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    This document outlines a 4-day bacteriology laboratory practical involving the identification and isolation of gram-positive cocci. Day 1 involves preparing culture media. Day 2 involves identifying staphylococci grown on NB as gram-positive cocci using microscopy and catalase testing, then transferring the bacteria to BAP and incubating. Day 3 includes a positive catalase test on the BAP cultures, gram staining and identifying them as staphylococci, and isolating them on MSA. Day 4 involves a positive coagulase test identifying S. aureus and observing coagulation. Key techniques include isolation, identification, catalase and coagulase testing to distinguish staphylococcal species, and hemolytic testing

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    Poltekkes Kemenkes Mataram

    Original Title

    DOKUMENTASI PRAKTIKUM BAKTERIOLOGI

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    This document outlines a 4-day bacteriology laboratory practical involving the identification and isolation of gram-positive cocci. Day 1 involves preparing culture media. Day 2 involves identifying staphylococci grown on NB as gram-positive cocci using microscopy and catalase testing, then transferring the bacteria to BAP and incubating. Day 3 includes a positive catalase test on the BAP cultures, gram staining and identifying them as staphylococci, and isolating them on MSA. Day 4 involves a positive coagulase test identifying S. aureus and observing coagulation. Key techniques include isolation, identification, catalase and coagulase testing to distinguish staphylococcal species, and hemolytic testing

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    4 views4 pages

    Dokumentasi Praktikum Bakteriologi

    Uploaded by

    bismillahgusti cumlaude
    This document outlines a 4-day bacteriology laboratory practical involving the identification and isolation of gram-positive cocci. Day 1 involves preparing culture media. Day 2 involves identifying staphylococci grown on NB as gram-positive cocci using microscopy and catalase testing, then transferring the bacteria to BAP and incubating. Day 3 includes a positive catalase test on the BAP cultures, gram staining and identifying them as staphylococci, and isolating them on MSA. Day 4 involves a positive coagulase test identifying S. aureus and observing coagulation. Key techniques include isolation, identification, catalase and coagulase testing to distinguish staphylococcal species, and hemolytic testing

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    of 4

    DOKUMENTASI PRAKTIKUM BAKTERIOLOGI

    IDENTIFIKASI BIAKAN BAP IDENTIFIKASI MSA UJI KATALASE

    MAKROSKOPIS NAP MAKROSKOPIS BAP MAKROSKOPIS


    MSA

    MIKROSKOPIS NAP MIKROSKOPIS BAP MIKROSKOPIS


    MSA
    REVIEW PRAKTIKUM
    Day 1
    Pembuatan NA
    Pembuatan MSA
    Pembuatan NB
    Penanaman bakteri pada sediaan NB
    Diambil sampel nanah dari luka pasien dengan menggunakan cuttonbad dan ditanam pada
    media NB, lalu diinkubasi 18-24 jam.

    Day 2
    a. Identifikasi Sediaan Bakteri Pada media NB
    Makroskopis: Keruh
    Mikroskopis: Coccus Gram Positif (Stapilococcus)
    b. Pembuatan Sediaan BAP
    BAP= NA+Golongan darah O
    c. Penanaman bakteri pada NB ke sediaan BAP diinkubasi 24 jam
    Day 3
    a. Uji Katalase (H202+Sediaan bakteri BAP)
    b. Identifikasi Sediaan BAP
    Pz+satu oase bap lalu pewarnaan gram
    Makroskopis: jernih
    Mikroskopis: stapilococcus
    c. Isolasi BAKTERI BAP ke sediaan MSA

    Day 4
    a. Uji Koagulase (serum darah+msa)
    b. Kalo ada getah brty sta. Aureus

    MATERI PRAKTIKUM
    [MATERI BAKTERIOLOGI ]
    #Identifikasi dan Isolasi COCCUS GRAM POSITIF
    # Isolasi adalah=Suatu kegiatan untuk mengumpulkan bakteri dengan cara isolasi atau kultur.
    (Penanaman) Identifikasi adalah Suatu Kegiatan untuk memilih/menentukan.
    Coccus (+)
    Genus Stapilococcus (+)
    Genus Streptococcus (-)
    Dibedakan dengan uji Katalase
    Uji Katalase -Teteskan setetes H202 3% keep object glass -Satu oase biakan bakteri BAP (+)
    Ada gelembubg #Stapilococus Ada gelembung#
     Stapilococus dibagi menjadi 3
    -Sta. Aureus (+) Terangkat seperti getah
    -Sta. Epidermis (-)
    -Sta. Sapropiticus (-)
    Cara Identifikasi= Uji Koagulase Uji Koagulase: +Setetes Serum Segar +Setetes biakan bakteri
    MSA (+) Ada getah
    # S.aureus positif dengan Ada getah#
    Cara membedakan uji koagulase negatif Uji Antibiotic Novobiocin
    S. Epidermis is: Sensitive (S)
    S. Sapropiticius: Resistan (R)
     Streptococcus (Terjadi hemolisa ya itu: pecahnya sel darah merah dengan mengubah
    hemoglobin menjadi meta hemoglobin) dibedakan menjadi tiga:
    -alpha Hemolisa (Hemolisa Sebagian)(Zona Hijau)
    ! St. Pneumonia (S)
    ! St. Viridans (R)
    Uji antibiotic: Optochin
    -Beta Hemolisa (Hemolisa Total) (Zona Kuning)
    ! St. Pyogenes (s)
    ! St. Agalactiae (r)
    Uji antibiotic: Bacitracin
    -Gama Hemolisa (Non Hemolisa) (Jernih/Putih)
    !Enterococcus]

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