Medicinal chemistry II (Practical) Pharm.

Hamza alfunini

Vitamin B12 Assay

 Generic Name : vitamin B12 drug

 Chemical structure :
C63H88 CON14O14P

 Percentage limit :

95-110%

 pharmaceutical uses :

………………………… , …………………….

 Material and Tools :

Material Tools
-Drug - Balance
- D.W - Beaker
- spectrophotometer

 The Procedures :

1- Take 2ML of Vit B12 and Add it to 20 ml of D.W and complete the volume
to 100ml .
2- Measure the absorbance at wl= 334
3- Calculate the % content of drug .

 Calculation :

𝐶.𝑃
% Content of drug =𝐶.𝑡ℎ × 100
𝐴
c.p = 𝐴