Professional Documents
Culture Documents
net/publication/324162843
CITATION READS
1 2,365
1 author:
David Perpiñán
Naturavets
45 PUBLICATIONS 358 CITATIONS
SEE PROFILE
Some of the authors of this publication are also working on these related projects:
Revision of the bee group Anthophora (Micranthophora) (Hymenoptera: Apidae), with notes on potential conservation concerns and a molecular phylogeny of the
genus View project
All content following this page was uploaded by David Perpiñán on 25 September 2018.
CPD article
David Perpiñán DVM, MSc, PhD, Dip ECZM (Herpetology), RCVS Specialist in Zoo & Wildlife Medicine, Naturavets
Consultancy, Stroud
A
naesthesia of reptiles can be performed in clinical crocodile has the mouth open under water; the gular fold needs to
practice using the same equipment used for other be displaced ventrally in order to access the glottis for intubation
exotic species, although some unique anatomical (Murray, 2006).
and physiological features need to be taken into The tracheal rings are incomplete in lizards and snakes, and
consideration. Analgesia is a developing field in reptile medicine and complete in chelonians and crocodilia. Therefore uncuffed
recent years have seen significant advances in the understanding of endotracheal tubes should be used in the latter, while either
pain and its treatment in herpetological patients. Evidence-based cuffed or un-cuffed tubes can be used in lizards and snakes.
knowledge is paramount when using analgesics, as drugs and doses Dead space should be minimised; small reptiles can be intubated
used in other veterinary species may not work in reptiles. using intravenous catheters. The trachea is particularly short in
chelonians (Figure 6).
Review of anatomy and physiology Most snakes have one functioning lung, the right one; many
Lizards and snakes both belong to the order Squamata have a second lung but it is usually rudimentary.
and are very similar in many anatomical and physiological Most reptiles function as poikilothermic organisms: their body
aspects, while chelonians (tortoises, turtles and terrapins) and temperature varies considerably, depending on their environmental
crocodilians belong to separate orders, the Testudines and temperature. Therefore anaesthetic drug metabolism, as any
Crocodilia respectively. other body function in these species, depends on environmental
Reptiles do not possess a diaphragm (Figure 1), and air temperature. For a successful anaesthetic event, reptiles should be
movement depends on the abdominal, pectoral and intercostal at their preferred temperature or they will not be able to metabolise
muscles. Therefore the correct term is ‘coelomic cavity’ in reptiles, the drug properly, which can lead to increases in both induction
rather than thoracic cavity and abdominal cavity. Reptiles have time and time to recovery. Respiration in reptiles varies depending
higher pulmonary capacity (volume) than mammals, but the on O2 and CO2 blood concentrations, and ventilation increases in
surface available for gas exchange is not as high as in mammals, situations of hypoxia and hypercapnia (Murray, 2006; Sladky and
because the caudal portion of the lung has limited gaseous Mans, 2012b; Bertelsen, 2014; Vigani, 2014).
exchange function and is used mainly as an air sac in many Respiration is pulmonary in most reptiles, but some aquatic
species (Figure 2). Some species of snakes and lizards inflate their species also perform cutaneous, pharyngeal and cloacal respiration.
lungs considerably, using this technique to look bigger in front These aquatic reptiles can also switch to anaerobic metabolism,
of enemies or predators. The reptile lung is hollow in structure and can spend hours under water without breathing. Inducing
(Figure 3); this can facilitate endoscopy for examination and anaesthesia in these species using gaseous agents via face mask or
© 2018 MA Healthcare Ltd
sample collection (Murray, 2006; Bertelsen, 2014). induction chamber is not practical (Murray, 2006; Vigani, 2014).
Reptiles do not have an epiglottis and the glottis is easily visible Ventricular separation is more complete in crocodilia, followed
in snakes and lizards (Figure 4), less so in chelonians. The glottis by monitor lizards, snakes and other lizards, and then chelonians.
of Crocodilia is behind a gular fold (Figure 5), the function of Even those reptiles with only one ventricle possess cardiac folds
which is to prevent the water from entering the trachea when the that allow for a variable degree of separation between venous and
Liver
Figure 3. Necropsy image of a lizard where the lung has been Figure 6. Necropsy image showing the short distance between
incised, showing the characteristic parenchyma of this hollow the bifurcation of the trachea and the tip of the nose in a tortoise.
structure. Special attention should be paid to avoid intubation of a bronchium.
arterial blood; together with anaerobic metabolism, venous and during anaesthesia to provide adequate ventilation and to monitor
arterial blood mixing explains why some reptiles can spend hours cardiac function. Reptiles should always be kept at their preferred
without breathing in some circumstances. However, even aquatic temperature, and similar to the heart and respiratory rates,
reptiles should be ventilated during anaesthetic procedures in obtaining a cloacal temperature before the anaesthetic starts may
order to avoid complications and delayed recovery (Murray, 2006; help later to monitor this parameter (Sladky and Mans, 2012b).
