RD-50 User's Manual

USER’S MANUAL
OF
RD-50 BIO-CHEMISTRY
ANALYSER
Esteemed customer,
Thank you for acquiring our instrument. Its features, we are sure, will
make it a valued instrument for your laboratory. Although it can be
operated in a logical and simple way through its menu options, we
recommend you to read this manual carefully. It will help you for
performing the installation and the maintenance correctly, and will
allow you to get the maximum benefit from its many possibilities.
1|Page 1
Contents
1 INTRODUCTION ........................................................................................................................................ 4
2 UNPACKING AND INSTALLATION........................................................................................................... 5
2.1 UNPACKING .....................................................................................................................................................5
2.2 LIST OF CONTENTS ..........................................................................................................................................5
2.3 IDENTIFICATION OF THE COMPONENTS .......................................................................................................5
2.4 INSTALLATION .................................................................................................................................................6
2.4.1 LOCATION ................................................................................................................................................6
2.4.2 INSERTING PRINTER PAPER ROLL ...........................................................................................................6
2.5 CONNECTION TO POWER SUPPLY AND STARTING ......................................................................................6
2.5.1 QUALITY OF THE ELECTRICAL SUPPLY ..................................................................................................6
2.5.2 CHECKING THE SUPPLY VOLTAGE .........................................................................................................7
2.5.3 CONNECTION TO THE POWER SUPPLY .................................................................................................7
2.6 RE-SHIPMENT ...................................................................................................................................................7
3 BASIC OPERATIONS ................................................................................................................................. 8
4 PROGRAM KEY OPERATIONS ................................................................................................................ 10
4.1 PROGRAMING A TEST................................................................................................................................... 10
4.2 EDITING THE NUMBER OF STANDARDS ..................................................................................................... 14
4.3 VIEW THE RECORD ........................................................................................................................................ 19
4.4 COPYING RECORD ........................................................................................................................................ 20
4.5 DELETING A RECORD ................................................................................................................................... 20
4.6 PROGRAM/ RE-PROGRAM ABSORBANCE ................................................................................................... 21
4.7 EDITING OF PT / APTT TESTS ....................................................................................................................... 22
5 FUNCTION............................................................................................................................................... 25
5.1 USER PROGRAM............................................................................................................................................ 25
5.2 LIPID PROFILE CALCULATOR ....................................................................................................................... 26
5.3 PATIENT RECORD.......................................................................................................................................... 27
5.4 DAYS REPORT ............................................................................................................................................... 28
5.5 SETTING ......................................................................................................................................................... 29
5.5.1 SET TIME & DATE................................................................................................................................... 29
5.5.2 EDIT UNITS ............................................................................................................................................ 30
5.5.3 AIR PURGE VOLUME ............................................................................................................................. 30
5.5.4 DISTILL WATER VOLUME...................................................................................................................... 30
5.5.5 GLOBAL PRINTER STATUS .................................................................................................................... 30
5.5.6 DETAILED PRINITNG ............................................................................................................................. 30
5.5.7 CHANGE PASSWORD ........................................................................................................................... 31
5.5.8 EDIT DISPLAY LINE................................................................................................................................ 31
5.5.9 SERIAL COMMUNICATION ................................................................................................................... 31
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5.5.10 BUZZER .................................................................................................................................................. 32
5.5.11 PHOTOMETER VALIDATION................................................................................................................. 32
5.5.12 SYSTEM DIAGNOSTICS ......................................................................................................................... 32
5.6 CALIBRATION ................................................................................................................................................ 32
5.6.1 FILTER CALIBRATION ............................................................................................................................ 33
5.6.2 PUMP CALIBRATION ............................................................................................................................. 33
5.6.3 FLOW CELL TEMPERATURE CALIBRATION ......................................................................................... 34
5.6.4 EDIT PUMP CONSTANT ........................................................................................................................ 35
5.6.5 EDIT TEMPERATURE SET POINTS ......................................................................................................... 35
5.7 EDIT HOME PAGE.......................................................................................................................................... 35
5.8 SERVICE ......................................................................................................................................................... 35
5.8.1 NUMBER OF FILTERS ............................................................................................................................ 36
5.8.2 EDIT FILTERS.......................................................................................................................................... 36
5.8.3 ADC REFERENCE VOLTAGE .................................................................................................................. 36
5.8.4 DELETE MEMORY .................................................................................................................................. 37
5.9 LAMP SAVER MODE ...................................................................................................................................... 37
6 TEST EXECUTION .................................................................................................................................... 38
6.1 KEYBOARD TEST ........................................................................................................................................... 38
6.2 USER PROGRAMMED TEST .......................................................................................................................... 38
6.3 END POINT EXECUTION ............................................................................................................................... 38
6.4 END POINT EXECUTION ............................................................................................................................... 43
6.5 KINETICS ........................................................................................................................................................ 43
6.6 FIXED TIME (TWO POINT KINETICS) ............................................................................................................ 46
6.7 ABS OD KEY OPERATION ............................................................................................................................. 48
6.8 PT AND APTT................................................................................................................................................. 49
7 QUALITY CONTROL ................................................................................................................................ 52
8 Technical Specifications: ....................................................................................................................... 54
9 ERRORS & WARNINGS ............................................................................................................................ 56
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1 INTRODUCTION
This instrument is intended for general use, but its features are especially appropriate for usage in
clinical laboratories.
Despite the large number and the complexity of tasks that it is capable of performing, its operation is
extraordinarily simple through the menu system and the underlying logic of its interface, so that an
easy-to-assimilate dialogue is established between the user and the instrument.
Besides great reliability in the optics, this instrument offers versatility in the measurement devices:
flow micro-cuvette, common cuvettes or micro-cuvettes, and tubes. The cuvettes are thermo
regulated in a rapid and precise manner by means of the Peltier system, without requiring
connections for circulating water.
Its sophisticated software allows the programming of up to 235 different records with required
parameters that define a set test. It can store data of up to 200 distinct tests. On the other hand,
programming is simple and is facilitated by the existence of commands for “copy” and “move”.
Besides the tests, many other functions of the instrument can be programmed, such as: laboratory
name, new filters, wash mode, contents of the print header, communications, etc.
The RD-50 Chemistry Analyzer is a programmable analyzer, capable of providing

quantitative results for up To 235 different tests in the following Categories:
Available methods are:

● Therapeutic drugs ● Endpoint
● Enzymes ● Fixed time
● Plasma proteins ● Kinetic
● Electrolytes ● Bichromatic
● Substrates ● Absorbance
● Coagulation
The RD-50 Chemistry System analyzer: Some Facts and Features

● Serum ● Use of flow cell or disposable
cuvettes
● Plasma ● Display prompts to easily guide you
● Whole blood (Hemoglobin) through test procedures
● Urine ● Integrated quality control program
Includes printed control chart.
● Upgradeable software via PC
● 35 Standard keyboard tests; and
200 available by Entering test code
number
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2 UNPACKING AND INSTALLATION
This equipment is a precision instrument, so it is necessary to exercise special care in its
installation and positioning.
It is very important to connect the instrument to a good electrical system. It should be as
exclusive as possible, and it is absolutely imperative for it to have an earth connection.
2.1 UNPACKING
On receiving the instrument, check that the packing is in perfect condition and the seals are
intact. Open the box and take out its contents with care. Read this chapter carefully before installing
the instrument. Do not throw the packing material away. In case of re-shipment, use it again to
guarantee good transport.
2.2 LIST OF CONTENTS
The set of items that the user will find on unpacking the box
◙ RD-50 - Instrument:
• Plastic Cuvette 5 Pcs.
• Power Cord 1 Pc.
• Dust Cover 1 Pc.
• Waste Bottle 1 Pc.
• Waste Bottle Stand 1 Pc.
• Printer Rolls (1x2) 2 Pcs.
• Fuses 2 Pcs.
• Operating Manual CD 1 Pc
2.3 IDENTIFICATION OF THE COMPONENTS
The Constituent parts of the instrument are identified and numbered as below and the associated
lists.
Front Side
1. LCD Display 5. Printer Paper Roll 9. Sliding Cuvette Section

2. Key board 6. Access Cover
3. Aspiration Tube 7. LED Printer on / off
4. Aspiration Switch 8. LED Paper Out
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Rear Side
1. On/Off Switch 6. Fan
2. Fuse Holder 7. Peristaltic Pump
3. Power cord Socket 8. Pump – In (Vacuum)
4. RS 232 Socket 9. Pump – Out (Pressure)
5. USB Port
2.4 INSTALLATION
In order to ensure an optimal instrument operation, follow the instructions given in this
chapter to install the instrument.
2.4.1 LOCATION
The instrument must be situated in a dry and non-corrosive environment. In addition, it must
not be near a source of electro-magnetic radiation (such as motors, centrifuges, etc.) or a source of
heat, nor directly receive intense lamp or sunlight.
It must be located on a flat and spacious surface, taking care that no object obstructs the
outlet of air from the fan. Keep a distance/gap of at least 15 cm to the nearest wall.
The waste bottle must be placed on the same level or lower than the instrument.
2.4.2 INSERTING PRINTER PAPER ROLL
a) Open the paper access cover by pulling the lever and put a paper roll (13) in its loading,
ensuring that the thermo-sensible face of the paper is facing to the bottom of the lodging.
b) Close the top cover just by pressing it from the top.
2.5 CONNECTION TO POWER SUPPLY AND STARTING

