify your unknown and explain fully how you came to your conclusion.

You believe your unknown is . .

Explain fully based on the results of at least 3 test/observations:

POST-LAB QUESTIONS:
3
 1. In the hydrolysis experiment, in which test tubes did hydrolysis occur? Explain how you know hydrolysis
occurred based on the observations seen.

2. Imagine you have 3 vials containing 3 different clear liquids. You are told that each vial contains one of the
following carbohydrate solutions: Fructose, Sucrose, or Starch. What tests could you carry out to
determine the composition of each vial? Be sure to include the name of the test as well as the expected
observations.

4
Summary:
1. What was the purpose(s) of this experiment?

2. Evaluate the data: What were the major results? Were the results bad, mediocre, good, or
excellent? Explain.

3. Error Analysis: Give an example of two theoretical errors that could have taken place that would
have given bad results. I know that you did not perform the experiments, but based on what you’ve
read, what experimental errors could have happened that would have provided less than excellent
results? (Do not say “human error” or “calculation error” as an explanation. Look back through
the experimental procedures and suggest where errors could have been made.)

4. Reflection: Explain at least three different things you learned in this lab session.