14-Sep-20

BAKTERIOLOGI INFEKSI KULIT &

JARINGAN LUNAK

EKO BUDI KOENDHORI

KUNTAMAN

DEPARTEMEN MIKROBIOLOGI KLINIK,

FAKULTAS KEDOKTERAN ,UNIVERSITAS AIRLANGGA

POKOK BAHASAN

• STRUKTUR JARINGAN LUNAK &

PENYAKIT INFEKSI
• STAPHYLOCOCCUS SPP
• MYCOBACTERIUM LEPRAE

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SOFT TISSUE

INFECTION OF SOFT TISSUE

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DISEDIAKAN
• UJI KATALASE
• STAPHYLOCOCCUS : • UJI KOAGULASE
•
•
S. AUREUS
S. EPIDERMIDIS
• MANNITOL SALT AGAR
• S. SAPHROPHYTICUS
(MSA)
• NOVOBIOCIN TEST
• MRSA IDENTIFICATION
• MYCOBACTERIUM LEPRAE :
• SLIDE -MICROSCOPIC

IDENTIFIKASI STAPHYLOCOCCUS

• UJI KATALASE (CATALASE TEST)

• UJI KOAGULASE (COAGULASE TEST)
• MANNITOL SALT AGAR (MSA): FERMENTATION TEST
• NOVOBIOCIN TEST (SUSCEPTIBILITY TEST)

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STAPHYLOCOCCUS SPP: MORPHOLOGY

USE OF CATALASE TEST

CATALASE TEST IS PRIMARILY USED TO
DIFFERENTIATE AMONG GRAM-POSITIVE
COCCI
• STAPHYLOCOCCUS : ARE CATALASE-POSITIVE,
• GROUP OF STREPTOCOCCCI (i.e.: Streptococcus
pyogenes) AND ENTEROCOCCI (i.e.: Enterococcus
faecalis) Are CATALASE-NEGATIVE.

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CATALASE TEST
METHOD :
• SLIDE (DROP) METHOD
• TUBE METHOD
• TUBE (SLANT) METHOD

CATALASE TEST (SLIDE TEST)

Gass of O2
bubble

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CATALASE TEST
Catalase POS Catalase NEG

Staphylococcus

CATALASE TEST (TUBE METHOD)

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CATALASE TEST (TUBE (SLANT)

METHOD)

CATALASE TEST VIDEO

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COAGULASE TEST
• Differentiatesstrains of staphylococcus aureus
from S. Epidermidis and other coagulase-
negative species
• S. aureus strains are usually capable of
coagulating edta-treated plasma in the tube test
and will produce clumps of cells in the slide test.

COAGULASE TEST

• METHOD :
• SLIDE TEST
• TUBE TEST

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COAGULASE TEST (SLIDE TEST)

Differential
Characteristics
S. aureus
Coagulase
Fibrinogen ------------ Fibrin

Agglutination

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Staphylococcus aureus

Coagulase POS

Coagulase NEG

COAGULASE TEST VIDEO

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MANNITOL SALT AGAR

• FORMULA OF MEDIUM :
• MANNITOL
• PHENOL RED AS A PH INDICATOR (PH = 7.8)
• REACTION
• MANNITOL FERMENTED  ACID PRODUCED
• PH < 6.9 : YELLOW COLOR
• PH > 8.4 : RED COLOR

VARIOUS SPESIES ON
TRIPTICASE SOY AGAR (TSA)

A.S. AUREUS
B.S. EPIDERMIDIS
C.E. COLI

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VARIOUS SPESIES ON
MANITOL SALT AGAR (TSA)

A. S. AUREUS
B. S. EPIDERMIDIS
C. E. COLI

NOVOBIOCIN TEST

• DIFFERENTIATE BETWEEN S. EPIDERMIDIS W/ S.

SAPROPHYTICUS
• USING NOVOBIOCIN DISC ( 5 G)
•  < 15 MM = RESISTEN  S. SAPROPHYTICUS

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NOVOBIOCIN TEST

IDENTIFIKASI MRSA
• MRSA : Methicillin-resistant Staphylococcus aureus
• Methicillin  Oxacillin  Cefoxitin
• CLSI M100
• Using Cefoxitin Disc :
•   21 Mm : MRSA
•  > 21 Mm : Non – MRSA
• MIC ≥ 4 μg / ml

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SUSPECT MRSA

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COLLECT SAMPLE FROM WOUND

COLLECT SAMPLE FROM NOSE

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MYCOBACTERIUM LEPRAE

SAMPLING :

1. SKIN SCRAP USING SCALPEL (Lesion

Hypopigmentation & numb skin = mati rasa )
2. REITZ SERUM
3. NOSE SCRAPPING

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STAIN
•Make glass slide for microscopic examination
•Stain with Ziehl-Neelsen (ZN) stain method
•M leprae: acid fast bacilli/rod; red color
•Presence of globi (singular = globus):
encapsulated amphophilic masses of non-viable
bacilli found within macrophages or in
extracellular space

How to read the microscopic examnation of

M leprae
• RESULT : (BACTERIAL INDEX)
1+ : at least 1 bacillus in every 100 fields.
2+ : at least 1 bacillus in every 10 fields.
3+ : at least 1 bacillus in every field.
4+ : at least 10 bacilli in every field.
5+ : at least 100 bacilli in every field.
6+ : at least 1000 bacilli in every field.

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