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The effects of inlet temperatures of 125, 150, 175 and 200 ◦ C and maltodextrin levels at 3, 5, 7 and 9% on the physico-
chemical properties, total phenolic content (TPC) and 2,2-diphenyl picryl hydrazile (DPPH) scavenging activity of spray
dried amla juice powder were studied. Moisture content and hygroscopicity of powder were significantly affected
by inlet temperature and maltodextrin level. However, an increase in the level of maltodextrin did not significantly
affect the bulk density and water solubility index (WSI). An increase in drying temperature and maltodextrin con-
centration decreased the free radical scavenging activity of the powder. Morphological study revealed that at higher
inlet temperatures the spray dried powder had small sized particles that were densely packed. Spray dried amla juice
powder made with 7% maltodextrin and processed at 175 ◦ C inlet temperature had less hygroscopicity, acceptable
color and potent free radical scavenging activity.
© 2013 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
∗
Corresponding author. Tel.: +91 03712 267007x5705; fax: +91 03712 267005/267006.
E-mail address: poonam@tezu.ernet.in (P. Mishra).
Received 12 May 2013; Received in revised form 30 July 2013; Accepted 1 August 2013
0960-3085/$ – see front matter © 2013 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
http://dx.doi.org/10.1016/j.fbp.2013.08.003
food and bioproducts processing 9 2 ( 2 0 1 4 ) 252–258 253
agent. Workers have used carrier agents to protect heat sensi- 2.4. Analytical methods
tive components like vitamin C in camu-camu (Dib-Taxi et al.,
2003) and to increase stability of products in acerola pow- 2.4.1. WSI (water solubility index)
der (Righetto and Netto, 2005). However, there are no reported The WSI of the powder was determined using the method
studies on spray drying of amla juice powder to retain func- described by Anderson et al. (1969). Spray dried amla juice
tional properties and health promoting factors. powder (2.0 g) and distilled water (25 mL) were vigorously
In the present study, amla juice was spray dried with mixed in a 100 mL centrifuge tube, incubated in a 37 ◦ C water
different maltodextrin concentrations at different inlet tem- bath for 30 min and then centrifuged for 20 min at 10,000 rpm
peratures. The effect of spray drying on the physicochemical (Sigma, 13 K, Germany). The supernatant was carefully col-
properties, total phenolic content and free radical scavenging lected in a pre-weighed beaker and oven dried at 103 ± 2 ◦ C.
activity of spray dried amla powder are reported. The WSI (%) was calculated as the percentage of dried super-
natant with respect to the amount of the original amla powder.
Maltodextrin concentration 3 ND ND ND ND
(MDC) (%) 5 5.6 + 0.11a 53.01 + 1.32a 93.28 + 2.33a 0.52 + 0.01a
7 4.07 + 0.15b 46.74 + 1.24b 94.11 + 1.87a 0.49 + 0.03a
9 3.83 + 0.42c 46.03 + 0.98c 93.32 + 2.41a 0.51 + 0.04a
Aspiration speed (AS) 30 3.83 + 0.42a 46.74 + 1.33a 94.89 + 2.37a 0.49 + 0.03a
40 4.01 + 0.39b 47.08 + 2.09a 93.28 + 2.01a 0.52 + 0.18a
50 4.32 + 0.54c 47.34 + 1.49a 91.43 + 2.52a 0.52 + 0.23a
Significant interaction
** ***
MDC NS NS
*** ** ***
DT NS
MDC × DT ** **
NS
ND: not detected; NS: non significant. The values of the same column with different superscript differ significantly (p < 0.05).
∗∗
Significant at p = 0.01.
∗∗∗
Significant at p = 0.001.
the sample with methanol. DPPH radical scavenging activity sample decreased from 5.6 to 3.8% (Table 1). Abadio et al.
of the sample was calculated as follows: (2004) observed that on increasing the level of maltodextrin
from 10 to 15% (w/v), there was decrease in moisture content
DPPH radical scavenging activity (%) in final pineapple juice powder. Increased level of maltodex-
trin increased the level of feed solids and reduced the level
absorbance of control − absorbance of sample
= × 100 of total moisture for evaporation (Grabowski et al., 2006; Kha
absorbance of control
et al., 2010).
