Real-Time PCR

High-Throughput Real-Time PCR Solutions

Efficiency and Flexibility at Every Step of Real-Time PCR
 ■■ Genetic Disease Research
■■ Oncology Research
■■ Stem Cell Research
■■ Pharmacogenomics
■■ Pathogen Detection
■■ Agricultural Biotechnology
■■ Lead Screening
■■ Lead Optimization
■■ Target Discovery
■■ Target Validation
■■ Biomarker Studies
■■ CRISPR Validation

HIGH THROUGHPUT. FLEXIBILITY. RELIABILITY.

Bio-Rad’s high-throughput real-time PCR products leverage more than
30 years of expertise to support your application and workflow.

2 | BIO-RAD LABORATORIES, INC.

HIGH THROUGHPUT

MAXIMIZED THROUGHPUT. REDUCED MANUAL INTERVENTION.

Traditional real-time PCR workflows involve multiple unreliable and labor-intensive steps
that limit throughput, process efficiency, and scalability. We provide a suite of products that
simplifies and accelerates real-time PCR to permit true high-throughput gene expression
profiling and quantification.

BIO-RAD WORKFLOW TRADITIONAL WORKFLOW

5 STEPS VS. 10 STEPS

GENE TARGET SELECTION GENE TARGET SELECTION

ASSAY DESIGN

PrimePCRPrimePCR
Gene Expression
Gene Expression ASSAY VALIDATION
Assays Assays

PLATE DESIGN

SAMPLE PREPARATION SAMPLE PREPARATION

REACTION SETUP REACTION SETUP

PrimePCRPrimePCR PROTOCOL SELECTION

Run FilesRun Files

DATA ACQUISITION DATA ACQUISITION

CFX Maestro SOFTWARE DATA ANALYSIS

ANALYSIS REPORT

RESULTS

BIO-RAD LABORATORIES, INC. | 3

HIGH THROUGHPUT

Benchspace Layout
Bio-Rad Competitor

1
CFX Real-Time
PCR Detection
30 in. (76 cm)

System

vs.
2 1 2
CFX Real-Time CFX Competitor Competitor Competitor Competitor
PCR Detection Automation Real-Time PCR Automation Real-Time PCR Automation
System System II System Robot System Robot

43 in. (109 cm) 97 in. (246 cm)

Twice the throughput. Half the footprint.

The CFX Automation System II can operate two CFX Real-Time PCR Detection
Systems simultaneously, doubling throughput and saving valuable benchspace,
compared to other automation systems.

Flexible System Configurations

CFX Real-Time PCR Detection Systems and Interchangeable Module
CFX Maestro Software provide:
■■  aboratory information management system
L
(LIMS) and automation compatibility
■■  arcode-specific cycling protocols that can
B
be assigned to each plate, with or without
LIMS integration
■■  FX Maestro Software, Security Edition to
C
support U.S. FDA 21 CFR Part 11 regulations
■■ Interchangeable 96- and 384-well reaction
modules for real-time PCR
■■ Customized run setup and data analysis Quantitative PCR
■■ Multi-instrument connectivity Needs Standard Throughput Large Reaction Volume High Throughput
■■ 5-target multiplexing Reaction Module CFX96 CFX96 Deep Well CFX384

■■  emote notification of run completion with

R
attached data file

From Plates to Analyzed Data in Three Clicks

PrimePCR
run files

1 Download run files 2 L oad run files into 3 C

 omplete statistical analysis
automation software with CFX Maestro Software

4 | BIO-RAD LABORATORIES, INC.

FLEXIBLE AND CUSTOMIZABLE

FLEXIBLE SYSTEMS. CUSTOMIZABLE ASSAYS.

Real-time PCR reagent, instrument, and software requirements are as diverse as
the products they help innovate. To address this, we have built flexibility, customizability,
and time-saving solutions into our entire product line to maximize your throughput
and workflow.

