Professional Documents
Culture Documents
SUNRISE Service Manual
SUNRISE Service Manual
SUNRISE
RC, TW, TS, TC, BC
2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
WARNING
CAREFULLY READ AND FOLLOW THE INSTRUCTIONS
PROVIDED IN THIS MANUAL BEFORE OPERATING THE
INSTRUMENT.
Notice
Every effort has been made to avoid errors in text and diagrams, however,
TECAN Austria Ges.m.b.H. assumes no responsibility for any errors which may
appear in this publication.
It is the policy of TECAN Austria Ges.m.b.H. to improve products as new
techniques and components become available.TECAN Austria Ges.m.b.H.
therefore reserves the right to change specifications at any time.
We would appreciate any comments on this publication.
TECAN Austria Ges.m.b.H.
Untersbergstraße 1A
A-5082 Grödig/Salzburg
AUSTRIA / EUROPE
Telephone: 0043 (0)6246/8933
FAX: 0043 (0) 6246/72770
E-mail: office.austria@tecan.com
Copyright Information
The contents of this manual are the property of TECAN Austria Ges.m.b.H and
are not to be copied, reproduced or transferred to another person or persons
without our prior written permission.
Copyright TECAN Austria Ges.m.b.H
All rights reserved.
Printed in Austria.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3
Warnings, Cautions and Notes
There are three types of informational notices used in this manual. These
notices highlight important information or warn the user of a potentially
dangerous situation. The following notices are:
Caution
Indicates a possibility of instrument damage or data loss if
instructions are not followed.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY,
LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS
ARE NOT FOLLOWED.
4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
Table of Contents
1. General
1.1 Introduction ............................................................................... 1-1
Abbreviations:.............................................................................. 1-1
1.1.1 Available Options for SUNRISE .................................................. 1-2
1.2 Instrument Description ............................................................. 1-3
1.2.1 Remote Control ........................................................................... 1-3
1.2.2 Touchscreen................................................................................ 1-3
1.2.3 Back Panel Connections ............................................................. 1-4
1.3 Filter Carriage Description ....................................................... 1-5
1.3.1 SUNRISE Standard Filter Carriage ............................................. 1-5
1.3.2 Sunrise Tuneable Wavelength Filter Carriage............................. 1-5
1.3.3 Software Features ....................................................................... 1-6
1.4 Instrument Features .................................................................. 1-6
1.4.1 Measurement Modes................................................................... 1-6
1.4.2 Microplate Shaking...................................................................... 1-7
1.5 Instrument Accessories............................................................ 1-7
Computer Software for Personal Computer ................................ 1-7
2. Installation Procedure
2.1 Introduction ............................................................................... 2-1
2.2 Unpacking and Inspection........................................................ 2-1
2.2.1 Unpacking Procedure.................................................................. 2-2
2.3 Power Requirements................................................................. 2-2
2.4 Environmental Requirements................................................... 2-2
2.5 Instrument Installation Procedure ........................................... 2-3
2.5.1 Installation of Instrument Control Software.................................. 2-3
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5
Plate Movements....................................................................... 3-15
Duration test .............................................................................. 3-15
3.5 Sunrise Diagnosis Tool .......................................................... 3-16
3.5.1 Installation of Sunrise Diagnosis Tool ....................................... 3-16
3.5.2 Connect instrument ................................................................... 3-17
3.5.3 Get Diagnosis Information ......................................................... 3-18
3.5.4 Duration test .............................................................................. 3-18
3.5.5 Print report................................................................................. 3-19
3.6 Error messages ....................................................................... 3-19
5. Optical System
5.1 Introduction ............................................................................... 5-1
5.2 Optical System Description...................................................... 5-1
5.3 Optical System Diagram ........................................................... 5-2
SUNRISE ST (Option Standard Optic) Instrument ...................... 5-2
SUNRISE TW (Tuneable Wavelength Optic) Instrument ............ 5-2
5.3.1 Measurements with Wavelengths below 400 nm ........................ 5-3
5.3.2 Agglutination Measurement Mask for SUNRISE ST Option........ 5-3
5.4 Lamp Replacement ................................................................... 5-4
5.5 Filter Slide .................................................................................. 5-5
5.5.1 SUNRISE Filter Slide (standard filter slide) ................................. 5-5
5.5.2 Replacement of Filters in SUNRISE Filter Slides ........................ 5-6
5.5.3 Filter Coding for SUNRISE Filter Slides ...................................... 5-7
5.5.4 Definition of the filter slide codes:................................................ 5-8
Slide D......................................................................................... 5-8
5.6 Filter slide ( Tuneable wavelength).......................................... 5-9
5.7 Lamp and Mirror Unit Parts .................................................... 5-11
5.7.1 Heat Filter and Air Filter ............................................................ 5-11
5.7.2 Mirror, Optical Lens ................................................................... 5-12
For Mirror Replacement: ........................................................... 5-12
For Optical Lens Replacement .................................................. 5-13
5.8 Filter Guide (standard instrument ) ....................................... 5-13
5.9 Filter Guide ( Tuneable Wavelength): .................................... 5-15
5.10 Fiber Bundle ............................................................................ 5-16
5.10.1 Difference between Standard Fiber Optic and Tuneable Wavelength
Fiber Optic................................................................................. 5-17
5.11 Further Parts belonging to the Optical System .................... 5-17
5.12 Blocking Filter Wheel for Tuneable Wavelength .................. 5-18
5.13 Filter Wheel Properties ........................................................... 5-19
5.14 Mirror for Barcode Scanner.................................................... 5-19
6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
6. Mechanical System
6.1 Introduction ............................................................................... 6-1
6.2 Transport System Description ................................................. 6-1
6.3 Measurement Procedure .......................................................... 6-1
Inside Shaking............................................................................. 6-2
6.4 The Transport Box Assembly .................................................. 6-2
6.5 Exchanging the Transport Box ................................................ 6-3
6.6 Transport Box Adjustments ..................................................... 6-5
6.7 Replacing the Temperature control unit ................................. 6-5
7. Electronic System
7.1 Introduction ............................................................................... 7-1
7.2 Interconnection diagram (standard RC).................................. 7-2
7.3 Voltage diagram (standard RC)................................................ 7-3
7.4 Main Board ................................................................................. 7-4
7.5 Replacing the Main Board ........................................................ 7-5
7.6 Power Board (standard)............................................................ 7-7
7.7 Exchanging the Power Board (standard) ................................ 7-8
7.8 Power Board (tuneable wavelength)........................................ 7-9
7.9 TW Board ................................................................................ 7-11
7.10 Thermo Board.......................................................................... 7-12
7.11 12 Volt Board ........................................................................... 7-13
7.12 Power Supply board................................................................ 7-14
7.13 Replacing the Power Supply .................................................. 7-15
7.14 Barcode Board......................................................................... 7-16
7.15 Optical Switches...................................................................... 7-17
