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Human populations have been shown to dif- from escaped West African slaves and Carib
fer in the magnitude of metric fluctuating den- Indians. Phenotypically the Indian component
tal asymmetry (Bailit et al., '70). Although the is not prominent and admixture estimates sug-
determinants of this asymmetry remain un- gest that about 80% of the Carib genome is
clear (DiBennardo and Bailit, '78; O'Rourke West African in origin (Crawford '79). Creoles
and Crawford, '791, environmental stress has are for the most part hybrid African and Euro-
been suggested as a contributing factor (Di- pean. During recent times, little intermarriage
Bennardo and Bailit, '78). Varying degrees has been reported, despite the fact that t h e two
of discrete dental trait differences have been groups are highly mobile (Gonzales '69).
observed a m o n g populations ( B a u m e a n d
Crawford, '78) however there has been little MATERIALS AND METHODS
investigation of populational differences in re- Approximately 700 dental casts were col-
gard to frequencies of discrete dental trait lected by anthropologic field teams in t h e four
asymmetry. The purpose of this study is to test Mexican communities between 1969 and 1972.
whether t h e asymmetry of discrete dental In Belize 282 dental casts were collected a t two
traits is observed differentially between popu- locations, Dangriga and Punta Gorda, during
lations and ethnic groups. the summer of 1976. For all samples, alginate
This study is comprised of four related Mexi- impressions were made directly from dentition
can communities and two related ethnic groups of individuals who were receiving medical care
from Belize. The four Mexican Indian groups a t public health clinics. The casts were then
a r e the historically and genetically related poured in dental stone. Nine maxillary and
communities of C u a n a l a n , S a n Pablo del seven mandibular discrete traits were scored
Monte, Tlaxcala City and Saltillo. Cuanalan on each cast.
and Saltillo were originally settled in the 17th The discrete dental traits scored and the scor-
century by transplanted Tlaxcaltecans. S a n ing techniques used in this study are summa-
Pablo is a n Indian population from the Valley rized in Table 1. Only permanent teeth were
of Tlaxcala, while the City of Tlaxcala is a utilized, and the age and sex of each subject
mixed (Mestizo)community from the same val- were recorded. Not all traits could be scored on
ley. The trihybrid populations of Mexico a r e all casts, either because of agenesis or excessive
primarily Indian with some Spanish and little wear. Inter-observer error is of no significance
African ancestry (Crawford et al., '79). in this study since all casts were scored by one
Caribs and Creoles, the two Belizean ethnic
groups, are hybrid populations. Caribs stem Ileceived Octnher :10. IYiK, accepted Mxy :11. 1979
0002-9483/80/5203-0:~15$~1
70 (1 1980 ALAN K. LISS, INC 315
316 R.M. BAUME AND M.H. CKAWFOKD
Maxillary teeth
Shovelling I' 0.99 (228) 0.96 (29) 0.93 (72) 0.97 ! 104) 0.88 (86) 0.99 (73)
Shovelling I' 0.98 (2281 0.75 (301 0.76 (72) 0.90 (1031 0.83 (84) 0.89 (66)
Rotation I1 0.91 (2311 1.00 (30) 0.73 (72) 0.96 (107) 0.77 (95) 0.79 (74)
Rotation I' 0.40 ( 2 3 2 ) 0.71 (31) 0.66 (721 0.62 (107) 0.57 (84) 0.69 (71)
Canine ridges 0.86 (214) 1.00 (311 0.78 (65) 0.87 (95) 0.74 (77) 0.81 (72)
Cusp number Mi 0.67 (188) 0.60 (251 0.70 (58) 0.65 (91) 0.88 (71) 0.93 t64J
Cusp number M2 0.88 (63) 0.52 (81 0.80 (12) 0.85 (481 0.82 (36) 0.83 (31)
Carahelli's cusp M1 0.79 (1861 0.93 (25) 0.81 158) 0.82 (91) 0.85 (73) 0.87 (67)
Carabelli's cusp M' 1.00 (58) _ _ __ 0.53 (44) _ _* 0.76 (321
Mandibular teeth
Rotation I , 0.53 1238) 0.85 1311 0.43 (72) 0.35 (1071 0.69 (85) 0.70 (741
Rotation I, 0.44 (238) 0.60 (311 0.47 (70) 0.39 (106) 0.45 (821 0.50 (74)
Canine ridges 0.83 1225) 0.69 1251 0.75 150) 0.70 (78) 0.66 (74) 0.70 (60)
Cusp pattern M , 0.98 ! 1491 0.97 ! 151 0.68 (47) 0.79 I671 0.90 t70) 0.86 (49)
Cusp pattern MZ 0.91 (531 _ _ 0.73 (9) 0.88 (43) 0.93 135) 0.88 (22)
Protostylid M I 0.85 ! 155) _ _ 0.70 (57) 1.00 t75) 1.00 173) 0.87 (49)
Protostylid M, _ _ x; __ _ _* 1.00 (451 1.00 (41) - -*
Sample sizes in parentheses.
xCoefficient data cannot h e computed
Table 3. Percentage asymmetry for each trait for euch group, average asymmetry,
and differences when Belize is compared to each Mexican sample.