A similar trend was found while increasing drying temper-
ature from 125 ◦ C to 200 ◦ C, resulting in significant decrease
2.5. Scanning electron micrograph in the moisture content in amla powder from 6.3 to 3.8%
(Table 1). Due to the increased rate of heat transfer into the
Particle morphology was evaluated by scanning electron particles at higher temperature, there was a greater driving
microscope (SEM). Powders were attached to a double sided force for moisture evaporation causing faster water removal
adhesive tape on SEM stubs, coated with 3–5 mA palladium (Quek et al., 2007; Solvol et al., 2012). The present find-
under vacuum and examined with a JEOL scanning elec- ings were in agreement with the results obtained for spray
tron microscope (JSM-6390 LV, Japan, PN junction type, semi dried tomato powder (Goula et al., 2004), orange juice pow-
conducting detector). SEM was operated with 15 kV at magni- der (Chegini and Ghobadian, 2005), cactus pear juice powder
fication of 1000× and 5500×. (Rodriguez-Hernandez et al., 2005), black carrot powder (Ersus
and Yurdagel, 2007) and gac juice powder (Kha et al., 2010).
2.6. Statistical analysis Maltodextrin level had significant (p < 0.05) effect on the
hygroscopicity of the amla powder. Hygroscopicity was lowest
The experiments were carried out in duplicate and obtained when 9% maltodextrin was used for encapsulation. Rodriguez-
mean values were analyzed by analysis of variance (ANOVA). Hernandez et al. (2005) and Cai and Corke (2000) also observed
The graphs of mean value and error bars were created by using a reduction in hygroscopicity with increasing maltodextrin
Excel version of 2003. concentrations in spray dried cactus pear juice powder and
betacyanin pigments, respectively. Maltodextrin is a material
3. Results and discussion having the property of low hygroscopicity and its utility as a
carrier material for spray drying (Tonon et al., 2008) has been
3.1. Effects of spray drying conditions on physical established. Inlet temperature also influenced the hygroscop-
properties of amla powder icity of the powder significantly. The highest hygroscopicity
value of 56.32 g/100 g for amla powder was obtained at 125 ◦ C
The effects of maltodextrin concentration, aspiration speed inlet temperature. When inlet temperature of processing was
and different drying temperatures on the physical properties increased the hygroscopicity of amla powder was decreased.
of the amla juice powder are shown in Table 1. The levels The present findings are in agreement with Moreira et al.
of maltodextrin used for development of the amla powder (2009) but contradicts the findings of Goula et al. (2004) and
varied between 5% and 9% (w/v) which were less than 10–30% Tonon et al. (2008) in their work on spray drying of tomato
that were used by Tonon et al. (2008), Abadio et al. (2004), and pulp and acai juice powder, respectively. Amla powder showed
Kha et al. (2010). Increase in the maltodextrin concentration greater tendency to adsorb moisture which may be due to the
resulted in a significant (5% probability level) decrease in presence of higher level of carbohydrates in amla fruit. Aspi-
moisture concentration in the finished powder. As maltodex- ration speed showed no significant effect on hygroscopicity of
trin level increased from 5 to 9%, the moisture content of the powders.
food and bioproducts processing 9 2 ( 2 0 1 4 ) 252–258 255
Fig. 1 – Micrographs of particles at different inlet temperature and constant maltodextrin level (9%) at magnifications of (a)
150 ◦ C, 1000×; (b) 150 ◦ C, 5500×; (c) 175 ◦ C, 1000×; (d) 175 ◦ C, 5500×; (e) 200 ◦ C, 1000×; (f) 200 ◦ C, 5500×.
Drying temperature did not show any significant effect carbohydrates and the low level of fat (0.05% in fresh fruit)
on WSI of the amla powder (Table 1) at 5% probability level in the juice. According to Kumar et al. (2006), 97.67% of total
(Table 1). Sousa et al. (2008) also observed that drying con- phenolics are present in free form in amla fruit. The dissolved
ditions had no significant effect on WSI of tomato powder. solids in the amla juice are highly water soluble. These soluble
In the present study, WSI of amla powder ranged from 91.34 solids are mostly carbohydrates and free phenolics. Thus,
to 94.98%. These values were higher when compared with free phenolics and soluble carbohydrates account for the high
17.65–26.3% in spray dried tomato powder (Sousa et al., 2008), WSI in amla juice powder. The high WSI observed in amla
36.91–38.25% in gac powder (Kha et al., 2010) and 81.56% in powder makes it suitable for reconstitution.
pineapple juice powder (Abadio et al., 2004). The excellent While maltodextrin level did not have significant effect
WSI of the amla juice powder could be due to the high content on the bulk density of amla powder at 5% probability level,
of free phenolics (Kumar et al., 2006), the significant level of the drying temperature showed a significant effect (Table 1).