8. Interfaces
8.1 Computer Interface ................................................................... 8-1
8.1.1 Hardware Specifications.............................................................. 8-1
8.1.2 Pin Designation ........................................................................... 8-1
8.1.3 RS-232-C Interface Lines ............................................................ 8-2
Requirements for the Serial Interface:......................................... 8-2
Description of the serial Interface:............................................... 8-3
Implemented RS-232-C Interface Lines : .................................... 8-3
Available Setups:......................................................................... 8-3
8.2 Handshakes: .............................................................................. 8-4
Software Handshake: .................................................................. 8-4
8.3 Commands Response Time: .................................................... 8-4
8.4 Command Syntax for the Serial Interface: .............................. 8-4
Sunrise Mode (Standard): ........................................................... 8-4
Spectra Mode (for compatibility):................................................ 8-4
8.5 Synchronization and Data Format ........................................... 8-5
8.6 Signal Levels ............................................................................. 8-5
8.7 Software Specifications ............................................................ 8-6
8.8 Handshake ................................................................................. 8-6
8.9 Timing Of Messages ................................................................. 8-6
8.10 Communication Messages ....................................................... 8-6
8.11 Printer Interface......................................................................... 8-7
8.11.1 Character Set Setting .................................................................. 8-7
8.11.2 Hardware Specifications.............................................................. 8-7
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7
8.11.3 Connector.................................................................................... 8-7
8.12 Parallel Interface Lines ............................................................. 8-8
8.13 Table of Connections ................................................................ 8-8
8.14 Key to Table ............................................................................... 8-9
Connector Pin Signals................................................................. 8-9
Strobe.......................................................................................... 8-9
Data 1 - Data 8 ............................................................................ 8-9
Acklng - Acknowledge ................................................................. 8-9
Busy ............................................................................................ 8-9
8.15 Connection with Touchscreen interface connector............. 8-10
8.15.1 XChange Files:.......................................................................... 8-10
10. Maintenance
10.1 Instrument Disinfection .......................................................... 10-1
10.2 Disinfection Solutions ............................................................ 10-1
10.3 Disinfection Procedure ........................................................... 10-2
10.4 Disinfection Certificate ........................................................... 10-2
10.5 Preventative Maintenance Plan for SUNRISE Instruments . 10-3
10.5.1 Daily .......................................................................................... 10-3
10.5.2 Weekly....................................................................................... 10-3
10.5.3 Every Six Months ...................................................................... 10-3
10.5.4 Yearly (Service Technician Required) ....................................... 10-3
10.5.5 Every Four Years ...................................................................... 10-3
10.6 Electrostatic Discharge Information...................................... 10-4
10.6.1 Generating Static....................................................................... 10-4
10.6.2 Preventing Electrostatic Damage to Equipment ........................ 10-5
10.6.3 Personal Grounding Methods.................................................... 10-5
8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
11. Performance Testing / Quality Control
11.1 Introduction ............................................................................. 11-1
11.2 Operating for Maximum Performance ................................... 11-1
11.2.1 Instrument Location................................................................... 11-1
11.2.2 Operating Procedure ................................................................. 11-2
11.2.3 Self Check Procedure ............................................................... 11-2
11.3 Performance Tests .................................................................. 11-2
11.3.1 QC PAC 2.................................................................................. 11-3
11.3.2 Microplate Test.......................................................................... 11-3
11.3.3 High Meniscus Liquids .............................................................. 11-4
Agglutination Method................................................................. 11-5
Manual Method.......................................................................... 11-6
Example .................................................................................... 11-6
11.4 Quality Control Testing........................................................... 11-7
11.4.1 Precision Testing....................................................................... 11-7
11.4.2 Instrument Accuracy.................................................................. 11-8
11.4.3 Instrument Linearity................................................................... 11-9
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9
1. General
1. General
1.1 Introduction
SUNRISE Remote Control is intended for use with only external software.
Abbreviations:
RC Remote control
TW Tuneable wavelength option
TS Touchscreen option
TC Temperature control option
BC Barcode option
Results obtained using the SUNRISE are influenced by the proper use of
the instrument, according to the instructions given in this manual, as well
as the liquid compounds used (reagents, chemistry). The instructions for
use, storage and other manipulations in connection with samples or
reagents have to be strictly followed. Taking this fact into consideration,
results must be interpreted carefully.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-1
1. General
Touchscreen
B037302
SUNRISE
Remote Controlled
Tuneable wavelength
from 400 – 700 nm
B037306
Temperature control
B037303
Barcode scanner
B037304
CAUTION
IF THE INSTRUCTIONS GIVEN IN THIS MANUAL ARE NOT CORRECTLY
CARRIED OUT, THE INSTRUMENT MAY EITHER BECOME DAMAGED OR
MAY NO LONGER BE ABLE TO PERFORM ITS PROCEDURES
CORRECTLY AND THE ACCURACY OF THE INSTRUMENT
CAN NO LONGER BE GUARANTEED.
1-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
1. General
Power ON LED
Lamp Compartment
1.2.2 Touchscreen
Touchscreen
Sunrise
Memo Card
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-3
1. General
TECAN TECAN
A-5082 AUSTRIA
CAUTION LASERSTRAHLUNG
NICHT IN DEN STRAHL BLICKEN
TYPE Sunrise
LASER KLASSE 2
ART.-NR. F0393xx VA 110
Leistung: <1mW SER.-NR.
LASER RADIATION - Wellenlänge: 675nm
xxxxx Hz 50 / 60
EN60825-1:1994+A11:1996 VOLTAGE AC 100 - 120 / 220 - 240 MADE IN
DO NOT STARE INTO BEAM M-CODE xxxxxx AUSTRIA
I
ON / OFF Switch LISTED RS 232
0
UL UL
TECAN
PRINTER
R C R
8C64
Mains Socket LABORATORY EQUIPMENT
2 x F2A
All connected devices must be approved and listed as per EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data Processing Devices
1-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
1. General
For more information about the definition of the new and customized filter
slides, see 3.8 Define Filter.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-5
1. General
SUNRISE Remote Control is intended for use with only external software.
Sunrise Touchscreen is a stand alone device, that works with windows CE.
For more information about the software features, see the appropriate
individual manuals. For example: refer to the Magellan Reference manual.
The Center measurement mode should be used if the liquid in the microplate
produces a high meniscus, as an incorrect optical density could be obtained if the
optical density is measured at three positions. If an Agglutination measurement is
performed, all the measurement positions are used.
1-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
1. General
Software Functionality
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-7
2. Installation Procedure
2. Installation Procedure
2.1 Introduction
This chapter contains the necessary information for installing the instrument.
The installation procedures involve unpacking, environmental requirements,
power requirements and interfacing.
Accessory box
Foam Packaging
Foam
Packaging
Instrument
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 2-1
2. Installation Procedure
Connect the instrument only to a electricity supply system with protective earth.