Starting the instrument involves observing some aspects of the electrical supply to which it is
to be connected, as well as carrying out some simple checks.
2.5.1 QUALITY OF THE ELECTRICAL SUPPLY
It is very important to connect the instrument to a good electrical system. It should be as

exclusive as possible, and it is absolutely imperative for it to have an earth connection.
If a malfunctioning of the instrument is noticed (Program crashes, sporadic re-starts, etc.)
check that it is not near centrifuges or machines containing motors or electro-magnets, which can
generate strong electrical noise. In such a case, place the instrument far from such equipments.
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2.5.2 CHECKING THE SUPPLY VOLTAGE
This instrument is designed to work at the following voltages: 220 V AC (Range 90- 270 VAC / 50
Hz)
ATTENTION
Working beyond the range limits will cause the Instrument to function incorrectly
and the instrument may be damaged.
2.5.3 CONNECTION TO THE POWER SUPPLY
Once the voltage selected in the apparatus corresponds to that of the electrical supply, proceed as
follows:
a) Remove the label covering the power socket
b) Check that the switch is in the off (0) position.
c) Connect the power cable, first to the apparatus, then to the electrical supply.
d) Put the switch in the on (1) position.
2.6 RE-SHIPMENT
If it is necessary to re-ship the instrument for any reason, or to make a move involving the use
of any transport vehicle, it is important to use the original packing to ensure that the Instrument does
not suffer any damage. .
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3 BASIC OPERATIONS
When the system is powered on, main/ welcome screen will appear as shown
below.
Wait System Booting ……..
Testing RTC
Warm up time: 180 sec

Please wait.. or Press FUNCTION key to wash
flowcell
Press No to continue…..
If FUNCTION key to be pressed, instrument ask for aspiration of 2 ml distilled water and wash the
flowcell by forward as well as reverse flow done by the pump. And then it will ask for 2 ml washing
solution to clean the flowcell from deposition. Or press ESC to continue.
Testing Optical System…
Aspirate 1 ml of distilled water

Press NO key to skip the optical system check.
Company :- RECKON DIAG.PVT.LTD.

URL :- reckondiagnostics.com
SERIAL No : - 12345
RD-50
Clinical Bio-chemistry Analyzer
Time:04:04:31 Date: 29/01/15
All the keys of the keyboard are active when user is in main/ welcome screen.
8|Page 8
PROGRAM: This key allows the user to program, edit, view, copy and delete a
test Record.
FUNCTION: This key allows the user to view and run the user programmed
tests, and has the provision to set, modify the system
parameters.
QC (Quality Control): Press this key to view the QC Levy-Jennings chart for a
particular test sample, and to set/edit the QC concentration
range for level 1 & level 2.
ABSORBANCE: Press this key to determine the absorbance value of a test sample.
WASH: Press this key to rinse the flow cell.
PRINT ON/OFF: Press this key to switch ON and OFF the printer.
Test keys: These keys allow the user to run the standard test.
Digit keys: These keys allow the user to run the user programmed test.
Arrow Keys: ◄/► ▼/▲ use as a move cursor.
Enter/yes: This key allows user to select any test.
Esc/No: key to abort test and return to main/ main screen.
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4 PROGRAM KEY OPERATIONS
The <PROGRAM> key allows user to:
Program a test.
Edit the parameters in the test record.
View the test record.
Copy record from one location to another.
Delete a particular test record.
Program/ re-program Absorbance function.
4.1 PROGRAMING A TEST
The following are the steps/procedure to program a test:
PROGRAM To program/edit a key board test, press the corresponding

Press a Test key to Edit: test key or to program/edit the user program, enter the location.
OR Press ◄ key to erase a digit.
Enter a User Program Press <Enter/YES> key to select the location.
Location [0-199] : _ Press <ESC/NO> key to abort and return to main /welcome
screen.
ENT / ESC
NOTE:
Enter user program location from 0 to 199.
On pressing <Enter/YES> from the previous menu, the following screen appears.
PROGRAM [XXX] SCREEN WITH ENTERED LOCATION
XXX: Indicates the entered location number of a test.
PROGRM [ XXX] Enter the test name using the alpha

Test name: _ numeric keys.
<continue> Press ◄ key to erase a digit/character.
Press <Enter/YES> key to select the test
Select continue for next name.
window Select continue using ▼key and press
ENT/ESC ▼▲ <Enter/YES>.
Press <ESC/NO> key to abort and
return
to previous screen.
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When a key board test is pressed, option for entering test name screen will not appear and
directly the following screen appears.
KEYBOARD TEST:GLUC Press ▼/▲ keys to navigate.

1 VIEW Select EDIT and press <Enter/YES> key.
2 EDIT Press <ESC/NO> key to abort and return to previous
3 COPY screen.
4 DELETE
ENT/ESC ▼▲
When a key board test is not pressed, if user enters his location number to program a test,
then following screen appears.
PROGRAM [XXX]: ABCD Press ▼/▲ keys to navigate.

1 VIEW Select EDIT and press <Enter/YES> key.
2 EDIT Press <ESC/NO> key to abort and return to previous
3 COPY screen.
4 DELETE
ENT/ESC ▼▲
NOTE:
If the record is being programmed for the first time, the default parameters are
displayed. The following screen appears when the EDIT option is selected from the previous
menu.
DISPLAY PAGE 1:-
ABCD : EDIT
Method [END POINT]
Sub Method WITH STANDARD
Wavelength 1 [505] nm
Wavelength 2 [NIL] nm
Units [mg/dL]
Temperature [37] C
Num of Standards [NA]
Standard Conc.* 100.00 mg/dL
Factor [NA]
Reaction Slope [Increasing]
*conc: ENT/ESC ◄/► ▼/▲ concentration.

Press ► key to view/edit next page.
Press ◄ key view/edit previous page.
TYPES OF ALGORITHMS:
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MAIN METHODS SUB-METHODS
◙ End point:-
- Standard.
- Factor.
- Sample blank standard.
- Sample blank factor.
◙ Kinetics:- - Standard
- Factor.
◙ Fixed time:-
- Standard.
- Factor.
◙ Differential end point:-
- Standard.
- Factor.
◙ Multi Standard:-
- End point
- Kinetic
- Fixed time
SELECTING METHOD AND SUB METHOD:
Press <Enter/YES>, the following screen appears and select main method.
1. END POINT Press ▼/▲ to navigate.

2. KINETICS Press <Enter/YES> to select the method.
3. FIXED TIME Press <ESC/NO> key to abort and return
4. MULTI STANDARD to previous screen.
5. DIFFERENTIAL
Once main method is selected, another window with sub methods appears as shown below.
EXAMPLE:
The table below shows the sub methods for end point method.
1 WITH STANDARD Press ▼/▲ to navigate.

2 WITH FACTOR Press <Enter/YES> to select the method.
3 WITH SMP/BLK STD Press <ESC/NO> key to abort and return to
4 WITH SMP/BLK FACT previous screen.
SELECTING WAVE LENGTH:
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340 nm Press ▼/▲ to navigate.
405 nm Press <Enter/YES>, to select wavelength /
450 nm Filter.
505 nm Press <ESC/NO> key to abort and return to
546 nm Previous screen.
578 nm
630 nm
700 nm
NIL
NOTE:
Bi-chromatic measurement is applicable only for Endpoint, Differential endpoint and
Multi Standard endpoint tests. Select NIL for Wavelenght2 to disable bi-chromatic
measurement.
SELECTING UNITS:
mg/dL Press ▼/▲ to navigate.

g/dL Press <Enter/YES>, to select units.
U/L
mU/L
mg/mL
ug/mL
mEq/L
Ug/dL
mMol
SELECTING TEMPRETURE:
25 Press ▼/▲ to navigate.

30 Press <Enter/YES>, to select temperature.
37
RT
*RT: Room Temperature.
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4.2 EDITING THE NUMBER OF STANDARDS
This is applicable only for Multi standards test method. Enter required number of
standards (Valid range: 3 - 10). Press <Enter/YES> to select the value.
NOTE:
If value is greater than 10, error message shown below will appear and again you have to
enter a valid number.
Please Enter value between (3-10):
EXAMPLE:
If the number of standards is 5, screen will appear and you can enter standard
concentration value.
1. 100.00 Press ▼/▲ to navigate.

2. 200.00 Press <Enter/YES>, to edit and store the values.
3. 300.00 Press ◄ key to erase a digit
4. 400.00 Press <ESC/NO> key to retain old value.
5. 500.00
EDITING OF STANDARD CONCENTRATION:
Press <Enter/YES>, to edit standard concentration value.

Press ◄ key to erase a digit
Press <Enter/YES>, to store the values.
Press <ESC/NO> key to retain old value.
EDITING OF FACTOR:
Press <Enter/YES>, to edit factor value.

