Blood banking and transfusion medicine

Theory lectures schedules

1. Blood group system

ABO SYSTEM
a) Genitic and biochemistry
b) ABO subgroup
c) ABO antibodies
d) Laboratory testing ( ABO grouping )
e) ABO discrepancies

2. Rh .BLOOD GROUP SYSTEM

a) Nomenclature
 Fisher – race terminology
 Wiener terminology
 Rosen field terminology

b) D antigen
c) Rh antibody
d) Other Rh .Antigen (other Rh. System )

3. Collection of donor blood ,processing and storage .

 Type of blood donors
 Criteria for selection of blood donors
 Collection of donor blood
 Processing of donor blood

4. Screening tests for infections transmissible by transfusion

a) Hepatitis virus
 Hepatitis A ( HAV )
 Hepatitis B (HBA )
 Hepatitis C ( HCV )
 Hepatitis D ( HDV )
 Hepatitis E (HEV )
 Hepatitis G ( HGF )
b) Retro viruses
 Human immunodeficiency virus ( HIV I,II )
 Human - T-Cell lymphotrophic virus ( HTLV I,II)

c) Other viruses
 Parvo virus
 Epstien – bar –virus
 Cytomegalo virus
 West nile virus (WNV)

d) Bacterial contamination of blood product as syphilis

e) Parasitic infection as Leishmaniasis , Malaria

5. Compatibility test and pretransfusion testing ( cross – matching )

6. Complication of blood transfusion
a) Acute hemolytic transfusion reaction
b) Delayed hemolytic transfusion reaction
c) Febrile non hemolytic transfusion reaction
d) Allergic transfusion reaction
e) Anaphylactic / anaphylactoid reaction
f) Transfusion related acute lung injury
g) Transfusion related associated circulatory over load
h) Transfusion associated graft versus host disease.

7. Blood bank application of antigen – antibody reaction

a) Immunoglobulin review
b) Antiglobulin technique
c) Antiglobulin reagent
d) Indirect antiglobulin test

Dr . Lubna Alaoshibi