Schumacher and Yelen, 2006; Sladky and Mans, 2012b). The patient should be fasted ideally for 24–72 hours, and medium
There has been controversy about the renal portal system in to large snakes should be fasted for at least 72 hours before an
reptiles. Initially, it was thought that any drug injected into the elective anaesthetic, as they are prone to vomiting.
caudal portion of the animal was directed first to the kidneys, Dehydration should be corrected before the anaesthetic event.
being eliminated in some cases, and producing toxicity in some Fluid requirements in reptiles are estimated as 15–30 ml/kg/day,
others (Sladky and Mans, 2012b). However, studies showed that plus the percentage of dehydration (Sladky and Mans, 2012b). If
when patients were properly hydrated the effect of the renal an intravenous (IV) or intraosseous (IO) catheter can be placed,
portal system was barely significant (Holz, 1999). More recent 5 ml/kg/h can be given during surgery, but for no more than
studies have shown that drugs may behave differently depending 3 hours. Overhydration is a real danger in small exotic species.
on the species, with no clear pattern: some studies have found Hartmann’s or a 50:50 mixture of 2.5% dextrose and 0.45% saline
no difference between injecting in the front and hind limb provide a fluid type most similar to the osmolarity of reptiles. All
(Campagnol et al, 2014) and some others have found differences, fluids should be administered warmed, at 25–35°C (Sladky and
which may be clinically significant or not (Giorgi et al, 2015; Mans, 2012b).
Kummrow et al, 2008). Current recommendation would be to give
intramuscular injection in the cranial half of the body, although Anaesthetic induction
injection in the caudal half of the body is expected to be effective Anaesthetic drugs can be administered through several routes. The
in many cases. intramuscular (IM) route is commonly used; snakes are injected
in the epaxial muscles, between the scales, while chelonians can be
Pre-anaesthetic assessment injected in the muscles of the cranial aspect of the femur or in the
As in any other animal, a health assessment should be performed large muscles between the plastron and the scapula. Lizards are
before any anaesthetic procedure. This should include a complete commonly injected in the cranial aspect of the femur. Intravenous
physical examination and may also include bloodwork and administration of anaesthetics can be carried out through an
radiographs at the veterinary surgeon’s discretion. In some cases, IV catheter, although they are not particularly easy to place.
if owner’s finances or blood volume limit potential tests, then the Practically, IV catheters are only used in the neck of tortoises
determination of uric acid and packed cell volume are useful for (Figure 8), and an incision of the skin followed by dissection in
identifying conditions such as dehydration, oxygenation problems, order to find the jugular vein is usually necessary in most species.
anaemia, infection and renal failure. It can be useful to obtain a An alternative way to maintain constant intravenous access is
heart rate and a respiratory rate with the animal at rest before the to insert a needle or a butterfly catheter into a vein and secure
anaesthetic (Figure 7); these values will be very important later it in place (Hernandez-Divers, 2006; Murray, 2006; Schumacher
and Yelen, 2006; Sladky and Mans, 2012b). Regular intravenous
injections are also appropriate for injection of anaesthetic drugs
(Figure 9), although the subcarapacial vein in turtles should
be avoided when possible due to reports of serious side effects,
such as paralysis, probably due to injection of the anaesthetic
into the cerebrospinal fluid instead of the subcarapacial vein
(Innis et al, 2010).
Intraosseous catheters can also be placed in lizards and
chelonians. In lizards, it is relatively easy to place these in the tibia,
but in chelonians they should be placed in the humerus, which
is a significantly more complicated procedure (Hernandez-Divers,
2006). There is a study in tortoises demonstrating that fluids are
only well distributed when given through a jugular catheter and, to
a lesser degree, when given IO in the humerus. Injection into the
carapace does not distribute fluids very well (Young et al, 2012).
The intracardiac route is occasionally used in snakes due to the
difficulty in finding the caudal vein for IV administration in some
© 2018 MA Healthcare Ltd
Propofol
Propofol is a short-acting anaesthetic agent that can be given
IV or IO. Doses depend on the size of the animal, but for very
large reptiles 3 mg/kg is used, and for smaller reptiles 10 mg/kg is
more appropriate. Propofol should be given by slow intravenous
injection. If given too fast, the drug will leak out of the vein.
