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Staphylococcus Aureus GM+ Cocci: Virulent
Staphylococcus Aureus GM+ Cocci: Virulent
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Streptococcus viridans (GABHS) (nonmotile, nonsporeforming) Gm+ cocci
Diseases Characteristics Habitat/Transmission Pathogenesis Diagnosis Treatment Prevention
*Pharyngitis- “strep throat”, Gm + cocci in Human throat/skin, Hyaluronidase- degrades All Strep are Penicillin to
erythema, tonsillar exudate, fever chains or pairs Transmission by proteoglycans Catalase – prevent
*Skin/soft tissue infections- respiratory droplets (TISSUE SPREAD) rheumatic
impetigo, cellulitis, necrotizing Beta-hemolytic are Erythrogenic toxin- Beta hemolysis fever.
fascitis classified by scarlet fever, lysogenized and Bacitracin
*Scarlet fever- centrifugal, red Lancefield groups S.pyogenes sensitivity point to Penicillin
rash, erythrogenic toxin, slap cheek, (A,B,D) according Streptolysin 0- results in GABHS, esp with DOES NOT
strawberry tongue to C- beta hemolysis, target of inc. ASO titer. treat post strep
*Tox shock syndrome- clinically carbohydrates ASO antibodies disease or
like Staph TSS enterococcus.
*Rheumatic fcver- fever, M protein- antibody
myocarditis, polyarthritis, chorea, target, but inhibits
subcutaneous nodules, erythema complement/phagocytosis
marginatum rash. Mitral valve Streptokinase- converts
disease follows pharyngitis, NOT plasminogen to plasmin,
skin infections. Abs vs. bacteria dissolves fibrin clots
cross react w/ joint and heart IgA protease
antigens
*Acute GN- hypertension, “HE’S an MSI”
hematuria, edema of face/ankles.
Follows both pharyngitis AND skin
infections. Cross reactive antigens
deposited in GBM.
S. agalactiae (Group B strep)
Neonatal menigitis, sepsis Beta-hemolytic Female urinary tract
pneumonia
S. faecalis (enterococcus)
Subacute endocarditis, UTI Not hemolytic GI tract Grows in 6.5% NaCl
“Oh crap! I’ve got Heart
problems!”
S. bovis (group D)
UTI Not hemolytic GI tract Hydrolyze esculin in presence of
bile. NOT grow in 6.5% NaCl
S. pneumoniae (pneumococcus)
Lobar pneumonia, ADULT Alpha-hemolytic Nasopharynx 85 different capsular Quellung rxn 23 valent vaccine, for
meningitis, URI (kids) polysaccarides Optochin sensitive AIDS, elderly, asplenics
S. Mutans , mitis (Viridans group)
Subacute endocarditis, caries Alpha-hemolytic Oropharynx Optochin resistant
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Listeria monocytogenes (Facultative intracelluar anerobes, Non-sporeforming, tumbling motility) Gm+ Rods
Diseases Characteristics Habitat/Transmission Pathogenesis Diagnosis Treatment Prevention
Neonatal meningitis and Gm + rods, in Newborns, Only Gm+ with LPS Gm+ rods, beta Ampicillin No vaccine
sepsis, abortion, premature clumps, Chinese immunocompromised are Infects monocytes and hemolysis, motility
delivery characters, NON- high risk groups. induces granulomas.
sporeforming, Listeriolysin O punches
Tumbling Transmitted to humans holes in cells
distinguishes it from animal feces,
from veggies, unpasteurized
corynebacterium milk/cheese.
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ENTERIC GRAM NEGATIVE RODS
Note: Not all gram negative enterics belong to Enterobacteriaciae family: 1) colonic location 2) facultative anaerobes 3)ferment
glucose, 4)oxidase negative, and 5)reduce nitrates to nitrites. ALL are members here EXCEPT: Vibrio, Campylobacter,
Helicobacter, Pseudomonas, Bacteriodes. (“Vile People Can’t Be Happy”) As a group, Enterobacteriaciae are often normal flora.
Pathogenisis is by endotoxin/LPS, exotoxins. O (Outer polysaccharides), H (flagHella), K (Kapsular polysaccharides) are important
antigens. Inoculation on MacConkey’s or Eosin-Methylene Blue (EMB) agar differentiates family members by lactose fermenting
ability. Fermenters are pink-purple, non-fermenters are colorless. Also keep an eye on motility.