Supermixes for Every Sample Customizable Real-Time PCR Assays and Arrays
Our SsoAdvanced Supermixes utilize patented* Sso7d fusion polymerase Bio-Rad’s PrimePCR Gene Expression Assays are
technology, which increases processivity and inhibitor tolerance to ensure superior expertly designed, and panels are curated to include
performance even on challenging samples. Supermixes are available as custom only biologically relevant genes. PrimePCR Assays
formulations and in custom sizes. Products include: are available as:
■■  soAdvanced Universal SYBR® Green and Probes Supermixes
S ■■  YBR® Green– and probe-based
S
■■ SsoAdvanced Universal Inhibitor-Tolerant SYBR® Green Supermix individual assays
■■ SsoAdvanced PreAmp Supermix ■■  ustom predesigned disease and
C
■■ Reliance One-Step Multiplex Supermix pathway panels
■■ SingleShot Cell Lysis RT-qPCR Kits ■■  et-lab validated and next-generation
W
sequencing verified assays for the human,
rat, and mouse genomes
■■  xpertly in silico–designed assays
E
or a range of other model organisms

Reliable real-time PCR data from

even the most challenging samples.
SsoAdvanced Universal Inhibitor-Tolerant
SYBR® Green Supermix is a high-
performance real-time PCR supermix that
allows generation of superior data using
crude lysates from plants, seeds, cells,
bacteria, and formalin-fixed paraffin-
embedded samples.

Comprehensive Portfolio of Consumables

Our line of robotics-compatible real-time PCR consumables was designed
specifically with automation in mind. Products include:
■■ Temperature-stable supermixes
■■ Customizable, warp-free Hard-Shell PCR Plates and accessories
■■ One-step reverse transcription quantitative PCR (RT-qPCR) kits
■■ Kits for RT-qPCR directly from lysate

From tissue culture to real-time PCR without time-

consuming RNA purification steps. Prepare real-time
PCR–compatible cell lysates in a single 10 min step using
SingleShot Cell Lysis RT-qPCR Kits.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

BIO-RAD LABORATORIES, INC. | 5
REGULATORY TOOLS AND SERVICES

INSTRUMENT VALIDATION AND QUALIFICATION

Bio-Rad provides a comprehensive suite of products and 4 Thermal Validation Services
services that ensures your instruments are cared for and 4 IQ/OQ
perform to our standard of quality, run after run. 4 Qualification Plates
4 Expert Care Services

On-Site Thermal Validation Services CFX Qualification Plate

To ensure optimal performance of our CFX The CFX Qualification Plate provides a reliable and fast way to qualify
Real-Time PCR Detection Systems, a Bio-Rad any CFX Real-Time PCR Detection System. These sealed and frozen
trained and certified service engineer will: instrument qualification plates contain predispensed reagents and offer:
■■  rovide a thermal validation report clearly
P ■■ 1-click pass/fail results
documenting instrument performance ■■ Easy integration into quality control procedures
■■  ssess PCR block accuracy, thermal
A
■■ Convenient troubleshooting
uniformity, and instrument ramp rate
■■  onfirm thermal block settling times and
C
overshoot are within specifications

On-Site Installation Qualification (IQ) and

Operational Qualification (OQ) Services
Bio-Rad’s Expert Care Service
A certified service engineer documents that
instruments are installed and functioning Bio-Rad offers a no-contract Repair Plan and a wide variety of
properly and ensures that instruments will individualized service plans.
generate reliable and reproducible data. ■■  hoose a preconfigured plan or design your own custom plan
C
with MyPlan for maximum flexibility
■■ Use on-site and off-site services as needed
■■  onsult our team of dedicated experts who have direct access
C
to the latest procedures, parts, tools, and factory-trained
engineers to ensure the best service for your instruments

Visit bio-rad.com/support/expertcare for more information.

Care Plan Entitlements • On-site service option • Service center option

Service Complete Support Plan Proactive Plan Performance Plan Extended Coverage Plan
Repairs •• ••
PM1 •• •• ••
Parts (repair) •• ••
Shipping2 • • •
Labor3 •• •• •• ••
Travel • • • •
Priority response4 •• ••
Remote diagnostics5 •• •• ••
Technical support •• •• •• ••
Instrument check ••
Loaner • •
Part discount6 ••
1
The number of preventive maintenance (PM) visits will be in line with manufacturer’s recommendations. Additional PM visits can be added at agreed-upon price levels. PM is not available in all
locations. 2 Shipping paid by Bio-Rad (not available in all regions). 3 Labor and travel covered for preventive maintenance and performance check activities only with the Proactive and Performance Plans,
respectively. 4 Priority response guaranteed over non-contract customers. 5 Go to bio-rad.com/support/RemoteDiagnosticServices to learn more about remote service and support. 6 Discount
applies for service performed by Bio-Rad outside of the PM service. Note: Services may vary by region. Please contact your local Bio-Rad representative for details of availability.

6 | BIO-RAD LABORATORIES, INC.

CONFIDENCE IN RESULTS

RELIABLE PERFORMANCE. REPRODUCIBLE RESULTS.