~ ~ ~~ ~~
Maxillary teeth
Shovelling I' 1.75 3.45 4.16 2.88 1.16 9.58
Shovelling I' 10.97 10.00 20.83 19.41 13.09 10.60
Rotation I' 2.60 0 11.11 11.21 10.53 8.10
Rotation I' 14.67 6.45 8.33 10.28 14.28 8.45
Canine ridges 9.35 0 15.38 12.63 14.29 19.44
Cusp number M' 15.43 12.00 7.00 10.99 5.63 6.25
Cusp number M' 11.11 12.50 17.00 10.42 11.11 9.67
Carabelli's cusp M' 20.97 16.00 17.24 28.57 17.81 20.89
Carabelli's cusp M' 0 - - 4 54 - 6.25
Mandibular teeth 5
Rotation I , 9.24 6.45 19.44 15.88 9.41 9.45
Rotation I2 16.39 9.68 16.25 20.56 19.51 18.33 e
Canine ridges 10.67 12.00 10.00 24.35 22.97 31.66
Cusp pattern M, 1.34 6.67 6.38 7.46 11.43 2.04
Cusp pattern M, 5.66 - 0 2.33 5.71 4.55
Protostylid M, 0.65 1.75 0 0 2.04
Protostylid M I - - - 0 0 -
Mean asymmetry 8.72 7.93 11.06 11.35 10.46 11.16
Differences between percentages
Caribs and other groups 0.1844 0.4834* 0.4972- 0.4545 0.4846*
Total Belize and Mexico = 0.4998
'P 0 05 - 0 4750
DENTAL. TRAIT ASYMMETRY 319
females, both sexes were scored as a single dental trait asymmetry for two reasons. First,
group. since there is an abundance of evidence that
Finally the relationship between tooth size discrete dental traits are at least in part under
and discrete trait asymmetry was tested utiliz- genetic control (Goodman, '65; Alvesalo et al.,
ing the point biserial correlation coefficient '75) there is reason to suspect that discrete trait
method. The results are shown in Tables 4 and asymmetry may also be under genetic control.
5. As Gruneberg ('65) has shown, nonmetric den-
These tables indicate that for molar pattern tal traits in mice are basically under genetic
and number, and for shovelled incisors there is, influence and can be expressed as continuous
for the most part, a negative correlation be- variables. The presence of a trait, then, may
tween tooth size and asymmetry. However in result when a genetically determined threshold
all but one case the correlation is not signifi- point on the continuum is exceeded. If genetic
cant. For Carabelli's cusp and protostylid this influence is strong and the same genes control
negative correlation is viewed across the board. both sides of the dental arch, then there should
Although no pattern is indicated for canine be a high frequency of bilateral expression,
ridges, rotation of incisors shows a clear posi- while greater environmental influence should
tive correlation in each sample. Most of these result in greater asymmetry (Bailit et al., '70).
are not significant, however, most values are In this study, Table 3 shows that asymmetry in
close to being significant. the Mexican and Belizean teeth is relatively
It is interesting to note that for the largest low, indicating some genetic influences. Sec-
sample, the Caribs, the asymmetry of most ond, in a study by O'Rourke and Baume ('77)on
traits is significantly correlated with tooth size. the same dental casts, the Belizean teeth,
Therefore, the lack of significance in the which show less asymmetry in this study, were
smaller groups may in fact be an artifact of found to be significantly larger than the teeth
small sample size. of the Mexican groups, which show more
Positive correlations of tooth size and asym- asymmetry. Therefore, since the genetic role in
metry may be explained by the nature of the determining tooth size is well documented
trait itself. For example, tooth rotation may (Goodman,'651, a negative correlation between
result from crowding of teeth in the dental arch; tooth size and asymmetry should not be dis-
since larger teeth are more apt to be crowded counted. Moreover the point biserial coefficient
and therefore mechanically rotated, it is ex- ofcorrelation test indicated that for the discrete
pected that large teeth are positively correlated traits-shovelling of incisors, Carabelli's cusp,
with rotation. protostylid, and molar cusp number and
pattern-such a negative correlation does in
DISCUSSION fact exist.
