AIM: To determine the amount of vanilline in sample of vanilla essence by condutometry.

REQUIREMENTS: Vanilla essence, 0.05 N KHP soln., 0.05 N NaOH, conductometer, burette,
pipette etc.

REAGENT: Sample of vanilla essence, 0.05 N KHP, 0.05 NaOH soln.

Observation:
[A] Std. of NaOH
Burette : 0.05 N NaOH soln
Flask : 10 ml 0.05 N KHP soln
Indicator : Phenolphthalein
End point : colorless to pink.

Observation table:

Initial Burette Final Burette Volume of KOH Mean titre

Sr. No.
reading ‘ml’ reading ‘ml’ used ‘ml’ ‘ml’

1.
2. ‘X’ ml
3.

Normality of NaOH:
N1V1= N2V2
N1 = ‘X’ * 10
B. R.
= ______ N NaOH (A)

[A] Actual Experiment :

Burette : ___ (A) N NaOH soln.
Flask : 25 ml diluted soln. of vanilla + 25 ml D.W.
Conductivity of distill water _____ S

Observation table:
Sr. No. Conductance of Correct conductance = Specific conductance
solution conductance of solution –  = Correct conductance * cell
(mS) conduction of water (ms) constant (S.cm-1)
1.
2.
3.
4.
5.
6.
7.
 8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.

Procedure:
1. First std. 0.05 N NaOH soln. using 0.05 N KHP using phenolphthalein indicator.
2. Now take 5 ml of vanilla essence into a 100 ml volumetric flask and make it up to the
mark using double distilled water. take 25 ml of this diluted solution of vanilla essence
in a small beaker add 25 ml water in it and dip conductivity cell into it and measure
conductivity.
3. Now add 1 ml of 0.05 N NaOH soln., shake well and note conductivity. Repeat every
time up to about 20 ml of 0.05 N NaOH.

GRAPH:  vol. of NaOH.

CALCULATION:
From Graph
Vol. of NaOH for vanilla = 10.0 ml
1000 ml 1 N NaOH = 152.0 g vanilla
‘Y’ ml N NaOH = 152 *A *10.0
1000
= ________ g (B)

25 ml diluted soln. contains ____ (B) g of vanilline

Therefore, 100 ml solution contains B * 4 = ______ (C) g of vanilline

% of vanilline in vanilla essence = C * 100

5
= ______ %
RESULT:
1. Percentage of vanilla in essence = _____ %.