256 food and bioproducts processing 9 2 ( 2 0 1 4 ) 252–258
95
0.08
0.07
90
0.06
85 0.05
Lightness
0.04
a/b
80 0.03
0.02
75
0.01
0
70
1 2 3 4
125 150 175 200 -0.01
Temperature Temperature
(a) (b)
Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9% Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9%
18 92
16 91
14 90
Hue angle
89
Chroma
12
88
10
87
8
86
6
85
4
125 150 175 200
125 150 175 200
Temperature Temperature
(c) (d)
Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9% Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9%
Fig. 2 – The color characteristics of spray dried amla powder: (a) lightness, (b) ratio of a/b, (c) chroma and (d) hue angle at
different inlet temperatures and maltodextrin level.
A decrease in the density of the powder was observed with of particles in the powder that was dried at higher inlet tem-
an increase in the inlet temperature. This finding is consis- perature was smaller than the particles in powder dried at
tent with those by Walton and Mumford (1999), Cai and Corke lower inlet temperature. Similar finding was observed by Cai
(2000), Goula et al. (2004) and Kha et al. (2010). As explained by and Corke (2000) for spray drying of amaranthus betacyanin
Jumah et al. (2000), Walton (2000) and Chegini and Ghobadian pigments. Probably, the particle size got fixed as large sized
(2005), the high rate of drying being rapid at very high tem- globules when there was more water in the material that was
peratures meant that there was less droplet shrinkage, giving being dried. At higher inlet temperature, due to rapid rate of
lower powder density. This explanation is supported by our drying the particles got fixed as smaller sized globules.
findings. Aspiration speed had no significant effect on the bulk
density of the powder. 3.3. Color characteristics of powder
3.2. Particle morphology Fig. 2 shows the effect of different concentrations of maltodex-
trin and inlet temperatures on color characteristics of spray
Fig. 1 shows the SEM micrographs of amla juice powder pro- dried powder. In general, the color attributes of the powder
duced with 9% maltodextrin at different inlet temperatures. It was significantly affected by maltodextrin and temperature.
was observed that the number of particles in a given amount The lightness of the powders was significantly affected by
of the powder increased with an increase in inlet tempera- maltodextrin concentration (p < 0.01) when powders were pro-
ture. Similar findings were also reported by Tonon et al. (2008). duced at inlet temperatures below 200 ◦ C. Highest value of a/b
Inlet temperature had no effect on the surface smoothness and lowest value of hue angle were obtained with 5% con-
of the particles. This however contradicts the observation of centration of maltodextrin at 200 ◦ C while no specific trend
Allamila-Beltran et al. (2005), Nijdam and Langrish (2006) and was observed for 7% and 9% levels of maltodextrin. No specific
Tonon et al. (2008). SEM study revealed that the average size trend could be observed for chroma of powder when produced
food and bioproducts processing 9 2 ( 2 0 1 4 ) 252–258 257
(db)
200 ◦ C temperature. An increase in inlet temperature produced 150
significantly (p < 0.001) lighter product than powder produced
100
at lower inlet temperature. Greatest degree of lightness of
50
spray dried amla powder at highest inlet temperature indi-
0
cates that the pigments had undergone oxidation. Similar 125 150 175 200
results were observed by Sousa et al. (2008) in spray dried Temperature
tomato powders. The probable reason for higher degree of (a)
lightness of the amla powder at higher inlet temperature may Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9%
be attributed to the reduced rate of oxidation of the tannins.
of powder (db)
with iron as availability of oxygen is reduced and therefore
2
the powder color had lessened.
Loss of redness of samples increased, resulting in low 1.5
a/b value and high hue angle, when inlet temperature was
increased from 125 ◦ C to 175 ◦ C, however above 175 ◦ C temper- 1
ature high a/b and low hue angle was found in the powder.
