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  • Determination of Non-Ionic Surfactants Using HPLC: Method

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    This document describes a method for determining non-ionic surfactants using HPLC. It uses a C18 column with a mobile phase of 90:10 methanol/water at a flow rate of 1.5 ml/min. Detection is via refractive index. The method can separate 12 different non-ionic surfactants including polyethylene glycols, polyoxyethylene sorbitane monolaurate, and lauryl alcohol polyglycol ether.

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    Determinación de surfactantes por hplc

    Original Title

    vev0028j_short_application

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    This document describes a method for determining non-ionic surfactants using HPLC. It uses a C18 column with a mobile phase of 90:10 methanol/water at a flow rate of 1.5 ml/min. Detection is via refractive index. The method can separate 12 different non-ionic surfactants including polyethylene glycols, polyoxyethylene sorbitane monolaurate, and lauryl alcohol polyglycol ether.

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    © All Rights Reserved
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    17 views1 page

    Determination of Non-Ionic Surfactants Using HPLC: Method

    Uploaded by

    str_ila
    This document describes a method for determining non-ionic surfactants using HPLC. It uses a C18 column with a mobile phase of 90:10 methanol/water at a flow rate of 1.5 ml/min. Detection is via refractive index. The method can separate 12 different non-ionic surfactants including polyethylene glycols, polyoxyethylene sorbitane monolaurate, and lauryl alcohol polyglycol ether.

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    © All Rights Reserved
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    Determination of non-ionic Surfactants using HPLC

    Method VEV0028J
    HPLC RP Mode

    Column: Eurospher 100-5 C18, 250 x 4.0 mm ID Order No. 25DE181ESJ

    Phase: Eurospher 100-5 C18

    Conditions: Eluent: MeOH / Water (90:10)


    Gradient: isocratic
    Flow rate: 1.5 ml/min
    Temperature: 25 °C
    Volume: 20 µl

    Detection: Refractive Index (RI)

    Substances: Polyethylene glycol, Polyoxyethylene sorbitane monolaurate, Nonylphenol


    polyglycol ether, Laurylalcohol, Laurylalcohol polyglycol ether, Oleic acid
    diethanolamide, Oleic amide polyglycol ether, Myristylalcohol, Myristyl alcohol
    polyglycol ether, Palmitic acid polyglycol ester, Polyoxyethylene glycerol
    monostearate, Stearic acid polyglycol ester

    Keywords: Surfactants

    Chromatogram: 1
    1. Polyethylene glycols
    2. Polyoxyethylene sorbitane monolaurate
    3. Nonylphenol polyglycol ether
    4. Lauryl alcohol 3
    5. Lauryl alcohol polyglycol ether 6
    6. Oleic acid diethanolamide
    7. Oleic amide polyglycol ether 5
    8. Myristyl alcohol
    9. Myristyl alcohol polyglycol ether 8
    10. Palmitic acid polyglycol ester 4 7
    11. Polyoxyethylene glycerol monostearate
    12. Stearic acid polyglycol ester. 2 10
    12
    9 11

    0 5 10 15 min

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