Biomedical Engineering

BIOCHEMISTRY
1st Sem 2020

PA - BIOQUÍMICA - 2020SEM01

HOMEWORK ENZYME KINETICS

Name: Jorge Andrés Gutiérrez Guimi

Find KI for inhibitor 2.

Table 1. Inverse of concentration and speed of the reactions in the presence of inhibitor.

Inverso de Inverso de velocidad Inverso de velocidad inverso de velocidad con

concentración sin inhibidor con inhibidor 1 inhibidor 2

1 0,083333333 0,23255814 0,181818182

0,5 0,05 0,125 0,111111111

0,25 0,034482759 0,071428571 0,076923077

0,125 0,028571429 0,047619048 0,0625

0,083333333 0,025 0,038461538 0,055555556

As shown in class, since the Km values for the reactions are the same but the Vmax values are

not, we are facing with a noncompetitive inhibition:

Noncompetitive inhibition
0,2

0,15

0,1
1/V

0,05

0
-0,6 -0,4 -0,2 0 0,2 0,4 0,6 0,8 1 1,2
-0,05
1/[S]
y = 0,0632x + 0,0195 y = 0,1372x + 0,0438
Inverso velocidad sin inhibidor inverso de velocidad con inhibidor 2
Lineal (Inverso velocidad sin inhibidor) Lineal (inverso de velocidad con inhibidor 2)

Figure 1. Lineweaver-Burk graph of the reaction without inhibitor and in the presence of inhibitor 2.

1
 Biomedical Engineering
BIOCHEMISTRY
1st Sem 2020

To find KI in this case we use:

𝑉𝑚𝑎𝑥
𝑉𝑚𝑎𝑥𝑎𝑝𝑝 = (1)
[𝐼]
1+
𝐾𝐼
Data (obtained from class):
𝑉𝑚𝑎𝑥𝑎𝑝𝑝 = 25, 𝑉𝑚𝑎𝑥 = 50, [𝐼] = 5 𝑚𝑀
Solving:
[𝐼] ∙ 𝑉𝑚𝑎𝑥𝑎𝑝𝑝 5 ∙ 25
𝐾𝐼 = = = 5 𝑚𝑀
𝑉𝑚𝑎𝑥 − 𝑉𝑚𝑎𝑥𝑎𝑝𝑝 50 − 25

So, 5 mM is the KI for the second inhibitor.