Press ◄ key to erase a digit.
Press <Enter/YES>, to store the values.
SELECTING REACTION SLOPE:
Press <Enter/YES> to select reaction slope.
1 Increasing. Press ▼/▲ to navigate

2 Decreasing. Press <Enter/YES>, to select slope.
14 | P a g e 14
DISPLAY PAGE 2:
ABCD :EDIT
No .Decimals [1]
Delay Time 5 sec
Read Time [NA]
Delta Time [NA]
Reagent volume 1 500 µL
Reagent volume 2 0 µL
Sample volume 50 µL
Aspirate volume 490 µL
Linearity 500.00 mg/dL
Blank ABS Limit 0.000
ENT ESC ◄/► ▼/▲

Press ◄ key to view/edit previous page.
SELECTING NUMBER OF DECIMAL VALUE:
0 Press <Enter/YES>
1 Press ▼/▲ to navigate
2 Press <Enter/YES>, to store decimal value.
EDITING OF DELAY TIME:

Press <Enter/YES>
Enter required delay time in seconds.
Press <Enter/YES>, to store delay value.
EDITING OF READ TIME:

Press <Enter/YES>.
Enter required read time in seconds.
Press <Enter/YES>, to store read value.
EDITING OF DELTA TIME:

Press <Enter/YES>
Enter required delta time in seconds.
Press <Enter/YES>, to store delta value.
15 | P a g e 15
EDITING OF REAGENT VOLUME 1:
Press <Enter/YES>
Enter required volume.
Press <Enter/YES>, to store reagent volume value.
EDITING OF REAGENT VOLUME 2:
Press <Enter/YES>
Press <Enter/YES>, to store reagent volume value.
EDITING OF SAMPLE VOLUME:
Press <Enter/YES>
Press <Enter/YES>, to store sample volume value.
EDITING OF ASPIRATE VOLUME:
Press <Enter/YES>.
Press <Enter/YES> to store aspirates volume value.

NOTE: Valid aspirate volume range is: 250 to 2000 µL

During test run phase when LCD ask for “Aspirate sample “ and WASH key is pressed then, the
instrument will aspirate the volume as set in the aspiration volume and not 1000ul of default
volume. Then pressing ENTER key will allow the operator to take the reading by bypassing the
ASP key if the ASP key is not working. ( Wash and then ENTER key will work as same as ASP key)
EDITING OF LINEARITY:
Press <Enter/YES>
Enter required Linearity.
16 | P a g e 16
Press <Enter/YES>, to store value.
EDITING OF STARTING ABSORBANCE:
Press <Enter/YES>
Enter required Absorbance value.
NOTE: Valid starting absorbance range is: 0.0 to 3.5.
DISPLAY PAGE 3:
ABCD:EDIT
Normal Max 10.00 mg/dL
Normal Min 5.00 mg/dL
External Incub Time 5 Minutes
Printer on/off [Printer ON]
QC Level-1 Target 0.00 mg/dl
QC Level-1 Max 1.00 mg/dl
QC Level-1 Min 0.00 mg/dl
ENT / ESC ◄/►▼/▲

Press ◄ key to view/edit previous page.
Press <Enter/YES>
Enter required Value of controls
Press <ESC/NO> key to retain old value
Same process follow for editing level-1,2 &3 control values
NOTE: OD max (Optical Density) and OD min are applicable for only Kinetics, Fixed time and
multi standards with kinetics and fixed time.
EDITING OF NORMAL MAXIMUM VALUE:
Press <Enter/YES>
Enter required Value.
Press <ESC/NO> key to retain old value
17 | P a g e 17
EDITING OF NORMAL MINIMUM VALUE:
Press <Enter/YES>
Enter required value.
NOTE: Always Normal maximum should be greater than Normal minimum.
EDITING OF EXTERNAL INCUBATION TIME:
Press <Enter/YES>
Enter required incubation time.
SELECTING OF PRINTER ON/OFF:
1 Printer ON Press <Enter/YES>

2. Printer OFF Press ▼/▲ to navigate
Press <Enter/YES>, to select on/ off.
DISPLAY PAGE 4:
ABCD:EDIT
Inventory 999
QC Lot number 0000
Cali. valid Days 00
ENT / ESC ◄/► ▼/▲
EDITING OF INVENTORY:
Press <Enter/YES>
Enter required inventory value.
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Once the required parameters are edited for a test/record, press ESC KEY to save the
record.
Depending upon the main method and sub method of a test, some of the parameters are
not applicable and NA is displayed against it, which indicates that that particular parameter is
not applicable for that particular test.
Press <Enter/YES>
Enter required value of control
Enter the lot number of control.
When edit the lot no of control following screen will
appear
Changing the lot No. will delete the

existing QC data.
Press YES to continue….
Enter the validity period of celebration.(only in

EP,FT)
EDIT THE PARAMETERS IN THE RECORD:
Once the test or record is programmed, user can re-program or edit the same record for
performing execution of test. User can edit only the required parameters and need not edit all
the parameters, and perform the test.
4.3 VIEW THE RECORD
In VIEW mode, user can only view the parameters of the test record, No editing of parameter
will take place in this view mode.
PROGRAM [XXX]:ABCD
1 VIEW
2 EDIT
3 COPY
4 DELETE
19 | P a g e 19
Press ▼/▲ to navigate inside screen.
Select VIEW and press <Enter/YES>.
Press <ESC/NO> key to abort and return to previous
screen.
ENT/ ESC ▼▲
4.4 COPYING RECORD
Only an edited/ saved record can be copied to another user program location.
EXAMPLE:
Suppose if record number 55 (user program record) is to be copied to another user
program location for e.g. 162.
Select COPY using ▼/▲ keys and press <Enter/YES> key to select the location. After
pressing <Enter/YES> key, system will ask for password. Enter the valid password and message
as shown below will be displayed.
ENTER THE DESTINATION RECORD

NUMBER (0-199) : _
Once the above message appears, enter the number 162, after entering the location
press <Enter/YES> key. The record gets copied to that location. Valid destination locations is (0-
199).
The below shown message will be displayed, once record is copied.
RECORD COPIED!
NOTE: Key board tests can be copied to any location from 0 to 199.
4.5 DELETING A RECORD
To delete a record, Select DELETE using ▼/▲ keys and press <Enter/YES> key, system
will ask for password, as shown
ENTER THE PASSWORD : _****
PASSWORD for deleting record 3048
Enter the valid password and press <Enter/YES>, message as shown below will be
displayed.
Are You Sure (Yes / No)?
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If you press <ESC/NO> key, then record does not get deleted, record gets deleted only
when a <Enter/YES> key is pressed. After deleting a record, delete successful will be displayed
as shown below.
Delete Successful.
Once the above message is displayed, the record gets deleted from the list and contents
of that record will be lost.
4.6 PROGRAM/ RE-PROGRAM ABSORBANCE
ABSORBANCE TEST EDITING:
Absorbance test parameters can be edited, after pressing program key from main screen, the
following screen appears
PROGRAM
Press a Test key to Edit: Press Absorbance key, to edit absorbance
OR parameters.
Enter a User Program :
Location [0-199]: _
ENT/ESC
After pressing Absorbance key, screen with following parameters will appear.
Absorbance
WL 1 [505] nm
WL 2 [NIL]
Temperature [37] c
Asp Volume 490 µL
Printer on/off [Printer on]
Delay 00 sec
No. of prints 999
ENT/ESC ▼/▲
Editing of each parameter is as described below.
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EDITING OF WAVE LENGTH:
340 nm
405 nm
450 nm Press ▼/▲ to navigate.
505 nm Press <Enter/YES>, to select wavelength1/filter.
546 nm After selecting wavelength and press <Enter/YES>.
578 nm
630 nm
700 nm
NIL
SELECTING TEMPRATURE:
25 Press ▼/▲ to navigate.

