1/8/2020 USP Monographs: Ascorbic Acid

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Ascorbic Acid

C6H8O6 176.12

L-Ascorbic acid.
L-Ascorbic acid [50-81-7].

» Ascorbic Acid contains not less than 99.0 percent and not more than 100.5 percent of C6H8O6.

Packaging and storage— Preserve in tight, light-resistant containers.

USP Reference standards 11 — USP Ascorbic Acid RS.

Identification—

A: Infrared Absorption 197K .

B: A solution (1 in 50) reduces alkaline cupric tartrate TS slowly at room temperature but more readily upon heating.

Specific rotation 781S : between +20.5 and +21.5 , the optical rotation being measured immediately, following the
preparation of the solution.

Test solution: 100 mg per mL, in carbon dioxide-free water.

Residue on ignition 281 : not more than 0.1%.

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1/8/2020 USP Monographs: Ascorbic Acid

Heavy metals 231 — Dissolve 1 g in 25 mL of water: the limit is 0.002%.

Organic volatile impurities, Method IV 467 : meets the requirements.

Residual solvents 467 : meets the requirements.

(Official January 1, 2007)

Assay— Dissolve about 400 mg of Ascorbic Acid, accurately weighed, in a mixture of 100 mL of water and 25 mL of 2 N
sulfuric acid. Add 3 mL of starch TS, and titrate at once with 0.1 N iodine VS. Each mL of 0.1 N iodine is equivalent to 8.806
mg of C6H8O6.

Auxiliary Information— Staff Liaison : Lawrence Evans, III, Ph.D., Scientist

Expert Committee : (DSN05) Dietary Supplements - Non-Botanicals
USP29–NF24 Page 194
Phone Number : 1-301-816-8389

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