CONTENTS

S. No. CHAPTER PAGE NO.

1 Introduction 1-6
1.1 Hydrolases 1
1.2 Lipase 1-3
1.3 Production of Lipase 3-4
1.4 Uses of lipase 4-6
1.5 Aims and objectives 6
2 Review of literature 7-21
2.1 Microbial Sources of Lipases 8
2.1.1 Bacteria 8-10
2.1.2 Fungi 10-12
2.1.3 Yeast 12-13
2.2 Screening of lipase- producing microorganisms 14
2.3 Lipase production and parametric optimization 14-15
2.3.1 Effect of inoculum size and age 15-16
2.3.2 Effects of incubation period, temperature and pH 16
2.3.3 Effects of carbon and nitrogen sources on lipase production 17-20
2.4 Biotechnological Applications of Lipases 20
2.4.1 Food industry 20
2.4.2 Organic chemistry 20
2.4.3 Chiral resolution 20-21
2.4.4 Lipase as biosensor 21
2.4.5 Lipases in bioremediation 21
2.4.6 Detergency and cleaning 21
2.4.7 Paper industry 21
S. No. CHAPTER PAGE NO.
3 Materials and Method 22-23

3.1 Samples collection 22

3.2 Isolation 22
3.3 Screening of the Isolates for Lipase Activity 22
3.4 Lipase Production 22-24
3.5 Enzyme Isolation and purification by Ammonium salt 23
precipitation
3.6 Qualitative determination of lipolytic Activity 23
4 Results and discussion 24-31
4.1 Isolation 24-26
4.2 Production 27
4.3 Qualitative estimation of lipase enzyme by lipase 27
assay titrimetric method
4.3.1 Effect of time on enzyme quality 27
4.3.2 Effect of pH on Enzyme quality 28-29
4.5 Discussion 29-30
5 Conclusion 31
6 Summary 32
7 References 33-36