From the solid-state optical technology that allows our real-time PCR detection systems
to perform optimally year after year to the algorithms that ensure that all of the most
relevant gene targets are represented in each PrimePCR Panel, our real-time PCR portfolio
provides you confidence in reagents, technology, and results.

Expertise
20 YEARS IN REAL-TIME PCR 30+ YEARS IN PCR

Reliability
1,000s OF CFX SYSTEMS INSTALLED IN 80 + COUNTRIES

Innovation
#1 #2 MARKET LEADER IN DIGITAL PCR MARKET LEADER IN REAL-TIME PCR

1,000s OF Sso7d PUBLICATIONS

BIO-RAD LABORATORIES, INC. | 7

Ordering Information
Catalog # Description Catalog # Description
Instruments Software
1845075  CFX Automation System II for Real-Time PCR Systems, 12004110 CFX Maestro Software, Windows Edition
includes plate handler, barcode scanner, mounting plate, 12004128 CFX Maestro Software, Mac Edition
automation software 12005259 CFX Maestro Software, Security Edition, 5 licenses
1855195 CFX96 Touch Real-Time PCR Detection System,
includes C1000 Touch Thermal Cycler Chassis, CFX96 Optical Visit bio-rad.com/web/qPCRHighThroughput
Reaction Module, cables
1855485 
CFX384 Touch Real-Time PCR Detection System,
for more information.
includes C1000 Touch Thermal Cycler Chassis, CFX384 Optical
Reaction Module, cables Want to order custom PCR primer or probe assays?
1854095 CFX96 Touch Deep Well Real-Time PCR Detection System, Let our search tools find the right assay for you!
includes C1000 Touch Thermal Cycler Chassis, CFX96 Deep Well
Optical Reaction Module, cables Visit bio-rad.com/web/PrimePCR for more information.
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, plate
support block that holds 96- and 384-well plates, sealing frame, Bio-Rad, Hard-Shell, and Microseal are trademarks of Bio-Rad Laboratories, Inc. in
power cord certain jurisdictions.
Reagents and Consumables
Hard-Shell Plates are covered by one or more of the following U.S. patents or their
1725085  SingleShot SYBR® Green Kit for Cell Lysis and RT-qPCR,
foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977;
100 x 50 µl reactions
6,340,589; and 6,528,302.
1725090 
SingleShot Probes Kit for Cell Lysis and RT-qPCR,
SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. is
100 x 50 µl reactions
licensed by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for
1725271 
SsoAdvanced Universal SYBR® Green Supermix, 5 x 1 ml vials,
use in real-time PCR, for research purposes only.
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,
SYBR® Green I, universal reference dye, for 500 x 20 µl reactions All trademarks used herein are the property of their respective owner.
1725281 
SsoAdvanced Universal Probes Supermix, 5 x 1 ml vials,
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,
stabilizers, universal reference dye, for 500 x 20 µl reactions
1725151 iTaq Universal SYBR® Green One-Step Kit, 5 x 1 ml vials, one-
step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix
containing iTaq DNA Polymerase, buffers, and universal reference
dye; iScript Reverse Transcriptase; and nuclease-free water,
for 500 x 20 µl reactions
12010220 
Reliance One-Step Multiplex Supermix, 5 ml (5 x 1 ml vials),
1,000 x 20 μl reactions, 4x RT-qPCR supermix, contains Reliance
Reverse Transcriptase, blend of high-performance DNA polymerases
including Sso7d fusion polymerase, universal reference dye,
stabilizer, and reaction buffer, for 1,000 x 20 µl reactions
12010221 
Reliance One-Step Multiplex Supermix, 10 ml (2 x 5 ml vials),
2,000 x 20 μl reactions, 4x RT-qPCR supermix, contains Reliance
Reverse Transcriptase, blend of high-performance DNA polymerases
including Sso7d fusion polymerase, universal reference dye,
stabilizer, and reaction buffer, for 2,000 x 20 µl reactions
MSB1001 
Microseal 'B' Adhesive Seals, optically clear seals for
PCR plates, 100
HSP3905 
Hard-Shell 384-Well PCR Plates, thin-wall, skirted, clear shell/
white well, barcoded, 50
HSP9901 Hard-Shell 96-Well PCR Plates, low-profile, thin-wall, skirted,
white shell/clear well, barcoded, 50
HSP9955 Hard-Shell 96-Well PCR Plates, low-profile, thin-wall, skirted,
white shell/white well, barcoded, 50
1845098 CFX Qualification Plate, predispensed 96-well plate for
performance validation
1845099 CFX Qualification Plate, predispensed 384-well plate for
performance validation

Bio-Rad
Laboratories, Inc.

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