WARNING
TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE
REPLACED WITH THE SAME TYPE AND RATING OF FUSES.
2-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
2. Installation Procedure
WARNING
BEFORE THE INSTRUMENT IS INSTALLED AND SWITCHED ON, IT SHOULD
BE LEFT TO STAND FOR AT LEAST THREE HOURS, SO THERE IS NO
POSSIBILITY OF CONDENSATION CAUSING A SHORT CIRCUIT.
When the requirements above have been met, installation is carried out using the
following procedure:
1. Place the instrument into the required position.
Ensure that the distance between the back panel of the instrument and the
wall, is at least 10 cm.
2. Connect the instrument to the external computer with the required interfacing
cable.
The interfacing cable is connected into the 9 pin serial interface socket, in
the back panel.
3. Ensure that the mains power switch in the back panel of the instrument is in
the off position.
4. Insert the power cable into the mains power socket in the back panel.
5. Switch the instrument on using the mains power switch in the back panel.
The instrument is now ready to measure microplates.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 2-3
3. Firmware and Software Description
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-1
3. Firmware and Software Description
3-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
To Download the new Firmware version, click Open file... and select the correct
*.upd file from the new window. See picture below. Click Download and the new
firmware is Downloaded.
Note:
Do NOT switch off the instrument during the Download process.
When the Download process is finished, the right window displays the Download
Report and the message, "Firmware Download successfully finished!".
Select Print Report from the <File> menu to print the Download Report.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-3
3. Firmware and Software Description
3-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
Select the correct communication port and the baudrate. Click Next.
To use the instrument with software designed for former TECAN readers, select
the appropriate instrument modes and baudrate. Click Next.
Sunrise mode It is recommended to use the SUNRISE mode with 9600 baud.
Spectra mode Simulates a SPECTRA Reader.
Rainbow mode Simulates a Rainbow Reader
ATC mode Simulates an ATC Reader.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-5
3. Firmware and Software Description
Click the Filter slide Out button to move the filter out of the instrument.
To insert a filter slide, open the filter compartment manually and slide the filter
into the slot, so that the filter end of the slide is inserted first. (Do not force the
filter slide into the instrument beyond the point of resistance).
Click the Filter slide In button and the filter is inserted.
Pos1 - 4 show the filter values for the currently loaded absorbance filters.
The instrument is able to recognize predefined filter slides and the filter
values for these slides must not be changed. However, if the filters in the
filter slide have been changed (by a service engineer) or if a new
undefined customized filter slide is to be used, the filter slides need to be
defined.
To define the filter values for a new filter slide, enter the required wavelengths in
the text boxes. Click Next.
3-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-7
3. Firmware and Software Description
Select Connect from the Setup menu or press <Strg> and <F5> together to
connect the instrument.
3-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
The Setup Port dialog box appears. Select <Find any> and click OK
The SunSet Software shows the connection to the reader. The reader type, Serial
No., date and time are displayed:
Select Setup all from the <Setup> menu to adjust the instrument.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-9
3. Firmware and Software Description
Select the first filter and click Start. The dialog box shows the minimum and
maximum values in percent (%). Adjust the three screws until the minimum and
the maximum percentage values lie in the lowest range possible.
Click Poti adjust to adjust the amplification. The software adjusts the potis
automatically and checks that the minimum and maximum percent values are
between a minimum 58 and maximum 89 percent(%).
The following picture shows the three screws for adjusting the mirror:
3-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
Click Poti check again to check and adjust the other filters (adjustment is done
automatically by the software. The values should not be higher than 55 %.)
Make sure the values are correct for all of the available filters on the filter slide. If
the values are within the correct range, click Close to return to the Mirror and
Lamp Adjust dialog box.
If the values are too far apart, and it is not possible to get better results, the fiber
optic must be replaced. See chapter 5.10 Replacing the Fiber Bundle.
Select a filter and click Start to calibrate the plate transport. The <home to fixed
out> and the <home to fixed in> values calibrate the in and out movement.
<home to full dark edge> is a white-dark position on the plate carrier and is
adjusted by the light beam. It counts the steps from the home to the dark edge
position. The <home to rear> shows the number of steps from the home to the
rear plate transport position. This adjustment is required to locate all the wells on
a MTP plate correctly.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-11
3. Firmware and Software Description
Click Start to start the adjustment. The plate carrier moves out and the following
dialog box is displayed:
Insert the Inhouse plate with the “A1” positioned top left and click OK. The
instrument starts the measurement and adjusts all the values.
After the measurement the window shows the offset values. Click OK to return to
the Measurement Offset dialog box and click OK again to go to the next step.
3-12 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
In this dialog box the Options, RC mode and filters can be defined. In the
<Options> area activate the installed options by selecting the appropriate
checkbox (Barcode, Temperature control, Touchscreen).
Caution
If an option is activated that is not available, the instrument will
not function properly!
When using the Tuneable Wavelength option you must enter the filter table
values in the Gradient filter text boxes and you must also enter the Filter slide
ID number of the gradient filter slide. If more standard filters are used, define the
filters under Filter definitions. After all of the information has been correctly
entered, click OK.
The filter slide moves out and the following dialog box is displayed:
Insert the appropriate filter slide and click OK. The filter slide moves in and all of
the defined filters are adjusted. If more filter slides are defined the instrument
adjusts all of the slides. It will request each filter slide in turn, as in the above
dialog box.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-13
3. Firmware and Software Description
• Filter control…
• Plate Movements…
• Duration test…
Filter control
To move the filter slide perform the following procedure:
Select Filter control… from the <Options> menu and the following dialog box is
displayed:
Click Eject to move the Absorbance filter slide out of the instrument.
To insert a filter slide, open the filter compartment manually and place the filter in
the instrument so that the filter end of the slide is inserted first.
Click Insert to move the Absorbance filter slide in. The filters can now be defined
in the User configuration dialog box (Under Setup, select User configuration…)
When all the required movements have been performed, click OK to end.
3-14 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
Plate Movements
To move the plate carrier perform the following procedure:
Select Plate Movements… from the <Options> menu and the following dialog
box is displayed:
To move the plate carrier out, click Eject Plate. To move the plate carrier in, click
Insert Plate. The plate carrier is automatically positioned correctly. When a
measurement is started the plate moves into the instrument.
When all the required movements have been performed, click OK to end.
Duration test
To perform a duration test use the following procedure:
Select Duration test… from the <Options> menu and the following dialog box is
displayed:
Select the number of cycles (e.g. to 200). Click Start to start the duration test.
If the barcode option is activated, a microplate with a barcode can be inserted
and will be used for the Duration test. Enter the Barcode number in the Details:
textbox next to the word 'Barcode:'
When the Duration test is finished, the Status window shows the numbers of
cycles and errors, if any.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-15
3. Firmware and Software Description
Note:
For more detailed information see Chapter 9.3 Sunrise Diagnosis Software
This program enables the user to print out the data regarding the installed
options, adjustment and the last ten errors of the instrument:
• Connect
• Get diagnosis information
• Duration test
• Print report
3-16 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
From the command menu of the Sunrise Diagnosis Tool dialog box select
Connect… from the <Test> menu.
