GROUP ASSIGNMENT

CASE 1

◦ A male patient 40 years old, came to hospital with complaints of pain in the armpit, on physical

examination the doctor found a red lump 2x3 cm, with a clear appertum punctured, accompanied by

enlarged glands.

◦ What actions do you take as a microbiologist? (Proposed examination, possible results expected?)

◦ If in the above case gram-positive cocci was found, what would you do next?

◦ If the culture results are as below, what antibiotics are not recommended for this patient?
CASE 2

◦ If the results of the examination of 2 positive catalase-positive gram cocci bacteria colonies from 2

different people show the results:

◦ Colony A: coagulase negative

◦ Colony B: positive coagulase

◦ Both colonies tested for novobiocin and both were positive

What do you think?

INDIVIDUAL ASSIGNMENT

1. Describe the procedure and mechanism of action of the catalase test

2. Describe the procedure and mechanism of action of the coagulase test

3. Describe the procedure and mechanism of action of the novobiocin test

4. Describe the procedure for taking a serum reitz sample

 TUGAS KELOMPOK

KASUS 1

◦ Seorang pasien lelaki usia 40 th, datang ke tempat praktek saudara dengan keluhan nyeri di

ketiak, pada pemeriksaan fisik didapatkan benjolan merah 2x3 cm, dengan pungtum appertum

yg jelas, disertai pembesaran kelenjar.

◦ Tindakan apa yg saudara lakukan sebagai ahli mikrobiologi? (Usulan pemeriksaan, kemungkinan

hasil yg diharapkan?)

◦ Bila pada kasus di atas ditemukan bakteri kokus gram positif, pemeriksaan yg saudara lakukan

selanjutnya?

◦ Bila hasil kultur seperti di bawah, antibiotika apa yg tidak disarankan untuk pasien tersebut?
 KASUS 2

◦ Bila hasil pemeriksaan dari 2 koloni bakteri kokus gram positif katalase positif dari 2 orang yg

berbeda menunjukkan hasil :

◦ Koloni A: koagulase negatif

◦ Koloni B: koagulase positif

◦ Pada kedua koloni dilakukan uji uji novobiocin keduanya sama2 positif

Apa pendapat saudara?

TUGAS INDIVIDU

1. Jelaskan prosedur dan mekanisme kerja uji katalase

2. Jelaskan prosedur dan mekanisme kerja uji koagulase

3. Jelaskan prosedur dan mekanisme kerja uji novobiocin

4. Jelaskan prosedur pengambilan sampel reitz serum