30 Press <Enter/YES>, to select Temperature.
37 Select Temperature and press <Enter/YES>.
RT
EDITING ASPIRATE VOLUME:

Press <Enter/YES>
Press <Enter/YES> key to store aspirate volume
value.
NOTE: Valid aspirate volume range is: 250 to 2000 µL.
SELECTING PRNTER ON/OFF:
1 Printer ON Press <Enter/YES>

2. Printer OFF Press ▼/▲ to navigate
Press <Enter/YES>, to select on/ off.
record.
4.7 EDITING OF PT / APTT TESTS
PT TEST EDITING:
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Prothrombin Time (PT) Test parameters can be edited, after pressing PT test key from program
screen
PROGRAM
Press a Test key to Edit: Press PT test key, to edit parameters.
OR
Location [0-199] : _
ENT/ESC
After pressing PT key, screen with following parameters
will appear.
PT
Wave Length [405] nm
Temperature [37] ‘c
Plasma Inc Time 120 sec
ISI Value 1.95
PT Normal 12 sec
Plasma Volume 100 uL
PT Reag Volume 200 uL
Delta ABS 0.0048
ENT/ESC ▼/▲
NOTE: Wavelength and Temperature are fixed for PT test; please do not edit these two
parameters. If trying to edit these two parameters will have no effect and they do not get
edited.
Edit the other parameters, if required. Editing of each parameter is as same as

editing of a keyboard test or programming a new test.
record.
APTT TEST EDITING:
Activated Partial Thromboplastin Time (APTT) Test parameters can be edited, after pressing
APTT test key from program screen.
PROGRAM
Press a Test key to Edit: Press APTT test key, to edit parameters.
OR
Location [0-199]: _
ENT/ESC
After pressing APTT key, screen with following parameters will appear.
23 | P a g e 23
APTT
Wave Length [405] nm
Temperature [37] ‘c
Plasma + Aptt Inc 180 sec
Time 100 uL
CaCl2 Reagent 26 sec
APTT Normal 100 uL
Plasma Volume 100 uL
APTT Reag Volume 0.0048
Delta ABS
ENT/ESC
▼/▲
NOTE: Wavelength and Temperature are fixed for APTT test; please do not edit these
two parameters. If trying to edit these two parameters will have no effect and they do not get
edited.
Edit the other parameters, if required. Editing of each parameter is as same as

editing of a keyboard test or programming a new test.
record.
24 | P a g e 24
5 FUNCTION
The <FUNCTION> key allows user to:
View and run the user programmed tests.

Set and edit the system parameters, such as
i) Time and date
ii) Pump calibration
iii) Filter calibration
iv) Serial communication
v) View / Print Patient record
vi) Temperature
vii) Filters addition
viii) Password
ix) Display
5.1 USER PROGRAM

When <FUNCTION> key is pressed from main/welcome screen, following screen will be
displayed.

Press <ENTER/YES> key to select.
1. USER PROGRAM Press <ESC/NO> key to abort and return to
2. LIPID PROFILE CALCULATOR previous screen
3. PATIENT RECORD
4. DAYS REPORT
5. SETTINGS
6. CALIBRATION
7. EDIT HOME PAGE
8. SERVICE
Function screen-1
VIEW/RUN USER PROGRAMMED TESTS:-
Select USER PROGRAM to view/run/print user programmed location.
0: 10: 20: 30: Press ◄/► keys to switch between

1: 11: 21: 31: pages.
2: 12: 22: 32:
. . . . When user is in the above screen, user can
. . . . i) Print the records with its location
. . . . number and test names, which are already stored.
9: 19: 29: 39: To print the stored records, just press PRINT KEY,
all the records with record number location along
Page 1/5 Enter a Record No: _ ◄/► with its name will be printed.
There are totally five pages and each page consists
of 40 records. Test names or record names will be displayed against the record number if the
records are edited/ stored.
25 | P a g e 25
EXAMPLE:
If you store test name as ABCD in location 55 while editing of a record, then ABCD
will be stored in location 55, which is available in page2. In status bar, you can enter a record
number to run the test. Enter 55 and press <Enter/YES> key. You will watch a window with
following options.
1. VIEW PARAMETERS
2. RUN
3. PRINT PARAMETERS
4. EDIT PARAMETERS
5. DELETE RECORD
Press 1, to view parameters of that record.

Press 2, to run / execute the record.
Press 3, to print parameters of that record.
Press 4, to edit parameters of program (password for editing program-3048)
Press 5, to delete program (password for deleting program- 1234)
In status bar, if you enter a record number 56 or other record numbers which are not edited and
saved, then you will see a window with following message.
Record is Empty!
NOTE:
In status bar, do not enter a record number which is greater than 199. If you enter 200 or
above, system will display warning message as shown below.
Invalid Record Number!
5.2 LIPID PROFILE CALCULATOR

LIPID PROFILE CALCULATIOR can be done by entering in to this mode.

Select LIPID PROFILE CALCULATOR and
1. USER PROGRAM press <Enter/Yes>
2. LIPID PROFILE CALCULATOR Press <ESC/NO> key to abort and return
3. PATIENT RECORD to previous screen
4. DAYS REPORT
5. SETTINGS
6. CALIBRATION
7. EDIT HOME PAGE
8. SERVICE
Select and press enter on LIPID PROFILE CALCULATOR to go into into following screen.
LIPID PROFILE CALCULATOR
26 | P a g e 26
P.ID 1 VLDL Press ▼/▲ to navigate inside
DATE YY/MM/DD LDL screen.
TRIG T.LIPID Press <Enter/YES> after selecting
CHOL LDL/HDL any one.
HDL CHOL/HDL
YES / NO / PRINT ▼/▲
Feed the value of TRIG, CHOL, HDL and the parameters of other screen will be calculated
automatically.
Press PROG key to print all record.
5.3 PATIENT RECORD
1. USER PROGRAM
2. LIPID PROFILE CALCULATOR
3. PATIENT RECORD
4. DAYS REPORT
5. SETTINGS
6. CALIBRATION
7. EDIT HOME PAGE
8. SERVICE
Allows user to view and print the patient’s record stored in memory.
1. To view patient records:
On selecting Patient record in Utilities-1 menu, the following window pop’s up on screen.
Patient record screen.1

On selecting “View record” the following screen is displayed.
Sl no PID Date ABS CONC
Press ▼/▲ to navigate within the page.
1 456 26/05/07 1.03 50.06 Press ◄/► to view previous/next page.
2 457 26/05/07 1.03 50.06 Press <ENTER/YES> key to display detailed record
3 458 26/05/07 1.03 50.06
4 459 26/05/07 1.03 50.06 (Screen-3)
Press <PROG > key to print record.
Press <WASH> key to delete all the patient
Page 1/10 records.
Press <ESC/NO> key to return to Patient record
screen-1.
Sl no PID Date ABS CONC
1 456 26/05/07 1.03 50.06

2 457 26/05/07 1.03 50.06 Patient record screen.2
3 458 26/05/07 1.03 50.06
4 459 26/05/07 1.03 50.06 To view the detailed record press <ENTER/YES> on the
particular record. A window pop’s up as show in screen-3.
Page 1/10
Press <PRINT ON/OFF> key to print the record.
27 | P a g e 27
Press <ESC/NO> key to return to screen-2.
Pid: 456
Date: 26/05/07
Time: 10:23:06 AM
Factor: 240.06
ABS: 1.03
CON: 50.06
Patient record screen.3

2. To print patient records:
Select “Print Record” from patient record screen-1.
Enter the patient record number.

Press <ENTER/YES> key to start printing*.
Press <ESC/NO> key to abort printing and return
Enter the patient ID : _ to screen-1.
5.4 DAYS REPORT
1. USER PROGRAM
3. PATIENT RECORD
4. DAYS REPORT
5. SETTINGS
6. CALIBRATION
7. EDIT HOME PAGE
8. SERVICE
DAYS REPORT
DATE TEST
YY/MM/DD
Enter date, Patient ID & particular test name for
P.ID generating test reports on particular date.
ALL
ALL
TEST REC.NO In case of generating all the test record of
ALL
particular patient select all & press enter on
GENERATE REPORT Generate Report.
Through this process you get all the test record of
particular patient of that day.
REC. NO
YES / NO / PRINT ▼/▲ In case of generating report from 0-199 user
defined location, please enter the particular
location number.
28 | P a g e 28
5.5 SETTING
1. USER PROGRAM
3. PATIENT RECORD
4. DAYS REPORT
5. SETTINGS
6. CALIBRATION
7. EDIT HOME PAGE
8. SERVICE
SETTING PASSWORD - 4020
SETTING SETTING
1. SET TIME & DATE 7. CHANGE PASSWORD

2. EDIT UNITS 8. EDIT DISPLAY LINE
3. AIR PURGE VOLUME : 30ul 9. SERIAL COMMUNICATION
4. DISTILL WATER VOLUME : 1000ul 10. BUZZER : ON
5. GLOBAL PRINTER STATUS : ON 11. PHOTOMETER VALIDATION : ENABLE
6. DETAILED PRINTING : (G+R+I) 12. SYSTEM DIAGNOSTICS
PAGE ½ PAGE 2/2

▼/▲◄/► ▼/▲◄/►
5.5.1 SET TIME & DATE
To set time and date, select “SET TIME AND DATE” and press < ENTER/YES KEY>
Press <Enter/YES> to set time and date.

Press <ESC/NO>, to go to previous screen.
After pressing <Enter/YES>, set time, in the screen shown below.