The Setup Port dialog box appears:
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-17
3. Firmware and Software Description
Select the number of cycles (e.g. to 200). Click Start to start the duration test.
If the barcode option is activated, a microplate with a barcode can be inserted
and will be used for the Duration test. Enter the Barcode number in the Details:
textbox next to the word 'Barcode:'
When the Duration test is finished, the Status window shows the numbers of
cycles and errors, if any.
3-18 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
3. Firmware and Software Description
Select Print… from the <File> menu to print the diagnostic data. Select Send as
Mail… to send this information via email.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-19
4. Removing and Replacing the Instrument Cover
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 4-1
4. Removing and Replacing the Instrument Cover
Carefully pull the instrument cover backwards, approx 1-2 cm and lift upwards
to remove.
4-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
4. Removing and Replacing the Instrument Cover
Switch off the instrument and disconnect it from the mains supply.
Remove the lamp compartment cover by gently pulling it from the underside.
Remove the four screws in the right hand side cover area.
Open the plate opening lid and remove the screw in the top left hand corner.
Open the filter compartment lid and remove the screw on the bottom left hand
side.
Ensure that the catch and cover do not become damaged.
POWER
Lamp
Compartment
Cartridge / Filter Compartment Lid Lid
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 4-3
4. Removing and Replacing the Instrument Cover
POWER
Lamp
Compartment
Cartridge / Filter Compartment Lid Lid
4-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
4. Removing and Replacing the Instrument Cover
Carefully pull the instrument cover backwards, approx 1-2 cm and lift upwards
to remove.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 4-5
4. Removing and Replacing the Instrument Cover
4-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
5. Optical System
5.1 Introduction
This chapter contains the description of the optical system and how the parts are
to be exchanged and adjusted. This chapter refers only to the Sunrise
Remote Control Reader. For additional information about options like Tuneable
Wavelength, please refer to Chapter 9: Description of Options.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-1
5. Optical System
Main Board
Transport box
Microplate
Reference
Diode
Channel
Mirror
Optical Lens
Microplate
Reference
Diode Blocking
Channel Filter wheel
Optical Lens Mirror
Heat Absorbing
Filter
Filter Slide Block Lamp
5-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
Agglutination Measurement
Optical density measured up to 40
times across each well
The SUNRISE ST instrument is fitted with a special mask fitted to the lower part
of the transport box so that a very thin beam of light is produced. The
agglutination mask is not available as a spare part. As it is fitted to the transport
box, the transport box has to be ordered in case this part is faulty.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-3
5. Optical System
Lamp Compartment
Remove the lamp compartment cover by gently pulling it from the underside.
Push the locating spring bar, on top of the lamp, to the left.
Carefully remove the lamp from the lamp holder.
To prevent burns, ensure that the lamp is cold.
Disconnect the lamp power cables from the lamp power connector.
Replace the old lamp.
Reconnect the lamp power connector.
Replace the lamp into the lamp holder.
Please note that the lamp is held in place by three lugs, between which the lamp
is to be inserted.
Insert the lamp so that the cables are at the bottom.
5-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
Locating Lugs
Connecting Plug
Caution
Do not touch the reflective surface and the bulb. Any finger prints on these
surfaces must be removed with acetone or methylated spirits.
Caution
After replacing the halogen lamp, select Lamp adjust from the Setup menu
to reconfigure the Mirror & Lamp Adjust settings.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-5
5. Optical System
Rod
Filters
Retaining Rings
Using a wooden or rubber rod (ensure that the ends are rounded so that the
corners do not scratch the filters) carefully push the filter and retaining ring out of
the filter slide.
Turn the filter slide over and insert the new filter and the retaining ring.
Carefully push the filter and retaining ring into the filter slide, using a wooden or
rubber rod.
Ensure that the filter is not scratched.
Replace the springs and screws.
5-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
The first 2 slits are used to define the slide type ( 4-filter or gradient).
The second 6 slits are used to define the filter slide (A-H)
The third (bigger) slide is the home position slide.
The slits in the fourth part of the filter are used to define the position of each filter
fitted in the slide by using the optical switch.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-7
5. Optical System
Slide A: Slide B
Slide C Slide D
Slide E Slide F
Slide G Slide H
5-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
With the gradient filter slide, measurements can be made from 400 nm to 700 nm
using only one filter slide. There are over 300 wavelengths available from one
gradient filter slide. Complete Spectrum scanning - useful for screening new or
unknown compounds. The gradient filter slide makes the instrument highly
flexible.
The following adjustments are necessary for using the gradient filter slide with the
sunset software:
• Select reader type
• Check Serial Number
• Enter the gradient filter values
• Enter Filter slide ID number
• Set Calibration values (determined by the filter slide)
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-9
5. Optical System
The TW filterslide comes delivered with a calibration sheet. This sheet lists the
relationships of the various wavelengths with the position of these wavelengths
on the TW filterslide. In the Gradient filter area, enter the gradient filter values in
the text box next to the appropriate wavelength values. Each TW filterslide has its
own ID number. In the Filter slide ID area, enter this number.
5-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-11
5. Optical System
5-12 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
Sensor
Filterslide-
housing
Fibre Slot
DC Motor
The filter guide is responsible to detect the filter slide (A...H) Refer also to
Chapter 5.5.3 Filter Coding for SUNRISE Filter Slides for detailed information on
filter coding. The filter guide also moves each of the four filters fitted in the filter
slide into the right position.
An optical sensor is used to detect the filter slide as well as the position of the
filters.
A DC motor is used to move the filter slide forward and backward.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-13
5. Optical System
The only serviceable parts are the optical sensor and the whole filter guide.
For replacing these parts please refer to the following procedure:
5-14 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
DC-Servo motor
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-15
5. Optical System
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5. Optical System
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-17
5. Optical System
Unscrew the
two screws on
the bottom
5-18 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
5. Optical System
Light-blue filter
Green filter
Dark-blue filter
Unscrew the
two screws to
remove the
mirror unit.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5-19
6. Mechanical System
6. Mechanical System
6.1 Introduction
This chapter contains the description of the transport system and how the parts
are to be exchanged and adjusted.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 6-1
6. Mechanical System
Inside Shaking
If the microplate is to be measured using inside shaking, the microplate is
moved into the instrument when the measurement is started and the shaking is
performed.
After the shaking has been performed, the plate support is moved until the End
optical switch is detected.
When the End optical has been detected, the microplate is moved under the
measurement diodes and measured after the required number of steps.
After the instrument has completed the measurement, the plate support is
returned to the Start optical switch. The instrument checks that the number of
steps required to reach the Start optical switch is the same as the number of
steps stored in the memory for the calibration length.