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H.Simpson
Legionella pneumophilia (Cysteine and Iron agar)
Atypical pneumonia with high Poor gm stain Airborne from water High concentration Erythromycin Disinfect water
fever, nonproductive cough( sources. Smoking EtOH, .of cysteine and (also good for sources
differentiate from Mycoplasma, Immunosuppressed are iron. Urine antigen Mycoplasma)
influenza, psittacosis, Q fever) at risk. test. Suspect when
inc. PMNs with no
organisms!
Bordetella pertussis (Bordet-genou agar)
Whooping cough- acute Small gm- rods Airborn droplets (highly Polysaccharide capsule Culture on Bordet- Erythromycin Killed B.pertussis
tracheobronchitis with URI contagious) and pili are essential for genou agar. Ab reduces vaccine 2,4,6
symptoms, paroxysmal virulence. Does NOT agglutination, stain complications, months, Boosters
hacking cough 1-4 wks, invade. Pertussis tox doesn’t change at age 1, school.
copious mucus (ADP-ribosylation), and clinical course. Acellular vax for
tracheal cytotoxin. Resp tract booster only.
already
damaged.
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MYCOBACTERIA (Obligate aerobe, facultative intracellular organisms) Acid Fast Rods
M. tuberculosis
Diseases Character Habitat/Transmission Pathogenesis Diagnosis Treatment Prevention
Tuberculosis: chronic low Obligate Only infects humans. Respiratory Mycolic acids confer PPD tests for prior Prolonged, Chemoproph
grade fever, night sweats, aerobe, aerosol. Infects M0s in mid/lower acid fastness. WaxD is exposure or to BCG multiple Tx. ylax w/ INH
productive cough, hemoptysis, intracellular, lobes, init granuloma formation, active ingredient in vax. Positive test if (INH, rifampin, (watch
weight loss. Elderly, infect M0, widely disseminate the infection. T Freund’s adjuvant. both redness, pyrazinamide, hepatotox in
immunocomp, malnourished at persist for cells help M0 kill some intracellular Cord factor is induration 48-72 ehtambutol) people >35
risk. years. mycobacteria at expense of virulence factor hr after injection y.o.)
Mycolic bystander cell damage. Result: (mycoside= 2 mycolic (DTH rxn.) Protracted tx
acid walls necrotic host cells, viable acids + disaccharide) b/c: intracellular Live
mycobacteria. Walled off w/ giant Note: candida and life cycle, attenuated
cells, fibroblasts, collagen, mumps as controls granuloma M.bovis
calcification to form granuloma or in immunocomp. blocks (BCG)
tubercle. This 1’ infection = Ghon penetration of induces some
focus on CXR (when including Ca Acid fast stain. drug, protective
tubercles in perihilar lymph nodes = NaOH concentrate metabolically immunity.
Ghon complex.) Reactivation on Lowenstein- inactive
prefers upper lobe (obligate Jensen medium. mycobac persist
aerobe) of lung. Reactivation can Slow culture, 6-8 in lesion
infect any organ. Cervical LN wks. Niacin
(scrofula),spine (Pott’s disease.) production
M. avium- intracellulare
Clincal TB indistinguishable Atypical Found in water, soil, not Azithromycin Macrolide
from M tuberculosis in AIDS. mycobacterium pathogenic in guinea pigs Clarithromycin prophylax
(infects birds) when CD4
count < 50
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M. leprae
Leprosy- preferential growth in < 37C, Never has Brazil, India, Sudan Intracellular replication Rifampin Prophylax
skin, superficial nerves. been (skin histiocytes, Dapsone exposed
Tuberculoid- good cellular immune grown in Humans only natural hosts. endothelial cells, Up to 2 years! persons with
response, few AFB, granulomas, lab. Mouse footpad and Schwann cells) Dapsone.
positive lepromin skin test. Anethetized armadillo growth only.
skin lesions and thickened superficial Transmission by nasal
nerves. secretions, skin lesions to
Lepromatous- poor cellular immune persons with prolonged
response, lots of organisms, foamy contact w/pts.
histiocytes, negative lepromin skin test
(poor response.) Skin lesions, lion
facies. Skin anesthesia, bone resorption,
skin thickening, disfiguring.
Mycoplasma pneumoniae (No cell wall, poor gm stain) Small free living organism
Diseases Characteristics Habitat/Transmission Pathogenesis Diagnosis Treatment Prevention
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“Walking pneumonia” Smallest free living Respiratory droplets. Pathogenic only for Elevated titer of cold Erythromycin No vaccine
(dry nonproductive cough, organism, no cell Attaches but does NOT humans. agglutinins or specific Tetracycline
horrible CXR, generally wall so poor gm invade respiratory anitbodies
feel well) Most common stain, resists epithelium, like Arrests cilliary motion,
pneumonia in young penicillins, B.pertussis. induces epithelial cell
adults (college students). cephalosporins. necrosis. Cross reactive
Cell membrane has antigens induce anti RBC
chol which are not autoantibodies (cold
in other bacteria. agglutinins.)