Time And Date
Set Time ( Hr: Min: Sec)::_ Enter hour, minutes and seconds.
Press <ESC/No> key to abort and return to
previous screen
Hr: Hour is in 12 hour mode, enter hour value from 1 to 12, and press <Enter/Yes>
Min: Enter minute value from 00 to 59, and press <Enter/Yes>.
Sec: Enter seconds value from 00 to 59, and press <Enter/Yes>.
Now, use ▼/▲ to select PM or AM mode, and press <Enter/YES>.
Setting Year, Month and Date.
29 | P a g e 29
Time And Date Press <ESC/NO> to set date.
SetTime(Hr:Min:Sec)::xx:xx:xx Press ◄ key to erase a digit
Time: 03 : 45 : 25 PM Press <ESC/NO> to abort and to previous screen.
Set Date (yy/mm/dd) :_

Date: 13 . 03 . 20
yy: Enter year value from 00 to 99, and press <Enter/Yes>.
mm: Enter month value from 1 to 12, and press <Enter/Yes>.
dd : Enter date value from 1 to 31, and press <Enter/Yes>.
Press <ESC/NO> key to abort and return to previous screen.
5.5.2 EDIT UNITS

Not applicable for users
5.5.3 AIR PURGE VOLUME

In between two samples in any test, a small amount of air is aspirated to nullify the carry over effect.
Air purge volume should not be set more than 60 µl.
5.5.4 DISTILL WATER VOLUME

In performing any test, the instrument opts for aspiration of distilled water. That volume can be
edited in this option. It can be edited from 1000 to 9999 µl.
5.5.5 GLOBAL PRINTER STATUS
GLOBAL PRINTER STATUS : ON

OFF
To disable the printer for all operation set the status to OFF.
5.5.6 DETAILED PRINITNG
DETAILED PRINTING: ONLY RESULTS (R)
RESULTS & INTERMEDIATE VALUES (R+I)

GRAPH & ALL ABOVE (G+R+I)
In test, if you want only results, select only results (R).

If you want results and other abs values, select (R+I).
If you want result, all abs values and graph, select (G+R+I).
30 | P a g e 30
5.5.7 CHANGE PASSWORD
In this option, you can change Calibration Password, Settings Password and User Password.
In case the user wants to change any password from the existing password, the user will have to
enter old password and then new password along with conforming new password.
5.5.8 EDIT DISPLAY LINE
EDIT DISPLAY LINE: EDIT PRINT TITLE

EDIT MAIN SCREEN LINE
Edit Print Title:

Select this option and press ENTER.
Write the Laboratory Name or Company Name which you have to print when the instrument starts
EDIT DISPLAY LINE
LINE 1 _
LINE 2
ENT / ESC ▼/▲
Edit Main Screen Line:
Connect the instrument with computer with RS-232 port.

Open hyper terminal in computer.
Select this option and press ENTER.
Again press ENTER.
Write the name which you want to display on the main screen with the keyboard of computer.
The instrument doesn’t work, while you editing this from computer.
5.5.9 SERIAL COMMUNICATION
After selecting this one, following screen appears

Serial Communication Press <Enter/YES> to selectEnable or Disable serial
1. Serial Communication : Enabled communication.
2. Upload Press ▼/▲ to navigate inside screen
3. Download After selecting press <Enter/YES>
ENT/ESC ▼/▲
Upload:
Do not enter in to this mode, system will stop functioning, incase, if you enter in to this
mode
Once again the system has to be restarted.
Download:
31 | P a g e 31
Do not enter in to this mode, system will stop functioning, incase, if you enter in to this
mode
Once again the system has to be restarted.
Press <ESC/NO> key to abort and return to previous screen.
5.5.10 BUZZER
Buzzer : ON
OFF
If you the instrument to be on silent mode set it to OFF.
Please note that buzzer will remain enabled while performing tests.
5.5.11 PHOTOMETER VALIDATION
Photometer Validation: Enable

Disable
5.5.12 SYSTEM DIAGNOSTICS
Serial Communication To test any component of the instrument, you can select
1. Test LCD one of this and check whether it is working properly or not.
2. Test Pump
3. Test Peliter
4. Test Lamp
5. Test Printer
6. Test Filter Motor
Yes/No
▼/▲
5.6 CALIBRATION
Calibration
1. Filter Calibration
2. Pump Calibration
3. Flowcell Temperature Calibration
4. Incubator Temperature Calibration
5. Edit pump Constant
6. Edit temp Set Point
Yes/No
▼/▲ CELEBRATION PASSWORD
– 0204
32 | P a g e 32
5.6.1 FILTER CALIBRATION
Filter Calibration
1. 340 nm = x.xxxx
2. 405 nm = x.xxxx
3. 000 nm = x.xxxx
4. 505 nm = x.xxxx
5. 546 nm = x.xxxx 9TEMP - x.xxxx
6. 578 nm = x.xxxx Filter Offset - xx
7. 630 nm = x.xxxx
8. 000 nm = x.xxxx
Page2/2
Page1/2 ▼/▲◄/►
▼/▲◄/►
To select 505 nm, press 4 and system will calibrate for
that particular filter.
To select Read temperature press right arrow key.
The < indicates that user has selected that particular filter.
Setting Filter Offset:

Go to 2nd page, go down and select 2. Offset.
Enter offset value and press <Enter/YES>, to save offset value.
Press PROG key and get print out of the filter voltages and do the calibration.
After calibration of filters, Press <ESC/NO> key to abort and return to previous screen.
5.6.2 PUMP CALIBRATION
After selecting this one, following screen appears.
Pump Calibration To calibrate pump

Old Value: xxxx Press <Enter/YES>
Proceed with Calibration?( Yes/ No)

500 ul
Pump calibration volume:
1000 ul
2000 ul
After pressing <Enter/YES>, system will ask for aspiration of water.
Pump Calibration Press Aspirate switch.

Old Value: xxxx
Proceed with Calibration?( Yes/

No)
Aspirate 2 ml of water
Aspirate 2 ml of water by pressing aspirate switch and
release the switch after 2ml of water is aspirated.
The calibrated value is displayed as pump calibrated value: xxx x and previous value is stored in old
value: xxxx, as shown in the screen below.
Once calibration is over, the following screen is displayed with values.
33 | P a g e 33
Pump Calibration
Old Value: xxxx
Press <ESC/NO> key to abort and return to
Pump calibrated value: xxxx previous screen.
Press Any Key ...
5.6.3 FLOW CELL TEMPERATURE CALIBRATION
Filter Calibration Filter Calibration

25˚c Calibration 25˚c Calibration
25˚c Temperature Calibration 30˚c Temperature Calibration
ADC Count: 1721 ADC Count: 1721
Set Point Steps Set Point Steps
1750 01 1650 01
Yes/No Yes/No
▼/▲ ▼/▲
Filter Calibration
Press ▲/▼ keys to navigate.
25˚c Calibration
Press <Enter/YES> key to select the temperature.
30˚c Calibration
Press <ESC/NO> key to abort.
37˚c Calibration
37˚c Temperature Calibration
ADC Count: 1721
Set Point Steps
1125 01
Yes/No
▼/▲
Press ▲/▼ keys to increment/decrement the set points.
Press <ENTER/Yes> key to save set point (read note).
Press <ESC/NO> to abort and select the next temperature to calibrate.
Press ► key to select STEPS.
Note: Wait until the ADC COUNT is settled before pressing <ENTER?YES> key to save the set
point
STEP value indicates by how many values the set point has to increment/decrement. Kindly
record the old temperature ADC values before making any changes and use digital
thermometer with accuracy. Temperature calibration should be with flow cell having distilled
water in it.
34 | P a g e 34
5.6.4 EDIT PUMP CONSTANT
Calibration
Old Pump Constant (2000ul) : 5155
New Pump Constant : xxxx
3. Flowcell Temperature Calibration

4. Incubator Temperature Calibration
5. Edit pump Constant
6. Edit temp Set Point
Yes/No
▼/▲
Edit the new pump calibration value, if the original
value is changed.
Note: Calibrated volume should be same if you directly edit the pump constant.
5.6.5 EDIT TEMPERATURE SET POINTS
Edit Temperature Set Points

Set Point ADC Count
25˚c 1750 1740 Edit the temperature set points as well as ADC counts of all
30˚c 1650 1644 the temperature like, 25°C, 30°C and 37°C of bio-chemistry
and 37°C of incubator section.
37˚c 1125 1145 Press <ESC/NO> key AND PRESS ENTER to save set point.
Incu. 37˚c 860 855
Yes/No
▼/▲◄/►
Set Point ADC Count

25˚c Save the1750 1740 ?
edited values : (Yes/No)
30˚c 1650 1644
37˚c 1125 1145

Incu. 37˚c 860 855
Yes/No
▼/▲◄/►
5.7 EDIT HOME PAGE

5.8 SERVICE
Warning : This menu should only be opened by technical person.
When SERVICE is pressed, it will ask for password, 0204 is the password, following screen
will be displayed.
35 | P a g e 35
SERVICE
1. No. of Filters : 8
2. Edit Filters
3. ADC Reference Voltage : 3.0000
4. Delete Memory
Yes/No
▼/▲
5.8.1 NUMBER OF FILTERS

User can select the number of filters to be used, through keyboard input.
5.8.2 EDIT FILTERS
SERVICE
2. Edit Filters Please don’t change this selection.
4. Delete Memory
340 nm
405 nm
450 nm
505 nm
546 nm
578 nm
630 nm
700 nm
Yes/No
▼/▲
5.8.3 ADC REFERENCE VOLTAGE
Please don’t change this value. This is the basic setting of this instrument. Its value should be 3.0000
Not Applicable for users
36 | P a g e 36
5.8.4 DELETE MEMORY
SERVICE
2. Edit Filters
4. Delete Me
1. Direct Tests
2. User Tests
3. Patient Record
4. QC Record
5. Entire Memory
Yes/No
▼/▲
When you select this option, you will get a new window. By that you can delete all memory sections
separately. You can delete direct test records, 0 to 199 user test records, patient records, QC records
and entire memory.
Note: By selecting entire memory, all the data of the instrument will be lost. Don’t use this
option without concerning any technical person from the company.
5.9 LAMP SAVER MODE

The use of dot key as a lamp saver mode:-
While on the home screen press the dot key once, lamp will go dimmer with less voltage then
normal 12 V supply.
With the use of any test key of users test the lamp is automatically glow with full voltage.
The instrument will select the power saver mode automatically after 10 minutes of idle home
page of LCD i.e. VB-Chem . That is standby mode and when the test is selected, the lamp will
glow with full voltage.
37 | P a g e 37
6 TEST EXECUTION
The following are the steps/procedure to run the 35 keyboard tests and 200 user programmed
tests.
6.1 KEYBOARD TEST

The 35 keyboard tests are executed by pressing the corresponding test key on the keyboard
from main/welcome screen, and follow the on-screen instructions.
6.2 USER PROGRAMMED TEST

The user programmed can be executed in two ways,
1. If the programmed location is known. Press the first digit of the location number when you
are in main/ welcome screen. The following screen appears.
Enter the location number.