If the number of steps are different, a transport error message is displayed and
the measured results are ignored.
The Transport box is a complete unit which contains all the mechanical parts for
the plate transport. This part is not serviceable, if any defects occur the entire
unit must be replaced.
6-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
6. Mechanical System
Front Cover
Mainboard
Interconnectors
Power Board
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 6-3
6. Mechanical System
Note: To avoid damage to the fiber bundle, take care not to bend the fibers
when removing from the transport assembly.
6-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
6. Mechanical System
10. Disconnect the Flex cable from the Thermo Board on the left side and
remove the nozzle plate.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 6-5
6. Mechanical System
11. Remove the Thermo Board and disconnect all cables on the board.
13. Remove the fans and make a note of the position of each fan, so that it can
be returned to the same location
6-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
7. Electronic System
7.1 Introduction
This chapter describes the replacement and adjustment procedures for the
various electronic parts of the instrument.
This chapter also contains the connection diagrams illustrating how the various
parts of the instrument are interconnected.
The instrument contains the following electronic parts:
1. Main Board
This is the major board in the instrument and contains the microprocessor and
the Analog / Digital converter. The A/D Converter is used to measure the light
that is transmitted through the samples. The light is converted into a digital
value that is be used to calculate the optical density of the sample.
2. Power Board (standard)
This board is used to control the transport motor, filter carriage motor, the 5 volt
voltage regulation, the lamp control and for the temperature control of the
instrument (fan).
3. Power Board (tuneable wavelength)
This board has the same function as the standard board, but there is one more
motor driver for controlling the filter carriage motor (DC Servo Motor by TW
Option).There is also a voltage regulation for the TW board.
4. TW Board
This board is for controlling the tuneable wavelength blocking filter wheel.
5. Thermo Board
This board controls the Nozzle-plate, the fans of TC unit, sensors of the TC unit,
the upper heating plate in the transport box and to regulate the Peltier module
of the Temperature control unit.
6. 12-volt board
The board convert the 24 volt input voltage to 24/12 volt output voltage. The 12
volt voltage is to control the Peltier module.
7. Power Supply
This unit is used to supply the various voltages that are required by the
instrument.
8. Barcode Board
This board controls the barcode reader.
9. Optical Switches
This section gives the layout and circuit diagrams for the optical switches that
are used in the instrument.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-1
7. Electronic System
GND
Chassis
Fuses LAMP
Mains Input
ON/OFF Switch
ST 15 ST 23
ST 14
ST 1 ST 8
ST 22 ST 18
Motor
VAN
Filter Transport Mechanism
7-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
+24V
~220V
red black
+24V +2V
black red
+24V
GND
Chassis
+2V
Fuses LAMP
Mains Input
ON/OFF Switch
ST 15 ST 23
ST 14
ST 1 ST 8
ST 22 ST 18
+24V
Motor
red
Filter Carriage Sensor black
+24V
+12V VAN
Filter Transport Mechanism
red black
+12V
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-3
7. Electronic System
Touchscreen
Connector to
Battery connection
RS 232 A/D Converter Area
Connector to
Powerboard
Connector for options
Transport N.C
start position
optical switch
connector
Filter
carriage
sensor
connector
Connector to
Power Supply
Transport end
position optical
switch connector
Connector to
printer interface
7-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
Remove the
three screws
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-5
7. Electronic System
Perform the device configuration with the SunSet software as shown in the
picture.
7-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
Front side:
Connector to
Power on LED
Connector to
Lamp
Rear side:
Connector to filter
transport motor
Connector to
fan
Connector to
power supply
Connector to
transport motor
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-7
7. Electronic System
7-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
Front view:
Rear view:
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-9
7. Electronic System
7-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
7.9 TW Board
This board is for controlling the tuneable wavelength filter wheel.
24 volt power
connector Option connector to
Mainboard
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-11
7. Electronic System
The board controls the nozzle plate, the fans of TC unit, sensors of the TC unit,
the upper heating plate in the transport box and to regulate the Peltier module
of the Temperature control unit.
Connector to heating
plate transport box
Peltier power
connection
Connector to Mainboard
(option connection)
7-12 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
The board convert the 24 volt input voltage to 24/12 volt output voltage. The 12
volt voltage is for controlling the Peltier module.
24 volt power
connection from the
12 /24volt power power supply.
connector to the
thermo board.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-13
7. Electronic System
Connector to
CPU board and
power board
Connector to
Main power
socket
Fuse
7-14 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-15
7. Electronic System
Boot EPROM
7-16 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
7. Electronic System
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7-17
7. Electronic System
Circuit Diagram:
K
GND
GND
OUT
7-18 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
8. Interfaces
8. Interfaces
Pin 5 Pin 1
Pin 9 Pin 6
All connected devices must be approved and listed as per EN 60950, UL 1950
or CSA C22.2 No. 950 for Data Processing Devices
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 8-1
8. Interfaces
The connecting cable used to connect the instrument to the computer should be
wired as given below:
INSTRUMENT COMPUTER
TD connected to RD
RD connected to TD
GND connected to GND
RTS connected to CTS
DSR connected to DCD and DTR
8-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
8. Interfaces
Available Setups:
(default is BOLD)
Baudrate: 300, 600, 1200, 2400, 4800, 9600, 19200, 31250, 38400
Data Bits: 7 or 8
Parity: No, Even, Odd
Stop Bits: 1 or 2
Protocol: No, Hardware (RTS, CTS), Software (XON/XOFF)
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 8-3
8. Interfaces
8.2 Handshakes:
Software Handshake:
The instrument stops transmitting the data, when it receives the 'XOFF' control
code (ASCII Code 19).
The instrument then waits for the computer to send the 'XON' control code
(ASCII Code 17) to start transmitting the data again.
8-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
8. Interfaces
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 8-5
8. Interfaces
8.8 Handshake
This instrument has a software handshake.
The instrument stops transmitting the data, when it receives the 'Xoff' control
code (ASCII Code 19).
The instrument then waits for the computer to send the 'Xon' control code
(ASCII Code 17) to start transmitting the data again.
8-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
8. Interfaces
The printer interface connector is for the R.C Mode Sunrise instrument not
available!!
8.11.3 Connector
The printer connector is a 25 pin connector.
Pin 13 Pin 1
Pin 25 Pin 14
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 8-7
8. Interfaces
1 STROBE out
2 DATA 1 out
3 DATA 2 out
4 DATA 3 out
5 DATA 4 out
6 DATA 5 out
7 DATA 6 out
8 DATA 7 out
9 DATA 8 out
10 ACKLNG in
11 BUSY in
12 PE in
13 SELECT in
14
15 FAULT
16
17
18 GND
19 GND
20 GND
21 GND
22 GND
23 GND
24 GND
25 GND
8-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
8. Interfaces
Strobe
This is a synchronizing output signal so that the printer reads the data.
This is normally 'high' and the data is read when the signal goes 'low'.
