kT = k20(θ)T−20 

where T = temperature of interest (in ◦C)

kT = BOD rate constant at the temperature
of interest (in days−1)
k20 = BOD rate constant determined at
20◦C (in days−1)
θ = temperature coefficient. For typical
domestic wastewater, this has a value of
1.135 for temperatures between 4 and
20◦C and 1.056 for temperatures between
20 and 30◦C.

where DOb,t and DOs,t are defined earlier and

DOs,i = the initial DO of the sample
DOb,i = the initial DO of the blank (seed) control
f = ratio of seed in diluted sample to seed in seed
control
= (% seed in diluted sample)/(% seed in seed
control)
= (volume of seed in diluted sample)/(volume of
seed in seed control)