Propofol can also be used for maintenance, but it is far more
common to use a gaseous anaesthetic agent for that purpose.
However, doses for maintenance are 0.5–1 mg/kg as boluses or
0.3–0.5 mg/kg/min as a constant rate infusion. As with any other
anaesthetic agent, propofol produces cardiorespiratory depression
and hypotension. Care should be exercised when propofol,
and probably other injectable anaesthetics, are given in the
subcarapacial vein in chelonians, as paralysis has been reported
as an adverse effect, probably due to the injection of propofol in
the cerebrospinal fluid. Propofol has been used successfully in
small crocodilia, but larger crocodilia need specific protocols that
are beyond the scope of this article (Schumacher and Yelen, 2006; Figure 9. Intravenous administration of alfaxalone in the ventral tail vein of a
Sladky and Mans, 2012b; Keller, 2015). bearded dragon (Pogona vitticeps).
zzThe shelf life once the bottle has been openened is significantly 2013; Knotek, 2014; West, 2017).
longer than for propofol
zzAlfaxalone can be given IM, which is a great advantage in many Ketamine plus alpha-2 agonist
species of reptiles such as snakes, small individuals, dangerous When alfaxalone is not available, a combination of ketamine
animals, etc. and an alpha-2 agonist may be used; this has been extensively
reported in the literature (e.g. Campagnol et al, 2014). Recovery local anaesthetics, particularly in small reptiles. Intubation is
from combinations of ketamine and alpha-2 agonists, particularly strongly recommended for medium-duration to long procedures,
in snakes and lizards, are more prolonged than with propofol, as reptiles do not breathe very well once they are anaesthetised.
alfaxalone or inhalant anaesthetics. In chelonians, the IM or SC Intubation helps the clinician to provide adequate ventilation and
combination of ketamine (10 mg/kg) + medetomidine (0.1 mg/ oxygenation, and facilitates anaesthetic monitoring.
kg) + morphine (1.5 mg/kg) has been used for anaesthesia, with
reversal of the morphine with naloxone (0.2 mg/kg) and of the Gaseous anaesthetic maintenance
medetomidine with atipamezole (0.5 mg/kg) once the procedure Isoflurane at 2–2.5% is appropriate for maintenance, although
has been completed; the effect of this combination is longer than those levels should be decreased with time over the anaesthetic
with the use of propofol or alfaxalone alone, but it can be used procedure to avoid excessive depth of anaesthesia. If additional
for the same kind of procedures (Hernandez-Divers et al, 2009). analgesia is required for a surgical procedure, bupivacaine can be
Many other protocols using different drugs have been described used as a local anaesthetic agent, to reduce the amount of gaseous
in reptiles. anaesthetic agent needed (Bertelsen, 2014).
Figure 12. Palpebral and corneal reflexes cannot be assessed in snakes and
some geckos, as the eyelids of these species are transparent and fused together
forming a spectacle. The spectacle of this snake has turned blue due to
impending shedding.
Figure 11. Induction of an Indigo snake (Drymarchon couperi) using
gas in a ziplog bag. A small opening is left to introduce the gas,
but once the bag is full of anaesthetic it can be closed completely.
softshell turtles) the probe is placed directly over the area of the
heart. Significant decreases in heart rate should raise concern.
The heart of reptiles can continue beating after death, so weak,
slow Doppler signals may not necessarily mean the animal is alive
(Sladky and Mans, 2012b; Vigani, 2014).
Monitoring oxygenation is a bit more difficult in reptiles.
Pulse oximetry, capnography and blood gases have not been
validated in reptiles, and we may only use them to assess trends
(Sladky and Mans, 2012b). While there have been suggestions
to keep capnograph values in reptiles at 15–25 mmHg, these
recommendations have not been based on scientific studies
(Hernandez-Divers et al, 2005). Therefore, the best option to
ensure adequate oxygenation is to provide appropriate ventilation.
Temperature should also be monitored, ideally with a cloacal
probe. The patient’s body temperature can be maintained with
heat mats, forced air warming devices (e.g. Bair hugger®) or bottles Figure 13. In chelonians, the Doppler probe should be placed in the area
or gloves filled with hot water (Figure 14). Hypothermia increases between the neck and the front limb, aiming to the heart.
the risk of anaesthetic complications and prolongs recovery.
Blood pressure studies are still in their infancy in reptiles
(Schumacher and Yelen, 2006; Sladky and Mans, 2012b).