The signal must be low for at least 0.5 microseconds.
Data 1 - Data 8
These are the output signals that carry the 8 data bits of information.
The data is read in synchronization with the strobe pulse. A high level indicates
a logical '1'.
The signal must be present 0.5 microseconds before the strobe pulse is
received.
Acklng - Acknowledge
This is an input signal to tell the instrument that the printer is ready to receive
the next block of data.
This signal is sent when the busy signal drops from 'High' to 'Low'.
The signal is automatically sent when the printer is switched on line.
Busy
This is an output from the printer which indicates the status of the printer.
When the signal is high the printer is busy and cannot receive any data. The
signal is high under the following conditions:
1. The data receive buffer is full.
2. The printer is processing the data.
3. The printer is off line.
4. The printer is in an error state.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 8-9
8. Interfaces
Before beginning to upload files, switch the instrument off and plug the
remote serial cable into the port com 1. Switch the instrument on and
upload files to the personal computer.
Make sure that the files that are exchanged are created with the same
version of the Magellan.
8-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
9.1.1 Introduction
In order to perform some of the service and repair adjustment procedures you will
need the Service Tool Kit. It consists of a service plate, spare screws and clips,
the software packages - QC Pac 2 Service, Sunrise Sunset, Sunrise Diagnosis,
RS 232 interface cable, Technical Manual, 3 year warranty labels and a reader
package CD.
Spare
Software: QC Pac 2 screws and
Service, Sunrise clips
Sunset, Sunrise
Diagnosis
Service
Plate
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-1
9. Sunrise Service Tool Kits for Sunrise RC and Options
3 Year
Warranty
Technical Manual
Reader
package CD
9-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
Date: 04/27/2001
Time: 12:21:29
Reader: Sunrise
Unit parameters:
***************************************
* Sunrise - Diagnostic - Information:
***************************************
Serial number: 03930000219
Versions:
FW(Cpu): V 3.13 04/08/00 Installed firmware
Boot loader: V 1.00 BOOT New Version V1.12, Boot EPROM
HW: V 1.00 HW Internal
Config Table: V 1.00 Internal
DEVICE OPTIONS:
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-3
9. Sunrise Service Tool Kits for Sunrise RC and Options
Filter definitions:
Carriage: A
405(30) 450(33) 492(33) 620(34) The numbers in parentheses are the
Carriage: B Poti-values which are defined with
0(30) 0(30) 0(30) 0(30) adjust check in the SunSet program.
Carriage: C 30 is the default (Norm)
0(30) 0(30) 0(30) 0(30)
Carriage: D
0(30) 0(30) 0(30) 0(30)
Carriage: E
0(30) 0(30) 0(30) 0(30)
Carriage: F
0(30) 0(30) 0(30) 0(30)
Carriage: G
405(39) 620(37) 750(33) 340(43)
Carriage: H
0(30) 0(30) 0(30) 0(30)
Carriage: I
340(30) 402(30) 492(30) 450(30) 0(30) 0(30) These values are for a series,
Carriage: J that has not yet been developed;
0(30) 0(30) 0(30) 0(30) 0(30) 0(30) it will be a Sunrise with 6
Carriage: K different filters.
0(30) 0(30) 0(30) 0(30) 0(30) 0(30) The filters will be narrower,
Carriage: L and be closer together, which
0(30) 0(30) 0(30) 0(30) 0(30) 0(30) means the step motor used for
Carriage: M filters A-G will not be able to be used.
0(30) 0(30) 0(30) 0(30) 0(30) 0(30) Instead, a step motor, similar to
Carriage: N the one used with the TW Option,
0(30) 0(30) 0(30) 0(30) 0(30) 0(30) will be necessary.
Carriage: O 30 is still the default (Norm)
0(30) 0(30) 0(30) 0(30) 0(30) 0(30)
Carriage: P
0(30) 0(30) 0(30) 0(30) 0(30) 0(30)
9-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
MEASURMENT:
Transport:
Calibration: VALID
Home to fixed in: 0159 steps From the position where the plate transport is
completely extended, to the position where the
Home to fixed out: 0156 steps
MTP is completely inserted.
Home to full-dark edge: 1538 steps HOME POSITION always refers to the point at
which the MTP is fully extended, for this reason
Home to rear: 2860 steps
it is always zero.
Measurement mode: FAST Entire Transport path is , for example, 2860
steps
Measurement Offsets:
SUNRISE:
IN: -350000µm
OUT: -525000µm
Spectra/ATC:
FAST
IN:-1 Steps
OUT: -3 Steps
ACCURACY
IN:-1 Steps
OUT: -3 Steps
CENTER
IN:-1 Steps
OUT: -3 Steps
Last ERRORS :
0: ERR:0403: No filter carriage detected
1: ERR:0505: Invalid command sequence
2: ERR:0505: Invalid command sequence
3: ERR:0505: Invalid command sequence
4: ERR:0505: Invalid command sequence
5: ERR:0403: No filter carriage detected
6: ERR:0205: Not expected transport Rear IRQ
7: ERR:0207: Transport inserted 3 steps
8: ERR:020A: Lamp high
9: ERR:020A: Lamp high
Internal Checksum :
00016EC3h - 000000B5h - 000008F1h - 000002F5h Internal
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-5
9. Sunrise Service Tool Kits for Sunrise RC and Options
Duration Test
The following is an example of the results given by a Duration test:
9-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-7
9. Sunrise Service Tool Kits for Sunrise RC and Options
3. Switch on the instrument and the new Magellan 4.When the screen is dark the download
will be downloaded automatically. progress is finished.
5. Switch off the instrument and remove the 6.Insert the memory card and witch on the
Flashcard. instrument.
7. Calibrate the display by clicking the arrow. The 8.The start screen appears.
arrow will then reappear in a different position.
Click again on this arrow. Continue until the start Touch screen is ready to start testing.
screen appears.
9-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
9.8.1 General
The barcode upgrade kit consists of the following:
Installation
Manual
Reader
Package
Sunset
Software
Adjustment
Microplate
Screws
Laser
Scanner
Barcode
Board
Mirror
Option Flex Cable
The Sunrise barcode reader supports only the following barcode types:
• CODE 39
• Interleaved 2 of 5
• UPC A, UPC E0, UPC E1
• EAN8, EAN 13
• Codabar
• CODE 128
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-9
9. Sunrise Service Tool Kits for Sunrise RC and Options
9.8.2 Installation
The barcode option unit is installed using the following procedure:
Carefully pull the instrument cover backwards, approx 1-2 cm and lift upwards
to remove.
9-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
NOTE:
ENSURE THAT THE CATCH AND COVER ARE NOT DAMAGED.
POWER
Lamp
Compartment
Cartridge / Filter Compartment Lid Lid
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-11
9. Sunrise Service Tool Kits for Sunrise RC and Options
M 3x8
Plastite
M 3x12
Plastite
Install the barcode board and the barcode laser by attaching the screws and
connecting the flex cable of the laser to the barcode board at ST1.