Enter the location: _
RD-50 Press <Enter/YES> key to select the location.
Bio-Chemistry analyzer Press <ESC/NO> key to abort and return to main/
welcome screen.
On pressing <Enter/YES> from the previous menu, the following screen appears.
Press <1> to view the parameters of a record/ test on

1. View parameters
2. Run
LCD screen.
3. Print parameters RD-50 Press <2> to execute/ run the test.
4.Edit
5.Delete
Bio-Chemistry analyzer Press <3> to print parameters on to the printer.
Press <4> edit parameter of program(password-3048)
Press <5> delete program(password-1234)
Press <ESC/NO> to abort and to return to main/
welcome screen.
2. If the programmed location is unknown.
6.3 END POINT EXECUTION

The following are the screens that appear while running endpoint test method. The
parameters programmed are displayed on the left side of this screen. The bottom section of the
screen or status bar shows the current status of execution.
I. Temperature:
Wait until temperature reaches the set point or press

<ESC/NO> key to proceed (temperature is updated in
background).
II. Distilled Water:
Attaining Temperature 25`C
38 | P a g e 38
Press aspirate switch to aspirate Distilled water.
Press <Enter/YES> key if the distilled water is already
present in flow cell.
Press <PRINT ON/OFF> key to print parameters to
printer (key board tests only).
Press <ESC/NO> key to abort test and return to main/
Aspirate 1ml Distilled water welcome screen.
NOTE: The above three screens are common to differential endpoint test as well. If the
absorbance value of the blank is above 0.000 then it is stored in the memory and
if the value is 0.000 then the instrument will ask for Reagent Blank. If the factor is
already stored in the memory then the instrument will ask for sample directly
and to calibrate the test again with the standard then please PRESS FUNCTION
key.
III. Aspirate sample:

Press aspirate switch to aspirate new sample reagent.
Press <Enter/YES> key if the sample is already
welcome screen.
Press < Function Key> to run the STANDERD, REAG Blk,
Reag blk + Std, Dist water + Reag Blk + Std
Aspirate Sample
(screen 5 )
Press <PROGRAM> key to edit patient-id, patient name
or edit factor value (Screen-7).
Press <QC> key to store concentration value for quality
control (Screen 8).
Press <WASH> key to rinse the flow cell.
Press < ▼> to reverse aspirate sample from flow cell
back to test tube.
Printer will print first line for Hospital name/ laboratory
name and second line for Doctor or City name during
any test is selected and PRINTER is ON in the program
menu.
IV. PRESS FUNCTION KEY :

i . Run Standerd
Aspirate Standerd:
Press aspirate switch to aspirate Standard
Press aspirate switch to aspirate standard reagent
Once run standard option select you can’t ignore it.
Aspirate Standerd
ii . Run Reag BLK + STD

Store factor:
39 | P a g e 39
Press <Enter/YES> to store new FACTOR value
Press <ESC/NO> to retain the previously stored factor.
Press aspirate switch to aspirate new sample reagent
Factor=250.36: Store?
ii . Aspirate blank reagent:

Press aspirate switch to aspirate reagent blank.
Press <Enter/YES> key if the reagent blank is already
welcome screen.
Aspirate reg blank
Aspirate standard:
Press aspirate switch to aspirate standard reagent.

Press <Enter/YES> key if the standard reagent is already
welcome screen.
Aspirate standard
iii .Run Reag BLK + Standard:
Aspirate blank reagent:

welcome screen.
Aspirate reg blank
Aspirate Standerd:
Store factor:
Aspirate Standerd Press <Enter/YES> to store new FACTOR value
40 | P a g e 40
iv. Run D W + Reag BLK + STD:
Aspirate Distilled Water:

Press <Enter/YES> key if the distilled water is already
Press <PRINT ON/OFF> key to print parameters to
printer (key board tests only).
Aspirate 1000 ul Distilled water welcome screen.
Aspirate blank reagent:

welcome screen.
Aspirate reg blank
Aspirate standard:
Press aspirate switch to aspirate standard reagent.

Press <Enter/YES> key if the standard reagent is already
welcome screen.

Aspirate standard
Store factor:
Press <Enter/YES> to store new FACTOR value

NOTE: Aspirate Sample after four FUNCTION key option .
v. Send data to PC:
If SERIAL COMMUNICATION is enabled in the utilities, the following screen appears.
On receiving ‘R’ transmit ‘y’ to receive data.
Waiting for serial command

41 | P a g e 41
Data format: xxxx, yyyy, zzzz.zz, ±a.aaaa
xxxx : patient id.
yyyy : test name.
zzzz.zz : concentration.
±a.aaaa : absorbance.
Status : H – greater than normal range
L – lesser than normal range
N – within the normal range
vi. Editing the patient ID, patient name and factor value:
Press <Program> key while display shows Aspirate

samples.
PID : 89 Press ▲/▼ keys to navigate.
Patient name: Press <Enter/YES> key to edit values.
Factor: 253.36 Press the numeric keys on keyboard to enter values.
Press <Enter/YES> key again to select the edited values.
Press ◄ key to erase contents.
Press <ESC/NO> key to retain the old value during
editing and also to abort editing and return to previous
screen.
The patient name entered will be printed on the paper as well.
vii. Storing concentration value for QC:
Press <QC> key when display shows Aspirate sample.

QC LEVEL 1
QC LEVEL 2 Press <Enter/YES> key to store concentration value for
QC LEVEL 3 QC.
SAMPLE Select “SAMPLE” to run sample in non-QC mode.
Press <ESC/NO> key to abort and return to previous
Aspirate Sample screen.
After the sample test is completed, the concentration is displayed in the highlighted as shown
in below screen.
CONC:
Aspirate Sample
If the concentration value to be stored is not in the range as specified in QC edit, the following
screen pops up.
Press <Enter/YES> key to store value.

Press <ESC/NO> key to abort.
Current value: 98.36
Max: 103.65
Min: 101.36
Store <Yes/No>?
42 | P a g e 42
NOTE: **The following keys can be pressed during any aspirate phase, applicable while
running all the five test methods.
< ASPIRATE SWITCH >: To aspirate new reagent in to flow call.

OR < WASH > & <ENTER> if ASP key fails or not working
< ENTER/YES >: If the reagent is already present in flow cell.
< WASH >: To rinse the flow cell. Aspirate volume is as programmed.
< PRINT ON/OFF >: To switch on/ off the printer.
< ▼ >: To reveres aspirate the reagent from flow cell back to test tube.
< QC >: To store concentration for QC (only during sample Phase and key board tests).
< PROGRAM >: To edit the patient id number, patient name and the factor value (only during
sample phase).
< ESC/NO >: To abort from the current state and return to previous mode.
<FUNC> : To run Distil water, reagent blank and standard.
6.4 END POINT EXECUTION
The procedure to follow while running the Differential endpoint is similar to that of Endpoint.
The only differences are described in the following two screens.
I. The following screen appears after the factor has been calculated. Enter the number of
samples (1 to 10 samples) for which you need to find concentration.
Use numeric key to enter values.

Press <Enter/YES> key to proceed.
welcome screen.
Enter no... of Sample: _
VB-Chem has the provision of storing the sample absorbance temporarily, so that the user
need not run the sample test again in case of power failure.
Press <Enter/YES> key to aspirate new sample reagent.