The high degree of significance of association Yet, environmental influences on the denti-
between left and right quadrants in this study tion cannot be ruled out either, since they can
suggests that the genetic factors influencing result in a variety of interactions with geno-
discrete traits may be the same for both sides of types, culminating in a wide range of pheno-
the dental arch, as has been suggested by Green types. For example, the expression of Carabel-
('67).However, the expressivity of the trait may li's cusp has been shown to increase when
vary so that its ultimate expression is influ- flouride is ingested during tooth formation
enced either by local environmental conditions (Cox et al., '61). Moreover, Alvesalo et al. ('75)
within the jaw andlor by intrauterine devel- found a low heritability for Carabelli's cusp. In
opmental factors. The significant populational addition, Bailit et al. ('70),have indicated that
differences between Belize, which has mostly fluctuating dental asymmetry is greatest in
African ancestry and the Mexican samples populations experiencing the greatest envi-
which are predominantly Indian and Mestizo, ronmental stress.
suggest that there are different genetic and CONCLUSION
environmental components contributing to the
final phenotypic asymmetry for each of the Studies of metric dental asymmetry have
groups. The problem in identifying the causes concentrated on environmental and devel-
of dental trait asymmetry is that information opmental stress as a causal agent. The denti-
concerning interactions of environmental and tion begins to form and develop during the pre-
genetic mechanisms is lacking. Genetic factors natal period. However, DiBennardo and Bailit
cannot be completely ruled out in regard t o ('78)found the evidence for a positive relation-
w
t
o
Table 4 . Paint hrserial correlation coefficrent(males) 0
~ - ~ _ _ _ _ _ ~
ship between individual asymmetry and pre- micrcdifferentiation of two transplanted Tlaxcaltecan
natal stress in humans to be equivocal. In this populations. in: The Tlaxcaltecans, M.H. Crawford, ed.
Publications in Anthropology, No. 7, University of Kan-
study the gross physical and ecologic envi- sas, Lawrence.
ronments differ greatly between Belize and Crawford, M.H. (1979)Population structure of Black Caribs.
Mexico, as well as between each of the Mexican In: Population Biology of Black Caribs, M.H. Crawford, ed.
groups, yet mean dental asymmetry clusters Publications in Anthropology University of Kansas,
Lawrence (manuscript)
together in Belize and Mexico. This suggests Dahlberg, A.A. (1963) Analysis of American Indian Denti-
that asymmetry may in fact be influenced more tion: Dental Anthropology MacMillan, New York.
by prenatal or intrauterine environmental Dahlherg, A.A. (1956) Materials for the establishment of
stress, than by ecological considerations. In standards for the classification of tooth characters, attri-
butes and techniques in morphological studies of the denti-
addition, this stress may be greater in Mexico tion. Zoller Laboratory of Dental Anthropology. Univer-
than in Belize. sity of Chicago Press, Chicago.
DiBennardo, R., and H.L. Bailit (1978) Stress and dental
ACKNOWLEDGMENTS asymmetry in a population of Japanese children Am. J.
Phys. Anthropol., 48:8%94.
Dennis O’Rourke and Seishi W. Oka col- Gonzales, N.L. ( 1969) Black Carib Household Structure.
lected some of the dental casts used in this University of Washington Press, Seattle.
study. This research was supported in part by Goodman, H.O. (1965) Genetic parameters of dentofacial
National Institute of Dental Research grant development J. Dent. Res. 44.176184.
Green, D.L. < 1967) Genetics dentition taxonomy. University
DE04115-01 and Career Development Award of Wyoming h b l . , 33:9%168.
KO4 DE028-0 1. Gruneberg, H. (1965) Genes and genotypes affecting the
teeth of the mouse J. Embryol. Exp. Morph., 14: 137-156.
LITERATURE CITED Morris, D.H. (1965) The Anthropological Utility of Dental
Morphology. Unpublished Ph.D. dissertation, University
Alvesalo, L., M. Nutila, and P. Protin !1975) The cusp of of Arizona, Tuscon.
Carabelli. Acta Odontol. Scand., 33:191-197.
ORourke, D.H., and R.M. Baume 11977) Dental variability
Bailit, H.L., P.L. Workman, J.F. Niswander, and C.J. Mac- among the Black Caribs of Belize. Paper presented a t the
Clean (1970)Dental asymmetry as an indicator of genetic American Anthropological Association Meetings-
and environmental stress in human populations. Hum. Houston, Texas, December 1977.
Biol., 42:62&638. O’Rourke, D.H., and M.H. Crawford !1979)Fluctuating den-
Baume, R.M., and M.H. Crawford (1978) Discrete Dental tal asymmetry in four populations of related genetic back-
Traits in Four Tlaxcaltecan Mexican Populations Am. J. ground Am. J. Phys. Anthropol. (In press).
Phys. Anthropol., 49: 35 1-360.
Cox, G.J., S.B. Finn, and D.B. Ast (1961) Effects of flouride Sokal, R.L., and F.J. Rohlf (1969)Biometry. W.H. Freeman
ingestion on the size of the cusp of Carabelli during tooth and Co., San Francisco.
formation J. Dent. Res., 40:39.%395. Walker, H.M., and J. Lev (1953)Statistical Inference. Henry
Crawford, M.H., R. Lisker, and B.P. Briceno (1976) Genetic Holt and Co., New York.