0.5
Similar findings were reported by Graboswki et al. (2006) for 125 150 175 200
sweet potato powder, by Abadio et al. (2004) for pineapple juice Temperature
powder and by Kha et al. (2010) for gac juice powder. Higher (b)
maltodextrin level and higher inlet temperature resulted in Maltodextrin 5%; Maltodextrin 7%; Maltodextrin 9%
low a/b value and high hue angle which are in concurrence
with Chen et al. (1995). The probable reason may be that Fig. 3 – Effect of spray drying conditions on (a) Total
spray drying increased the surface area causing rapid pigment phenolic content and (b) free radical scavenging activity of
oxidation (Desobry et al., 1997). Reduced formation of iron amla powder.
tannate complex at high inlet temperature is also speculated
which needs further studies for confirmation. juice powder by Kha et al. (2010). The possible explanation for
the low free radical scavenging activity may be because of the
3.4. Effect of spray drying conditions on total phenolic exposure to higher temperatures which adversely affected the
content of amla powder structure of phenolics causing its break down and/or synthesis
into different forms.
Fig. 3a shows the effect of processing conditions on total phe- Increase in the maltodextrin concentration which itself has
nolic content (TPC) of spray dried powder. Drying temperature no free radical scavenging activity, resulted in lower DPPH
and maltodextrin concentration showed significant effect on scavenging activity. Powders containing 5% maltodextrin level
TPC of spray dried powder. TPC was significantly (p < 0.001) showed significantly higher free radical scavenging activity
reduced when inlet temperature was increased from 125 ◦ C to (dry wt basis) than 7 and 9% of maltodextrin. This may be
175 ◦ C temperature, however above 175 ◦ C there was a reverse the dilution effect of maltodextrin when its concentration was
trend. The reason for increased TPC content in the powder raised. Obtained results contradicted the result of Kha et al.
above 175 ◦ C may be because of the polymerization as well as (2010) who found that on varying the maltodextrin level from
synthesis of polyphenols at 200 ◦ C which increases the total 10 to 20% there was no significant effect on total antioxidant
phenolic content of the powder. activity of gac juice powder.
TPC content of the powders was significantly reduced when
the concentration of maltodextrin was increased from 5 to 9%. 4. Conclusion
This can be explained to be due to the concentration effect of
maltodextrin. The effect of spray drying conditions on physicochemi-
cal properties of amla powder was evaluated. Maltodextrin
3.5. Effect of spray drying conditions on free radical concentration (5–9%) and drying temperature (125–200 ◦ C)
scavenging activity of powder significantly affected moisture content, bulk density, hygro-
scopicity, color attributes, TPC and DPPH radical scavenging
Fig. 3b shows the effect of spray drying conditions on DPPH activity. However WSI was not significantly influenced by vary-
radical scavenging activity of amla powder. Maltodextrin level ing the concentration of maltodextrin or inlet temperature.
and drying temperature showed statistically significant effect The developed spray dried powder showed excellent water
(p < 0.01) on the DPPH radical scavenging activity of the pow- solubility that is essential for reconstitution. The amla juice
der. DPPH free radical scavenging activity of the powder was powder dried at 125 ◦ C temperature and maltodextrin concen-
significantly affected by increased inlet temperature. Overall, tration of 5% showed better retention of phenolic content and
on increasing the inlet temperature from 125 ◦ C to 200 ◦ C a free radical scavenging activity but due to excessive stickiness
significant decrease in DPPH radical scavenging activity was of the powder can not be recommended as optimum product.
observed. Similar results were observed in spray dried gac Amla juice powder dried at 175 ◦ C and 7% maltodextrin was
258 food and bioproducts processing 9 2 ( 2 0 1 4 ) 252–258
adequately effective to produce powder with less hygroscop- officinalis) and turmeric (Curcuma longa). J. Food Comp. Anal. 19
icity, acceptable color in terms of L, a and b and potent free (5), 446–452.
radical scavenging activity. Spray dried amla juice powder thus Kha, C.T., Nguyen, H.M., Roach, D.P., 2010. Effects of spray drying
conditions on the physicochemical and antioxidant
can be made into a health promoting reconstituted drink.
properties of the Gac (Momordica cochinchinensis) fruit aril
powder. J. Food Eng. 98, 385–392.
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