“Fried egg”
colonies on Eaton’s
agar. (“Eat Fried
Eggs w/ chol”)
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1’ syphillis – 6 wks post infection, highly Thin-walled Human genital Immunopath Darkfield microscopy Early disease by Prophylax in
infectious, nontender chancre, many organisms, flexible spiral tract ogenesis in early lesions; VDRL, single injection sexual
“cures” in 6 weeks rods, tight spiral mediated by RPR for screening, and of bezathine contacts.
2’ syphillis – 6 wks post chancre healing, spriochetes inflammatory monitoring response to penicillin G.
disseminates to organs (hepatitis), maculopapular cell therapy. High No vaccine
lesions on palms, soles oral mucosa. Condoloma infiltrates, sensitivity, low Erythromycin
latum may arise. Noninfectious stage, serology granuloma specificity. VDRL
positive, 66% resolve in 6 wks, 33% go to 3’. formation, titers decline as
3’ syphilllis – six years later, reactivation, bone and vascular syphillis is treated.
skin granulomas (gummas), aortic aneurysm , proliferation
meingitis, circle o Willis infarcts, tabes dorsalis Use FTA-ABS test to
(post column/dorsal root damage, loss of vibration, rule out false positive
pain, temp, position sense) Treptonemes rare in VDRL, RPR.
these lesions. FTA-ABS turns
Congenital syphillis- transmitted to fetus AFTER 1st positive earliest in the
trimester. Stillbirth, fetal abnormalities disease.
Borrelia burgdorferi (most common vector borne disease in US)
Lyme disease- 1) Erythema chronicum migrans, Big, spirals White footed mouse Bloodstream Darkfield Doxycycline Stay out of the
spreading circular red rash w/ clear center at bite reservoir, deer tick spread microscopy w/ Amoxicillin woods!
appears 10 days post bite, lasts 4 wks. 2) Ixodes as vector Giemsa stain
Myocarditis- pericarditis, meningitis, cranial nerve
palsy 3) arthritis in large joints, chronic Diagnose w/
progressive CNS involvement ELISA antibody
Borrelia recurrentis
Relapsing fever- chills, headache, multiple organ Big spirals Human hosts, rodent Large spirochetes Doxycycline
dysfunction reservoir, human in Giemsa blood
body louse vector smears. No
serology
Leptospira interrogans
Leptospiriosis- fever, chills, headache to meningitis. Tight coils Transmission by Penetrates Tightly coiled Penicillin
Also Weil’s disease- (infectious jaundice) – swimming/ contaminated abraded skin. spirochetes by Doxycycline
hepatitis, renal failure, alt mental status, hemorrhage. food, water. Water, soil darkfield, rise in
contaminated by urine of antibody titer
infected rats, livestock,
pets. Sewage workers,
farmers at high risk.
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CHLAMYDIAE Obligate Intracellular Parasites
C. trachomatis
Diseases Character Habitat/Transmissio Pathogenesis Diagnosis Treatment Prevention
n
Types A-C cause trachoma, chronic 15 Humans only, Obligate Giemsa of Treat like
conjuctivitis esp. Asia, Aftrica. Leading serotypes sexual contact, intracellular conjuntival mixed
cause of preventable blindness.
Inflammation, scar retraction, eyelashes passage through parasites (require scraping infection w/
scratch cornea, lead to bacteria birth canal. Finger- host ATP) , inert ceftriaxone
superinfection, blindness. fomite-eye contact. elementary body (G3),
Types D-K – genital tract enters cell, inhibits doxycycline
infections- nongonococcal Dirty towels. phagolysosome
urethritis (NGU), PID, infertility, fusion, reorganizes
ctopic, tuboovarian abscess, into metabolically
peritonitis, perihepatits (Fitz-hugh- active reticulate
curtis), neonatal inclusion body. Undergoes
conjunctivitis (erythromycin binary fission to
prophylax), pneumonitis. produce daughter
Clinically like gonorrhea. elementary bodies.
Types L1-L3 cause
Lymphogranuloma venereum,
tender lesions on genitalia, draining
lymph nodes
C. psittaci
Atypical pneumonia, psittacosis Infects birds/
mammals. Inhaled
from dry bird crap or
feathers
C. pneumoniae
Atypical pneumonia, dry nonproductive Respiratory droplets
cough, lack of physical findings, diffuse
infiltrates on CXR
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