Press <ESC/NO> key to proceed with the previously
stored sample absorbance value.
Run new sample <Yes/No>?
To store the concentration for quality control, press <QC> key during the sample phase, and
follow the instruction as discussed in Endpoint section.
6.5 KINETICS
DP-235 has the following features while running the Kinetics/Fixed time* tests.
43 | P a g e 43
1. On Line plotting graph for every second.
2. Graph editing.
On line plotting graph

During the Standard and Sample phase, the change in the absorbance
values with respect to time is displayed on LCD screen as the test is running.
Graph Editing
Here the user has the provision to select the absorbance value according to his
needs and the concentration value is calculated accordingly.
NOTE: * Graph editing feature is applicable only for Kinetics test method and only during
sample phase.
The procedure to run the Kinetics is as follows. The parameters programmed are displayed on
the left side of the screen. The bottom portion of the screen/status bar shows the current status
of execution.
I. Temperature
Wait until temperature reaches the set point or

press <ESC/NO> key to proceed (temperature is
updated in background).
II. Distilled water

Press <Enter/YES> key if the distilled water is
already present in flow cell.
Press <PRINT ON/OFF> key to print parameters
to the printer (key board tests only).
Press <ESC/NO> key to abort test and return to
Aspirate 1ml Distilled water
main/ welcome screen.
III. Aspirate sample
Press aspirate switch to aspirate new sample

reagent.
Press <Enter/YES> key if the sample reagent is
Aspirate Sample
44 | P a g e 44
Press <PROGRAM> key to edit patient-id, patient
name and factor value.
Press <QC>key to store concentration value for
the Quality control.
IV.
The following screen is displayed on pressing <Enter/YES> key or aspirate switch, from the
previous screen. As the test is running, the graph of the change in absorbance value with time
and also the absorbance value and time is displayed in this screen.
During the delay time phase, press <ESC/NO>

key to skip delay time.
.NOTE: The instrument will show the following

ABS: 1.23 after the delay time.
SEC: 80
1) Hyperactive ? If the sample is too high and
Estimated Concentration :32.4
dilution is required .
2) Initial ABS? If the reagent is detoriated and the
abs is not within limit as per the parameter
Absorbance Limit of parameter settings of LCD
screen 2.
V. After the read time is elapsed during the sample phase the following screen is displayed.
Press <Enter/YES> key to edit the graph.

Press <ESC/NO> key to proceed without editing
the graph.
ABS: 1.23
VI. The following screen appears when the user presses the <Enter/YES> key from the
SEC: 80
Edit Graph
previous screen. Here<Yes/No>?
the user has the provision to select the range of absorbance values by
varying the start and end time.
Press ▲ to select right indicator.

Press ▼ to select left indicator.
Press ◄/► to decrement /increment time.
ABS: 1.23 Press <Enter/YES> key to select new time range.
SEC: 80 Press <ESC/NO> key to select the preset time
range.
VII. Printer option:
To print the initial Absorbance of the reagent , delta values, standard deviation and the
GRAPH of Absorbance verses time seconds on the paper,
Press PRINT key for printout or ESC

key
45 | P a g e 45
After the sample test is completed the concentration is displayed in the highlighted, as
shown in screen below.
CONC:
6.6 FIXED TIME (TWO POINT KINETICS)
The procedure to run the fixed time is as follows. The parameters programmed are displayed on
the left side of the screen. The bottom portion of the screen/status bar shows the current status
of execution.
I. Temperature:-
Wait until temperature reaches the set point or

press <ESC/NO> key to proceed (temperature is
updated in background).
II. Distilled water:-

Aspirate 1000 ul Distilled water
III. Calibrate factor:-
Press <FUNC> key to celebrate program

1.Run Distil water
2.Run Distil water + Standard
Press 1 , to run distil water

Press 2 , to run Distil water and standard
46 | P a g e 46
. Distilled water:-

Aspirate 1000 ul Distilled water
Aspirate Standerd:
Aspirate Standerd
V. The following screen is displayed on pressing <Enter/YES> key or aspirate switch from the
previous screen. As the test is running the graph of the change in absorbance value with time

key to skip delay.
ABS: 1.23
SEC: 80
Standard phase
VI. Slope factor:-

Press <Enter/YES> to store new FACTOR value.
Press <ESC/NO> to retain the previously stored
FACTOR.
VII. Aspirate sample:-
Press aspirate switch to aspirate new sample

reagent.
Press <Enter/YES> key, if the sample reagent is
Aspirate Sample
Press <PROGRAM> key to edit patient-id, patient
name and factor value.
Press <QC>key to store concentration value for
the
Quality control.
47 | P a g e 47
VIII.
The following screen is displayed on pressing <Enter/YES> key or aspirate switch from the
previous screen. As the test is running, the graph of the change in absorbance value with time

key to skip delay.
ABS: 1.23
SEC: 80
IX. Send data to PC:

If SERIAL COMMUNICATION is enabled in the utilities, the following screen appears.
On receiving ‘R’ transmit ‘y’ to receive data.
Waiting for serial command

Data format: xxxx, yyyy, zzzz.zz, ±a.aaaa
xxxx : patient id.
yyyy : test name.
zzzz.zz : concentration.
±a.aaaa : absorbance.
Status : H – greater than normal range
L – lesser than normal range
N – within the normal range
After the sample test is completed the concentration is displayed in the highlighted, as shown
in below screen.
CONC:
6.7 ABS OD KEY OPERATION
To measure the absorbance, press <Absorbance> key from main/ welcome

screen.
The parameters programmed are displayed on the left side of the screen. The bottom
section of the screen/status bar shows the current status of execution.
48 | P a g e 48
I. Temperature:-

background).
II. Distilled water:-

Press <Enter/YES> key, if the distilled water is already
welcome screen.
Press <PRINT ON/OFF> key to print parameters to printer.
Aspirate 1000 ul Distilled water Press <ESC/NO> key to abort test and return to main/
welcome screen.
III. Aspirate blank reagent:-

welcome screen.
Aspirate reagent blank
IV. Aspirate sample:-

Press aspirate switch to aspirate new sample reagent.
welcome screen.
ABS:
Aspirate Sample
During the sample phase the absorbance value is displayed in the highlighted
section as shown.
6.8 PT AND APTT
To measure Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT),
Press <PT> or <APTT> on key board and follow the on screen instructions.
49 | P a g e 49
When press PT/APTT key instrument will guide to remove flowcell.
Following screen will appear
Remove flowcell press < YES>

Key
Instrument will giving option as under
Select require test by arrow key

PT TEST
APTT TEST
After selection of test instrument will give option about patient ID,name,normal value
and ISI value.
P.ID ________
Name ________
Normal ________
ISI ________
I. Temperature:-

background).
Attaining Temperature `C
II. Plasma sample:-
Insert plasma sample cuvette and press <Enter/YES> key

to start incubation.
welcome screen.
Press < PROGRAM > key to edit ISI value (PT test only)
Insert plasma sample cuvette
III. Incubation
Press < ▲ > key to increase incubation time or press < ▼

> key to decrement incubation time.
Press < ESC/No > key skip incubation.
Sec: 180
Pre-incubation time
50 | P a g e 50
Add reagent and press < ENTER/Yes > key if test is
programmed as MANUAL mode or just add reagent if test is
programmed as AUTO mode.
Press < ESC/No > key to abort test.
Add 200 ul PT reagent
The graph of the absorbance w.r.t time is shown on

LCD.
The absorbance and time elapse is displayed on LCD.
SEC: 80
ABS: 1.23
Clot detected A message window pops up when the clot has been
detected.
SEC: 14 Results are displayed and printed.
ABS: 1.23
After detecting clote press < ENTER/YES > than result will as under screen shown
Temp
37
ABS:
1.55
INR :
Clotting Time : 14.3
51 | P a g e 51
7 QUALITY CONTROL
To view the Levy-Jennings chart or to edit/set the QC minimum and maximum values Press
<QC> key from the main/welcome screen and follow the on screen instructions.
1 .
Press a test key spline

Press < ESC/NO > key to abort and return to
main/welcome screen.
Press a test key or
Enter record no-
no-0 to 199
2 .
QC - ABCD
Sample
1. View level -1 0 Press <Enter/YES> key to select.
2. View level -2 0
3. View level – 3 0
Press <ESC/NO> key to abort and return to
previous screen.
ABCD mean test name
3 .
1.View Data
2. View chart
This screen is overlap on screen 2 , select option by navigating arrow key up/down.
Select 1st option to view data about record of tests record.
Select 2nd option to view Levy-Jennings chart.
4 .
When the number of sample stored is less than five, the following screen appears.
Press <Enter/YES> or <ESC/NO> key to continue.

In sufficient sample
Sample
1. View normal 2
2. View abnormal 9
3. Edit normal
4. Edit abnormal
NOTE: A minimum of five samples has to be stored to view the Levy-Jennings chart.
52 | P a g e 52
5 . Levy – Jennings chart:-
Press <Print ON/OFF> key to print chart onto

printer.
Press ‘1’ to delete the QC values.
Press <ESC/NO> key to return to previous
screen.
Press ‘1’ to delete QC values
To edit/set the QC minimum and maximum values of the normal level 1 and abnormal level 2
samples, select ‘Edit normal’ or ‘Edit abnormal’ from screen2.

