NAME:GANTALAO, JOHANNA MARIE R.

Date: 19/11/20 SCORE:__________

Lab. teacher: Prof. Salina Alfad Course & Section: BSN-1B Grp. No. 3

ACTIVITY N0. 10
EFFECT OF pH ON ENZYME ACTIVITY
Results:

Time in Tube 1 Tube 2 Tube 3

Minute
s Iodine Benedict’s Iodine Benedict’s Iodine Benedict’s

30 + _ _ + (red) + _

15 + _ _ + (red) + _

+
15 _ _ + (red) + _

15 + _ _ + (red) + _

15 + _ _ + (red) + _

15 + _ _ + (red) + _

15 + _ _ + (red) + _

Note: Mark ( +) if the blue color appears and (-) if no blue color appears in iodine test.
(+) if precipitate is formed and note the color of the ppt. and (-) if no precipitate is
formed in benedict’s test.

TUBE
1:
Acidic
NAME:GANTALAO, JOHANNA MARIE R. Date: 19/11/20 SCORE:__________
Lab. teacher: Prof. Salina Alfad Course & Section: BSN-1B Grp. No. 3

ACTIVITY N0. 10
EFFECT OF pH ON ENZYME ACTIVITY

Question Guide:
1. In which tube is there evidence of unhydrolyzed starch after 30 minutes?
The test tube numbers 1 and 3 showed an unhydrolyzed starch after 30 minutes since for
tube 1, the substance added was acid which will cause the decline in the enzymatic
activity same with the tube 3 which is basic. These tubes contain pH that did not reach
(tube 1) and exceeded (tube 3) the optimum pH which is 6-7 that is why the starch
remained unhydrolyzed because of the low activity of enzymes caused by the
denaturation.
2. What is the action of acid and alkali on the enzyme in saliva?
Acid: The pH level of the saliva can drop below 5.5 when come in contact with acid.
When this happens in the mouth, the acids in your mouth start to demineralize (break
down) tooth enamel. When the food is swallow to the Stomach it secretes acids, acid
does not digest protein. Rather, it activates an enzyme called pepsinogen which then
becomes pepsin that is secreted by the stomach wall. This enzyme is only active within
the pH range of 3.0 to 5.0 and requires the acid to maintain that pH. Pepsin is very
specific in its action and is simply incapable of digesting food enzymes, which are very
large molecules and are more than just protein
Alkali___________________________________________________________________
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
_________________________________________________________

3. What is the optimum pH of the enzyme in the saliva?

The optimum pH for the enzymatic activity of salivary amylase ranges from 6 to 7.
Above and below this range, the reaction rate reduces as enzymes get denaturated. The
enzyme salivary amylase is most active at pH 6.8. Our stomach has high level of acidity
which causes the salivary amylase to denature and change its shape. So the salivary
amylase does not function once it enters the stomach.