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Introduction to Glycolysis
Now let’s review the high level step by step process for Glycolysis. In the
next section we will be reviewing each of these steps involved in
Glycolysis in detail.
Glycolysis -Step 1
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In this step Glucose is first phosphorylated by a hexokinase enzyme to
produce Glucose-6-
phosphate (G6P). Note that this reaction consumes an ATP and as such is
energy consuming.
By phosphorylating glucose into G6P, the cell keeps the internal
concentration of glucose low,
which maintains osmotic flow of glucose into the cell in a positive fashion
Glycolysis -Step 2
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In the next step, the destabilizing of the molecule in the previous reaction
allows the hexose ring to
be split by aldolase into two triose
sugars: dihydroxyacetone phosophate (DHAP) and glyceraldehyde
3-phosphate (GADP).
Glycolysis - Step 5
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Glycolysis -Step 7
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The 1,3 BPG is now converted to 3-phosphoglycerate (3PG), producing an
ATP
This is the break-even stage, since there are two 1,3BPGs created from
one glucose at the cost of 2
ATP’s, and each 1,3 BPG has now yielded an ATP, for a net change of zero
ATPs
Glycolysis -Step 8
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Glycolysis - Step 9
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Glycolysis - Step 10
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We have only looked at a few functional enzymes out of the thousands
that exist
All of them function using what we suspect is the “lock and key” system
first suggested by Emil
Fisher
The lock represents an enzyme and the key represents the substrate it
acts upon
Both enzyme and substrate
have fixed, complementary conformations that lead to a fit
At the active sites, the enzyme has a specific geometric shape and
orientation that a
complementary substrate fits into perfectly
A more modern understanding suggests that the induced fit model is more
appropriate – enzymes and substrates are flexible and constantly in
motion – vibrating – and their proximity can induce the correct fit, provided they
are a match. Now let’s review the schematics of the Lock and Key Analogy as
shown below.
PART 2
Introduction
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While enzyme names tend to be named for the substrate and reaction,
this can become unwieldy
So enzymes are additionally classified by their function using what is
called an EC code
The Enzyme Commission (EC) is a body of experts affiliated with the
International Union for
Biochemistry and Molecular Biology (IUBMB)
An EC contains 4 codes separated by periods (e.g. 1.2.3.4) where the first
number designates the
class of reaction performed by the enzyme
Top Level EC Codes
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Because IF proteins are very cell-type specific, they can be used to identify
the source of metastatic tumours
During mitosis, IFs are depolymerized; they repolymerize after division is
complete.
Several other proteins have been found to bind IF proteins; these are
called Intermediate Filament Associated Proteins, or IFAPs
Plectin: crosslinks IF to microtubules and the lamin network in
the nculeus
Ankyrin: connects IF to plasma membrane proteins and microtubules
PART 3
Introduction
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From the previous course, you should be aware that each codon (set of 3
bases) in the DNA
sequence for a protein coding gene corresponds to a specific amino acid
Changes in the sequence can result in a change to the amino acid coded
for in that position; even
worse, the resulting codon may code for a STOP signal
Alternately, the deletion or insertion of bases in the DNA can result
in frameshift mutations
Now let's review the Sequence Mutations table listed below that highlights
the sequence mutations
at the first, second and third positions.
Regulatory & Transcription Errors
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Splicing Errors:
Translation Errors:
Translation errors are even less common: approximately 1 x 10-5, or 1
in 100,000 amino acids