M3x8
Plastite
ST1
B 2,2 x 6
9-12 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
Clasp
Open the brown clasp of the connector on the BCR-Board –connect the cable
and close the clasp again.
ST3
ST10
NOTE:
BY INSTRUMENTS WITH INSTALLED OPTIONS THE FLEX CABLE IS
ALREADY INSTALLED.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-13
9. Sunrise Service Tool Kits for Sunrise RC and Options
Laserbeam Label
1404981xx
Laser Radiation
Label 1404967xx
Laser Label
1404968xx
Laser Label
1404968xx
9-14 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-15
9. Sunrise Service Tool Kits for Sunrise RC and Options
In the User configuration dialog box under Options, select Barcode and click OK.
Insert all defined filter slides step by step for automatic calibration.
Disconnect the instrument from the Sunset software and close the Sunset
software.
Install the reader package CD and install the X-read plus software
Start the RdrOle4 Server program.
9-16 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
Instrument / Connect
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-17
9. Sunrise Service Tool Kits for Sunrise RC and Options
Enter the single command “TR0” in the “command” field and press the send
button – plate transport out
TR0
Answer: +
Place the adjustment microplate on the plate support, so that the reticule is on the
right side (mirror side).
9-18 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
Enter the single command “TR1” in the “command” field and click the send button
– Plate transport in
TR1
Answer: +
Enter the single command “DB IBUS SEND B V” in the “command” field and
press the send button – get barcode Firmware version
DB IBUS SEND B V
Answer: +V 1.xx
BCR
Enter the single command “DB IBUS SEND B R” in the “command” field and
press the send button – Reset Barcode module
DB IBUS SEND B R
Answer: +
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-19
9. Sunrise Service Tool Kits for Sunrise RC and Options
Enter the single command “DB MCU POS 24” in the “command” field and press
the send button – Plate transport in BCR position
DB MCU POS 24
Answer: -
Enter the single command “DB IBUS SEND B S” in the “command” field and
press the send button – start barcode scanning
NOTE:
ENTER A BARCODE RESET (DB IBUS SEND B R) STEP IN THE COMMAND
FIELD BEFORE ENTERING THE BARCODE SCANNING COMMAND.
DB IBUS SEND B S
Answer: +
The barcode scanner will then be switched on for five seconds. During this time,
adjust the laser beam with the mirror screw (see picture below), so that the laser
beam is aligned with the reticule on the adjustment microplate.
The barcode scanner may have to be switched on more than once in order to
align the laser properly.
9-20 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
9. Sunrise Service Tool Kits for Sunrise RC and Options
Enter the single command “TR0” in the “command” field and press the send
button – remove the Plate transport.
Turn the microplate plate, so that the barcode is on the right side.
Enter the single command “TR1” in the “command” field and press the send
button – drive in the Plate transport.
Enter the single command “DB MCU POS 24” in the “command” field and press
the send button – Plate transport to BCR position.
Enter the single command “DB IBUS SEND B R” in the “command” field and
press the send button – Reset barcode module.
Enter the single command “DB IBUS SEND B S” in the “command” field and
press the send button – start barcode scanning.
Enter the single command “DB IBUS SEND B G” in the “command” field and
press the send button – get barcode Data.
DB IBUS SEND B G
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9-21
9. Sunrise Service Tool Kits for Sunrise RC and Options
9-22 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
10 Maintenance
10. Maintenance
Caution
The disinfection procedure should be performed by authorized trained
personnel, in a well-ventilated room, wearing disposable gloves, protective
glasses and clothing.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 10-1
10 Maintenance
Name:...................................................................................................................
Firm: .....................................................................................................................
Address: ...............................................................................................................
.............................................................................................................................
.............................................................................................................................
Country:................................................................................................................
Signature:.............................................................................................................
10-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
10 Maintenance
10.5.1 Daily
No daily maintenance is required.
10.5.2 Weekly
Clean the Touchscreen display (when available), cover and the plate transport
with a mild detergent.
Caution
Never use Acetone, as it will damage the covers.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 10-3
10 Maintenance
Relative Humidity
Event 10% 40% 55%
10-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
10 Maintenance
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 10-5
11. Performance Testing / Quality Control
11.1 Introduction
This chapter gives the instructions on how to obtain the best performance and
accuracy from this instrument.
Also included are instructions on how to easily check the performance of the
instrument.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 11-1
11 Performance Testing / Quality Control
11-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
11. Performance Testing / Quality Control
11.3.1 QC PAC 2
QC PAC 2 provides an automated check of reader performance including
accuracy, linearity, precision and alignment. It also detects damaged or
mislabelled filters. For more information, see QC PAC 2 manual.
Caution
Only use QC PAC 2 for SUNRISE instruments. The former version of the QC
PAC 2 (for SPECTRA instruments) is not fully compatible with the
instrument.
Acceptable Microplate
1 2 3 4 5 6 7 8 9 10 11 12
A 0.042 0.039 0.045 0.041 0.039 0.037 0.043 0.043 0.040 0.039 0.043 0.041 A
B 0.042 0.042 0.040 0.040 0.042 0.040 0.045 0.043 0.039 0.038 0.043 0.039 B
C 0.043 0.040 0.040 0.043 0.041 0.041 0.042 0.042 0.041 0.046 0.043 0.039 C
D 0.043 0.043 0.047 0.038 0.039 0.040 0.040 0.041 0.042 0.039 0.039 0.049 D
E 0.041 0.044 0.046 0.043 0.039 0.040 0.040 0.042 0.043 0.041 0.045 0.044 E
F 0.046 0.042 0.041 0.043 0.042 0.052 0.043 0.047 0.045 0.044 0.041 0.040 F
G 0.041 0.043 0.041 0.040 0.042 0.042 0.041 0.040 0.043 0.043 0.041 0.041 G
H 0.042 0.040 0.040 0.044 0.045 0.039 0.041 0.046 0.045 0.044 0.040 0.045 H
1 2 3 4 5 6 7 8 9 10 11 12
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 11-3
11 Performance Testing / Quality Control
Unacceptable Microplate
1 2 3 4 5 6 7 8 9 10 11 12
A 0.104 0.105 0.110 0.134 0.136 0.168 0.147 0.140 0.163 0.154 0.167 0.188 A
B 0.119 0.107 0.110 0.151 0.133 0.168 0.153 0.138 0.165 0.167 0.167 0.178 B
C 0.111 0.117 0.121 0.141 0.146 0.136 0.156 0.150 0.158 0.173 0.170 0.182 C
D 0.112 0.101 0.113 0.153 0.146 0.127 0.139 0.143 0.152 0.165 0.163 0.170 D
E 0.105 0.109 0.114 0.135 0.120 0.131 0.142 0.138 0.143 0.161 0.163 0.163 E
F 0.096 0.106 0.110 0.138 0.132 0.128 0.128 0.149 0.158 0.155 0.161 0.172 F
G 0.097 0.110 0.112 0.125 0.133 0.125 0.120 0.132 0.145 0.155 0.168 0.156 G
H 0.095 0.090 0.096 0.144 0.129 0.124 0.129 0.139 0.131 0.150 0.151 0.161 H
1 2 3 4 5 6 7 8 9 10 11 12
Me a surem ent Po sitio ns Me a surem ent Po sitio ns Me a surem ent Po sitio n Me a surem ent Po sitio n
for Fa st a nd Ac c ura te for Fa st a nd Ac c ura te for Ce ntre Mo d e for Ce ntre Mo d e
Mo d es Mo d es
11-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
11. Performance Testing / Quality Control
Agglutination Method
Open SUNRISE Instrument Setting software to set the SPECTRA Mode. For
agglutination measurements an external software package such as Software
2000 must be used.