Anaesthetic recovery
Recovery may take a long time in reptiles, therefore it is good
practice to cease providing inhalant anaesthetic before finishing
the procedure, and to start reducing ventilations and changing
oxygen for room air (e.g. using a small Ambu bag). This may
stimulate reptiles to recover faster. It is still controversial whether
changing oxygen for room air speeds recoveries; some studies
have shown a benefit, some others have not. After completing
*
© 2018 MA Healthcare Ltd
Anaesthetic complications
Complications such as bradycardia, bradypnoea and loss of heart
rate can be treated with the same drugs used in small animals.
Changes in reptilian heart rate are usually gradual, therefore
careful monitoring enables action to be taken to correct abnormal
rate and hence avoid cardiac arrest.
zzFor respiratory depression, doxapram can be given IV at
5 mg/kg
zzAdrenaline (epinephrine) is indicated for cardiac arrest or
bradycardia: 0.1 mg/kg IV (dilution 1:1000 = 1 mg/ml). If used
intratracheally, the dose should be doubled and diluted with
saline
zzAtropine should be used for bradycardia: 0.04 mg/kg IV
or IO, or 0.2 mg/kg IM or SC (Carpenter, 2005; Funk and
Diethelm, 2006).
Figure 16. Traumatic injury to a gopher tortoise (Gopherus
polyphemus) caused by a road traffic accident. As the structure of
Analgesia the carapace contains bone, veterinary surgeons should assume
Analgesia in reptiles is a developing science. In some cases, these wounds are painful.
antialgid postures (Figure 15) can help us identify pain in reptiles;
in other cases, veterinary surgeons will need to assume that if a (Sladky and Mans, 2012a).
procedure is painful for people it is painful for reptiles (Figure 16) Tramadol (5–10 mg/kg PO q48–72 hours) has also been used
(Sladky and Mans, 2012a). Different groups of reptiles tend to successfully in chelonians and is a good option for treatments at
respond differently to a given analgesic drug. Drugs typically used home by the owners, as it can be given orally and is not as heavily
to control pain in small animals may not be effective in reptiles; controlled as opioids. Intramuscular administration of tramadol
this is the case for buprenorphine and butorphanol, for example. is also effective (Duvall, 2017). There have been pharmacokinetic
Opioids have been shown to be the best analgesics for reptiles. studies of meloxicam in reptiles, but without functional studies
Morphine (1.5–5.0 mg/kg SC q24 hours), pethidine (10–50 mg/ it is unknown if the drugs works as an analgesic in reptiles.
kg SC or IM q2–4 hours), hydromorphone (0.5–1.0 mg/kg SC/IM While doses for other NSAIDs can also be found in books and
q24 hours) and methadone (3.0–5.0 mg/kg SC/IM q24 hours) have other sources, no scientific study has proven their efficacy. A
been used in chelonians with positive results, and morphine at the recent article in snakes determined that COX-2 was not involved
same dose has been used in lizards also with positive results. The in inflammation, while COX-1 did increase with inflammation;
higher the dose, the more analgesic effect and the more respiratory therefore, at least in snakes, selective COX-2 inhibitors may not be
depression will be seen. Morphine was not effective in snakes, and effective (Sladky and Mans, 2012a; Sadler et al, 2016).
unfortunately, few other studies have been performed in snakes
Conclusions
Reptiles can be anaesthetised using just a few protocols (mainly
involving isoflurane/sevoflurane, propofol and alfaxalone), but
knowledge of anatomy and physiology, as well as specific methods
to apply, maintain and monitor anaesthesia, are necessary for a
successful outcome. Reptile analgesia is a developing science;
doses and drugs applied in other animals cannot be extrapolated
to reptiles, and veterinary surgeons should be aware of specific
scientific studies in order to relief pain effectively in these patients.
CA
References
Bertelsen MF. Squamates (snakes and lizards). In: West G, Heard D, Caulkett N,
editors. Zoo Animal and Wildlife Immobilization and Anesthesia. 2nd edition.
Ames, IA: Wiley Blackwell; 2014. p351–363
© 2018 MA Healthcare Ltd
Campagnol D, Lemos FR, Fernanda R, Silva ELF, Rossi Júnior JL, Borlini TC.
[Comparison of pharmacological restraint with ketamine and xylazine, admin-
istered intramuscularly in the forelimb or hindlimb, in broad-snouted caiman
juveniles]. Pesquisa Veterinaria Brasileira. 2014; 34(7):675–681. doi: 10.1590/
S0100-736X2014000700012
Figure 15. Antialgid posture in a green iguana (Iguana iguana) after a surgical Carpenter JW. Exotic Animal Formulary. 3rd edition. St Louis, MO: Elsevier Saun-
ders; 2005. p592
correction of an intestinal prolapse.