Press <Enter/YES> key to edit.
Max: 123.3 Sample Use numeric keys to enter values.
Min:
1. View 101.36
normal 2
2. View abnormal 9 Press <ESC/NO> key to retain the previous value.
3. Edit normal Press <ESC/NO> key to save and return to
4. Edit abnormal
previous screen.
53 | P a g e 53
8 Technical Specifications:
METHODS OF CALCULATIONS
Absorbance
End Point with standard / factor
Kinetics
Fixed time
Differential
Coagulation
Multipoint up to 10 standards
OPTICAL SYSTEM
Principle : Absorption spectrometry
Range of measurement : From – 0.1 to 3.0A
Nominal spectral range : Automatic via filter wheel.
Filters : 340, 405, 450, 505, 546, 578, 630 and
700nm interference type
Bandwidth : 10 nm (± 2nm)
Light source : Halogen lamp 12V / 20W OR 6V/10W
Zero : Automatic
Settings : Monochromatic and Bichromatic readings.
Detector : Schottkey type Photodiode – Silicon
THERMOSTAT SYSTEM
Peltier regulated 25°, 30° & 37°C programmed / RT
Accuracy : ± 0.1°C
SCREEN AND KEYBOARD

Screen : Graphic LCD 240 x 128 pixels
Back Light : Blue LED Back lit
Keyboard : USB Type Optional
PROGRAMMING
Table of tests : up to 235
Table of units : up to 9 units
Quality control storage of the last 30 results
Memory : (1000 Test Results)
QUALITY CONTROL
3 Controls per test - Normal & Abnormal
Levy-Jennings charts & Westgard's rules
COAGULATION :
INR / ISI Value % / Seconds
Auto / Manual start
Parameters : PT / APTT
Filter : 405 nm
INTERFACE
RS - 232 For PC Connection
54 | P a g e 54
CUVETTES
Flow cell of 18 ul OR 32ul
Removable cuvettes : Macro, Semi-micro Micro
Plastic or Glass cuvettes
PRINTER
Type : Fast Thermal Graphic
Printing speed : 50 mm/sec
Dots per line : 384 dots/line
Paper : Thermosensitive - 57 mm wide, Easy Loading
SUCTION SYSTEM
Continuous flow system with peristaltic pump.
Sipping volume can be programmed from 200ul to 1.0 ml.
POWER / DIMENSIONS
Voltage : 110-220 V ( Universal Input)
Frequency : 50 - 60 Hz
Maximum power : <110W
Temperature : 10° to 40° C
Maximum relative humidity:80%
Dimensions : 45 cm x 41 cm x 17 cm
55 | P a g e 55
9 ERRORS & WARNINGS
-: ERRORS & WARNINGS:-
SYSTEM ERRORS:
Error/Warning Cause Solution

1. If battery is weak replace with
RTC NOT RUNNIG / SET TIME 1. Dry Cell 3.3V Battery weak. new battery.
AND DATE / CHECK BATTERY 2. No battery 2. Set time and date.
CHECK FILTER WHEEL MOTOR Sensor failed or Filter wheel Call service
has been jammed.
TEST RUN TIME ERROR:
The following errors and warning is common to all the test methods.

UNKNOWN SUB-METHOD In correct sub-method Reprogram the test.
1. Pump Tighten Flow cell

CHECK FOR AIR BUBBLE 2. In let Tubing to flow cell is Or call service for assistance
loose
CRITICAL INITIAL ABSORBANCE Absorbance is greater than the Check reagent age.
programmed absorbance value.
WRONG SLOPE! The absorbance of the sample is 1. Check reagent

not according to the slope 2. Check program slope.
programmed.
INVALID FACTOR When Abs of Blank and Abs of Check standard and reagent.
Std are same. Div by zero will
occur
INVENTORY Order reagent.
SERIAL COMMUNICATION Serial cable disconnected/ In Check serial cable connection.

FAILED correct protocol. And baud rate:115200,8,-N-1
Concentration is greater than Check sample and dilute if
LINEARITY ERROR the programmed Linearity required.
value.
56 | P a g e 56
Concentration is not in range as
QC NOT IN RANGE set. Check QC min and QC max
value in QC edit menu.
Errors and warning for Kinetics test method.
INVALID DELTA TIME In correct delta time. Edit delta time in test record
Reagent bad or flowcell Clean the flowcell or change

DEVIATION ERROR contaminated the reagent, Dilute the sample
Sample may be to high and run again
Sample may be to high Dilute the sample and run
SUBSTRATE DEPLETED again
Errors and warning for Multi-standards time test method.

In correct delta value.
DELTA ERROR Edit delta time in test record
The absorbance of the sample is not Change the parameters in the

SYSTEM INCONSISTENT according to the slope programmed. program.
Absorbance of the test sample is not Dilute the sample and run
ABS NOT IN RANGE with in the minimum and maximum again.
standard absorbance range.
57 | P a g e 57
SOP OF BIO-CHEMISTRY ANALYSER
DAILY MAINTENANCE
• Check the POWER connection & WASTE BOTTLE.
• Power on the Instrument.
• Instrument will start after self check.
• First Check the flowcell position [ Is it in flowcell block]
• Instrument is ready for use.
• After completion of every test CLEAN the flowcell minimum 2 times by

pressing “ WASH ” key& using distilled water.
• NOTE : After completion of PT test don’t forget to place the flowcell in

flowcell block
• In the case of clogging clean the flow cell with Sodium Hypo chloride as
per procedure given below
*Aspirate with WASH Key Hypo chloride solution (2ml NHCl + 1ml DW)
*Wait for 10 min
*Rinse the flow cell with DW completely.
(For smooth working of the instrument the above Cleaning procedure should be done
Daily.)
WEEKLY MAINTENANCE
• Clean outer body for any stain / dirt on the analyzer surfaces.
• Check PRINTER Paper roll.
• As Per work load of lab , Daily Maintenance like Clean Flow cell /
Run controls can be done twice / thrice in a week.
• Check UPS output as per specification & verify the back-up period by
Putting the mains supply OFF. Recharge the batteries after
Verification & record the time in weekly Maintenance log sheet
58 | P a g e 58

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RD-50 User's Manual

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USER’S MANUAL

The RD-50 Chemistry Analyzer is a programmable analyzer, capable of providing

Available methods are:

The RD-50 Chemistry System analyzer: Some Facts and Features

2.2 LIST OF CONTENTS

2.3 IDENTIFICATION OF THE COMPONENTS

1. LCD Display 5. Printer Paper Roll 9. Sliding Cuvette Section

2.4.2 INSERTING PRINTER PAPER ROLL

2.5 CONNECTION TO POWER SUPPLY AND STARTING

2.5.1 QUALITY OF THE ELECTRICAL SUPPLY

It is very important to connect the instrument to a good electrical system. It should be as

2.5.3 CONNECTION TO THE POWER SUPPLY

Wait System Booting ……..

Warm up time: 180 sec

Testing Optical System…

Aspirate 1 ml of distilled water

Company :- RECKON DIAG.PVT.LTD.

Time:04:04:31 Date: 29/01/15

WASH: Press this key to rinse the flow cell.

Arrow Keys: ◄/► ▼/▲ use as a move cursor.

Enter/yes: This key allows user to select any test.

Esc/No: key to abort test and return to main/ main screen.

4.1 PROGRAMING A TEST

The following are the steps/procedure to program a test:

PROGRAM To program/edit a key board test, press the corresponding

PROGRAM [XXX] SCREEN WITH ENTERED LOCATION

XXX: Indicates the entered location number of a test.

PROGRM [ XXX] Enter the test name using the alpha

KEYBOARD TEST:GLUC Press ▼/▲ keys to navigate.

PROGRAM [XXX]: ABCD Press ▼/▲ keys to navigate.

DISPLAY PAGE 1:-

*conc: ENT/ESC ◄/► ▼/▲ concentration.

SELECTING METHOD AND SUB METHOD:

1. END POINT Press ▼/▲ to navigate.

1 WITH STANDARD Press ▼/▲ to navigate.

SELECTING WAVE LENGTH:

mg/dL Press ▼/▲ to navigate.

25 Press ▼/▲ to navigate.

*RT: Room Temperature.

Please Enter value between (3-10):

1. 100.00 Press ▼/▲ to navigate.

EDITING OF STANDARD CONCENTRATION:

Press <Enter/YES>, to edit standard concentration value.

Press <Enter/YES>, to edit factor value.

SELECTING REACTION SLOPE:

Press <Enter/YES> to select reaction slope.

1 Increasing. Press ▼/▲ to navigate

ENT ESC ◄/► ▼/▲

SELECTING NUMBER OF DECIMAL VALUE:

EDITING OF DELAY TIME:

EDITING OF READ TIME:

EDITING OF DELTA TIME:

EDITING OF REAGENT VOLUME 2:

EDITING OF SAMPLE VOLUME:

EDITING OF ASPIRATE VOLUME:

NOTE: Valid aspirate volume range is: 250 to 2000 µL

EDITING OF STARTING ABSORBANCE:

ENT / ESC ◄/►▼/▲

Press ► key to view/edit next page.

EDITING OF NORMAL MAXIMUM VALUE:

EDITING OF EXTERNAL INCUBATION TIME:

SELECTING OF PRINTER ON/OFF:

1 Printer ON Press <Enter/YES>