After the microplate has been measured, select a well and zoom it so that it is
printed in large scale.
Normal Liquid Liquid with High Meniscus
R C C R R C C R
100% 100% 100% 100%
0% 0% 0% 0%
1 10 20 30 40 1 10 20 30 40
Measurement Measurement
If the printout has less than sixteen measurement points in the middle of the well,
that are at the same level, use the center measurement.
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 11-5
11 Performance Testing / Quality Control
Manual Method
If the instrument is not able to perform Agglutination measurements, measure the
microplate five times.
Then rotate the microplate by 180 ° and then measure the microplate five times.
For a number of wells, calculate the average optical density value from all the
measurements for these wells.
Compare the average value against the highest and lowest measured values.
Example
Measured Values
0.945, 0.956, 0.937, 0.926, 0.971, 0.936, 0.961, 0.939, 0.942, 0.938
Average = 0.945, Highest = 0.971, Lowest = 0.926
Tolerances = (0.945 ± 0.5 % and ± 0.005)
Highest value within tolerance = 0.955
Lowest value within tolerance = 0.935
Ensure that the values are within the allowed tolerances, if not use the Center
measurement mode.
Repeat the procedure using the Center measurement, to ensure that the
measured values are now within the required tolerances.
11-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
11. Performance Testing / Quality Control
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 11-7
11 Performance Testing / Quality Control
11-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
11. Performance Testing / Quality Control
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 11-9
12. Trouble Shooting
12.1 Introduction
error number error text possible defective part that see chapter
causes the problem
0x0203 "Not expected Optical switch within transport box is 3.5.5. Plate calibration
transport FixedOut broken, plate calibration values got
IRQ" lost
0x0204 "Not expected Optical switch within transport box is 3.5.5. Plate calibration
transport Rear broken, plate calibration values got
IRQ" lost
0x0205 "Transport lost %s Optical switch within transport box is 3.5.5. Plate calibration
steps" broken, plate calibration values got 3.5.8 Duration test
lost
0x0206 "Transport inserted Optical switch within transport box is 3.5.5. Plate calibration
%s steps" broken, plate calibration values got 3.5.8 Duration test
lost
0x0207 "Transport can't Optical switch within transport box is 3.5.5. Plate calibration
find HOME broken, plate calibration values got
position" lost
0x0208 "Transport can't Optical switch within transport box is 3.5.5. Plate calibration
find REAR broken, plate calibration values got
position" lost
0x0209 "Lamp high" too much light to perform a reliable 3.5.4 Mirror and lamp
measurement adjustment
0x020A "Lamp low" too less light to perform a reliable 3.5.4 Mirror and lamp
measurement adjustment
0x020B "Timeout waiting check power board and lamp 3.5.4 Mirror and lamp
for lamp on" adjustment
0x020C "Timeout waiting Problem with the transport system 6. Mechanical system
for measurement see transport box, optical switches transport box replacement
finished" broken
0x020D "Timeout waiting Problem with the transport system 6. Mechanical system
for transport see transport box, optical switches transport box replacement
finished" broken
0x020E "Not expected Problem with the transport system 6. Mechanical system
transport IRQ %d see transport box transport box replacement
during calibration"
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 12-1
12. Troubleshooting
error number error text possible defective part that see chapter
causes the problem
0x020F "Transport couldn't Problem with the transport system 6. Mechanical system
find full dark edge see transport box transport box replacement
during calibration"
0x0210 "Transport lost %d Problem with the rear sensor of the 6. Mechanical system
steps during transport box transport box replacement
calibration "
0x0211 "Transport check settings of Measurement 3.4.5 Define Measurement
frequency too low: Mode Mode
%d < %d"
0x0212 "Transport check settings of Measurement 3.4.5 Define Measurement
frequency too high: Mode Mode
%d > %d"
0x0301 "Writing to flash Problem with the main board 7.4.1 replacing the
failed" Mainboard
0x0302 "Verifing flash Problem with the main board 7.4.1 replacing the
failed" Mainboard
0x0303 "Erasing flash chip Problem with the main board 7.4.1 replacing the
failed" Mainboard
0x0304 "Erasing flash Problem with the main board 7.4.1 replacing the
block failed" Mainboard
0x0305 "Unexpected flash Problem with the main board 7.4.1 replacing the
device" Mainboard
0x0306 "Flash is busy" Problem with the main board 7.4.1 replacing the
Mainboard
0x0401 "Filter is busy" Filterslide can not be inserted during
measurement
12-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
12. Trouble Shooting
error number error text possible defective part that see chapter
causes the problem
0x0408 "Filter carriage not Check filterslide coding slits 5.5.3 Filter Coding for
defined => Sunrise Filter Carriages
Type:%d,
Number:%d"
0x0409 "No Gradient Filter Check filterslide coding slits 5.5.3 Filter Coding for
detected" Sunrise Filter Carriages
0x0601 "Wrong checksum Check filter settings 3.5.7 User configuration
in configuration
block 1"
0x0602 "Wrong checksum Check filter settings 3.5.7 User configuration
in all configuration
blocks !"
0x0603 "Writing of all Check filter settings 3.5.7 User configuration
configuration perform setup all see 3.5.2
blocks failed !"
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 12-3
12. Troubleshooting
Error message Alignment: Check optical system, replace 3.5.2. perform setup all
appears on the transport box
printout cover Overflow: failed
page
Error message Filter Verification: Check if filter settings corresponds 3.5.2. perform setup all
appears on the with the hardware setting of the filter
printout cover e.g. slide or exchange filter
page Filter
A B C ... H
620 Failed
12-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
13. Parts for Sunrise
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 13-1
13. Parts for Sunrise
13-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
13. Parts for Sunrise
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 13-3
13. Parts for Sunrise
13-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
13. Parts for Sunrise
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 13-5
13. Parts for Sunrise
13-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
13. Parts for Sunrise
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 13-7
13. Parts for Sunrise
13-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002
13. Parts for Sunrise
February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 13-9
13. Parts for Sunrise
13-10 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002